NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFB101
Library VF
(Link to library)
Clone ID VFB101
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16272-1
Original site URL
Representative seq. ID VFB101P
(Link to Original site)
Representative DNA sequence 
>VFB101 (VFB101Q) /CSM/VF/VFB1-A/VFB101Q.Seq.d/
TCGTAAATTTGAAGCTCCACGTCACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGC
CAGACATCAAGGTAAAGTCAAGTCATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTT
AACTGCATTCATGGGTTACAAAGCTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACC
AGGTTCAAAGATGCACAAAAAAGAAATCGTTGAAGCCGTCACCATCATTGAATGTCCACC
AATGTACATTGTTGGTTTAGTTGGTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAA
AACCGTCTGGGCTCAACATTTAAGTGATAACTTCCGTAGAAGATTATACAAAAACTGGTA
CAAATCAAAATCAAAGAAAGCTTTCACCAAATACGTTAAACAATACGAAACTGAAGAAGG
TAAAAAATCAATTGAAGCCTCXXXXXXXXXXTTAATCGATGTTATCGGTGTAACCAAAGG
TAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGTAAATTACCAAGAAAGACCCA
TAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCATCCATCCCGTGTTAGTACCAC
CGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTCGAAAGAAACAAGAAGATTTA
CAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATCTCAACCGGTAAGACTGAATT
CGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTCGCTCACTACGGTATGGTTAG
ACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTT
AAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATT
CATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAA
ATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAATTCAATAATTAGATTTCAGA
GAGTATCAAACA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
RKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGMTHVVRDLEKP
GSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDNFRRRLYKNWY
KSKSKKAFTKYVKQYETEEGKKSIEA---

---LIDVIGVTKGKGFNGVIKRWGVRKLPRKTHKGLRKVACIGAWHPSRVSTTVPRAGQL
GYHHRVERNKKIYRIGQAQPEDGKQISTGKTEFDLTEKTINPMGGFAHYGMVRHEFLMLK
GCVAGPRKRALTLRKSITTQTGRAALEKITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKS
RSTKA*fnn*isesik
Translated Amino Acid sequence (All Frames) 
Frame A:
s*i*sstsr*srfqtkkesrqtsr*sqvipkg*pypksplncihglqswydsrcqrfrkt
rfkdaqkrnr*srhhh*mstnvhcwfswlc*nrsrsqnlqnrlgstfk**lp*kiiqklv
qikikesfhqir*tirn*rr*kin*sl---

---LIDVIGVTKGKGFNGVIKRWGVRKLPRKTHKGLRKVACIGAWHPSRVSTTVPRAGQL
GYHHRVERNKKIYRIGQAQPEDGKQISTGKTEFDLTEKTINPMGGFAHYGMVRHEFLMLK
GCVAGPRKRALTLRKSITTQTGRAALEKITLKFIDTSSKFGHGLHQTAEDKTKYFGVKKS
RSTKA*fnn*isesik

Frame B:
RKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKAGMTHVVRDLEKP
GSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLSDNFRRRLYKNWY
KSKSKKAFTKYVKQYETEEGKKSIEA---

---*smlsv*pkvkvsmvslkdgvsvnyqerpikv*erlpvlvhgihpvlvppsqelvns
vtiivsketrrftesvklnqktvnksqpvrlnsi*lkrlsiqwvvslttvwldmns*clk
vvllvqerep*p*ervsplklvvlpsrkslsnssippaslvtvfikplkirlnisasrnq
dqlrlnsiirfqrvsn

Frame C:
vnlklhvtvisvsdqereppdikvksshsqrmtvpkkst*lhswvtklv*ltlsei*knq
vqrctkkkslkpspslnvhqctllv*lvmlkplkvskptkpsglni*vitsvedytktgt
nqnqrklspntlnntklkkvknqlkp---

---nrcyrcnqr*rfqwch*kmgcp*itkkdp*rfkkgclywcmasipc*yhrpkswstr
lpsscrkkqedlqnrssstrrr*tnlnr*d*irfn*kdyqsngwfrslryg*t*ilna*r
lccwskkeslnlkkeyhhsnwsccprenhsqihrylqqvwsrsssnr*r*d*ifrrqeik
in*gliq*ldfreyqt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFB101 (VFB101Q) /CSM/VF/VFB1-A/VFB101Q.Seq.d/                       1986   0.0
VFO878 (VFO878Q) /CSM/VF/VFO8-D/VFO878Q.Seq.d/                       1144   0.0
VFO722 (VFO722Q) /CSM/VF/VFO7-A/VFO722Q.Seq.d/                       1136   0.0
VFA851 (VFA851Q) /CSM/VF/VFA8-C/VFA851Q.Seq.d/                       1130   0.0
VFA644 (VFA644Q) /CSM/VF/VFA6-B/VFA644Q.Seq.d/                       1130   0.0
CFB715 (CFB715Q) /CSM/CF/CFB7-A/CFB715Q.Seq.d/                       1130   0.0
VFK363 (VFK363Q) /CSM/VF/VFK3-C/VFK363Q.Seq.d/                       1128   0.0
VFA839 (VFA839Q) /CSM/VF/VFA8-B/VFA839Q.Seq.d/                       1128   0.0
VFA602 (VFA602Q) /CSM/VF/VFA6-A/VFA602Q.Seq.d/                       1128   0.0
SFC773 (SFC773Q) /CSM/SF/SFC7-D/SFC773Q.Seq.d/                       1124   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L08391|L08391.1 Dictyostelium discoideum ribosomal protein (L3) gene, complete cds.   874  0.0  4
AX489150|AX489150.1 Sequence 6450 from Patent WO02053728.         78  2e-43  9
Z74971|Z74971.1 S.cerevisiae chromosome XV reading frame ORF YOR063w.    70  1e-35  8
J01351|J01351.1 S.cerevisiae ribosomal protein L3 (tcm1) gene.    62  6e-35  8
Z70678|Z70678.1 S.cerevisiae chromosome XV DNA, 54.7 kb region.    70  1e-27  9
AL424946|AL424946.1 clone BA0AB002D01 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    66  1e-19  5
AX765804|AX765804.1 Sequence 13 from Patent WO03008440.           74  2e-19  4
AY052743|AY052743.1 Arabidopsis thaliana At1g43170/F1I21_18 mRNA, complete cds.    74  2e-19  4
AF361101|AF361101.1 Arabidopsis thaliana putative L3 cytoplasmic ribosomal protein (F1I21.L3) mRNA, complete cds.    74  2e-19  4
AY057487|AY057487.1 Arabidopsis thaliana At1g43170/F1I21_18 mRNA, complete cds.    74  3e-19  4
dna update 2003. 8.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z49936_3(Z49936|pid:none) Caenorhabditis elegans Cosmid F13B10, ...   421   e-116
AK092695_1(AK092695|pid:none) Homo sapiens cDNA FLJ35376 fis, cl...   415   e-114
AJ720292_1(AJ720292|pid:none) Gallus gallus mRNA for hypothetica...   400   e-110
EF070522_1(EF070522|pid:none) Maconellicoccus hirsutus clone WHM...   399   e-110
BC158279_1(BC158279|pid:none) Xenopus tropicalis hypothetical pr...   397   e-109
AK151142_1(AK151142|pid:none) Mus musculus bone marrow macrophag...   397   e-109
(P39872) RecName: Full=60S ribosomal protein L3;  &BC102253_1(BC...   397   e-109
AK151057_1(AK151057|pid:none) Mus musculus bone marrow macrophag...   397   e-109
(P21531) RecName: Full=60S ribosomal protein L3; AltName: Full=L...   396   e-109
(P39023) RecName: Full=60S ribosomal protein L3; AltName: Full=H...   396   e-109
protein update 2009. 6.17
PSORT 

 psg: 0.63  gvh: 0.21  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.05
 nuc: 0.39  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VFB101 is nuc
5' end seq. ID VFB101F
5' end seq. 
>VFB101F.Seq
TCGTAAATTTGAAGCTCCACGTCACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGC
CAGACATCAAGGTAAAGTCAAGTCATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTT
AACTGCATTCATGGGTTACAAAGCTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACC
AGGTTCAAAGATGCACAAAAAAGAAATCGTTGAAGCCGTCACCATCATTGAATGTCCACC
AATGTACATTGTTGGTTTAGTTGGTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAA
AACCGTCTGGGCTCAACATTTAAGTGATAACTTCCGTAGAAGATTATACAAAAACTGGTA
CAAATCAAAATCAAAGAAAGCTTTCACCAAATACGTTAAACAATACGAAACTGAAGAAGG
TAAAAAATCAATTGAAGCCTC----------
Length of 5' end seq. 441
3' end seq. ID VFB101Z
3' end seq. 
>VFB101Z.Seq
----------TTAATCGATGTTATCGGTGTAACCAAAGGTAAAGGTTTCAATGGTGTCAT
TAAAAGATGGGGTGTCCGTAAATTACCAAGAAAGACCCATAAAGGTTTAAGAAAGGTTGC
CTGTATTGGTGCATGGCATCCATCCCGTGTTAGTACCACCGTCCCAAGAGCTGGTCAACT
CGGTTACCATCATCGTGTCGAAAGAAACAAGAAGATTTACAGAATCGGTCAAGCTCAACC
AGAAGACGGTAAACAAATCTCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTAT
CAATCCAATGGGTGGTTTCGCTCACTACGGTATGGTTAGACATGAATTCTTAATGCTTAA
AGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCA
AACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTT
TGGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAAATATTTCGGCGTCAAGAAATC
AAGATCAACTAAGGCTTAATTCAATAATTAGATTTCAGAGAGTATCAAACA
Length of 3' end seq. 581
Connected seq. ID VFB101P
Connected seq. 
>VFB101P.Seq
TCGTAAATTTGAAGCTCCACGTCACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGC
CAGACATCAAGGTAAAGTCAAGTCATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTT
AACTGCATTCATGGGTTACAAAGCTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACC
AGGTTCAAAGATGCACAAAAAAGAAATCGTTGAAGCCGTCACCATCATTGAATGTCCACC
AATGTACATTGTTGGTTTAGTTGGTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAA
AACCGTCTGGGCTCAACATTTAAGTGATAACTTCCGTAGAAGATTATACAAAAACTGGTA
CAAATCAAAATCAAAGAAAGCTTTCACCAAATACGTTAAACAATACGAAACTGAAGAAGG
TAAAAAATCAATTGAAGCCTC----------TTAATCGATGTTATCGGTGTAACCAAAGG
TAAAGGTTTCAATGGTGTCATTAAAAGATGGGGTGTCCGTAAATTACCAAGAAAGACCCA
TAAAGGTTTAAGAAAGGTTGCCTGTATTGGTGCATGGCATCCATCCCGTGTTAGTACCAC
CGTCCCAAGAGCTGGTCAACTCGGTTACCATCATCGTGTCGAAAGAAACAAGAAGATTTA
CAGAATCGGTCAAGCTCAACCAGAAGACGGTAAACAAATCTCAACCGGTAAGACTGAATT
CGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTCGCTCACTACGGTATGGTTAG
ACATGAATTCTTAATGCTTAAAGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTT
AAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATT
CATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCATCAAACCGCTGAAGATAAGACTAA
ATATTTCGGCGTCAAGAAATCAAGATCAACTAAGGCTTAATTCAATAATTAGATTTCAGA
GAGTATCAAACA
Length of connected seq. 1022
Full length Seq ID -
Full length Seq. -
Length of full length seq. -