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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB425
Library AF
(Link to library)
Clone ID AFB425
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFB425P
(Link to Original site)
Representative DNA sequence 
>AFB425 (AFB425Q) /CSM/AF/AFB4-B/AFB425Q.Seq.d/
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCGTCAATT
GTTAGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTTXXXXXXXXXXATTTTGAAGNTGAGAATGCAANNGTGNAGCCTCATCATCTGCATTA
GAGNAATCATATGAATTACCAGATGGTCAAGTTATTNCNATTGGTAATGAACGTTTCCGT
TGTCCAGAAGCACTTTTCCANCCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAA
ACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAAT
GTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAA
TTAACTGCACTTGCACCATCANCAATGAAAATTAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iikqlkrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSI
VSRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNEL
RVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMD
SGDGVSHTVPIYEGYALPHAI---

---ILKXRMQXXSLIICIRXII*itrwssyxxw**tfplsrstfpxiilgygvcwys*nn
l*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctixneny
Translated Amino Acid sequence (All Frames) 
Frame A:
iikqlkrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSI
VSRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNEL
RVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMD
SGDGVSHTVPIYEGYALPHAI---

---ILKXRMQXXSLIICIRXII*itrwssyxxw**tfplsrstfpxiilgygvcwys*nn
l*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctixneny

Frame B:
llnnskdfnyililnk*tnkkknkkwmvkmfkl*llimvqvcvklvllvmmlhvlfsrql
lvvqdtlvlwlvwvkrihmsvmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnf
vllqrniqfx*lklh*iqkqierk*lksclklstlqqcmlpfklyyhsmplvvplvslwi
qvmvfpilyqftkvmhyhmlf---

---f*x*ecxxxasssalexsyelpdgqvixignerfrcpealfxpsflgmesagihett
ynsimkcdvdirkdlygnvvlsggttmfpgiadrmnkeltalapsxmki

Frame C:
y*ttqkiliif*y*inkqikkkikngw*rcssfsy**wfryv*swfcw**cstccfpvnc
*sskthwcygwygskgficr**ssikkryfnt*vsn*twychqlg*ygknlasyfl**ts
ccsrgtsssxn*ssiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyrygf
r*wcfpyctnlrrlcittcy---

---fexenaxvxphhlh*xnhmnyqmvklxxlvmnvsvvqkhfsxhhswvwsllvfmkqp
iiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhxq*kl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB425 (AFB425Q) /CSM/AF/AFB4-B/AFB425Q.Seq.d/                       1584   0.0
VFI283 (VFI283Q) /CSM/VF/VFI2-D/VFI283Q.Seq.d/                       1025   0.0
CHD733 (CHD733Q) /CSM/CH/CHD7-B/CHD733Q.Seq.d/                       1025   0.0
AFI479 (AFI479Q) /CSM/AF/AFI4-D/AFI479Q.Seq.d/                       1025   0.0
AFG429 (AFG429Q) /CSM/AF/AFG4-B/AFG429Q.Seq.d/                       1025   0.0
AFG422 (AFG422Q) /CSM/AF/AFG4-A/AFG422Q.Seq.d/                       1025   0.0
AFG308 (AFG308Q) /CSM/AF/AFG3-A/AFG308Q.Seq.d/                       1025   0.0
AFG203 (AFG203Q) /CSM/AF/AFG2-A/AFG203Q.Seq.d/                       1025   0.0
AFD269 (AFD269Q) /CSM/AF/AFD2-C/AFD269Q.Seq.d/                       1025   0.0
AFD237 (AFD237Q) /CSM/AF/AFD2-B/AFD237Q.Seq.d/                       1025   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   791  0.0  2
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   769  0.0  5
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   769  0.0  2
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   769  0.0  9
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     730  0.0  2
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.   644  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   700  0.0  2
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        650  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   650  0.0  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   628  0.0  2
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A03000(A03000)actin 3 - fruit fly (Drosophila melanogaster) (fra...   448   e-124
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   361   2e-98
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   361   2e-98
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   360   5e-98
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   359   6e-98
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        355   1e-96
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   353   3e-96
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   353   4e-96
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   352   7e-96
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   352   1e-95
protein update 2009. 3.27
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFB425 is cyt
5' end seq. ID AFB425F
5' end seq. 
>AFB425F.Seq
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCGTCAATT
GTTAGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTT----------
Length of 5' end seq. 604
3' end seq. ID AFB425Z
3' end seq. 
>AFB425Z.Seq
----------ATTTTGAAGNTGAGAATGCAANNGTGNAGCCTCATCATCTGCATTAGAGN
AATCATATGAATTACCAGATGGTCAAGTTATTNCNATTGGTAATGAACGTTTCCGTTGTC
CAGAAGCACTTTTCCANCCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAAACAA
CCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAATGTTG
TATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAATTAA
CTGCACTTGCACCATCANCAATGAAAATTAT
Length of 3' end seq. 321
Connected seq. ID AFB425P
Connected seq. 
>AFB425P.Seq
ATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAA
AAAAAAAAATAAAAAATGGATGGTGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCA
GGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCGTCAATT
GTTAGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTC
GGTGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGT
ATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTT
CGTGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCA
AATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTT
GCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGAT
TCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGTTATGCATTACCACATGCT
ATTT----------ATTTTGAAGNTGAGAATGCAANNGTGNAGCCTCATCATCTGCATTA
GAGNAATCATATGAATTACCAGATGGTCAAGTTATTNCNATTGGTAATGAACGTTTCCGT
TGTCCAGAAGCACTTTTCCANCCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAA
ACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAAT
GTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAA
TTAACTGCACTTGCACCATCANCAATGAAAATTAT
Length of connected seq. 925
Full length Seq ID -
Full length Seq. -
Length of full length seq. -