>VFA387 (VFA387Q) /CSM/VF/VFA3-D/VFA387Q.Seq.d/
AAACCTTTTTNAATNAACAATATTGTTTTACATATAAATAATAATGTTAAAATNAATTTC
ATTATTAAAAAATAATCAAATATTATTAAAAAATATAATTAATAATGGTAGAATTATAAA
TAATGTTGGTGAAAAATTATCATCAAAATCATTATTAAAAATTAATTATTCATCATCAAC
AACTGATAGAACATTTAATATTTTAGATGGTACAATTGATAAGAATTCAGCAGAATATAA
AGATAATTTAATTAATATGAATTCAACATTAAAACAATTAAAAGAAAATATTGAAAAGAT
TAAATTAGGTGGTGGTGAAAAATTAAATCAAAAGAATATTTCACGTGGAAAGTTATTAGT
ACGTGAACGTATTGAAGCATTGATTGATGTTGGATCACCATTTTTAGAGTTTTCTCAATT
GGCAGGTTGGGGAATGTATGGTAAGGAGGAGGTTGCAGCAGGTGGTATCATCACAGGTAT
TGGTAAAATTCATGXXXXXXXXXXCGTAAGGTTATCGCACGTTTAGTCGATGGTAGTAGA
TTCGATGAATTCAAAGAACTCTATGGCACAACTTTAATTTGTGGTTTTGCACGTGTACAT
GGTATGCCAGTTGGTATCATCGCCAACAACGGTATTCTCTTTAGTGAAAGTGCCGTCAAG
GGTGCCCATTTCATTGAACTTTGCAATCAAAGAGGTATCCCCTTAGTCTTCCTTCAAAAC
ATCACTGGTTTCATGGTTGGTAAAACTTATGAATCTAAAGGTATAGCCAAGGATGGCGCT
AAAATGGTCATGGCTGTTGCCACCGCCAAAGTTCCAAAGATTACAATGATCATTGGTGGT
AGTTTTGGTGCTGGTAATTATGGTATGTGTGGTCGTTCCTACAGTCCACGTTTCCTTTAC
ATGTGGCCAAATGCTAAAATCTCTGTTATGGGTGGAGAACAAGCTGCCTCTGTTTTAGCT
CAAATTCAAAAGGATAACATGGCAAAAGAAAATAAACAATGGTCACCAGAAGAAGAAAAT
ACTTTCAAAAAACCAATCTCTGATAAATTCGAAGAAGAAGGTTCAATCTATTACAGTTCA
GCTCGTTGTTGGGATGATGGTGTTATCGATCCACAAGATTCTCGTAAAGTTATCGCTTTA
AGTTTAAGTGCTTGTANAATCAACCAATNATCCACCATC

nlfxxtilfyi*IIMLKXISLLKNNQILLKNIINNGRIINNVGEKLSSKSLLKINYSSST
TDRTFNILDGTIDKNSAEYKDNLINMNSTLKQLKENIEKIKLGGGEKLNQKNISRGKLLV
RERIEALIDVGSPFLEFSQLAGWGMYGKEEVAAGGIITGIGKIH---

---RKVIARLVDGSRFDEFKELYGTTLICGFARVHGMPVGIIANNGILFSESAVKGAHFI
ELCNQRGIPLVFLQNITGFMVGKTYESKGIAKDGAKMVMAVATAKVPKITMIIGGSFGAG
NYGMCGRSYSPRFLYMWPNAKISVMGGEQAASVLAQIQKDNMAKENKQWSPEEENTFKKP
ISDKFEEEGSIYYSSARCWDDGVIDPQDSRKVIALSLSACXINQXSTI

Frame A:
kpfxxnnivlhinnnvkxnfiikk*sniikkyn**w*nyk*cw*kiiikiiikn*lfiin
n**ni*yfrwyn**efsri*r*fn*yefniktikrky*kd*irww*kikskeyftwkvis
t*ty*sid*cwitifrvfsigrlgnvw*gggcsrwyhhryw*ns---

---RKVIARLVDGSRFDEFKELYGTTLICGFARVHGMPVGIIANNGILFSESAVKGAHFI
ELCNQRGIPLVFLQNITGFMVGKTYESKGIAKDGAKMVMAVATAKVPKITMIIGGSFGAG
NYGMCGRSYSPRFLYMWPNAKISVMGGEQAASVLAQIQKDNMAKENKQWSPEEENTFKKP
ISDKFEEEGSIYYSSARCWDDGVIDPQDSRKVIALSLSACXINQXSTI

Frame B:
nlfxxtilfyi*IIMLKXISLLKNNQILLKNIINNGRIINNVGEKLSSKSLLKINYSSST
TDRTFNILDGTIDKNSAEYKDNLINMNSTLKQLKENIEKIKLGGGEKLNQKNISRGKLLV
RERIEALIDVGSPFLEFSQLAGWGMYGKEEVAAGGIITGIGKIH---

---vrlshv*smvvdsmnsknsmaql*fvvlhvymvcqlvsspttvfslvkvpsrvpisl
nfaikevsp*ssfktslvswlvklmnlkv*prmalkwswllpppkfqrlq*slvvvlvlv
imvcvvvptvhvsftcgqmlksllwvenklplf*lkfkritwqkkinnghqkkkilsknq
slinskkkvqsitvqlvvgmmvlsihkilvklsl*v*vlvxstnxpp

Frame C:
tfxnxqycftyk**c*nxfhy*kiikyy*ki*limvel*imlvknyhqnhy*kliihhqq
liehlif*mvqliriqqnikii*li*iqh*nn*kkilkrln*vvvkn*ikrifhvesy*y
vnvlkh*lmldhhf*sflnwqvgecmvrrrlqqvvssqvlvkfm---

---*gyrtfsrw**ir*iqrtlwhnfnlwfctctwyaswyhrqqrysl**kcrqgcpfh*
tlqskryplslpskhhwfhgw*nl*i*rysqgwr*nghgcchrqsskdyndhww*fwcw*
lwyvwsflqstfplhvakc*nlcygwrtsclcfssnskg*hgkrk*tmvtrrrkyfqktn
l**irrrrfnllqfssllg*wcyrstrfs*syrfkfkclxnqpxihh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA387 (VFA387Q) /CSM/VF/VFA3-D/VFA387Q.Seq.d/                       1913   0.0
AFM534 (AFM534Q) /CSM/AF/AFM5-B/AFM534Q.Seq.d/                       1310   0.0
SLE686 (SLE686Q) /CSM/SL/SLE6-D/SLE686Q.Seq.d/                       1287   0.0
VFK423 (VFK423Q) /CSM/VF/VFK4-A/VFK423Q.Seq.d/                       1140   0.0
SSI206 (SSI206Q) /CSM/SS/SSI2-A/SSI206Q.Seq.d/                       1136   0.0
VSE164 (VSE164Q) /CSM/VS/VSE1-C/VSE164Q.Seq.d/                       1057   0.0
VFK325 (VFK325Q) /CSM/VF/VFK3-B/VFK325Q.Seq.d/                       1015   0.0
SLE678 (SLE678Q) /CSM/SL/SLE6-D/SLE678Q.Seq.d/                        981   0.0
FC-BA21 (FC-BA21Q) /CSM/FC/FC-BA/FC-BA21Q.Seq.d/                      948   0.0
SSJ690 (SSJ690Q) /CSM/SS/SSJ6-D/SSJ690Q.Seq.d/                        922   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.  1292  0.0  3
BM528741|BM528741.1 sak68a05.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-8074 5' similar to TR:O49501 O49501 HYPOTHETICAL 68.9 KD PROTEIN. ;, mRNA sequence.    58  9e-15  4
AK112394|AK112394.1 Ciona intestinalis cDNA, clone:ciad047j21, full insert sequence.    44  1e-14  7
AK115583|AK115583.1 Ciona intestinalis cDNA, clone:cilv005f19, full insert sequence.    44  1e-12  6
CB645046|CB645046.1 ---.    56  1e-09  3
AK060716|AK060716.1 Oryza sativa (japonica cultivar-group) cDNA clone:001-031-D11, full insert sequence.    56  2e-09  3
CD222918|CD222918.1 CCC1_25_G11.b1_A007 Callus culture/cell suspension Sorghum bicolor cDNA clone CCC1_25_G11_A007 3', mRNA sequence.    46  2e-09  3
AU213776|AU213776.1 Caenorhabditis elegans cDNA clone:yk805a03 : 3' end, single read.    48  3e-09  3
BJ146480|BJ146480.1 Caenorhabditis elegans cDNA clone:yk1234a06 : 3' end, single read.    48  3e-09  3
AC084620|AC084620.1 Caenorhabditis briggsae cosmid G44L02, complete sequence.    42  8e-09  6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8T2J9) RecName: Full=Methylcrotonoyl-CoA carboxylase beta chai...   415   e-114
AE008334_10(AE008334|pid:none) Agrobacterium tumefaciens str. C5...   328   2e-88
FP312975_32(FP312975|pid:none) uncultured bacterial clone magm95...   320   6e-86
CP000613_374(CP000613|pid:none) Rhodospirillum centenum SW, comp...   318   3e-85
CP000148_3253(CP000148|pid:none) Geobacter metallireducens GS-15...   318   3e-85
BC091016_1(BC091016|pid:none) Xenopus tropicalis methylcrotonoyl...   315   3e-84
CP000089_92(CP000089|pid:none) Dechloromonas aromatica RCB, comp...   315   3e-84
CU633749_120(CU633749|pid:none) Cupriavidus taiwanensis str. LMG...   313   1e-83
CP000449_1730(CP000449|pid:none) Maricaulis maris MCS10, complet...   313   1e-83
CU633749_1505(CU633749|pid:none) Cupriavidus taiwanensis str. LM...   313   1e-83

 psg: 0.56  gvh: 0.29  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFA387 is cyt

>VFA387F.Seq
AAACCTTTTTNAATNAACAATATTGTTTTACATATAAATAATAATGTTAAAATNAATTTC
ATTATTAAAAAATAATCAAATATTATTAAAAAATATAATTAATAATGGTAGAATTATAAA
TAATGTTGGTGAAAAATTATCATCAAAATCATTATTAAAAATTAATTATTCATCATCAAC
AACTGATAGAACATTTAATATTTTAGATGGTACAATTGATAAGAATTCAGCAGAATATAA
AGATAATTTAATTAATATGAATTCAACATTAAAACAATTAAAAGAAAATATTGAAAAGAT
TAAATTAGGTGGTGGTGAAAAATTAAATCAAAAGAATATTTCACGTGGAAAGTTATTAGT
ACGTGAACGTATTGAAGCATTGATTGATGTTGGATCACCATTTTTAGAGTTTTCTCAATT
GGCAGGTTGGGGAATGTATGGTAAGGAGGAGGTTGCAGCAGGTGGTATCATCACAGGTAT
TGGTAAAATTCATG----------

>VFA387Z.Seq
----------CGTAAGGTTATCGCACGTTTAGTCGATGGTAGTAGATTCGATGAATTCAA
AGAACTCTATGGCACAACTTTAATTTGTGGTTTTGCACGTGTACATGGTATGCCAGTTGG
TATCATCGCCAACAACGGTATTCTCTTTAGTGAAAGTGCCGTCAAGGGTGCCCATTTCAT
TGAACTTTGCAATCAAAGAGGTATCCCCTTAGTCTTCCTTCAAAACATCACTGGTTTCAT
GGTTGGTAAAACTTATGAATCTAAAGGTATAGCCAAGGATGGCGCTAAAATGGTCATGGC
TGTTGCCACCGCCAAAGTTCCAAAGATTACAATGATCATTGGTGGTAGTTTTGGTGCTGG
TAATTATGGTATGTGTGGTCGTTCCTACAGTCCACGTTTCCTTTACATGTGGCCAAATGC
TAAAATCTCTGTTATGGGTGGAGAACAAGCTGCCTCTGTTTTAGCTCAAATTCAAAAGGA
TAACATGGCAAAAGAAAATAAACAATGGTCACCAGAAGAAGAAAATACTTTCAAAAAACC
AATCTCTGATAAATTCGAAGAAGAAGGTTCAATCTATTACAGTTCAGCTCGTTGTTGGGA
TGATGGTGTTATCGATCCACAAGATTCTCGTAAAGTTATCGCTTTAAGTTTAAGTGCTTG
TANAATCAACCAATNATCCACCATC

>VFA387P.Seq
AAACCTTTTTNAATNAACAATATTGTTTTACATATAAATAATAATGTTAAAATNAATTTC
ATTATTAAAAAATAATCAAATATTATTAAAAAATATAATTAATAATGGTAGAATTATAAA
TAATGTTGGTGAAAAATTATCATCAAAATCATTATTAAAAATTAATTATTCATCATCAAC
AACTGATAGAACATTTAATATTTTAGATGGTACAATTGATAAGAATTCAGCAGAATATAA
AGATAATTTAATTAATATGAATTCAACATTAAAACAATTAAAAGAAAATATTGAAAAGAT
TAAATTAGGTGGTGGTGAAAAATTAAATCAAAAGAATATTTCACGTGGAAAGTTATTAGT
ACGTGAACGTATTGAAGCATTGATTGATGTTGGATCACCATTTTTAGAGTTTTCTCAATT
GGCAGGTTGGGGAATGTATGGTAAGGAGGAGGTTGCAGCAGGTGGTATCATCACAGGTAT
TGGTAAAATTCATG----------CGTAAGGTTATCGCACGTTTAGTCGATGGTAGTAGA
TTCGATGAATTCAAAGAACTCTATGGCACAACTTTAATTTGTGGTTTTGCACGTGTACAT
GGTATGCCAGTTGGTATCATCGCCAACAACGGTATTCTCTTTAGTGAAAGTGCCGTCAAG
GGTGCCCATTTCATTGAACTTTGCAATCAAAGAGGTATCCCCTTAGTCTTCCTTCAAAAC
ATCACTGGTTTCATGGTTGGTAAAACTTATGAATCTAAAGGTATAGCCAAGGATGGCGCT
AAAATGGTCATGGCTGTTGCCACCGCCAAAGTTCCAAAGATTACAATGATCATTGGTGGT
AGTTTTGGTGCTGGTAATTATGGTATGTGTGGTCGTTCCTACAGTCCACGTTTCCTTTAC
ATGTGGCCAAATGCTAAAATCTCTGTTATGGGTGGAGAACAAGCTGCCTCTGTTTTAGCT
CAAATTCAAAAGGATAACATGGCAAAAGAAAATAAACAATGGTCACCAGAAGAAGAAAAT
ACTTTCAAAAAACCAATCTCTGATAAATTCGAAGAAGAAGGTTCAATCTATTACAGTTCA
GCTCGTTGTTGGGATGATGGTGTTATCGATCCACAAGATTCTCGTAAAGTTATCGCTTTA
AGTTTAAGTGCTTGTANAATCAACCAATNATCCACCATC