NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA406
Library VF
(Link to library)
Clone ID VFA406
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16320-1
Original site URL
Representative seq. ID VFA406E
(Link to Original site)
Representative DNA sequence 
>VFA406 (VFA406Q) /CSM/VF/VFA4-A/VFA406Q.Seq.d/
ATTAATTTTATATTGTTCTTTTATTTTTTTTCACTTAATATATATATAATNCAAAATGGC
AAGAAGATATGATCAAAGAACAACAATTTTTTAACCAGAAGGTCGTGTTTATCAAGTAGA
ATATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGG
TATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAAT
CTCTGAAAAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATC
CGATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATA
TCAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTA
CACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCG
TCATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGC
TACCTCAATTGGTGGTGAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATAA
ACCAGATATTTCATTAAAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTAT
GGATCGTTCAAATATCAACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTGA
CAATGTTGTTTATCATANTTTTACCGCACGC
sequence update 2001. 6. 9
Translated Amino Acid sequence 
lilycsfiffhliyi*xkmarrydqrttif*PEGRVYQVEYAMTAIRHAGATVGILAKDG
IVLAAEKKTTAKLLDSSTSISEKMFKIDEHVVCAVAGITSDANILINYARLSSQRFFYQY
QEPMPVEQLVSQICDTKQGYTQYGGLRPFGVSFLYAGWDRHYGFQLYQSDPSGNFAGWKA
TSIGGENSQVAQSVLRSNYKPDISLKEALQLALKVLTKTMDRSNINSEKLEFSYFTKQGD
NVVYHXFTAR
Translated Amino Acid sequence (All Frames) 
Frame A:
infilffyffslniyixqngkki*sknnnfltrrsclssricndsn*tcrcnswyfskrw
ycfssrekdhsktfrfininl*kdv*d**tcclcsrwyhircqhfnqlckiiittfllsi
srtnasrtiglsnl*yktrlhsiwwftsiwcliplcwlgsslwfpiisi*sfw*fcwles
ylnww*k*ssctisfkikl*tryfikrspsigsqsfnknygsfkyqq*kirillfh*tr*
qcclsxfyrt


Frame B:
lilycsfiffhliyi*xkmarrydqrttif*PEGRVYQVEYAMTAIRHAGATVGILAKDG
IVLAAEKKTTAKLLDSSTSISEKMFKIDEHVVCAVAGITSDANILINYARLSSQRFFYQY
QEPMPVEQLVSQICDTKQGYTQYGGLRPFGVSFLYAGWDRHYGFQLYQSDPSGNFAGWKA
TSIGGENSQVAQSVLRSNYKPDISLKEALQLALKVLTKTMDRSNINSEKLEFSYFTKQGD
NVVYHXFTAR


Frame C:
*fyivllffft*yiynxkwqedmikeqqffnqkvvfik*nmq*qqldmqvqqlvf*qkmv
lf*qqrkrpqqnf*ihqhqslkrclrlmnmlfvqslvshpmptf*simqdyhhnvsfini
knqcqsnnwslkfviqnkvtlnmvvyvhlvshsfmlvgivimvsnyinlillvillvgkl
pqlvvkivklhnqf*dqiinqifh*kkpfnwlskf*qklwivqistvkn*nslislnkvt
mlfiixlph
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA406 (VFA406Q) /CSM/VF/VFA4-A/VFA406Q.Seq.d/                       1429   0.0
VFI306 (VFI306Q) /CSM/VF/VFI3-A/VFI306Q.Seq.d/                       1394   0.0
AFH827 (AFH827Q) /CSM/AF/AFH8-B/AFH827Q.Seq.d/                       1394   0.0
VFA481 (VFA481Q) /CSM/VF/VFA4-D/VFA481Q.Seq.d/                       1382   0.0
VFJ709 (VFJ709Q) /CSM/VF/VFJ7-A/VFJ709Q.Seq.d/                       1376   0.0
VFB422 (VFB422Q) /CSM/VF/VFB4-A/VFB422Q.Seq.d/                       1376   0.0
SFG154 (SFG154Q) /CSM/SF/SFG1-C/SFG154Q.Seq.d/                       1336   0.0
AHM432 (AHM432Q) /CSM/AH/AHM4-B/AHM432Q.Seq.d/                         42   0.012
VSI254 (VSI254Q) /CSM/VS/VSI2-C/VSI254Q.Seq.d/                         38   0.19
VSD562 (VSD562Q) /CSM/VS/VSD5-C/VSD562Q.Seq.d/                         38   0.19
own update 2002.12.15
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L22212|L22212.1 Dictyostelium discoideum proteasome DD4 gene, complete cds.  1360  0.0  2
AW472197|AW472197.1 si21b11.y1 Gm-c1029 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1029-1846 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    88  5e-21  3
AJ291733|AJ291733.1 Nicotiana tabacum partial mRNA for putative alpha3 proteasome  subunit (a3 gene).    52  1e-20  4
BF596899|BF596899.1 su92h06.y1 Gm-c1055 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1055-2291 5' similar to TR:O82530 O82530 PROTEASOME ALPHA SUBUNIT. ;, mRNA sequence.    86  2e-20  3
AW734093|AW734093.1 sk86f03.y1 Gm-c1035 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1035-246 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    86  3e-20  3
BM954104|BM954104.1 sam70c02.y1 Gm-c1069 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1069-4708 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    86  5e-19  2
BE579060|BE579060.1 kq53g06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:Y110A7A.F CE23243 ;, mRNA sequence.    70  7e-18  2
AI938326|AI938326.1 sc43b11.y1 Gm-c1014 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1014-1846 5' similar to SW:PRC9_SPIOL P52427 PROTEASOME 27 KD SUBUNIT ;, mRNA sequence.    78  1e-16  2
BE029492|BE029492.1 kp28h03.y1 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to WP:Y110A7A.F CE23243 ;, mRNA sequence.    70  2e-15  2
BI502209|BI502209.1 kt86g02.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to WP:CE23243 Y110A7A.14 endopeptidase ;, mRNA sequence.    70  4e-14  3
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ228349_1(DQ228349|pid:none) Solanum tuberosum clone 149D10 pro...   311   1e-83
(O81148) RecName: Full=Proteasome subunit alpha type-4;         ...   311   2e-83
EF146021_1(EF146021|pid:none) Populus trichocarpa clone WS0114_P...   310   2e-83
GM694699_1(GM694699|pid:none) Sequence 255 from Patent EP1953235.     310   4e-83
Y13181_1(Y13181|pid:none) Arabidopsis thaliana mRNA for proteaso...   309   5e-83
GM694693_1(GM694693|pid:none) Sequence 249 from Patent EP1953235.     309   5e-83
(P52427) RecName: Full=Proteasome subunit alpha type-4;         ...   308   9e-83
(O82530) RecName: Full=Proteasome subunit alpha type-4;         ...   307   3e-82
(P0C8Y9) RecName: Full=Proteasome subunit alpha type-4-2;       ...   295   1e-78
(A2Y9X7) RecName: Full=Proteasome subunit alpha type-4-1;       ...   294   2e-78
protein update 2009. 7. 9
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFA406 is cyt
5' end seq. ID VFA406F
5' end seq. 
>VFA406F.Seq
ATTAATTTTATATTGTTCTTTTATTTTTTTTCACTTAATATATATATAATNCAAAATGGC
AAGAAGATATGATCAAAGAACAACAATTTTTTAACCAGAAGGTCGTGTTTATCAAGTAGA
ATATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGG
TATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAAT
CTCTGAAAAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATC
CGATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATA
TCAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTA
CACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCG
TCATTATGGTTT----------
Length of 5' end seq. 492
3' end seq. ID VFA406Z
3' end seq. 
>VFA406Z.Seq
----------TTTTAGATTCATCAACATCAATCTCTGAAAAGATGTTTAAGATTGATGAA
CATGTTGTTTGTGCAGTCGCTGGTATCACATCCGATGCCAACATTTTAATCAATTATGCA
AGATTATCATCACAACGTTTCTTTTATCAATATCAAGAACCAATGCCAGTCGAACAATTG
GTCTCTCAAATTTGTGATACAAANCAAGGTTACACTCAATATGGTGGTTTACGTCCATTT
GGTGTCTCATTCCTTTATGCTGGTTGGGATCGTCATTATGGTTTCCAATTATATCAATCT
GATCCTTCTGGTAATTTTGCTGGTTGGAAAGCTACCTCAATTGGTGGTGAAAATAGTCAA
GTTGCACAATCAGTTTTAAGATCAAATTATAAACCAGATATTTCATTAAAAGAAGCCCTT
CAATTGGCTCTCAAAGTTTTAACAAAAACTATGGATCGTTCAAATATCAACAGTGAAAAA
TTAGAATTCTCTTATTTCACTAAACAAGGTGACAATGTTGTTTATCATANTTTTACCGCA
CGC
Length of 3' end seq. 533
Connected seq. ID VFA406P
Connected seq. 
>VFA406P.Seq
ATTAATTTTATATTGTTCTTTTATTTTTTTTCACTTAATATATATATAATNCAAAATGGC
AAGAAGATATGATCAAAGAACAACAATTTTTTAACCAGAAGGTCGTGTTTATCAAGTAGA
ATATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGG
TATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAAT
CTCTGAAAAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATC
CGATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATA
TCAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTA
CACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCG
TCATTATGGTTT----------TTTTAGATTCATCAACATCAATCTCTGAAAAGATGTTT
AAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATCCGATGCCAACATTTTA
ATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATATCAAGAACCAATGCCA
GTCGAACAATTGGTCTCTCAAATTTGTGATACAAANCAAGGTTACACTCAATATGGTGGT
TTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCGTCATTATGGTTTCCAA
TTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGCTACCTCAATTGGTGGT
GAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATAAACCAGATATTTCATTA
AAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTATGGATCGTTCAAATATC
AACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTGACAATGTTGTTTATCAT
ANTTTTACCGCACGC
Length of connected seq. 1025
Full length Seq ID VFA406E
Full length Seq. 
>VFA406E.Seq
ATTAATTTTATATTGTTCTTTTATTTTTTTTCACTTAATATATATATAATNCAAAATGGC
AAGAAGATATGATCAAAGAACAACAATTTTTTAACCAGAAGGTCGTGTTTATCAAGTAGA
ATATGCAATGACAGCAATTAGACATGCAGGTGCAACAGTTGGTATTTTAGCAAAAGATGG
TATTGTTTTAGCAGCAGAGAAAAAGACCACAGCAAAACTTTTAGATTCATCAACATCAAT
CTCTGAAAAGATGTTTAAGATTGATGAACATGTTGTTTGTGCAGTCGCTGGTATCACATC
CGATGCCAACATTTTAATCAATTATGCAAGATTATCATCACAACGTTTCTTTTATCAATA
TCAAGAACCAATGCCAGTCGAACAATTGGTCTCTCAAATTTGTGATACAAAACAAGGTTA
CACTCAATATGGTGGTTTACGTCCATTTGGTGTCTCATTCCTTTATGCTGGTTGGGATCG
TCATTATGGTTTCCAATTATATCAATCTGATCCTTCTGGTAATTTTGCTGGTTGGAAAGC
TACCTCAATTGGTGGTGAAAATAGTCAAGTTGCACAATCAGTTTTAAGATCAAATTATAA
ACCAGATATTTCATTAAAAGAAGCCCTTCAATTGGCTCTCAAAGTTTTAACAAAAACTAT
GGATCGTTCAAATATCAACAGTGAAAAATTAGAATTCTCTTATTTCACTAAACAAGGTGA
CAATGTTGTTTATCATANTTTTACCGCACGC
Length of full length seq. 751