>VFA764 (VFA764Q) /CSM/VF/VFA7-C/VFA764Q.Seq.d/
XXXXXXXXXXTCAAAGGTATTCGTCCAGAACAAACTAAAAACCTCGCTGAGAAATTAGGT
TTCACCGGTGAGAAAGCAAAGATCGCTCAACAACAAATGGAAAACCTCTATCAATTATTC
ATCAAGAGTGATGCAACTCAAGTCGAGATTAACCCATTCGCTGAAACCACCGATGGTCAA
GTCATTTGTATGGATGCTAAAATCAATTTCGATGACAATGCCTCATTCCGTCAAAAGGAG
ATTTTCGAAATGAGAGATACCGCCGAAGAGGATCCACGTGAGGTTGAAGCTGGTAAATTT
GGTCTCAATTACATTGGTCTCGATGGTAACATTGGTTGTATGGTTAATGGTGCTGGTTTG
GCTATGGCCACCATGGATATCATTAAATTAAAGGGTGGTATCCCTGCTAATTTCCTCGAT
GTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAGCCTTCAAAATCCTCACTAAAGAT
CCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTGGTATCATGAAATGTGATATCATT
GCCTCTGGTATCGTAAATGCTTCAAAACAAGTTGGTCTTAAAATTCCATTAGTTGTACGT
TTAGAAGGTACAAATGTAAACAT

---KGIRPEQTKNLAEKLGFTGEKAKIAQQQMENLYQLFIKSDATQVEINPFAETTDGQV
ICMDAKINFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLDGNIGCMVNGAGLA
MATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLVNIFGGIMKCDIIA
SGIVNASKQVGLKIPLVVRLEGTNVN

Frame A:
---qryssrtn*kpr*eirfhr*eskdrsttngkplsiihqe*cnssrd*pir*nhrwss
hlygc*nqfr*qclipskgdfrneryrrrgst*g*sw*iwsqlhwsrw*hwlyg*wcwfg
yghhgyh*ikgwypc*fprcrw*cl*tsrh*slqnph*rsks*mftrkhfrwyhem*yhc
lwyrkcfktsws*nsisctfrrykckh


Frame B:
---KGIRPEQTKNLAEKLGFTGEKAKIAQQQMENLYQLFIKSDATQVEINPFAETTDGQV
ICMDAKINFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLDGNIGCMVNGAGLA
MATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLVNIFGGIMKCDIIA
SGIVNASKQVGLKIPLVVRLEGTNVN


Frame C:
---kvfvqnklktslrn*vspvrkqrslnnkwktsinyssrvmqlksrlthslkppmvks
fvwmlksismtmphsvkrrfsk*eippkrihvrlklvnlvsitlvsmvtlvvwlmvlvwl
wppwisln*rvvsllissm*vvvplnkpslkpsksslkiqelnvys*tfsvvs*nvislp
lvs*mlqnklvlkfh*lyv*kvqm*t

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ843 (VFJ843Q) /CSM/VF/VFJ8-B/VFJ843Q.Seq.d/                       1215   0.0
VFJ446 (VFJ446Q) /CSM/VF/VFJ4-B/VFJ446Q.Seq.d/                       1215   0.0
VFF668 (VFF668Q) /CSM/VF/VFF6-C/VFF668Q.Seq.d/                       1215   0.0
VFD829 (VFD829Q) /CSM/VF/VFD8-B/VFD829Q.Seq.d/                       1215   0.0
VFC521 (VFC521Q) /CSM/VF/VFC5-A/VFC521Q.Seq.d/                       1215   0.0
VFA764 (VFA764Q) /CSM/VF/VFA7-C/VFA764Q.Seq.d/                       1215   0.0
SFK777 (SFK777Q) /CSM/SF/SFK7-D/SFK777Q.Seq.d/                       1215   0.0
SFH344 (SFH344Q) /CSM/SF/SFH3-B/SFH344Q.Seq.d/                       1215   0.0
SFC186 (SFC186Q) /CSM/SF/SFC1-D/SFC186Q.Seq.d/                       1215   0.0
AFK346 (AFK346Q) /CSM/AF/AFK3-B/AFK346Q.Seq.d/                       1215   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1215  0.0  1
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    62  5e-26  6
X84222|X84222.2 Neocallimastix frontalis mRNA for beta-succinyl CoA synthetase precursor.    74  8e-22  4
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    74  1e-21  4
AX621832|AX621832.1 Sequence 4795 from Patent WO02094868.         52  8e-20  4
BJ519880|BJ519880.1 Oryzias latipes cDNA clone:MF01SSB014A03, 5' end single read.    70  8e-17  2
BJ532800|BJ532800.1 Oryzias latipes cDNA clone:MF01SSB015A03, 3' end single read.    70  8e-17  2
BJ520215|BJ520215.1 Oryzias latipes cDNA clone:MF01SSB015A03, 5' end single read.    70  8e-17  2
CC820121|CC820121.1 1O0001G05R Oxytricha plasmid UUGC1O library Sterkiella histriomuscorum genomic clone UUGC1O0001G05 R, genomic survey sequence.    84  4e-16  2
BJ532460|BJ532460.1 Oryzias latipes cDNA clone:MF01SSB014A03, 3' end single read.    64  5e-15  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115594_9(AC115594|pid:none) Dictyostelium discoideum chromosom...   283   4e-75
(Q55AI5) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   283   4e-75
BT039713_1(BT039713|pid:none) Zea mays full-length cDNA clone ZM...   276   3e-73
(Q6K9N6) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   276   4e-73
EF530042_1(EF530042|pid:none) Caragana jubata succinyl-CoA ligas...   273   3e-72
(O82662) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   270   2e-71
AY087596_1(AY087596|pid:none) Arabidopsis thaliana clone 36904 m...   268   1e-70
AK221149_1(AK221149|pid:none) Arabidopsis thaliana mRNA for succ...   266   3e-70
DQ200398_1(DQ200398|pid:none) Solanum tuberosum clone 071C05 suc...   265   6e-70
DQ294279_1(DQ294279|pid:none) Solanum tuberosum clone 180E05 suc...   265   6e-70

 psg: 0.65  gvh: 0.34  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VFA764 is cyt

>VFA764Z.Seq
----------TCAAAGGTATTCGTCCAGAACAAACTAAAAACCTCGCTGAGAAATTAGGT
TTCACCGGTGAGAAAGCAAAGATCGCTCAACAACAAATGGAAAACCTCTATCAATTATTC
ATCAAGAGTGATGCAACTCAAGTCGAGATTAACCCATTCGCTGAAACCACCGATGGTCAA
GTCATTTGTATGGATGCTAAAATCAATTTCGATGACAATGCCTCATTCCGTCAAAAGGAG
ATTTTCGAAATGAGAGATACCGCCGAAGAGGATCCACGTGAGGTTGAAGCTGGTAAATTT
GGTCTCAATTACATTGGTCTCGATGGTAACATTGGTTGTATGGTTAATGGTGCTGGTTTG
GCTATGGCCACCATGGATATCATTAAATTAAAGGGTGGTATCCCTGCTAATTTCCTCGAT
GTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAGCCTTCAAAATCCTCACTAAAGAT
CCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTGGTATCATGAAATGTGATATCATT
GCCTCTGGTATCGTAAATGCTTCAAAACAAGTTGGTCTTAAAATTCCATTAGTTGTACGT
TTAGAAGGTACAAATGTAAACAT