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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA620
Library VF
(Link to library)
Clone ID VFA620
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15649-1|Contig-U16150-1
Original site URL
Representative seq. ID VFA620P
(Link to Original site)
Representative DNA sequence 
>VFA620 (VFA620Q) /CSM/VF/VFA6-A/VFA620Q.Seq.d/
AATGTCTAAAGTTNATTACCAAATACTAAATTATTTATTAATAATGAATGGGTAGAGTCA
GTTTCTGGAAAGAAATNAAAACTTTTAATCCAACAAATGGAAAATTAATTTGTGAAGTTT
CAGAAGGTGGTAAAGAAGATGTTGATGTTGCAGTTAAAGCAGCAAGAAATGCATTAGACA
ATGGACCATGGGGTAAAATGACAGCAGAAGATAGAGGTAAATTAATATTGAAATTGGCAG
ATTTAGTTGATCAACATAGAGAGCATTTATCAGATTTAGAAACATTGGATAATGGTAAAC
CATTGACAGCATCAAGTGGTTTTGATATTACTGAAGCTGCTAGAGCTTTACGTTATTTCG
GTGGCTGGGCTGATAAAATTCAAGGTAAAACCATCCCAATTAGTAGTGAATTCACTGCAA
TGACCAAACATGAACCAATTGGTGTAGTTGGATTAATCGTTGCTTGGAATTTCCCATTGT
TACTATTGTCTTGGAAATTGGGACCATCCTTAGCTGCAGGTTGTACAATAGTTGCTAAAC
CATCTGAGTTCACCCCATTGACTGCTTTATACTTTTGTGAATTGGTTAAGGAGGCTGGTT
TCCCACCTGGTGTXXXXXXXXXXTGACTGCGACATTGAATATGTGGCCAATGCTGCAAAA
AATTACGTTTTTGCAAATAGTATGCAGCTATGCTGTGCCGCTTCAAGATTCTTTGTTCAT
GAGAGTATTCTTGATGCTTTCTTGACAATATTCACAGAGAAGATTAAACAATTAAAAGTT
GGTGATCCATATGATAGTGAAACTCATTTAGGTCCATTGGTTAGTAAACAACAACATGAT
CGTGTTTTAGGTTACATTCAAAAGGGTAAAGAGGAAGGTGCAACTTGTCATTTAGGTGGT
GAAGTTCATAATCATCATGCCGATGGTGCTGGTTATTTCATTCAGCCAACTATTTTCACC
AATGTCACTGATGATATGACAATTTGTAAAGAAGAAATCTTTGGTCCTGTTGTTGTAATT
TTACCATTCAAAACTGTTGATGAAGTAATTAAAAGAGCAAATGACACAACCTATGGTTTA
GCAGCTGGTGTTTGGACTAAAGATATTAGTTTAGCATTAAATGTTTCAAATAAATTAAAG
AGTGGTTCAGTTTGGGTAAATGGTTTCAATATCTTAAAATCTCAAATTCCATTTGGTGGT
TTCAAACAATCCGGTTTTGGTCGTATTTATCGAATATGCAATTCAAGGTATTTATCTGTT
AAAGCTGTTACAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
clkxitky*iiy***MGRVSFWKEXKTFNPTNGKLICEVSEGGKEDVDVAVKAARNALDN
GPWGKMTAEDRGKLILKLADLVDQHREHLSDLETLDNGKPLTASSGFDITEAARALRYFG
GWADKIQGKTIPISSEFTAMTKHEPIGVVGLIVAWNFPLLLLSWKLGPSLAAGCTIVAKP
SEFTPLTALYFCELVKEAGFPPG---

---DCDIEYVANAAKNYVFANSMQLCCAASRFFVHESILDAFLTIFTEKIKQLKVGDPYD
SETHLGPLVSKQQHDRVLGYIQKGKEEGATCHLGGEVHNHHADGAGYFIQPTIFTNVTDD
MTICKEEIFGPVVVILPFKTVDEVIKRANDTTYGLAAGVWTKDISLALNVSNKLKSGSVW
VNGFNILKSQIPFGGFKQSGFGRIYRICNSRYLSVKAVT
Translated Amino Acid sequence (All Frames) 
Frame A:
nv*sxlpntklfinnewvesvsgkkxklliqqmen*fvkfqkvvkkmlmlqlkqqemh*t
mdhgvk*qqkievn*y*nwqi*liniesiyqi*khwimvnh*qhqvvlillkllelyvis
vaglikfkvkpsqlvvnslq*pnmnqlv*ld*sllgishcyyclgnwdhp*lqvvq*lln
hlssph*llytfvnwlrrlvshlv---

---*lrh*icgqcckklrfck*yaamlcrfkilcs*eys*cfldnihred*tiksw*si*
**nsfrsig**ttt*scfrlhskg*rgrcnlsfrw*ss*sscrwcwlfhsanyfhqch**
ydnl*rrnlwscccnftiqnc**sn*ksk*hnlwfsswcld*ry*fsikcfk*ikewfsl
gkwfqylkisnsiwwfqtirfwsylsnmqfkvfic*scyn

Frame B:
mskvxyqilnyllimng*sqflernxnf*snkwkinl*sfrrw*rrc*ccs*sskkcirq
wtmg*ndsrr*r*inieigrfs*st*rafirfrnig*w*tidsikwf*yy*sc*sftlfr
wlg**nsr*nhpn***ihcndqt*tnwcswinrclefpivtivleigtilscrlynsc*t
i*vhpidcfill*ig*ggwfptwc---

---DCDIEYVANAAKNYVFANSMQLCCAASRFFVHESILDAFLTIFTEKIKQLKVGDPYD
SETHLGPLVSKQQHDRVLGYIQKGKEEGATCHLGGEVHNHHADGAGYFIQPTIFTNVTDD
MTICKEEIFGPVVVILPFKTVDEVIKRANDTTYGLAAGVWTKDISLALNVSNKLKSGSVW
VNGFNILKSQIPFGGFKQSGFGRIYRICNSRYLSVKAVT

Frame C:
clkxitky*iiy***MGRVSFWKEXKTFNPTNGKLICEVSEGGKEDVDVAVKAARNALDN
GPWGKMTAEDRGKLILKLADLVDQHREHLSDLETLDNGKPLTASSGFDITEAARALRYFG
GWADKIQGKTIPISSEFTAMTKHEPIGVVGLIVAWNFPLLLLSWKLGPSLAAGCTIVAKP
SEFTPLTALYFCELVKEAGFPPG---

---tatlnmwpmlqkitflqivcsyavplqdslfmrvflmls*qysqrrlnn*klvihmi
vkli*vhwlvnnnmivf*vtfkrvkrkvqlvi*vvkfiiimpmvlvisfsqlfspmslmi
*qfvkkkslvlll*fyhskllmk*lkeqmtqpmv*qlvfglkilv*h*mfqin*rvvqfg
*mvsis*nlkfhlvvsnnpvlvvfieyaiqgiyllkllq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA620 (VFA620Q) /CSM/VF/VFA6-A/VFA620Q.Seq.d/                       2454   0.0
VFA436 (VFA436Q) /CSM/VF/VFA4-B/VFA436Q.Seq.d/                       1263   0.0
VFL233 (VFL233Q) /CSM/VF/VFL2-B/VFL233Q.Seq.d/                       1249   0.0
VFK884 (VFK884Q) /CSM/VF/VFK8-D/VFK884Q.Seq.d/                       1249   0.0
VFG787 (VFG787Q) /CSM/VF/VFG7-D/VFG787Q.Seq.d/                       1249   0.0
VFK788 (VFK788Q) /CSM/VF/VFK7-D/VFK788Q.Seq.d/                       1235   0.0
AFK805 (AFK805Q) /CSM/AF/AFK8-A/AFK805Q.Seq.d/                       1233   0.0
CFJ654 (CFJ654Q) /CSM/CF/CFJ6-C/CFJ654Q.Seq.d/                       1227   0.0
VFL820 (VFL820Q) /CSM/VF/VFL8-A/VFL820Q.Seq.d/                       1207   0.0
VFL185 (VFL185Q) /CSM/VF/VFL1-D/VFL185Q.Seq.d/                       1207   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U70317|U70317.1 Dictyostelium discoideum HydA (hydA) gene, partial cds.   422  e-176  7
BX554263|BX554263.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse14d04_q1c.    54  2e-10  4
BX559412|BX559412.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse43d11_q1c.    54  2e-10  4
BP189858|BP189858.1 Dugesia japonica cDNA, clone: 02753_HH, expressed in the head.    48  2e-09  4
AY227203|AY227203.1 Lotus corniculatus aldehyde dehydrogenase 1 precursor (Aldh1) mRNA, complete cds; nuclear gene for mitochondrial product.    38  2e-07  4
BX559491|BX559491.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse43h10_q1c.    54  2e-05  3
AW569561|AW569561.1 si88f05.y1 Gm-c1031 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1031-1786 5' similar to TR:Q43274 Q43274 T CYTOPLASM MALE STERILITY RESTORER FACTOR 2 ;, mRNA sequence.    36  8e-05  4
BG790637|BG790637.1 sae74h11.y1 Gm-c1064 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1064-4198 5' similar to TR:Q43274 Q43274 T CYTOPLASM MALE STERILITY RESTORER FACTOR 2 ;, mRNA sequence.    44  3e-04  3
BE556540|BE556540.1 sq05d03.y1 Unknown Library Type Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1045-2238 5' similar to SW:DHAM_HORSE P12762 ALDEHYDE DEHYDROGENASE, MITOCHONDRIAL ;, mRNA sequence.    36  6e-04  4
AL396242|AL396242.1 T3 end of clone AR0AA029B04 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    40  7e-04  3
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U70317_1(U70317|pid:none) Dictyostelium discoideum HydA (hydA) g...   335   3e-90
DQ767721_1(DQ767721|pid:none) Beauveria bassiana putative aldehy...   216   1e-54
AE017194_2836(AE017194|pid:none) Bacillus cereus ATCC 10987, com...   213   1e-53
AM270171_29(AM270171|pid:none) Aspergillus niger contig An08c016...   213   2e-53
CP000903_2567(CP000903|pid:none) Bacillus weihenstephanensis KBA...   212   3e-53
CP001283_2747(CP001283|pid:none) Bacillus cereus AH820, complete...   209   1e-52
(O14293) RecName: Full=Putative aldehyde dehydrogenase-like prot...   209   2e-52
CP001407_2748(CP001407|pid:none) Bacillus cereus 03BB102, comple...   208   4e-52
CP000485_2405(CP000485|pid:none) Bacillus thuringiensis str. Al ...   208   4e-52
CP001291_3666(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...   207   8e-52
protein update 2009. 6.16
PSORT 

 psg: 0.64  gvh: 0.28  alm: 0.25  top: 0.70  tms: 0.07  mit: 0.28  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFA620 is cyt
5' end seq. ID VFA620F
5' end seq. 
>VFA620F.Seq
AATGTCTAAAGTTNATTACCAAATACTAAATTATTTATTAATAATGAATGGGTAGAGTCA
GTTTCTGGAAAGAAATNAAAACTTTTAATCCAACAAATGGAAAATTAATTTGTGAAGTTT
CAGAAGGTGGTAAAGAAGATGTTGATGTTGCAGTTAAAGCAGCAAGAAATGCATTAGACA
ATGGACCATGGGGTAAAATGACAGCAGAAGATAGAGGTAAATTAATATTGAAATTGGCAG
ATTTAGTTGATCAACATAGAGAGCATTTATCAGATTTAGAAACATTGGATAATGGTAAAC
CATTGACAGCATCAAGTGGTTTTGATATTACTGAAGCTGCTAGAGCTTTACGTTATTTCG
GTGGCTGGGCTGATAAAATTCAAGGTAAAACCATCCCAATTAGTAGTGAATTCACTGCAA
TGACCAAACATGAACCAATTGGTGTAGTTGGATTAATCGTTGCTTGGAATTTCCCATTGT
TACTATTGTCTTGGAAATTGGGACCATCCTTAGCTGCAGGTTGTACAATAGTTGCTAAAC
CATCTGAGTTCACCCCATTGACTGCTTTATACTTTTGTGAATTGGTTAAGGAGGCTGGTT
TCCCACCTGGTGT----------
Length of 5' end seq. 613
3' end seq. ID VFA620Z
3' end seq. 
>VFA620Z.Seq
----------TGACTGCGACATTGAATATGTGGCCAATGCTGCAAAAAATTACGTTTTTG
CAAATAGTATGCAGCTATGCTGTGCCGCTTCAAGATTCTTTGTTCATGAGAGTATTCTTG
ATGCTTTCTTGACAATATTCACAGAGAAGATTAAACAATTAAAAGTTGGTGATCCATATG
ATAGTGAAACTCATTTAGGTCCATTGGTTAGTAAACAACAACATGATCGTGTTTTAGGTT
ACATTCAAAAGGGTAAAGAGGAAGGTGCAACTTGTCATTTAGGTGGTGAAGTTCATAATC
ATCATGCCGATGGTGCTGGTTATTTCATTCAGCCAACTATTTTCACCAATGTCACTGATG
ATATGACAATTTGTAAAGAAGAAATCTTTGGTCCTGTTGTTGTAATTTTACCATTCAAAA
CTGTTGATGAAGTAATTAAAAGAGCAAATGACACAACCTATGGTTTAGCAGCTGGTGTTT
GGACTAAAGATATTAGTTTAGCATTAAATGTTTCAAATAAATTAAAGAGTGGTTCAGTTT
GGGTAAATGGTTTCAATATCTTAAAATCTCAAATTCCATTTGGTGGTTTCAAACAATCCG
GTTTTGGTCGTATTTATCGAATATGCAATTCAAGGTATTTATCTGTTAAAGCTGTTACAA
T
Length of 3' end seq. 651
Connected seq. ID VFA620P
Connected seq. 
>VFA620P.Seq
AATGTCTAAAGTTNATTACCAAATACTAAATTATTTATTAATAATGAATGGGTAGAGTCA
GTTTCTGGAAAGAAATNAAAACTTTTAATCCAACAAATGGAAAATTAATTTGTGAAGTTT
CAGAAGGTGGTAAAGAAGATGTTGATGTTGCAGTTAAAGCAGCAAGAAATGCATTAGACA
ATGGACCATGGGGTAAAATGACAGCAGAAGATAGAGGTAAATTAATATTGAAATTGGCAG
ATTTAGTTGATCAACATAGAGAGCATTTATCAGATTTAGAAACATTGGATAATGGTAAAC
CATTGACAGCATCAAGTGGTTTTGATATTACTGAAGCTGCTAGAGCTTTACGTTATTTCG
GTGGCTGGGCTGATAAAATTCAAGGTAAAACCATCCCAATTAGTAGTGAATTCACTGCAA
TGACCAAACATGAACCAATTGGTGTAGTTGGATTAATCGTTGCTTGGAATTTCCCATTGT
TACTATTGTCTTGGAAATTGGGACCATCCTTAGCTGCAGGTTGTACAATAGTTGCTAAAC
CATCTGAGTTCACCCCATTGACTGCTTTATACTTTTGTGAATTGGTTAAGGAGGCTGGTT
TCCCACCTGGTGT----------TGACTGCGACATTGAATATGTGGCCAATGCTGCAAAA
AATTACGTTTTTGCAAATAGTATGCAGCTATGCTGTGCCGCTTCAAGATTCTTTGTTCAT
GAGAGTATTCTTGATGCTTTCTTGACAATATTCACAGAGAAGATTAAACAATTAAAAGTT
GGTGATCCATATGATAGTGAAACTCATTTAGGTCCATTGGTTAGTAAACAACAACATGAT
CGTGTTTTAGGTTACATTCAAAAGGGTAAAGAGGAAGGTGCAACTTGTCATTTAGGTGGT
GAAGTTCATAATCATCATGCCGATGGTGCTGGTTATTTCATTCAGCCAACTATTTTCACC
AATGTCACTGATGATATGACAATTTGTAAAGAAGAAATCTTTGGTCCTGTTGTTGTAATT
TTACCATTCAAAACTGTTGATGAAGTAATTAAAAGAGCAAATGACACAACCTATGGTTTA
GCAGCTGGTGTTTGGACTAAAGATATTAGTTTAGCATTAAATGTTTCAAATAAATTAAAG
AGTGGTTCAGTTTGGGTAAATGGTTTCAATATCTTAAAATCTCAAATTCCATTTGGTGGT
TTCAAACAATCCGGTTTTGGTCGTATTTATCGAATATGCAATTCAAGGTATTTATCTGTT
AAAGCTGTTACAAT
Length of connected seq. 1264
Full length Seq ID -
Full length Seq. -
Length of full length seq. -