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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA717
Library VF
(Link to library)
Clone ID VFA717
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16445-1
Original site URL
Representative seq. ID VFA717E
(Link to Original site)
Representative DNA sequence 
>VFA717 (VFA717Q) /CSM/VF/VFA7-A/VFA717Q.Seq.d/
TACAAATAAAATGAATAAAATTTTAACTCGTAGTTTTAGTACTGGTGCAAATTTCTTTGC
CTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCATTACTGGTGCCAGTGGTCAAATTGG
TTATCAATTATTATTCAGAATTGCCAGTGGTGATATGTTAGGTAAAGATCAACCAATCAT
TCTCCAATGTTTAGAATTACCAGGTGCAATGAACTCATTAAAAGGTGTTTCAATGGAATT
GGATGATTGTGCATTCCCATTGCTCAAAGGTATTGTTCAAAGTGATAAACCAGAGGAAGC
ATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGCTAGTCCAAGAAGCAAGGGTATGGA
GCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTTCTCTGTTCAAGGTAAAGCATTAGA
CAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGTTGTAGGTAACCCAGCCAATACCAA
CGCATTAATTGCCGCCCGTAATGCACCAAATATTGACCCAAAACGTTTCTCTGCCATGAC
TCGTTTAGATCACAACAGAGGTTTAGCTCAATTAGCCGATAAAACTGGTTCAGCCGTAAC
TGATATTGAAAAATTCTGCATTTGGGGTAACCACTCTGCCACCCAATACCCAGATATTAA
CTTTGGTACCGTCAAAGGTAAATCACTCGTAGATACCATCAACGATCAAAAATGGGTTAA
AGATAACTTTATTCCAACCGTTCAACAAAGAGGTGCCGCTATTATTGCTGCCCGTGGTTT
ATCATCAGCTGCCTCTGCTGCTTCAGCTGCCATCGATCACATGAGAGATTGGACCTATGG
TACCAATGGTCAATGGACCTCTATGGCCATTTACTCTGAAGGTGAATATGGTGCCGATAA
AGGTCTCTACTTCTCTTTCCCAGTCATTGTAGATAACAAAGGTAAATACGAAATCGTTAA
AGGTCTTAAACTTGATCAATTCTCTCAAGAACGTTTCGATGCTACTCGTAAAGAATTATT
ATCAGAAAGGATGGTGTTAAAGAAT
sequence update 2001. 6. 9
Translated Amino Acid sequence 
TNKMNKILTRSFSTGANFFASGKGKAPVRVAITGASGQIGYQLLFRIASGDMLGKDQPII
LQCLELPGAMNSLKGVSMELDDCAFPLLKGIVQSDKPEEAFAGADYALLVGASPRSKGME
RGDLLKANAEIFSVQGKALDKSANRDTLRVLVVGNPANTNALIAARNAPNIDPKRFSAMT
RLDHNRGLAQLADKTGSAVTDIEKFCIWGNHSATQYPDINFGTVKGKSLVDTINDQKWVK
DNFIPTVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGQWTSMAIYSEGEYGADK
GLYFSFPVIVDNKGKYEIVKGLKLDQFSQERFDATRKELLSERMVLKN
Translated Amino Acid sequence (All Frames) 
Frame A:
yk*ne*nfns*f*ywckflclw*r*sts*schywcqwsnwlsiiiqncqw*yvr*rstnh
spmfritrcnelikrcfngig*lcipiaqrycsk**trgsirwcrlciiswc*skkqgyg
aw*pfkskc*nflcsr*sirqkc*s*hfksfscr*psqyqrincrp*ctky*pktflchd
sfrsqqrfssisr*nwfsrn*y*kilhlg*plchpipry*lwyrqr*itrryhqrskmg*
r*lysnrstkrcryyccpwfiisclccfschrsherldlwyqwsmdlyghll*r*iwcr*
rslllfpshcr*qr*irnr*rs*t*silsrtfrcys*riiirkdgvke


Frame B:
TNKMNKILTRSFSTGANFFASGKGKAPVRVAITGASGQIGYQLLFRIASGDMLGKDQPII
LQCLELPGAMNSLKGVSMELDDCAFPLLKGIVQSDKPEEAFAGADYALLVGASPRSKGME
RGDLLKANAEIFSVQGKALDKSANRDTLRVLVVGNPANTNALIAARNAPNIDPKRFSAMT
RLDHNRGLAQLADKTGSAVTDIEKFCIWGNHSATQYPDINFGTVKGKSLVDTINDQKWVK
DNFIPTVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGQWTSMAIYSEGEYGADK
GLYFSFPVIVDNKGKYEIVKGLKLDQFSQERFDATRKELLSERMVLKN


Frame C:
qik*ikf*lvvlvlvqislplvkvkhqlelpllvpvvklvinyyselpvvic*vkinqsf
snv*nyqvq*th*kvfqwnwmivhshcskvlfkvinqrkhslvqtmhy*lvlvqearvws
vvtf*kqmlkfslfkvkh*tkvlivtl*ef*l*vtqpipth*lppvmhqiltqnvslp*l
v*ittev*ln*piklvqp*lilknsafgvttlppntqiltlvpskvnhs*ipstiknglk
itlfqpfnkevpllllpvvyhqlplllqlpsit*eigpmvpmvngplwpftlkvnmvpik
vstslsqsl*itkvntkslkvlnlinslknvsmllvknyyqkgwc*r
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA717 (VFA717Q) /CSM/VF/VFA7-A/VFA717Q.Seq.d/                       2072   0.0
VFI317 (VFI317Q) /CSM/VF/VFI3-A/VFI317Q.Seq.d/                       2064   0.0
VFF865 (VFF865Q) /CSM/VF/VFF8-C/VFF865Q.Seq.d/                       2064   0.0
VFF391 (VFF391Q) /CSM/VF/VFF3-D/VFF391Q.Seq.d/                       2064   0.0
CHB451 (CHB451Q) /CSM/CH/CHB4-C/CHB451Q.Seq.d/                       2064   0.0
VFB116 (VFB116Q) /CSM/VF/VFB1-A/VFB116Q.Seq.d/                       2058   0.0
CFE523 (CFE523Q) /CSM/CF/CFE5-A/CFE523Q.Seq.d/                       2058   0.0
VFD687 (VFD687Q) /CSM/VF/VFD6-D/VFD687Q.Seq.d/                       2052   0.0
VFH801 (VFH801Q) /CSM/VF/VFH8-A/VFH801Q.Seq.d/                       2050   0.0
VFD443 (VFD443Q) /CSM/VF/VFD4-B/VFD443Q.Seq.d/                       2050   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX685430|AX685430.1 Sequence 43 from Patent WO02090383.           42  5e-04  3
AF288743|AF288743.1 Trichomonas tenax putative cytosolic malate dehydrogenase (MDH) mRNA, partial cds.    38  5e-04  3
AX067436|AX067436.1 Sequence 11 from Patent WO0078968.            42  0.057  3
U38692|U38692.1 Trichomonas vaginalis cytosolic malate dehydrogenase gene, complete cds.    46  0.88  1
CD766976|CD766976.1 AGENCOURT_14738088 NICHD_Rh_Test1 Macaca mulatta cDNA clone IMAGE:6972628 5', mRNA sequence.    46  0.88  1
CD766800|CD766800.1 AGENCOURT_14708832 NICHD_Rh_Test1 Macaca mulatta cDNA clone IMAGE:6972656 5', mRNA sequence.    46  0.88  1
AF288746|AF288746.2 Hypotrichomonas acosta putative cytosolic malate dehydrogenase (MDH) mRNA, complete cds.    34  1.0  3
BU662722|BU662722.1 cl90b10.z1 Hembase; Erythroid Precursor Cells (LCB:cl library) Homo sapiens cDNA clone cl90b10 5', mRNA sequence.    38  1.2  2
BM393456|BM393456.1 50072-2-1-B11.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    38  1.3  2
AF288748|AF288748.1 Monocercomonas colubrorum putative cytosolic malate dehydrogenase (MDH) mRNA, partial cds.    36  1.7  2
dna update 2003. 8.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54D04) RecName: Full=Probable malate dehydrogenase 2, mitochon...   627   e-178
(Q0ABE6) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   376   e-103
(A3M928) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   362   1e-98
(A6SY47) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   362   2e-98
CP000934_1926(CP000934|pid:none) Cellvibrio japonicus Ueda107, c...   361   3e-98
(Q1LKG0) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   360   3e-98
CP001614_1530(CP001614|pid:none) Teredinibacter turnerae T7901, ...   360   5e-98
CP001053_2776(CP001053|pid:none) Burkholderia phytofirmans PsJN ...   360   6e-98
(Q0K8F5) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   358   1e-97
(Q6F7X1) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   358   1e-97
protein update 2009. 7.10
PSORT 

 psg: 0.87  gvh: 0.46  alm: 0.42  top: 0.47  tms: 0.00  mit: 0.39  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFA717 is mit
5' end seq. ID VFA717F
5' end seq. 
>VFA717F.Seq
TACAAATAAAATGAATAAAATTTTAACTCGTAGTTTTAGTACTGGTGCAAATTTCTTTGC
CTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCATTACTGGTGCCAGTGGTCAAATTGG
TTATCAATTATTATTCAGAATTGCCAGTGGTGATATGTTAGGTAAAGATCAACCAATCAT
TCTCCAATGTTTAGAATTACCAGGTGCAATGAACTCATTAAAAGGTGTTTCAATGGAATT
GGATGATTGTGCATTCCCATTGCTCAAAGGTATTGTTCAAAGTGATAAACCAGAGGAAGC
ATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGCTAGTCCAAGAAGCAAGGGTATGGA
GCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTTCTCTGTTCAAGGTAAAGCATTAGA
CAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGTTGTAGGTAACCCAGCCAATACCAA
CGCATTAATTGCCGCCCGTAATGCACCAAATATTGACCCAAAACGTTTCTCTGCCATGAC
TCGTTTAGATCACAACAGAGGTTTAGCTCAATTAGCCGATAAAACTGGTTCAGCCGTAAC
TGATATTGAAAAATTCTGCATTTGGGGTAACCACTCTGCCA----------
Length of 5' end seq. 641
3' end seq. ID VFA717Z
3' end seq. 
>VFA717Z.Seq
----------TTAAAAGCAAATGCTGAAATTTTCTCTGTTCAAGGTAAAGCATTAGACAA
AAGTGCTAATCGTGACACTTTAAGAGTTTTAGTTGTAGGTAACCCAGCCAATACCAACGC
ATTAATTGCCGCCCGTAATGCACCAAATATTGACCCAAAACGTTTCTCTGCCATGACTCG
TTTAGATCACAACAGAGGTTTAGCTCAATTAGCCGATAAAACTGGTTCAGCCGTAACTGA
TATTGAAAAATTCTGCATTTGGGGTAACCACTCTGCCACCCAATACCCAGATATTAACTT
TGGTACCGTCAAAGGTAAATCACTCGTAGATACCATCAACGATCAAAAATGGGTTAAAGA
TAACTTTATTCCAACCGTTCAACAAAGAGGTGCCGCTATTATTGCTGCCCGTGGTTTATC
ATCAGCTGCCTCTGCTGCTTCAGCTGCCATCGATCACATGAGAGATTGGACCTATGGTAC
CAATGGTCAATGGACCTCTATGGCCATTTACTCTGAAGGTGAATATGGTGCCGATAAAGG
TCTCTACTTCTCTTTCCCAGTCATTGTAGATAACAAAGGTAAATACGAAATCGTTAAAGG
TCTTAAACTTGATCAATTCTCTCAAGAACGTTTCGATGCTACTCGTAAAGAATTATTATC
AGAAAGGATGGTGTTAAAGAAT
Length of 3' end seq. 672
Connected seq. ID VFA717P
Connected seq. 
>VFA717P.Seq
TACAAATAAAATGAATAAAATTTTAACTCGTAGTTTTAGTACTGGTGCAAATTTCTTTGC
CTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCATTACTGGTGCCAGTGGTCAAATTGG
TTATCAATTATTATTCAGAATTGCCAGTGGTGATATGTTAGGTAAAGATCAACCAATCAT
TCTCCAATGTTTAGAATTACCAGGTGCAATGAACTCATTAAAAGGTGTTTCAATGGAATT
GGATGATTGTGCATTCCCATTGCTCAAAGGTATTGTTCAAAGTGATAAACCAGAGGAAGC
ATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGCTAGTCCAAGAAGCAAGGGTATGGA
GCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTTCTCTGTTCAAGGTAAAGCATTAGA
CAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGTTGTAGGTAACCCAGCCAATACCAA
CGCATTAATTGCCGCCCGTAATGCACCAAATATTGACCCAAAACGTTTCTCTGCCATGAC
TCGTTTAGATCACAACAGAGGTTTAGCTCAATTAGCCGATAAAACTGGTTCAGCCGTAAC
TGATATTGAAAAATTCTGCATTTGGGGTAACCACTCTGCCA----------TTAAAAGCA
AATGCTGAAATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACT
TTAAGAGTTTTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAAT
GCACCAAATATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACAACAGAGGT
TTAGCTCAATTAGCCGATAAAACTGGTTCAGCCGTAACTGATATTGAAAAATTCTGCATT
TGGGGTAACCACTCTGCCACCCAATACCCAGATATTAACTTTGGTACCGTCAAAGGTAAA
TCACTCGTAGATACCATCAACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTT
CAACAAAGAGGTGCCGCTATTATTGCTGCCCGTGGTTTATCATCAGCTGCCTCTGCTGCT
TCAGCTGCCATCGATCACATGAGAGATTGGACCTATGGTACCAATGGTCAATGGACCTCT
ATGGCCATTTACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCA
GTCATTGTAGATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTTGATCAATTC
TCTCAAGAACGTTTCGATGCTACTCGTAAAGAATTATTATCAGAAAGGATGGTGTTAAAG
AAT
Length of connected seq. 1313
Full length Seq ID VFA717E
Full length Seq. 
>VFA717E.Seq
TACAAATAAAATGAATAAAATTTTAACTCGTAGTTTTAGTACTGGTGCAAATTTCTTTGC
CTCTGGTAAAGGTAAAGCACCAGTTAGAGTTGCCATTACTGGTGCCAGTGGTCAAATTGG
TTATCAATTATTATTCAGAATTGCCAGTGGTGATATGTTAGGTAAAGATCAACCAATCAT
TCTCCAATGTTTAGAATTACCAGGTGCAATGAACTCATTAAAAGGTGTTTCAATGGAATT
GGATGATTGTGCATTCCCATTGCTCAAAGGTATTGTTCAAAGTGATAAACCAGAGGAAGC
ATTCGCTGGTGCAGACTATGCATTATTAGTTGGTGCTAGTCCAAGAAGCAAGGGTATGGA
GCGTGGTGACCTTTTAAAAGCAAATGCTGAAATTTTCTCTGTTCAAGGTAAAGCATTAGA
CAAAAGTGCTAATCGTGACACTTTAAGAGTTTTAGTTGTAGGTAACCCAGCCAATACCAA
CGCATTAATTGCCGCCCGTAATGCACCAAATATTGACCCAAAACGTTTCTCTGCCATGAC
TCGTTTAGATCACAACAGAGGTTTAGCTCAATTAGCCGATAAAACTGGTTCAGCCGTAAC
TGATATTGAAAAATTCTGCATTTGGGGTAACCACTCTGCCACCCAATACCCAGATATTAA
CTTTGGTACCGTCAAAGGTAAATCACTCGTAGATACCATCAACGATCAAAAATGGGTTAA
AGATAACTTTATTCCAACCGTTCAACAAAGAGGTGCCGCTATTATTGCTGCCCGTGGTTT
ATCATCAGCTGCCTCTGCTGCTTCAGCTGCCATCGATCACATGAGAGATTGGACCTATGG
TACCAATGGTCAATGGACCTCTATGGCCATTTACTCTGAAGGTGAATATGGTGCCGATAA
AGGTCTCTACTTCTCTTTCCCAGTCATTGTAGATAACAAAGGTAAATACGAAATCGTTAA
AGGTCTTAAACTTGATCAATTCTCTCAAGAACGTTTCGATGCTACTCGTAAAGAATTATT
ATCAGAAAGGATGGTGTTAAAGAAT
Length of full length seq. 1045