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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA481
Library VF
(Link to library)
Clone ID VFA481
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16320-1
Original site URL
Representative seq. ID VFA481E
(Link to Original site)
Representative DNA sequence 
>VFA481 (VFA481Q) /CSM/VF/VFA4-D/VFA481Q.Seq.d/
ATTAATTTTATATTGTTCTTTTATTTTTTTTNCTTAATATATATATAATNCAAAATGGCA
AGAAGATATGATCAAAGAACAACAATTTTTTCACCAGAAGGTCGTGTTTATCAAGTAGAA
TATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGGT
ATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAATC
TCTGAAGAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATCC
GATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATAT
CAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTAC
ACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCGT
CATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGCT
ACCTCAATTGGTGGTGAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATAAA
CCAGATATTTCATTAAAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTATG
GATCGTTCAAATATCAACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTGAC
AATGTTGTTTATCATATTTTTACTGCAGCAGAATTAGATGCTTTCATTAAAGAAACTGAT
CTCGAACAAGAAACTGAAGATAATTAAAAAAATAAAATAAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
infilffyffxliyi*XKMARRYDQRTTIFSPEGRVYQVEYAMTAIRHAGATVGILAKDG
IVLAAEKKTTAKLLDSSTSISEEMFKIDEHVVCAVAGITSDANILINYARLSSQRFFYQY
QEPMPVEQLVSQICDTKQGYTQYGGLRPFGVSFLYAGWDRHYGFQLYQSDPSGNFAGWKA
TSIGGENSQVAQSVLRSNYKPDISLKEALQLALKVLTKTMDRSNINSEKLEFSYFTKQGD
NVVYHIFTAAELDAFIKETDLEQETEDN*knkik
Translated Amino Acid sequence (All Frames) 
Frame A:
infilffyffxliyi*XKMARRYDQRTTIFSPEGRVYQVEYAMTAIRHAGATVGILAKDG
IVLAAEKKTTAKLLDSSTSISEEMFKIDEHVVCAVAGITSDANILINYARLSSQRFFYQY
QEPMPVEQLVSQICDTKQGYTQYGGLRPFGVSFLYAGWDRHYGFQLYQSDPSGNFAGWKA
TSIGGENSQVAQSVLRSNYKPDISLKEALQLALKVLTKTMDRSNINSEKLEFSYFTKQGD
NVVYHIFTAAELDAFIKETDLEQETEDN*knkik


Frame B:
lilycsfiffx*yiynxkwqedmikeqqffhqkvvfik*nmq*qqldmqvqqlvf*qkmv
lf*qqrkrpqqnf*ihqhqslkrclrlmnmlfvqslvshpmptf*simqdyhhnvsfini
knqcqsnnwslkfviqnkvtlnmvvyvhlvshsfmlvgivimvsnyinlillvillvgkl
pqlvvkivklhnqf*dqiinqifh*kkpfnwlskf*qklwivqistvkn*nslislnkvt
mlfiifllqqn*mlslkklisnkklkiikkik*


Frame C:
*fyivllffxlniyixqngkki*sknnnfftrrsclssricndsn*tcrcnswyfskrwy
cfssrekdhsktfrfininl*rdv*d**tcclcsrwyhircqhfnqlckiiittfllsis
rtnasrtiglsnl*yktrlhsiwwftsiwcliplcwlgsslwfpiisi*sfw*fcwlesy
lnww*k*ssctisfkikl*tryfikrspsigsqsfnknygsfkyqq*kirillfh*tr*q
cclsyfycsrircfh*rn*srtrn*r*lkk*nk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA481 (VFA481Q) /CSM/VF/VFA4-D/VFA481Q.Seq.d/                       1534   0.0
VFI306 (VFI306Q) /CSM/VF/VFI3-A/VFI306Q.Seq.d/                       1503   0.0
AFH827 (AFH827Q) /CSM/AF/AFH8-B/AFH827Q.Seq.d/                       1503   0.0
VFB422 (VFB422Q) /CSM/VF/VFB4-A/VFB422Q.Seq.d/                       1449   0.0
VFJ709 (VFJ709Q) /CSM/VF/VFJ7-A/VFJ709Q.Seq.d/                       1386   0.0
VFA406 (VFA406Q) /CSM/VF/VFA4-A/VFA406Q.Seq.d/                       1382   0.0
SFG154 (SFG154Q) /CSM/SF/SFG1-C/SFG154Q.Seq.d/                       1332   0.0
AHM432 (AHM432Q) /CSM/AH/AHM4-B/AHM432Q.Seq.d/                         42   0.013
VSI254 (VSI254Q) /CSM/VS/VSI2-C/VSI254Q.Seq.d/                         38   0.21
VSH450 (VSH450Q) /CSM/VS/VSH4-C/VSH450Q.Seq.d/                         38   0.21
own update 2002.12.15
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L22212|L22212.1 Dictyostelium discoideum proteasome DD4 gene, complete cds.  1487  0.0  2
AX536828|AX536828.1 Sequence 429 from Patent WO02064766.          72  1e-21  4
AW472197|AW472197.1 si21b11.y1 Gm-c1029 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1029-1846 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    88  7e-21  3
BF596899|BF596899.1 su92h06.y1 Gm-c1055 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1055-2291 5' similar to TR:O82530 O82530 PROTEASOME ALPHA SUBUNIT. ;, mRNA sequence.    86  3e-20  3
AW734093|AW734093.1 sk86f03.y1 Gm-c1035 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1035-246 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    86  3e-20  3
BE579060|BE579060.1 kq53g06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:Y110A7A.F CE23243 ;, mRNA sequence.    78  4e-20  2
AJ291733|AJ291733.1 Nicotiana tabacum partial mRNA for putative alpha3 proteasome  subunit (a3 gene).    50  8e-20  4
BM954104|BM954104.1 sam70c02.y1 Gm-c1069 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1069-4708 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    86  5e-19  2
BE029492|BE029492.1 kp28h03.y1 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to WP:Y110A7A.F CE23243 ;, mRNA sequence.    78  9e-18  2
AI938326|AI938326.1 sc43b11.y1 Gm-c1014 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1014-1846 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    78  2e-16  2
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ228349_1(DQ228349|pid:none) Solanum tuberosum clone 149D10 pro...   317   3e-85
(P52427) RecName: Full=Proteasome subunit alpha type-4;         ...   315   9e-85
GM694699_1(GM694699|pid:none) Sequence 255 from Patent EP1953235.     315   1e-84
(O81148) RecName: Full=Proteasome subunit alpha type-4;         ...   315   1e-84
(O82530) RecName: Full=Proteasome subunit alpha type-4;         ...   313   3e-84
Y13181_1(Y13181|pid:none) Arabidopsis thaliana mRNA for proteaso...   313   4e-84
GM694693_1(GM694693|pid:none) Sequence 249 from Patent EP1953235.     313   4e-84
EF146021_1(EF146021|pid:none) Populus trichocarpa clone WS0114_P...   313   4e-84
EF081846_1(EF081846|pid:none) Picea sitchensis clone WS0276_M22 ...   301   2e-80
(P0C8Y9) RecName: Full=Proteasome subunit alpha type-4-2;       ...   297   2e-79
protein update 2009. 7. 9
PSORT 

 psg: 0.78  gvh: 0.42  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VFA481 is cyt
5' end seq. ID VFA481F
5' end seq. 
>VFA481F.Seq
ATTAATTTTATATTGTTCTTTTATTTTTTTTNCTTAATATATATATAATNCAAAATGGCA
AGAAGATATGATCAAAGAACAACAATTTTTTCACCAGAAGGTCGTGTTTATCAAGTAGAA
TATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGGT
ATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAATC
TCTGAAGAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATCC
GATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATAT
CAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTAC
ACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCGT
CATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGCT
ACCTCAATTGGTGGTGA----------
Length of 5' end seq. 557
3' end seq. ID VFA481Z
3' end seq. 
>VFA481Z.Seq
----------CCACAGCAAAACTTTTAGATTCATCAACATCAATCTCTGAAGAGATGTTT
AAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATCCGATGCCAACATTTTA
ATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATATCAAGAACCAATGCCA
GTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTACACTCAATATGGTGGT
TTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCGTCATTATGGTTTCCAA
TTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGCTACCTCAATTGGTGGT
GAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATAAACCAGATATTTCATTA
AAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTATGGATCGTTCAAATATC
AACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTGACAATGTTGTTTATCAT
ATTTTTACTGCAGCAGAATTAGATGCTTTCATTAAAGAAACTGATCTCGAACAAGAAACT
GAAGATAATTAAAAAAATAAAATAAAA
Length of 3' end seq. 617
Connected seq. ID VFA481P
Connected seq. 
>VFA481P.Seq
ATTAATTTTATATTGTTCTTTTATTTTTTTTNCTTAATATATATATAATNCAAAATGGCA
AGAAGATATGATCAAAGAACAACAATTTTTTCACCAGAAGGTCGTGTTTATCAAGTAGAA
TATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGGT
ATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAATC
TCTGAAGAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATCC
GATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATAT
CAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTAC
ACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCGT
CATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGCT
ACCTCAATTGGTGGTGA----------CCACAGCAAAACTTTTAGATTCATCAACATCAA
TCTCTGAAGAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACAT
CCGATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAAT
ATCAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTT
ACACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATC
GTCATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAG
CTACCTCAATTGGTGGTGAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATA
AACCAGATATTTCATTAAAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTA
TGGATCGTTCAAATATCAACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTG
ACAATGTTGTTTATCATATTTTTACTGCAGCAGAATTAGATGCTTTCATTAAAGAAACTG
ATCTCGAACAAGAAACTGAAGATAATTAAAAAAATAAAATAAAA
Length of connected seq. 1174
Full length Seq ID VFA481E
Full length Seq. 
>VFA481E.Seq
ATTAATTTTATATTGTTCTTTTATTTTTTTTNCTTAATATATATATAATNCAAAATGGCA
AGAAGATATGATCAAAGAACAACAATTTTTTCACCAGAAGGTCGTGTTTATCAAGTAGAA
TATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGGT
ATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAATC
TCTGAAGAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATCC
GATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATAT
CAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTAC
ACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCGT
CATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGCT
ACCTCAATTGGTGGTGAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATAAA
CCAGATATTTCATTAAAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTATG
GATCGTTCAAATATCAACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTGAC
AATGTTGTTTATCATATTTTTACTGCAGCAGAATTAGATGCTTTCATTAAAGAAACTGAT
CTCGAACAAGAAACTGAAGATAATTAAAAAAATAAAATAAAA
Length of full length seq. 822