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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB114
Library VF
(Link to library)
Clone ID VFB114
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14954-1
Original site URL
Representative seq. ID VFB114P
(Link to Original site)
Representative DNA sequence 
>VFB114 (VFB114Q) /CSM/VF/VFB1-A/VFB114Q.Seq.d/
TAAATTTTTTTTTTAAATGATNAAAATGATAAAAAAAGTGCATTAATAACATTATTTTTA
GTTTCACTAATTTTGGGTGTTTCTTTATCCCAAAAACCNTTTTATTGCCAAGCACCAGAA
CCAACACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAGA
CATGGTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTGT
AATTTAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGTT
GATAGATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTCA
GACGGTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACGT
CAACTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATCA
ACCATTTGGGTTCGTTCAACCGATGTACCAAGAACCATTCAATCGGTTXXXXXXXXXXTG
AAAATATGACACCCAAATCCAACTCTTTGCCCAGAGTTGGCTGTTTTGATCGCCAATACT
ACCACCACACCAGAGTGGGGGGAATTCATTACCAACACCACCCAATTAAAAGAGGATGTT
ATGGAAACATTGGGTATCTCTGTATTCCCAGGTTGGAGTTCATTAATGGATTTATTCTTT
GCAACTCAATGTCACGATTTCCCATTACCAGAGGGTGTCACTCAAGATATGGTCACTCAA
GTCTATGAAGCTGCCTATTGGCAATACCAATATCAACTCTCTTTCCCAATGATTGCTCGT
CTTGGTATGTCTACTTTCTTAGAGGAGGTAGTCGATAATATTAGAGCTTTTGTTAATGGT
ACTTCCTCTGTCAAATATATCGTTTTCTCTGGTCATGATGATTCAGTTGGTCCATTCACT
AATTTATTTGGTCTCATGAAAGAATGGCCACCTTATGCTTCACATGTTGAACTTGAATTG
TGGTCTGATGAAAAAGATAATTATTTCCTCCAATTTAAATTTAATGGTCAAAGTTATACA
TTAAATGGTTGTGAAGATGTTATGTGCCCAATTGATTCATTCTTTGAAACTGCATACTCA
ATTTTAGTTCCAAATTATGCTGATGCATGTTCAAATTCAACAANACATTTAATTTAACAA
AAAAACCCTCCGATTTCT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*IFFLNDXNDKKSALITLFLVSLILGVSLSQKPFYCQAPEPTPSLNTDGLTLKMVQILTR
HGDRTPLYSTLKPTMNTWDCNLGWLMVSSLNNVPGAATDVDRLFRKVYMPNREYFPGNCS
DGQLTSLGFQQHLQLGQSLRQLYVDKYELLPSELSVDAASTIWVRSTDVPRTIQSV---

---ENMTPKSNSLPRVGCFDRQYYHHTRVGGIHYQHHPIKRGCYGNIGYLCIPRLEFING
FILCNSMSRFPITRGCHSRYGHSSL*scllaipistlfpndcsswyvyflrggsr*y*sf
c*wyflcqiyrflws**fswsih*fiwshermatlcftc*t*ivv**kr*lfppi*i*ws
klyikwl*rcyvpn*fil*ncilnfssklc*cmfkfnxtfnltkkpsdf
Translated Amino Acid sequence (All Frames) 
Frame A:
*IFFLNDXNDKKSALITLFLVSLILGVSLSQKPFYCQAPEPTPSLNTDGLTLKMVQILTR
HGDRTPLYSTLKPTMNTWDCNLGWLMVSSLNNVPGAATDVDRLFRKVYMPNREYFPGNCS
DGQLTSLGFQQHLQLGQSLRQLYVDKYELLPSELSVDAASTIWVRSTDVPRTIQSV---

---*kydtqiqlfaqswlf*spilpphqsggnslptppn*krmlwkhwvslysqvgvh*w
iyslqlnvtishyqrvslkiwslksmklpigntninslsq*llvlvclls*rr*siilel
llmvlplsnisfslvmmiqlvhsliylvs*knghlmlhmlnlncglmkkiiissnlnlmv
kvih*mvvkmlcaqlihslklhtqf*fqimlmhvqiqqxi*fnkktlrf

Frame B:
kfff*mxkmikkvh**hyf*fh*fwvflypknxfiakhqnqhqv*iqmd*h*rwykf*qd
mviehhcivh*nqq*ihgivi*dg*wyqv*imyqeqqqmlidylekficqienisqvivq
tvn*phldsnniyn*vkvyvnstlinmnyyqvnyqwmqhqpfgfvqpmyqepfnr---

---ENMTPKSNSLPRVGCFDRQYYHHTRVGGIHYQHHPIKRGCYGNIGYLCIPRLEFING
FILCNSMSRFPITRGCHSRYGHSSL*scllaipistlfpndcsswyvyflrggsr*y*sf
c*wyflcqiyrflws**fswsih*fiwshermatlcftc*t*ivv**kr*lfppi*i*ws
klyikwl*rcyvpn*fil*ncilnfssklc*cmfkfnxtfnltkkpsdf

Frame C:
nfffk*xk**kkcinniifsftnfgcffipktxllpstrtntkfkyrwinikdgtnfnkt
w**ntiv*yiktnneymgl*frmvngikfk*ctrssnrc**ii*kslyak*rifpr*lfr
rsidltwipttftirskftstlr**i*iitk*iisgcsinhlgsfnrctknhsig---

---ki*hpnptlcpelavlianttttpewgefitnttqlkedvmetlgisvfpgwsslmd
lffatqchdfplpegvtqdmvtqvyeaaywqyqyqlsfpmiarlgmstfleevvdniraf
vngtssvkyivfsghddsvgpftnlfglmkewppyashvelelwsdekdnyflqfkfngq
sytlngcedvmcpidsffetaysilvpnyadacsnstxhli*qknppis
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB114 (VFB114Q) /CSM/VF/VFB1-A/VFB114Q.Seq.d/                       2230   0.0
CFA225 (CFA225Q) /CSM/CF/CFA2-B/CFA225Q.Seq.d/                       1287   0.0
VFB675 (VFB675Q) /CSM/VF/VFB6-D/VFB675Q.Seq.d/                       1273   0.0
VFB228 (VFB228Q) /CSM/VF/VFB2-B/VFB228Q.Seq.d/                       1273   0.0
AFO855 (AFO855Q) /CSM/AF/AFO8-C/AFO855Q.Seq.d/                       1273   0.0
VFM810 (VFM810Q) /CSM/VF/VFM8-A/VFM810Q.Seq.d/                       1263   0.0
VFG714 (VFG714Q) /CSM/VF/VFG7-A/VFG714Q.Seq.d/                       1263   0.0
VFG445 (VFG445Q) /CSM/VF/VFG4-B/VFG445Q.Seq.d/                       1263   0.0
SFI824 (SFI824Q) /CSM/SF/SFI8-A/SFI824Q.Seq.d/                       1263   0.0
CHD582 (CHD582Q) /CSM/CH/CHD5-D/CHD582Q.Seq.d/                       1263   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BH164673|BH164673.1 ENTTE80TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  0.58  2
AZ544963|AZ544963.1 ENTEB40TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  0.58  2
AC144964|AC144964.1 Silurana tropicalis clone ISB-343A1, WORKING DRAFT SEQUENCE, 5 unordered pieces.    40  1.9  3
AC117184|AC117184.2 Mus musculus chromosome UNK clone RP23-37L10, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44  4.1  1
AC093321|AC093321.1 Homo sapiens chromosome UNK clone RP11-225G3, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  4.1  1
AC092792|AC092792.2 Homo sapiens chromosome UNK clone RP11-806O14, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44  4.1  1
BH151597|BH151597.1 ENTPW89TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44  4.1  1
AC107539|AC107539.5 Rattus norvegicus clone CH230-214F9, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  4.1  1
AC132034|AC132034.4 Rattus norvegicus clone CH230-401A9, WORKING DRAFT SEQUENCE, 4 unordered pieces.    44  4.1  1
BV062901|BV062901.1 S212P6423FB4.T0 CZECHII/Ei Mus musculus STS genomic, sequence tagged site.    44  4.1  1
dna update 2003. 8.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54PR9) RecName: Full=Counting factor 60; Flags: Precursor;  &D...   757   0.0
BC125717_1(BC125717|pid:none) Xenopus tropicalis hypothetical pr...    99   2e-19
BC154980_1(BC154980|pid:none) Xenopus laevis hypothetical protei...    98   5e-19
AF299349_1(AF299349|pid:none) Legionella pneumophila major acid ...    92   3e-17
CR628336_1123(CR628336|pid:none) Legionella pneumophila str. Par...    90   2e-16
(Q54P71) RecName: Full=Probable acid phosphatase DDB_G0284755;  ...    88   7e-16
CR382131_1106(CR382131|pid:none) Yarrowia lipolytica strain CLIB...    87   9e-16
(Q8BP40) RecName: Full=Lysophosphatidic acid phosphatase type 6;...    86   3e-15
AM270193_80(AM270193|pid:none) Aspergillus niger contig An09c004...    85   6e-15
AM920431_449(AM920431|pid:none) Penicillium chrysogenum Wisconsi...    85   6e-15
protein update 2009. 6.17
PSORT 

 psg: 0.97  gvh: 0.80  alm: 0.40  top: 0.47  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VFB114 is exc
5' end seq. ID VFB114F
5' end seq. 
>VFB114F.Seq
TAAATTTTTTTTTTAAATGATNAAAATGATAAAAAAAGTGCATTAATAACATTATTTTTA
GTTTCACTAATTTTGGGTGTTTCTTTATCCCAAAAACCNTTTTATTGCCAAGCACCAGAA
CCAACACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAGA
CATGGTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTGT
AATTTAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGTT
GATAGATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTCA
GACGGTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACGT
CAACTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATCA
ACCATTTGGGTTCGTTCAACCGATGTACCAAGAACCATTCAATCGGTT----------
Length of 5' end seq. 528
3' end seq. ID VFB114Z
3' end seq. 
>VFB114Z.Seq
----------TGAAAATATGACACCCAAATCCAACTCTTTGCCCAGAGTTGGCTGTTTTG
ATCGCCAATACTACCACCACACCAGAGTGGGGGGAATTCATTACCAACACCACCCAATTA
AAAGAGGATGTTATGGAAACATTGGGTATCTCTGTATTCCCAGGTTGGAGTTCATTAATG
GATTTATTCTTTGCAACTCAATGTCACGATTTCCCATTACCAGAGGGTGTCACTCAAGAT
ATGGTCACTCAAGTCTATGAAGCTGCCTATTGGCAATACCAATATCAACTCTCTTTCCCA
ATGATTGCTCGTCTTGGTATGTCTACTTTCTTAGAGGAGGTAGTCGATAATATTAGAGCT
TTTGTTAATGGTACTTCCTCTGTCAAATATATCGTTTTCTCTGGTCATGATGATTCAGTT
GGTCCATTCACTAATTTATTTGGTCTCATGAAAGAATGGCCACCTTATGCTTCACATGTT
GAACTTGAATTGTGGTCTGATGAAAAAGATAATTATTTCCTCCAATTTAAATTTAATGGT
CAAAGTTATACATTAAATGGTTGTGAAGATGTTATGTGCCCAATTGATTCATTCTTTGAA
ACTGCATACTCAATTTTAGTTCCAAATTATGCTGATGCATGTTCAAATTCAACAANACAT
TTAATTTAACAAAAAAACCCTCCGATTTCT
Length of 3' end seq. 680
Connected seq. ID VFB114P
Connected seq. 
>VFB114P.Seq
TAAATTTTTTTTTTAAATGATNAAAATGATAAAAAAAGTGCATTAATAACATTATTTTTA
GTTTCACTAATTTTGGGTGTTTCTTTATCCCAAAAACCNTTTTATTGCCAAGCACCAGAA
CCAACACCAAGTTTAAATACAGATGGATTAACATTAAAGATGGTACAAATTTTAACAAGA
CATGGTGATAGAACACCATTGTATAGTACATTAAAACCAACAATGAATACATGGGATTGT
AATTTAGGATGGTTAATGGTATCAAGTTTAAATAATGTACCAGGAGCAGCAACAGATGTT
GATAGATTATTTAGAAAAGTTTATATGCCAAATAGAGAATATTTCCCAGGTAATTGTTCA
GACGGTCAATTGACCTCACTTGGATTCCAACAACATTTACAATTAGGTCAAAGTTTACGT
CAACTCTACGTTGATAAATATGAATTATTACCAAGTGAATTATCAGTGGATGCAGCATCA
ACCATTTGGGTTCGTTCAACCGATGTACCAAGAACCATTCAATCGGTT----------TG
AAAATATGACACCCAAATCCAACTCTTTGCCCAGAGTTGGCTGTTTTGATCGCCAATACT
ACCACCACACCAGAGTGGGGGGAATTCATTACCAACACCACCCAATTAAAAGAGGATGTT
ATGGAAACATTGGGTATCTCTGTATTCCCAGGTTGGAGTTCATTAATGGATTTATTCTTT
GCAACTCAATGTCACGATTTCCCATTACCAGAGGGTGTCACTCAAGATATGGTCACTCAA
GTCTATGAAGCTGCCTATTGGCAATACCAATATCAACTCTCTTTCCCAATGATTGCTCGT
CTTGGTATGTCTACTTTCTTAGAGGAGGTAGTCGATAATATTAGAGCTTTTGTTAATGGT
ACTTCCTCTGTCAAATATATCGTTTTCTCTGGTCATGATGATTCAGTTGGTCCATTCACT
AATTTATTTGGTCTCATGAAAGAATGGCCACCTTATGCTTCACATGTTGAACTTGAATTG
TGGTCTGATGAAAAAGATAATTATTTCCTCCAATTTAAATTTAATGGTCAAAGTTATACA
TTAAATGGTTGTGAAGATGTTATGTGCCCAATTGATTCATTCTTTGAAACTGCATACTCA
ATTTTAGTTCCAAATTATGCTGATGCATGTTCAAATTCAACAANACATTTAATTTAACAA
AAAAACCCTCCGATTTCT
Length of connected seq. 1208
Full length Seq ID -
Full length Seq. -
Length of full length seq. -