>VFA272 (VFA272Q) /CSM/VF/VFA2-C/VFA272Q.Seq.d/
GATNATTTATTTATTTATTNAATATATATTATATTAAATAAAATGTTTGCCAGATTAGCC
AGAGCTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGCCAACCTTAAAAGAA
AGATTAGTTCAAATCATCCCAGGTAAAATTGAACAAGTTAAACAATTAAAAACTGAACAT
GGTGACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTGGTATGAGATCAGTT
AAATCATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCATTAGATTCCGTGGT
TTAACAATCCCAGAATGTCAAGAGAAATTACCAAAAGCTCCAGGTGGTGCTGAACCATTA
CCAGAAGGTATTTTATGGTTACTTTTAACTGGTGAAGTTCCAACTGAATCACAAGTTAAA
ACCTTATCAAAAGATTTAGCTAAACGTGCTGGTTTACCAAAACACGTCACCTCAATGATT
AAAGCATTCCCAGAGCAAATGCATCCAATGTCACAATTGGCCGCTGCTATTTTAGCACTC
CAAGGTGAAAGCAAATTCGTCAAAGCCTACAATGATGGTGTCAAAAAAGATAAATATTGG
GAATCAACTTTAGAAGXXXXXXXXXX

DXLFIYXIYIILNKMFARLARANYLKNSGRYFSTEPTLKERLVQIIPGKIEQVKQLKTEH
GDKIIGTCTVAQAYGGMRSVKSLVTETSSLDPEEGIRFRGLTIPECQEKLPKAPGGAEPL
PEGILWLLLTGEVPTESQVKTLSKDLAKRAGLPKHVTSMIKAFPEQMHPMSQLAAAILAL
QGESKFVKAYNDGVKKDKYWESTLE---

Frame A:
DXLFIYXIYIILNKMFARLARANYLKNSGRYFSTEPTLKERLVQIIPGKIEQVKQLKTEH
GDKIIGTCTVAQAYGGMRSVKSLVTETSSLDPEEGIRFRGLTIPECQEKLPKAPGGAEPL
PEGILWLLLTGEVPTESQVKTLSKDLAKRAGLPKHVTSMIKAFPEQMHPMSQLAAAILAL
QGESKFVKAYNDGVKKDKYWESTLE---


Frame B:
xiylfixyily*ikclpd*peltt*riqvdtsllsqp*kkd*fkssqvklnklnn*klnm
vtkslvpvllhklmvv*dqlnh*llkhhh*iqkkaldsvv*qsqnvkrnyqklqvvlnhy
qkvfygyf*lvkfqlnhklkpyqki*lnvlvyqntspq*lkhsqskciqchnwpllf*hs
kvkansskptmmvskkinignql*---


Frame C:
xfiylxniyyik*nvcqisqs*llkefr*illy*anlkrkissnhpr*n*ts*tikn*tw
*qnhwylycctslwwyeis*iisy*niiirsrrrh*ipwfnnprmsreitkssrwc*tit
rryfmvtfnw*ssn*its*nlikrfs*tcwftktrhlnd*sipranasnvtigrcyfstp
r*kqirqslq*wcqkr*ilginfr---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM856 (VFM856Q) /CSM/VF/VFM8-C/VFM856Q.Seq.d/                       1150   0.0
VFM247 (VFM247Q) /CSM/VF/VFM2-B/VFM247Q.Seq.d/                       1150   0.0
VFK207 (VFK207Q) /CSM/VF/VFK2-A/VFK207Q.Seq.d/                       1150   0.0
VFK145 (VFK145Q) /CSM/VF/VFK1-B/VFK145Q.Seq.d/                       1150   0.0
VFI837 (VFI837Q) /CSM/VF/VFI8-B/VFI837Q.Seq.d/                       1150   0.0
VFI584 (VFI584Q) /CSM/VF/VFI5-D/VFI584Q.Seq.d/                       1150   0.0
VFI101 (VFI101Q) /CSM/VF/VFI1-A/VFI101Q.Seq.d/                       1150   0.0
VFF671 (VFF671Q) /CSM/VF/VFF6-C/VFF671Q.Seq.d/                       1150   0.0
VFE391 (VFE391Q) /CSM/VF/VFE3-D/VFE391Q.Seq.d/                       1150   0.0
VFE288 (VFE288Q) /CSM/VF/VFE2-D/VFE288Q.Seq.d/                       1150   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   793  0.0  4
AY126274|AY126274.1 Candida krusei citrate synthase (CS1) gene, complete cds.    54  1e-10  3
AX536976|AX536976.1 Sequence 577 from Patent WO02064766.          76  6e-10  1
AL429256|AL429256.1 clone BA0AB030F08 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    64  1e-09  2
AY145050|AY145050.1 Kluyveromyces lactis clone P2263 CIT1 gene, partial cds.    64  2e-09  2
CB832670|CB832670.1 USDA-FP_100461 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-07_A07 5', mRNA sequence.    46  2e-05  3
BE581402|BE581402.1 kq45d02.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:T20G5.2 CE00513 PROBABLE CITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    34  2e-05  4
CD526170|CD526170.1 kw31d09.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:CISY_CAEEL P34575 PROBABLE CITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    46  1e-04  3
CD450751|CD450751.1 USDA-FP_102714 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-37_E02 5', mRNA sequence.    46  0.001  2
BE579168|BE579168.1 kq23d01.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:T20G5.2 CE00513 PROBABLE CITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    34  0.002  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553V1) RecName: Full=Citrate synthase, mitochondrial;         ...   381   e-104
AC116305_8(AC116305|pid:none) Dictyostelium discoideum chromosom...   380   e-104
FN392319_422(FN392319|pid:none) Pichia pastoris GS115 chromosome...   221   1e-56
FM992695_315(FM992695|pid:none) Candida dubliniensis CD36 chromo...   219   5e-56
(P79024) RecName: Full=Citrate synthase, mitochondrial;         ...   217   2e-55
CP000502_263(CP000502|pid:none) Pichia stipitis CBS 6054 chromos...   216   5e-55
AY144810_1(AY144810|pid:none) Saccharomyces bayanus clone Contig...   214   1e-54
(P00890) RecName: Full=Citrate synthase, mitochondrial;         ...   213   3e-54
CR382131_112(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   213   5e-54
CU928180_527(CU928180|pid:none) Kluyveromyces thermotolerans str...   213   5e-54

 psg: 0.75  gvh: 0.62  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VFA272 is nuc

>VFA272F.Seq
GATNATTTATTTATTTATTNAATATATATTATATTAAATAAAATGTTTGCCAGATTAGCC
AGAGCTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGCCAACCTTAAAAGAA
AGATTAGTTCAAATCATCCCAGGTAAAATTGAACAAGTTAAACAATTAAAAACTGAACAT
GGTGACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTGGTATGAGATCAGTT
AAATCATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCATTAGATTCCGTGGT
TTAACAATCCCAGAATGTCAAGAGAAATTACCAAAAGCTCCAGGTGGTGCTGAACCATTA
CCAGAAGGTATTTTATGGTTACTTTTAACTGGTGAAGTTCCAACTGAATCACAAGTTAAA
ACCTTATCAAAAGATTTAGCTAAACGTGCTGGTTTACCAAAACACGTCACCTCAATGATT
AAAGCATTCCCAGAGCAAATGCATCCAATGTCACAATTGGCCGCTGCTATTTTAGCACTC
CAAGGTGAAAGCAAATTCGTCAAAGCCTACAATGATGGTGTCAAAAAAGATAAATATTGG
GAATCAACTTTAGAAG----------