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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA820
Library VF
(Link to library)
Clone ID VFA820
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFA820P
(Link to Original site)
Representative DNA sequence 
>VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGT
GAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCAC
GTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAA
ACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATAACAAAGAGATCCAAGCAGGTAACCCTCGCTGATGATTXXXXXXXXXXTCC
CAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACG
GTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGA
AAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCNTTAATTGATAACCCAGAAT
ACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAATTAT
GGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAG
CTAAAGATTTNTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAANAAAATGTTTG
ACGACCTCGAAGCCGCCAAAGCTGAANANANAAANAAAGCTGACGAAAAATTAGCTGCCG
AAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAG
AAGATTTAGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkm
vnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkli
vvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklk
pin*LINTLWSFHQITPTKYSSITKRSKQVTLADD---

---PASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWXXPLID
NPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEXAKDXXEKTFVANQEAEX
KMFDDLEAAKAEXXXKADEKLAAEKAAEKEAEEADEEEEEVAEEDL
Translated Amino Acid sequence (All Frames) 
Frame A:
iynyiffv**krkntnmrlllcliflvfvfnfalstvhfkdtfdndwesrwvvsdwhked
gksgklvhtagkwfgdenqkgiqtsedarfyavsakfpsfsnkgkdlvlqytvkneqkvd
cggsyikllpskldqsafdgeseysimfgpdvcgaskrvhvilnykgknhlikkeinkve
tdqlthqytlvispdntykvlvdnkeiqagnpr**---

---sqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngxxx*l
itqntlktmnstslmisxlsvsnygklnlvpslti*ssptlsxklkixxkrpllptkkpk
xkclttskppklxxxxkltkn*lpkkllkkkpkklmkkkkkslkki*

Frame B:
fiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikkm
vnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkli
vvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstklk
pin*LINTLWSFHQITPTKYSSITKRSKQVTLADD---

---psfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpxn*
*prir*rq*tlpl**srxyrfrimas*iwfhl*qydrxrlcrxs*rfx*kdlccqprsrk
xnv*rprsrqs*xxkxs*rkiscrksc*krsrrs**rrrrsr*rrfs

Frame C:
l*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rrw
*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*l
wwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*n
rsinssihfghftr*hlqstrr*qrdpsr*pslmi---

---PASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWXXPLID
NPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEXAKDXXEKTFVANQEAEX
KMFDDLEAAKAEXXXKADEKLAAEKAAEKEAEEADEEEEEVAEEDL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/                       1782   0.0
AHA115 (AHA115Q) /CSM/AH/AHA1-A/AHA115Q.Seq.d/                       1092   0.0
AFM264 (AFM264Q) /CSM/AF/AFM2-C/AFM264Q.Seq.d/                       1092   0.0
AFO205 (AFO205Q) /CSM/AF/AFO2-A/AFO205Q.Seq.d/                       1084   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1076   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1076   0.0
SLE570 (SLE570Q) /CSM/SL/SLE5-C/SLE570Q.Seq.d/                       1076   0.0
SLE160 (SLE160Q) /CSM/SL/SLE1-C/SLE160Q.Seq.d/                       1076   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1076   0.0
AFK716 (AFK716Q) /CSM/AF/AFK7-A/AFK716Q.Seq.d/                       1076   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1059  0.0  5
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  9e-24  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    72  2e-14  2
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  7e-11  2
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  4e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  4e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  4e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  4e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  4e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  4e-09  1
dna update 2003. 8.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   370   e-101
BT072764_1(BT072764|pid:none) Salmo salar clone ssal-rgf-539-230...   209   1e-81
L24159_1(L24159|pid:none) Schistosoma mansoni calreticulin gene,...   167   6e-69
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   231   4e-59
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   230   6e-59
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   230   6e-59
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   230   8e-59
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   229   1e-58
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   229   2e-58
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   228   3e-58
protein update 2009. 6.16
PSORT 

 psg: 0.87  gvh: 0.49  alm: 0.47  top: 0.70  tms: 0.00  mit: 0.42  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 28.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFA820 is mit
5' end seq. ID VFA820F
5' end seq. 
>VFA820F.Seq
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGT
GAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCAC
GTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAA
ACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATAACAAAGAGATCCAAGCAGGTAACCCTCGCTGATGATT----------
Length of 5' end seq. 647
3' end seq. ID VFA820Z
3' end seq. 
>VFA820Z.Seq
----------TCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAAT
GAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAA
TGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCNTTAATT
GATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATC
GGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGAC
TCTGTCGAANAAGCTAAAGATTTNTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAA
AANAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAANANANAAANAAAGCTGACGAA
AAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAA
GAAGTCGCTGAAGAAGATTTAGT
Length of 3' end seq. 493
Connected seq. ID VFA820P
Connected seq. 
>VFA820P.Seq
ATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTTTA
TTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCAAA
GATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAGAT
GGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAAAA
GGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTTTC
AGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTGAT
TGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATGGT
GAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTCAC
GTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTGAA
ACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAGTA
CTCGTCGATAACAAAGAGATCCAAGCAGGTAACCCTCGCTGATGATT----------TCC
CAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACG
GTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGA
AAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCNTTAATTGATAACCCAGAAT
ACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGNGCTATCGGTTTCGAATTAT
GGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAG
CTAAAGATTTNTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAANAAAATGTTTG
ACGACCTCGAAGCCGCCAAAGCTGAANANANAAANAAAGCTGACGAAAAATTAGCTGCCG
AAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAG
AAGATTTAGT
Length of connected seq. 1140
Full length Seq ID -
Full length Seq. -
Length of full length seq. -