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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA274
Library VF
(Link to library)
Clone ID VFA274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14973-1
Original site URL
Representative seq. ID VFA274P
(Link to Original site)
Representative DNA sequence 
>VFA274 (VFA274Q) /CSM/VF/VFA2-D/VFA274Q.Seq.d/
CACAAACNAAATAAAAATGTTATCAAATTTTTTAAAAGTNACAGTAAAGCACTAGGACAT
ATAAGAACTTTTGCCTCAAAGAGTGGTGAAATAAACACAATTTTAAAAAAGCCGATACAT
ACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAACTCATTTCATTCT
TCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGTTTATACAAAAAAA
AATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAAGAAGCTGTTTGTGCAGGTTTAG
AAAGTGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGATCACACTTATATGT
TATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATGAAAGAAACTGGTT
GTTCAAAGGGTAAAGGTGGTTCAATGCATATGTTTACAAAGAATTTCTATGGTGGTAATG
GTATTGTTGGTGCTCAATGTCCACTTGGTGCTGGTATTGCCTTTGCACAAAAGTACAATA
AGACCGGTAATGTTTGTTTAGCCATGTATGGTGATGGTXXXXXXXXXXATGTTTGTTTAG
CCATGTATGGTGATGGTGCTGCCAATCAAAGGTCAATTATTTGAAGCCTTCAACATGGCT
TCACTTTGGAACCTTCCAGTCATTTTCATTTGTGAAAACAATAAATACGGTATGGGTACC
TCTCAAAANANATCAACTGCTGGTCACGATTTNTATACTCGTGGTCACTATGTTGCAGGC
TTAAAAGTCGATGGTATGGATGTTTTCGCCGTCAAANAAGCTGGTAAATACGCCGCTGAA
TGGTGTAGAGCTGGTAATGGTCCAATCATTTTAGAAATGGATACCTATCGTTATGTAGGT
CACTCTATGTNTGATCCAGGTATCACTTATNGNACAANAGAAGAAGTTAACCACGTTAGA
CAAACTAGAGATCCAATTGAAAACATTCGTCAAATCATCTTGGACAATAAAATCGCAACT
GAAGATCAACTCGCTGCCATCGAAGAAACCGTTAGAGATGAAATGGAAAAAGCCTCTGAA
AAAGCTATCGCTGCTCCATTACCACAAGCTCGTGAACTNTTTACCAACGTTTACCTCCAA
GAAGTTCCAGTAAGAGGTGTNAATTTGTTAACAGTTTCAAACCAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
qtk*kcyqif*kxq*strtyknfclkew*NKHNFKKADTYLCDGPSDSTVTNKDELISFF
TEMSRFRRLETVCDGLYKKKLIRGFCHLYTGQEAVCAGLESAITKDDHIITAYRDHTYML
SRGATPEEIFAELLMKETGCSKGKGGSMHMFTKNFYGGNGIVGAQCPLGAGIAFAQKYNK
TGNVCLAMYGDG---

---VCLAMYGDGAANQRSII*slqhgftlepsshfhl*kq*irygylskxincwsrfxys
wslccrlksrwygcfrrqxsw*irr*mv*sw*wsnhfrngylslcrslyv*sryhlxxxr
rs*pr*tn*rsn*khssnhlgq*nrn*rstrchrrnr*r*ngksl*ksyrcsittss*tx
yqrlpprssskrcxfvnsfkp
Translated Amino Acid sequence (All Frames) 
Frame A:
hkxnknvikffksxskalghirtfasksgeintilkkpihtyvmvhltqllptkmnsfhs
slkcqdledsklfvmvytkkn*levsviytlvkklfvqv*kvqllkmiillqhteitlic
yqeeqhqkkflqny**kklvvqrvkvvqciclqrismvvmvllvlnvhlvlvlplhksti
rpvmfv*pcmvm---

---mfv*pcmvmvlpikgqlfeafnmaslwnlpvificennkygmgtsqxxstaghdxyt
rghyvaglkvdgmdvfavkxagkyaaewcragngpiilemdtyryvghsmxdpgityxtx
eevnhvrqtrdpienirqiildnkiatedqlaaieetvrdemekasekaiaaplpqarel
ftnvylqevpvrgvnlltvsnh

Frame B:
tnxikmlsnflkvtvkh*di*ellpqrvvk*tqf*ksryilm*wsi*lncyqqr*thfil
h*nvki*ktrncl*wfiqkkin*rflsfihwsrsclcrfrkcny*r*syyysiqrshlyv
ikrsntrrnfcriinernwlfkg*rwfnayvykeflww*wycwcsmstwcwyclctkvq*
dr*clfshvw*w---

---clfshvw*wccqskvnylkpstwlhfgtfqsfsfvktintvwvplkxxqllvtixil
vvtmlqa*ksmvwmfspsxklvntplngvelvmvqsf*kwipivm*vtlcxiqvslxxqx
kklttldkleiqlktfvksswtiksqlkinslpskkplemkwkkplkklsllhyhklvnx
lptftskkfq*evxic*qfqt

Frame C:
qtk*kcyqif*kxq*strtyknfclkew*NKHNFKKADTYLCDGPSDSTVTNKDELISFF
TEMSRFRRLETVCDGLYKKKLIRGFCHLYTGQEAVCAGLESAITKDDHIITAYRDHTYML
SRGATPEEIFAELLMKETGCSKGKGGSMHMFTKNFYGGNGIVGAQCPLGAGIAFAQKYNK
TGNVCLAMYGDG---

---VCLAMYGDGAANQRSII*slqhgftlepsshfhl*kq*irygylskxincwsrfxys
wslccrlksrwygcfrrqxsw*irr*mv*sw*wsnhfrngylslcrslyv*sryhlxxxr
rs*pr*tn*rsn*khssnhlgq*nrn*rstrchrrnr*r*ngksl*ksyrcsittss*tx
yqrlpprssskrcxfvnsfkp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA274 (VFA274Q) /CSM/VF/VFA2-D/VFA274Q.Seq.d/                       2165   0.0
AFE319 (AFE319Q) /CSM/AF/AFE3-A/AFE319Q.Seq.d/                       1092   0.0
VFL810 (VFL810Q) /CSM/VF/VFL8-A/VFL810Q.Seq.d/                       1086   0.0
VFL294 (VFL294Q) /CSM/VF/VFL2-D/VFL294Q.Seq.d/                       1080   0.0
SFI357 (SFI357Q) /CSM/SF/SFI3-C/SFI357Q.Seq.d/                       1078   0.0
CFH686 (CFH686Q) /CSM/CF/CFH6-D/CFH686Q.Seq.d/                       1074   0.0
SFI644 (SFI644Q) /CSM/SF/SFI6-B/SFI644Q.Seq.d/                       1072   0.0
SFI495 (SFI495Q) /CSM/SF/SFI4-D/SFI495Q.Seq.d/                       1072   0.0
VFM465 (VFM465Q) /CSM/VF/VFM4-C/VFM465Q.Seq.d/                       1068   0.0
SFC663 (SFC663Q) /CSM/SF/SFC6-C/SFC663Q.Seq.d/                       1068   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

CA868872|CA868872.1 pw91b05.y1 Haemonchus contortus intestine pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  7e-21  5
AF209924|AF209924.1 Lycopersicon esculentum pyruvate dehydrogenase mRNA, partial cds.    52  9e-21  5
BM111840|BM111840.1 EST559376 potato roots Solanum tuberosum cDNA clone cPRO12F13 5' end, mRNA sequence.    52  7e-20  5
CB064381|CB064381.1 py07e03.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_ASCSU P26267 PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT TYPE I, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  1e-18  5
Z26949|Z26949.1 S.tuberosum E1 alpha subunit of pyruvate dehydrogenase (lipoamide).    52  1e-18  5
CB191084|CB191084.1 py39h04.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_ASCSU P26267 PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT TYPE I, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  1e-18  5
CA869913|CA869913.1 px13e02.y1 Haemonchus contortus intestine pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  1e-18  5
CB063951|CB063951.1 py02b02.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  1e-18  5
CB099517|CB099517.1 py13e09.y1 Haemonchus contortus whole worm pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  1e-18  5
CA869182|CA869182.1 pw99b06.y1 Haemonchus contortus intestine pAMP1 v1 Haemonchus contortus cDNA 5' similar to SW:ODPA_CAEEL P52899 PROBABLE PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    60  1e-18  5
dna update 2003. 8.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C70) RecName: Full=Pyruvate dehydrogenase E1 component subun...   343   0.0
EU186336_1(EU186336|pid:none) Brassica rapa clone S66 pyruvate d...   196   e-100
(Q8H1Y0) RecName: Full=Pyruvate dehydrogenase E1 component subun...   194   3e-99
AB090280_1(AB090280|pid:none) Beta vulgaris PDH E1a-2 mRNA for p...   196   6e-98
DQ191640_1(DQ191640|pid:none) Solanum tuberosum unknown mRNA.         192   8e-98
AF209924_1(AF209924|pid:none) Lycopersicon esculentum pyruvate d...   192   1e-97
AC146588_13(AC146588|pid:none) Medicago truncatula clone mth2-14...   189   5e-97
BT038357_1(BT038357|pid:none) Zea mays full-length cDNA clone ZM...   193   9e-97
EU976956_1(EU976956|pid:none) Zea mays clone 994766 pyruvate deh...   193   1e-96
CP000581_566(CP000581|pid:none) Ostreococcus lucimarinus CCE9901...   198   2e-96
protein update 2009. 6.16
PSORT 

 psg: 0.73  gvh: 0.22  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for VFA274 is nuc
5' end seq. ID VFA274F
5' end seq. 
>VFA274F.Seq
CACAAACNAAATAAAAATGTTATCAAATTTTTTAAAAGTNACAGTAAAGCACTAGGACAT
ATAAGAACTTTTGCCTCAAAGAGTGGTGAAATAAACACAATTTTAAAAAAGCCGATACAT
ACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAACTCATTTCATTCT
TCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGTTTATACAAAAAAA
AATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAAGAAGCTGTTTGTGCAGGTTTAG
AAAGTGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGATCACACTTATATGT
TATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATGAAAGAAACTGGTT
GTTCAAAGGGTAAAGGTGGTTCAATGCATATGTTTACAAAGAATTTCTATGGTGGTAATG
GTATTGTTGGTGCTCAATGTCCACTTGGTGCTGGTATTGCCTTTGCACAAAAGTACAATA
AGACCGGTAATGTTTGTTTAGCCATGTATGGTGATGGT----------
Length of 5' end seq. 578
3' end seq. ID VFA274Z
3' end seq. 
>VFA274Z.Seq
----------ATGTTTGTTTAGCCATGTATGGTGATGGTGCTGCCAATCAAAGGTCAATT
ATTTGAAGCCTTCAACATGGCTTCACTTTGGAACCTTCCAGTCATTTTCATTTGTGAAAA
CAATAAATACGGTATGGGTACCTCTCAAAANANATCAACTGCTGGTCACGATTTNTATAC
TCGTGGTCACTATGTTGCAGGCTTAAAAGTCGATGGTATGGATGTTTTCGCCGTCAAANA
AGCTGGTAAATACGCCGCTGAATGGTGTAGAGCTGGTAATGGTCCAATCATTTTAGAAAT
GGATACCTATCGTTATGTAGGTCACTCTATGTNTGATCCAGGTATCACTTATNGNACAAN
AGAAGAAGTTAACCACGTTAGACAAACTAGAGATCCAATTGAAAACATTCGTCAAATCAT
CTTGGACAATAAAATCGCAACTGAAGATCAACTCGCTGCCATCGAAGAAACCGTTAGAGA
TGAAATGGAAAAAGCCTCTGAAAAAGCTATCGCTGCTCCATTACCACAAGCTCGTGAACT
NTTTACCAACGTTTACCTCCAAGAAGTTCCAGTAAGAGGTGTNAATTTGTTAACAGTTTC
AAACCAT
Length of 3' end seq. 597
Connected seq. ID VFA274P
Connected seq. 
>VFA274P.Seq
CACAAACNAAATAAAAATGTTATCAAATTTTTTAAAAGTNACAGTAAAGCACTAGGACAT
ATAAGAACTTTTGCCTCAAAGAGTGGTGAAATAAACACAATTTTAAAAAAGCCGATACAT
ACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAACTCATTTCATTCT
TCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGTTTATACAAAAAAA
AATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAAGAAGCTGTTTGTGCAGGTTTAG
AAAGTGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGATCACACTTATATGT
TATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATGAAAGAAACTGGTT
GTTCAAAGGGTAAAGGTGGTTCAATGCATATGTTTACAAAGAATTTCTATGGTGGTAATG
GTATTGTTGGTGCTCAATGTCCACTTGGTGCTGGTATTGCCTTTGCACAAAAGTACAATA
AGACCGGTAATGTTTGTTTAGCCATGTATGGTGATGGT----------ATGTTTGTTTAG
CCATGTATGGTGATGGTGCTGCCAATCAAAGGTCAATTATTTGAAGCCTTCAACATGGCT
TCACTTTGGAACCTTCCAGTCATTTTCATTTGTGAAAACAATAAATACGGTATGGGTACC
TCTCAAAANANATCAACTGCTGGTCACGATTTNTATACTCGTGGTCACTATGTTGCAGGC
TTAAAAGTCGATGGTATGGATGTTTTCGCCGTCAAANAAGCTGGTAAATACGCCGCTGAA
TGGTGTAGAGCTGGTAATGGTCCAATCATTTTAGAAATGGATACCTATCGTTATGTAGGT
CACTCTATGTNTGATCCAGGTATCACTTATNGNACAANAGAAGAAGTTAACCACGTTAGA
CAAACTAGAGATCCAATTGAAAACATTCGTCAAATCATCTTGGACAATAAAATCGCAACT
GAAGATCAACTCGCTGCCATCGAAGAAACCGTTAGAGATGAAATGGAAAAAGCCTCTGAA
AAAGCTATCGCTGCTCCATTACCACAAGCTCGTGAACTNTTTACCAACGTTTACCTCCAA
GAAGTTCCAGTAAGAGGTGTNAATTTGTTAACAGTTTCAAACCAT
Length of connected seq. 1175
Full length Seq ID -
Full length Seq. -
Length of full length seq. -