NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA275
Library VF
(Link to library)
Clone ID VFA275
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11363-1
Original site URL
Representative seq. ID VFA275P
(Link to Original site)
Representative DNA sequence 
>VFA275 (VFA275Q) /CSM/VF/VFA2-D/VFA275Q.Seq.d/
TTTNACATAATGCTAAAACGATGGGTGCAATTAATTTACATGAAATCTNAATTGAGAAAT
CATGGAAATTAAAACAATTTGTTATGGCATCATCGATTGCATCATTATTTGGCTCATTCA
ACCAATGTACTTATGTTTCTGCAAATTTAGTATTAGATTCACTATCAAGATATCGTAAAT
CAATTGGTTTACCATCAACGTGCACAAATTGGGGAACAATTAAATCATCAGGTTTCGTAT
CAAGAAATGATTCAGTTTCAAAAATGTTAGATGATCAAGGTTTCATTCCAATTCCAACAA
ATATAATTTTAGGTTCATTAGATTTACAACTTCAAAATTCAAATAAATATTCAAATTTAA
TTGTTAGTAAATTTAACTATGATTCAATTAAGAAATTCGCATCAGAAACATCAACTTTCC
AAAGATTAGATTTCATTTTAAATTCTATCAAGGAAAAAGATAATGATAATAATGGTGGTG
AAAATATTAGTATTTCAGAGAGTATCCAAATGAAATTTTTGAATAAAATTTCAGAACTCT
TATCAATTGAACAATCAAAGATCAXXXXXXXXXXAATATTAATAATTTACCAGGTGGTTA
TATNAAAAGTAAAGTAGTTGTTGAAAATTTATTTAGAGAAGCAGCAAAAAGAGGTATTCC
ATCAATGTTAATTAGACTACCAAATTATATTTTCACATGAAGAAACAGGTATTGGTCATG
ATTCTGATTTCTTCCAACTAATTATTCAATCATGTTATGCAGTTAAAAAGTATCCAAATA
TTGATAAAAACATATTAACATCACCAATTGGTTGGATAGGTTTAAATACAATTAAAATGA
TTGGAAATGAAAAATGTTGGAATAAATCATCTTCAAATCAATTAAATATCTATAGTTTAA
ATAGTGAAGTTTCATCAATTAAATCATCATTCTCATTCTTGGAAAAGCAATATGGATGTG
AAATGGTTGAATATGAACAATGGAAGAATATCATTAATCAATCAAAGATTCTATCATGTA
TAAAGTTCAAAACATTCCATTCATTAGATAGACTCAATGGTGTTTATAACTTGGGTCATG
GATTTGGTGTTTCAAAGAAAACTAAAGAATTATTACAATCAATGAACTCATATGAAGGAG
GAAGGTTAATAAAAAATCATTCAAAATCATATAAACTAGATCTTTAAAATTAANATAATT
CTTNAAAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
XHNAKTMGAINLHEIXIEKSWKLKQFVMASSIASLFGSFNQCTYVSANLVLDSLSRYRKS
IGLPSTCTNWGTIKSSGFVSRNDSVSKMLDDQGFIPIPTNIILGSLDLQLQNSNKYSNLI
VSKFNYDSIKKFASETSTFQRLDFILNSIKEKDNDNNGGENISISESIQMKFLNKISELL
SIEQSKI---

---NINNLPGGYXKSKVVVENLFREAAKRGIPSMLIRLPNYIFT*rnryws*f*flptny
simlcs*kvsky**khinitnwldrfkyn*ndwk*kmle*iifksikyl*fk**sfin*i
iililgkaiwm*ng*i*tmeeyh*sikdsimykvqnipfir*tqwcl*lgswiwcfken*
riitineli*rrkvnkksfkii*trslklx*fxkk
Translated Amino Acid sequence (All Frames) 
Frame A:
fximlkrwvqliymksxlrnhgn*nnllwhhrlhhylahstnvlmflqi*y*ihyqdivn
qlvyhqraqigeqlnhqvsyqemiqfqkc*mikvsfqfqqi*f*vh*iynfkiqiniqi*
llvnltmiqlrnshqkhqlskd*isf*ilsrkkimiimvvkilvfqrvsk*nf*ikfqns
yqlnnqrs---

---NINNLPGGYXKSKVVVENLFREAAKRGIPSMLIRLPNYIFT*rnryws*f*flptny
simlcs*kvsky**khinitnwldrfkyn*ndwk*kmle*iifksikyl*fk**sfin*i
iililgkaiwm*ng*i*tmeeyh*sikdsimykvqnipfir*tqwcl*lgswiwcfken*
riitineli*rrkvnkksfkii*trslklx*fxkk

Frame B:
xt*c*ndgcn*ft*nln*eimeikticygiidciiiwliqpmylcfckfsirftikis*i
nwftinvhklgnn*iirfrikk*fsfknvr*srfhsnsnkynfrfirfttskfk*ifkfn
c**i*l*fn*eirirninfpkirfhfkfyqgkr****ww*ky*yfreypneife*nfrtl
in*tikd---

---iliiyqvvixkvk*llkiylekqqkevfhqc*ldyqiifsheetgighdsdffqlii
qscyavkkypnidkniltspigwiglntikmignekcwnksssnqlniyslnsevssiks
sfsflekqygcemveyeqwkniinqskilscikfktfhsldrlngvynlghgfgvskktk
ellqsmnsyeggrliknhsksykldl*n*xnsxkn

Frame C:
XHNAKTMGAINLHEIXIEKSWKLKQFVMASSIASLFGSFNQCTYVSANLVLDSLSRYRKS
IGLPSTCTNWGTIKSSGFVSRNDSVSKMLDDQGFIPIPTNIILGSLDLQLQNSNKYSNLI
VSKFNYDSIKKFASETSTFQRLDFILNSIKEKDNDNNGGENISISESIQMKFLNKISELL
SIEQSKI---

---y**ftrwlyxk*ssc*kfi*rsskkrysinvn*ttklyfhmkkqvlvmilissn*lf
nhvmqlksiqilikty*hhqlvg*v*iqlk*lemknvginhlqin*isiv*ivkfhqlnh
hshswksnmdvkwlnmnngrislinqrfyhv*sskhsih*idsmvfitwvmdlvfqrklk
nyynq*thmkeeg**kiiqnhin*ifkixiilxk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA275 (VFA275Q) /CSM/VF/VFA2-D/VFA275Q.Seq.d/                       2311   0.0
CHQ193 (CHQ193Q) /CSM/CH/CHQ1-D/CHQ193Q.Seq.d/                       1162   0.0
AHG850 (AHG850Q) /CSM/AH/AHG8-C/AHG850Q.Seq.d/                        856   0.0
CHR327 (CHR327Q) /CSM/CH/CHR3-B/CHR327Q.Seq.d/                        811   0.0
VHA580 (VHA580Q) /CSM/VH/VHA5-D/VHA580Q.Seq.d/                        809   0.0
SFL454 (SFL454Q) /CSM/SF/SFL4-C/SFL454Q.Seq.d/                         54   5e-06
SFE788 (SFE788Q) /CSM/SF/SFE7-D/SFE788Q.Seq.d/                         54   5e-06
CHG376 (CHG376Q) /CSM/CH/CHG3-D/CHG376Q.Seq.d/                         54   5e-06
SLG354 (SLG354Q) /CSM/SL/SLG3-C/SLG354Q.Seq.d/                         50   8e-05
SHK492 (SHK492Q) /CSM/SH/SHK4-D/SHK492Q.Seq.d/                         50   8e-05
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    82  1e-27  13
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    54  5e-12  15
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    50  2e-11  10
AP004171|AP004171.1 Mycoplasma penetrans genomic DNA, complete sequence, section 2/5.    40  1e-04  16
AF019982|AF019982.1 Dictyostelium discoideum PksA (pksA) gene, partial cds.    40  0.003  3
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    36  0.012  15
AE014823|AE014823.1 Plasmodium falciparum 3D7 chromosome 14 section 8 of 13 of the complete sequence.    32  0.026  13
AC109767|AC109767.5 Rattus norvegicus clone CH230-313B4, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    50  0.065  4
AC025897|AC025897.3 Homo sapiens chromosome 13 clone RP11-384P14 map 13, WORKING DRAFT SEQUENCE, 13 unordered pieces.    36  0.076  10
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38  0.077  15
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54TW0) RecName: Full=Probable polyketide synthase 18;         ...   199   2e-49
AC117075_22(AC117075|pid:none) Dictyostelium discoideum chromoso...   196   1e-48
AC117176_58(AC117176|pid:none) Dictyostelium discoideum chromoso...   178   3e-43
(Q54ED6) RecName: Full=Probable polyketide synthase 41;         ...   175   3e-42
AC116982_23(AC116982|pid:none) Dictyostelium discoideum chromoso...   172   2e-41
AC116982_20(AC116982|pid:none) Dictyostelium discoideum chromoso...   171   4e-41
(Q54KU5) RecName: Full=Probable polyketide synthase 24;         ...   168   4e-40
(Q54KU3) RecName: Full=Probable polyketide synthase 25;         ...   162   2e-38
(Q54FN7) RecName: Full=Probable polyketide synthase 33;         ...   160   1e-37
(Q54FC8) RecName: Full=Probable polyketide synthase 39;         ...   159   2e-37
protein update 2009. 6.16
PSORT 

 psg: 0.81  gvh: 0.52  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFA275 is cyt
5' end seq. ID VFA275F
5' end seq. 
>VFA275F.Seq
TTTNACATAATGCTAAAACGATGGGTGCAATTAATTTACATGAAATCTNAATTGAGAAAT
CATGGAAATTAAAACAATTTGTTATGGCATCATCGATTGCATCATTATTTGGCTCATTCA
ACCAATGTACTTATGTTTCTGCAAATTTAGTATTAGATTCACTATCAAGATATCGTAAAT
CAATTGGTTTACCATCAACGTGCACAAATTGGGGAACAATTAAATCATCAGGTTTCGTAT
CAAGAAATGATTCAGTTTCAAAAATGTTAGATGATCAAGGTTTCATTCCAATTCCAACAA
ATATAATTTTAGGTTCATTAGATTTACAACTTCAAAATTCAAATAAATATTCAAATTTAA
TTGTTAGTAAATTTAACTATGATTCAATTAAGAAATTCGCATCAGAAACATCAACTTTCC
AAAGATTAGATTTCATTTTAAATTCTATCAAGGAAAAAGATAATGATAATAATGGTGGTG
AAAATATTAGTATTTCAGAGAGTATCCAAATGAAATTTTTGAATAAAATTTCAGAACTCT
TATCAATTGAACAATCAAAGATCA----------
Length of 5' end seq. 564
3' end seq. ID VFA275Z
3' end seq. 
>VFA275Z.Seq
----------AATATTAATAATTTACCAGGTGGTTATATNAAAAGTAAAGTAGTTGTTGA
AAATTTATTTAGAGAAGCAGCAAAAAGAGGTATTCCATCAATGTTAATTAGACTACCAAA
TTATATTTTCACATGAAGAAACAGGTATTGGTCATGATTCTGATTTCTTCCAACTAATTA
TTCAATCATGTTATGCAGTTAAAAAGTATCCAAATATTGATAAAAACATATTAACATCAC
CAATTGGTTGGATAGGTTTAAATACAATTAAAATGATTGGAAATGAAAAATGTTGGAATA
AATCATCTTCAAATCAATTAAATATCTATAGTTTAAATAGTGAAGTTTCATCAATTAAAT
CATCATTCTCATTCTTGGAAAAGCAATATGGATGTGAAATGGTTGAATATGAACAATGGA
AGAATATCATTAATCAATCAAAGATTCTATCATGTATAAAGTTCAAAACATTCCATTCAT
TAGATAGACTCAATGGTGTTTATAACTTGGGTCATGGATTTGGTGTTTCAAAGAAAACTA
AAGAATTATTACAATCAATGAACTCATATGAAGGAGGAAGGTTAATAAAAAATCATTCAA
AATCATATAAACTAGATCTTTAAAATTAANATAATTCTTNAAAAAAT
Length of 3' end seq. 637
Connected seq. ID VFA275P
Connected seq. 
>VFA275P.Seq
TTTNACATAATGCTAAAACGATGGGTGCAATTAATTTACATGAAATCTNAATTGAGAAAT
CATGGAAATTAAAACAATTTGTTATGGCATCATCGATTGCATCATTATTTGGCTCATTCA
ACCAATGTACTTATGTTTCTGCAAATTTAGTATTAGATTCACTATCAAGATATCGTAAAT
CAATTGGTTTACCATCAACGTGCACAAATTGGGGAACAATTAAATCATCAGGTTTCGTAT
CAAGAAATGATTCAGTTTCAAAAATGTTAGATGATCAAGGTTTCATTCCAATTCCAACAA
ATATAATTTTAGGTTCATTAGATTTACAACTTCAAAATTCAAATAAATATTCAAATTTAA
TTGTTAGTAAATTTAACTATGATTCAATTAAGAAATTCGCATCAGAAACATCAACTTTCC
AAAGATTAGATTTCATTTTAAATTCTATCAAGGAAAAAGATAATGATAATAATGGTGGTG
AAAATATTAGTATTTCAGAGAGTATCCAAATGAAATTTTTGAATAAAATTTCAGAACTCT
TATCAATTGAACAATCAAAGATCA----------AATATTAATAATTTACCAGGTGGTTA
TATNAAAAGTAAAGTAGTTGTTGAAAATTTATTTAGAGAAGCAGCAAAAAGAGGTATTCC
ATCAATGTTAATTAGACTACCAAATTATATTTTCACATGAAGAAACAGGTATTGGTCATG
ATTCTGATTTCTTCCAACTAATTATTCAATCATGTTATGCAGTTAAAAAGTATCCAAATA
TTGATAAAAACATATTAACATCACCAATTGGTTGGATAGGTTTAAATACAATTAAAATGA
TTGGAAATGAAAAATGTTGGAATAAATCATCTTCAAATCAATTAAATATCTATAGTTTAA
ATAGTGAAGTTTCATCAATTAAATCATCATTCTCATTCTTGGAAAAGCAATATGGATGTG
AAATGGTTGAATATGAACAATGGAAGAATATCATTAATCAATCAAAGATTCTATCATGTA
TAAAGTTCAAAACATTCCATTCATTAGATAGACTCAATGGTGTTTATAACTTGGGTCATG
GATTTGGTGTTTCAAAGAAAACTAAAGAATTATTACAATCAATGAACTCATATGAAGGAG
GAAGGTTAATAAAAAATCATTCAAAATCATATAAACTAGATCTTTAAAATTAANATAATT
CTTNAAAAAAT
Length of connected seq. 1201
Full length Seq ID -
Full length Seq. -
Length of full length seq. -