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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA302
Library VF
(Link to library)
Clone ID VFA302
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12091-1
Original site URL
Representative seq. ID VFA302E
(Link to Original site)
Representative DNA sequence 
>VFA302 (VFA302Q) /CSM/VF/VFA3-A/VFA302Q.Seq.d/
AAAAAACAATTTAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTAC
TTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGC
TTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAAT
TGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGA
TAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTT
CTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGA
ATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAG
TCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGG
ATTTTAATCATGTTAAACCAACAGAATATAANACCAAGATTATTACATATTAGTGGTGAT
AAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGNCTGT
TTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAAGTCCACAA
GAAAAGAATAAAGCCGCTGAANGTTGCACGTGCAATCGATGCAGAAAGAAAAGGTTTACC
AAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGAAACTTTT
AGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTGAAAAAGT
TTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCACGTGGTAA
AATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTAATGAAAT
CTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCAGTCATGC
AACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAGTTTTAGA
AAATGGTACAAATCAATCATTTAAACTTTATTATCTGCTTAATATAAACACAACAACAAC
AACAACACATTCAAAAAATCAATTTGATCATAAA
sequence update 2001. 6. 9
Translated Amino Acid sequence 
knnlkttntkmiknrklditstnvagigtdldkkcrldaasteaqwkgvgqapglkiwri
enfkvvpvpessygkfydgdsyiilhtfkegnslkhdihfflgtfttqdeagtaayktve
lddflggapiqyrqcqsyespsflslfpkyfilsggvesgf*sc*tnri*xqdyyilvvi
rmlkslkyhwqpvh*ivvxvsfwmqv*LFINSMVVNQVHKKRIKPLXVARAIDAERKGLP
KVEVFCETDSDIPAEFWKLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSRGK
INKSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRVLE
NGTNQSFKLYYLLNINTTTTTTHSKNQFDHK
Translated Amino Acid sequence (All Frames) 
Frame A:
kkqfknnkyknd*e*kiryyfn*ccwywnrfr*km*igcsfn*ssmercwsstrsqdlen
*kf*scpssriiiw*il*w**lyhftyf*rr*ft*t*ysflfryfyysr*swhccl*ns*
ir*flrwctnsissmsii*ksklfiiipkifhfirwc*irilimlnqqnixprllhisgd
knakvaevplatsslnsgxcflldagltiyqfngsksspqeknkaaexctcnrcrkkrft
ks*sfl*nrfryss*iletfrwkrcnrc*trncpnqi*ksfiqiirciwft*il*sftw*
nq*iliki*rcfhh*fr**nlyldrfkiitk*kensiqscnsilgkq*tl*iysns*sfr
kwyksii*tllsa*ykhnnnnntfkksi*s*


Frame B:
knnlkttntkmiknrklditstnvagigtdldkkcrldaasteaqwkgvgqapglkiwri
enfkvvpvpessygkfydgdsyiilhtfkegnslkhdihfflgtfttqdeagtaayktve
lddflggapiqyrqcqsyespsflslfpkyfilsggvesgf*sc*tnri*xqdyyilvvi
rmlkslkyhwqpvh*ivvxvsfwmqv*LFINSMVVNQVHKKRIKPLXVARAIDAERKGLP
KVEVFCETDSDIPAEFWKLLGGKGAIAAKHETAPTKSEKVLYKLSDASGSLKFSEVSRGK
INKSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVNNKRCEYTPIVRVLE
NGTNQSFKLYYLLNINTTTTTTHSKNQFDHK


Frame C:
kti*kqqiqk*lrien*illqlmllvleqi*iknvdwmqlqlklngkvlvkhqvsrfgel
kilklsqfqnhhmvnsmmvivisfyillkkvihlnmifisf*vllllkmklalllikqln
*mis*vvhqfnivnvnhmkvqafyhysqnisfyqvvlnqdfnhvkpteyxtkiity*w**
ec*sr*stignqfie*wxlfpfgcrfnylsiqw**ikstrke*sr*xlhvqsmqkekvyq
klkfsvkpipifqlnfgnf*vekvqsllntklpqpnlkkfytnyqmhlvhlnslkfhvvk
sinph*nlkmfssli*vmksilgsvqnhhqmkrkqhsvmqlntw*tinvvnilq*lef*k
mvqinhlnfiicli*tqqqqqhiqkinlii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA302 (VFA302Q) /CSM/VF/VFA3-A/VFA302Q.Seq.d/                       2151   0.0
VFL329 (VFL329Q) /CSM/VF/VFL3-B/VFL329Q.Seq.d/                       2097   0.0
VFL167 (VFL167Q) /CSM/VF/VFL1-C/VFL167Q.Seq.d/                       2097   0.0
VFI790 (VFI790Q) /CSM/VF/VFI7-D/VFI790Q.Seq.d/                       2097   0.0
VFI559 (VFI559Q) /CSM/VF/VFI5-C/VFI559Q.Seq.d/                       2097   0.0
VFG582 (VFG582Q) /CSM/VF/VFG5-D/VFG582Q.Seq.d/                       2097   0.0
VFG124 (VFG124Q) /CSM/VF/VFG1-A/VFG124Q.Seq.d/                       2097   0.0
VFE814 (VFE814Q) /CSM/VF/VFE8-A/VFE814Q.Seq.d/                       2097   0.0
VFE168 (VFE168Q) /CSM/VF/VFE1-C/VFE168Q.Seq.d/                       2097   0.0
VFD453 (VFD453Q) /CSM/VF/VFD4-C/VFD453Q.Seq.d/                       2097   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

J03515|J03515.1 Slime mold (D.discoideum) severin mRNA, complete cds.   896  0.0  5
U66525|U66525.1 Dictyostelium discoideum ORFveg114 mRNA, complete cds.    46  3e-09  3
BP190606|BP190606.1 Dugesia japonica cDNA, clone: 04979_HH, expressed in the head.    56  0.001  1
AC137821|AC137821.13 Medicago truncatula clone mth2-15o11, WORKING DRAFT SEQUENCE, 2 ordered pieces.    42  0.036  4
AP004171|AP004171.1 Mycoplasma penetrans genomic DNA, complete sequence, section 2/5.    36  0.045  9
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          36  0.23  14
AC104159|AC104159.2 Pan troglodytes clone RP43-82O21, WORKING DRAFT SEQUENCE, 1 ordered pieces.    48  0.25  1
AL031643|AL031643.1 Human DNA sequence from clone 769D20 on chromosome Xp21.1-21.3 Contains EST, STS, GSS.    36  0.52  5
AP002028|AP002028.1 Homo sapiens genomic DNA, chromosome 4q22-q24, clone:2043B18.    38  0.64  7
AL445563|AL445563.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 1/3.    34  0.90  10
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U70047_1(U70047|pid:none) Physarum polycephalum actin-binding pr...   267   6e-70
AM920435_1012(AM920435|pid:none) Penicillium chrysogenum Wiscons...   209   1e-52
AJ621806_1(AJ621806|pid:none) Suberites ficus mRNA for gelsolin ...   207   4e-52
AM270354_42(AM270354|pid:none) Aspergillus niger contig An16c002...   202   2e-50
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...   201   3e-50
FN313917_1(FN313917|pid:none) Schistosoma japonicum isolate Anhu...   181   6e-44
S51363(S51363;S49320) actin modulator protein EWAM - earthworm (...   177   5e-43
AK318626_1(AK318626|pid:none) Arabidopsis thaliana AT3G57410 mRN...   177   8e-43
AY093052_1(AY093052|pid:none) Arabidopsis thaliana unknown prote...   177   8e-43
(O81645) RecName: Full=Villin-3;  &AF081203_1(AF081203|pid:none)...   177   8e-43
protein update 2009. 7. 9
PSORT 

 psg: 0.90  gvh: 0.45  alm: 0.50  top: 0.77  tms: 0.00  mit: 0.37  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for VFA302 is mit
5' end seq. ID VFA302F
5' end seq. 
>VFA302F.Seq
AAAAAACAATTTAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTAC
TTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGC
TTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAAT
TGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGA
TAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTT
CTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGA
ATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAG
TCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGG
ATTTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAGTGGTGATAA
GAATGCTAAAGTCGCT----------
Length of 5' end seq. 556
3' end seq. ID VFA302Z
3' end seq. 
>VFA302Z.Seq
----------TGTTGAATCAGGATTTTAATCATGTTAANCCNNCAGAATATAANNCCNAG
ATTATTNCATATTAGTGGTGATAAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAG
TTCATTGAATAGTGGTGNCTGTTTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAA
TGGTAGTAAATCAAGTCCACAAGAAAAGAATAAAGCCGCTGAANGTTGCACGTGCAATCG
ATGCAGAAAGAAAAGGTTTACCAAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTC
CAGCTGAATTTTGGAAACTTTTAGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTG
CCCCAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCATCTGGTTCACTTAAAT
TCTCTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCA
TCATTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAATCATCACCAAATGAAA
AGAAAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACAATAAACGTTGTGAATATA
CTCCAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTTAAACTTTATTATCTGC
TTAATATAAACACAACAACAACAACAACACATTCAAAAAATCAATTTGATCATAAA
Length of 3' end seq. 706
Connected seq. ID VFA302P
Connected seq. 
>VFA302P.Seq
AAAAAACAATTTAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTAC
TTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGC
TTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAAT
TGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGA
TAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTT
CTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGA
ATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAG
TCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGG
ATTTAATCATGTTAAACCAACAGAATATAAACCAAGATTATTACATATTAGTGGTGATAA
GAATGCTAAAGTCGCT----------TGTTGAATCAGGATTTTAATCATGTTAANCCNNC
AGAATATAANNCCNAGATTATTNCATATTAGTGGTGATAAGAATGCTAAAGTCGCTGAAG
TACCATTGGCAACCAGTTCATTGAATAGTGGTGNCTGTTTCCTTTTGGATGCAGGTTTAA
CTATTTATCAATTCAATGGTAGTAAATCAAGTCCACAAGAAAAGAATAAAGCCGCTGAAN
GTTGCACGTGCAATCGATGCAGAAAGAAAAGGTTTACCAAAAGTTGAAGTTTTCTGTGAA
ACCGATTCCGATATTCCAGCTGAATTTTGGAAACTTTTAGGTGGAAAAGGTGCAATCGCT
GCTAAACACGAAACTGCCCCAACCAAATCTGAAAAAGTTTTATACAAATTATCAGATGCA
TCTGGTTCACTTAAATTCTCTGAAGTTTCACGTGGTAAAATCAATAAATCCTCATTAAAA
TCTGAAGATGTTTTCATCATTGATTTAGGTAATGAAATCTATACTTGGATCGGTTCAAAA
TCATCACCAAATGAAAAGAAAACAGCATTCAGTCATGCAACTCAATACTTGGTAAACAAT
AAACGTTGTGAATATACTCCAATAGTTAGAGTTTTAGAAAATGGTACAAATCAATCATTT
AAACTTTATTATCTGCTTAATATAAACACAACAACAACAACAACACATTCAAAAAATCAA
TTTGATCATAAA
Length of connected seq. 1262
Full length Seq ID VFA302E
Full length Seq. 
>VFA302E.Seq
AAAAAACAATTTAAAAACAACAAATACAAAAATGATTAAGAATAGAAAATTAGATATTAC
TTCAACTAATGTTGCTGGTATTGGAACAGATTTAGATAAAAAATGTAGATTGGATGCAGC
TTCAACTGAAGCTCAATGGAAAGGTGTTGGTCAAGCACCAGGTCTCAAGATTTGGAGAAT
TGAAAATTTTAAAGTTGTCCCAGTTCCAGAATCATCATATGGTAAATTCTATGATGGTGA
TAGTTATATCATTTTACATACTTTTAAAGAAGGTAATTCACTTAAACATGATATTCATTT
CTTTTTAGGTACTTTTACTACTCAAGATGAAGCTGGCACTGCTGCTTATAAAACAGTTGA
ATTAGATGATTTCTTAGGTGGTGCACCAATTCAATATCGTCAATGTCAATCATATGAAAG
TCCAAGCTTTTTATCATTATTCCCAAAATATTTCATTTTATCAGGTGGTGTTGAATCAGG
ATTTTAATCATGTTAAACCAACAGAATATAANACCAAGATTATTACATATTAGTGGTGAT
AAGAATGCTAAAGTCGCTGAAGTACCATTGGCAACCAGTTCATTGAATAGTGGTGNCTGT
TTCCTTTTGGATGCAGGTTTAACTATTTATCAATTCAATGGTAGTAAATCAAGTCCACAA
GAAAAGAATAAAGCCGCTGAANGTTGCACGTGCAATCGATGCAGAAAGAAAAGGTTTACC
AAAAGTTGAAGTTTTCTGTGAAACCGATTCCGATATTCCAGCTGAATTTTGGAAACTTTT
AGGTGGAAAAGGTGCAATCGCTGCTAAACACGAAACTGCCCCAACCAAATCTGAAAAAGT
TTTATACAAATTATCAGATGCATCTGGTTCACTTAAATTCTCTGAAGTTTCACGTGGTAA
AATCAATAAATCCTCATTAAAATCTGAAGATGTTTTCATCATTGATTTAGGTAATGAAAT
CTATACTTGGATCGGTTCAAAATCATCACCAAATGAAAAGAAAACAGCATTCAGTCATGC
AACTCAATACTTGGTAAACAATAAACGTTGTGAATATACTCCAATAGTTAGAGTTTTAGA
AAATGGTACAAATCAATCATTTAAACTTTATTATCTGCTTAATATAAACACAACAACAAC
AACAACACATTCAAAAAATCAATTTGATCATAAA
Length of full length seq. 1174