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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA458
Library VF
(Link to library)
Clone ID VFA458
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16349-1
Original site URL
Representative seq. ID VFA458P
(Link to Original site)
Representative DNA sequence 
>VFA458 (VFA458Q) /CSM/VF/VFA4-C/VFA458Q.Seq.d/
AAAACACACACTCACACACCCAAACATACAAGCAATATAATATATATATAGAAAAAAATG
AAAGTAATATCAGGCTTATTATTTTTCATTTTAATTAGTTGTTCTTTATTTTTAGTTCAA
GGACAAGTTGATTGTGTCACAAATTCAAGTGATGCTAGTTGTACCAATTTTCAATATCCA
TTGGCAAATATAACAGCAGATATCAATAATTTATGTGGTAGCATGCCTTATATGCCAGTT
TGTACCATTCAACAATCATGTAATCAAGAATCCTCAACAAGTGGTATTTGTGATCCATTC
TCAATTCTTGGTGATAGTTGTCTTCACGATATGCCAGGCATGAGTGGTTGTAATAACTTT
AAGAAATTATGTGCATCCGGTTCAGTCGTTGAACAATGTTCAACCGTTGACTCTGTTACC
GATCTTCCAACTACAATGAAAATGTGGGCAAATATTAAAAGTATTTGTAATGAAATGACA
ATGACAGGTTGTGAAAAATGCACAATATTAAATGCCACTTGTGATGTATTAACAGTTTAT
TCCACTCTTTGTCTTGCAATGCCAGAGATGGGTCAATGTGCCAACTGGACCCAAATGTGT
GCXXXXXXXXXXTATTTGCACAGAGATGGCAATGGATGGTTGTGAGAAATGTTCAGGAAA
TTCACCAACTACCACCTGTGATGTTTTGCCAGTTTACTCCTCCCTTTGTATGGCTATGCC
TGATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCCTCCTCTGGTCAACTCTACAA
CTCTCAAATCACATCAGACTATTGTGTCGCCTCTGTTGCTGACGCCGTTCCAATCATGAG
AATGTACTTTCACACTGGTATCTTGGACTATATCCTCTTTAAATCTTGGGTACCAAGAAC
TGACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTATCTTTTTTGAACT
CGAAAAAACCCTTAGAAGTATTTTAGAAAAAAGATGGACTCCCAACAAAAAAGAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kththtpkhtsniiyi*KKMKVISGLLFFILISCSLFLVQGQVDCVTNSSDASCTNFQYP
LANITADINNLCGSMPYMPVCTIQQSCNQESSTSGICDPFSILGDSCLHDMPGMSGCNNF
KKLCASGSVVEQCSTVDSVTDLPTTMKMWANIKSICNEMTMTGCEKCTILNATCDVLTVY
STLCLAMPEMGQCANWTQMC---

---ICTEMAMDGCEKCSGNSPTTTCDVLPVYSSLCMAMPDMSQCANWTKMCSSSGQLYNS
QITSDYCVASVADAVPIMRMYFHTGILDYILFKSWVPRTDRQFAGSWFAIFFFAIFFELE
KTLRSILEKRWTPNKKD
Translated Amino Acid sequence (All Frames) 
Frame A:
kththtpkhtsniiyi*KKMKVISGLLFFILISCSLFLVQGQVDCVTNSSDASCTNFQYP
LANITADINNLCGSMPYMPVCTIQQSCNQESSTSGICDPFSILGDSCLHDMPGMSGCNNF
KKLCASGSVVEQCSTVDSVTDLPTTMKMWANIKSICNEMTMTGCEKCTILNATCDVLTVY
STLCLAMPEMGQCANWTQMC---

---ylhrdgngwl*emfrkftnyhl*cfaslllplygya*yvtmcqldqdvfllwstlql
snhirllcrlcc*rrsnhenvlshwylglypl*ilgtkn*psicwfmvchlflcylf*tr
knp*kyfrkkmdsqqkr

Frame B:
khtlthpniqai*yiyrkk*k*yqayyfsf*lvvlyf*fkdklivsqiqvmlvvpifnih
wqi*qqisiiyvvaclicqfvpfnnhviknpqqvvfvihsqflvivvfticqa*vvvitl
rnyvhpvqslnnvqpltllpifqlq*kcgqilkvfvmk*q*qvvknaqy*mplvmy*qfi
plfvlqcqrwvnvptgpkcv---

---ICTEMAMDGCEKCSGNSPTTTCDVLPVYSSLCMAMPDMSQCANWTKMCSSSGQLYNS
QITSDYCVASVADAVPIMRMYFHTGILDYILFKSWVPRTDRQFAGSWFAIFFFAIFFELE
KTLRSILEKRWTPNKKD

Frame C:
nthshtqtykqyniyieknesnirliifhfn*lffifssrts*lchkfk*c*lyqfsisi
gkynsryq*fmw*halyaslyhstim*srilnkwyl*silnsw**lssryarhewl**l*
eimcirfsr*tmfnr*lcyrssnynenvgky*kyl**ndndrl*kmhnikchl*cinslf
hslscnardgsmcqldpnvc---

---faqrwqwmvvrnvqeihqlppvmfcqftppfvwlclichnvpigprcvpplvnsttl
kshqtivspllltpfqs*ectftlvswtisslnlgyqeltvnllvhglpsfsllsflnsk
kplevf*kkdglptkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA458 (VFA458Q) /CSM/VF/VFA4-C/VFA458Q.Seq.d/                       1911   0.0
VFN811 (VFN811Q) /CSM/VF/VFN8-A/VFN811Q.Seq.d/                       1142   0.0
AFG801 (AFG801Q) /CSM/AF/AFG8-A/AFG801Q.Seq.d/                       1138   0.0
AFC381 (AFC381Q) /CSM/AF/AFC3-D/AFC381Q.Seq.d/                       1138   0.0
AFB137 (AFB137Q) /CSM/AF/AFB1-B/AFB137Q.Seq.d/                       1130   0.0
AFC133 (AFC133Q) /CSM/AF/AFC1-B/AFC133Q.Seq.d/                       1126   0.0
SFA746 (SFA746Q) /CSM/SF/SFA7-B/SFA746Q.Seq.d/                       1094   0.0
VFG652 (VFG652Q) /CSM/VF/VFG6-C/VFG652Q.Seq.d/                       1078   0.0
SFF864 (SFF864Q) /CSM/SF/SFF8-C/SFF864Q.Seq.d/                       1078   0.0
AFJ476 (AFJ476Q) /CSM/AF/AFJ4-D/AFJ476Q.Seq.d/                       1078   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ315489|AJ315489.1 Dictyostelium discoideum mRNA for p80 protein.  1078  0.0  3
AC128362|AC128362.3 Rattus norvegicus clone CH230-259D14, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    54  0.003  1
AC115857|AC115857.5 Mus musculus clone RP24-280I13, WORKING DRAFT SEQUENCE, 7 unordered pieces.    46  0.048  4
AC126186|AC126186.4 Rattus norvegicus clone CH230-467A3, WORKING DRAFT SEQUENCE, 1 ordered piece.    46  0.053  4
AC140767|AC140767.2 Rattus norvegicus clone CH230-118M23, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    50  0.055  1
AC011176|AC011176.3 Mus musculus chromosome 5 clone RP23-3L21 map 5, WORKING DRAFT SEQUENCE, 29 unordered pieces.    40  0.13  4
AC095282|AC095282.4 Rattus norvegicus clone CH230-10J18, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    42  0.13  5
AC133760|AC133760.3 Rattus norvegicus clone CH230-5I7, WORKING DRAFT SEQUENCE, 5 unordered pieces.    54  0.17  3
AF027868|AF027868.1 Bacillus subtilis chromosome region between terC and odhAB.    48  0.22  1
Z99114|Z99114.1 Bacillus subtilis complete genome (section 11 of 21): from 2000171 to 2207900.    48  0.22  1
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7YXD4) RecName: Full=Protein P80; AltName: Full=Endosomal memb...   350   6e-95
DQ646486_1(DQ646486|pid:none) Chlamydomonas reinhardtii transmem...   129   1e-28
AY142139_1(AY142139|pid:none) Giardia intestinalis isolate WB cl...    38   0.49
AY007596_1(AY007596|pid:none) Giardia intestinalis variant-speci...    38   0.49
AF189719_1(AF189719|pid:none) Giardia intestinalis variant-speci...    36   1.9
AJ457171_1(AJ457171|pid:none) Giardia duodenalis partial mRNA fo...    34   9.2
protein update 2009. 2. 4
PSORT 

 psg: 0.99  gvh: 0.85  alm: 0.31  top: 0.13  tms: 0.07  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	  4.0 %: mitochondrial

>> prediction for VFA458 is exc
5' end seq. ID VFA458F
5' end seq. 
>VFA458F.Seq
AAAACACACACTCACACACCCAAACATACAAGCAATATAATATATATATAGAAAAAAATG
AAAGTAATATCAGGCTTATTATTTTTCATTTTAATTAGTTGTTCTTTATTTTTAGTTCAA
GGACAAGTTGATTGTGTCACAAATTCAAGTGATGCTAGTTGTACCAATTTTCAATATCCA
TTGGCAAATATAACAGCAGATATCAATAATTTATGTGGTAGCATGCCTTATATGCCAGTT
TGTACCATTCAACAATCATGTAATCAAGAATCCTCAACAAGTGGTATTTGTGATCCATTC
TCAATTCTTGGTGATAGTTGTCTTCACGATATGCCAGGCATGAGTGGTTGTAATAACTTT
AAGAAATTATGTGCATCCGGTTCAGTCGTTGAACAATGTTCAACCGTTGACTCTGTTACC
GATCTTCCAACTACAATGAAAATGTGGGCAAATATTAAAAGTATTTGTAATGAAATGACA
ATGACAGGTTGTGAAAAATGCACAATATTAAATGCCACTTGTGATGTATTAACAGTTTAT
TCCACTCTTTGTCTTGCAATGCCAGAGATGGGTCAATGTGCCAACTGGACCCAAATGTGT
GC----------
Length of 5' end seq. 602
3' end seq. ID VFA458Z
3' end seq. 
>VFA458Z.Seq
----------TATTTGCACAGAGATGGCAATGGATGGTTGTGAGAAATGTTCAGGAAATT
CACCAACTACCACCTGTGATGTTTTGCCAGTTTACTCCTCCCTTTGTATGGCTATGCCTG
ATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCCTCCTCTGGTCAACTCTACAACT
CTCAAATCACATCAGACTATTGTGTCGCCTCTGTTGCTGACGCCGTTCCAATCATGAGAA
TGTACTTTCACACTGGTATCTTGGACTATATCCTCTTTAAATCTTGGGTACCAAGAACTG
ACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTATCTTTTTTGAACTCG
AAAAAACCCTTAGAAGTATTTTAGAAAAAAGATGGACTCCCAACAAAAAAGAT
Length of 3' end seq. 403
Connected seq. ID VFA458P
Connected seq. 
>VFA458P.Seq
AAAACACACACTCACACACCCAAACATACAAGCAATATAATATATATATAGAAAAAAATG
AAAGTAATATCAGGCTTATTATTTTTCATTTTAATTAGTTGTTCTTTATTTTTAGTTCAA
GGACAAGTTGATTGTGTCACAAATTCAAGTGATGCTAGTTGTACCAATTTTCAATATCCA
TTGGCAAATATAACAGCAGATATCAATAATTTATGTGGTAGCATGCCTTATATGCCAGTT
TGTACCATTCAACAATCATGTAATCAAGAATCCTCAACAAGTGGTATTTGTGATCCATTC
TCAATTCTTGGTGATAGTTGTCTTCACGATATGCCAGGCATGAGTGGTTGTAATAACTTT
AAGAAATTATGTGCATCCGGTTCAGTCGTTGAACAATGTTCAACCGTTGACTCTGTTACC
GATCTTCCAACTACAATGAAAATGTGGGCAAATATTAAAAGTATTTGTAATGAAATGACA
ATGACAGGTTGTGAAAAATGCACAATATTAAATGCCACTTGTGATGTATTAACAGTTTAT
TCCACTCTTTGTCTTGCAATGCCAGAGATGGGTCAATGTGCCAACTGGACCCAAATGTGT
GC----------TATTTGCACAGAGATGGCAATGGATGGTTGTGAGAAATGTTCAGGAAA
TTCACCAACTACCACCTGTGATGTTTTGCCAGTTTACTCCTCCCTTTGTATGGCTATGCC
TGATATGTCACAATGTGCCAATTGGACCAAGATGTGTTCCTCCTCTGGTCAACTCTACAA
CTCTCAAATCACATCAGACTATTGTGTCGCCTCTGTTGCTGACGCCGTTCCAATCATGAG
AATGTACTTTCACACTGGTATCTTGGACTATATCCTCTTTAAATCTTGGGTACCAAGAAC
TGACCGTCAATTTGCTGGTTCATGGTTTGCCATCTTTTTCTTTGCTATCTTTTTTGAACT
CGAAAAAACCCTTAGAAGTATTTTAGAAAAAAGATGGACTCCCAACAAAAAAGAT
Length of connected seq. 1005
Full length Seq ID -
Full length Seq. -
Length of full length seq. -