>VFA234 (VFA234Q) /CSM/VF/VFA2-B/VFA234Q.Seq.d/
XXXXXXXXXXACCATCGTTTGGAATGGTCCTATGGGTGTATTTGAAAAGAGCAATTTNGN
AGCCGGTTCAAAAGCTGCCATGGATGATGTTGNCAATGCCACAACCAATGGTGCTATCAC
NATCATTGGNGGTGGTGATACCGCCACTTGTGCTGCCAAATACAACACTGAAGATAAAGT
TAGTCATGTCTCAACTGGTGGTGGTGCTTCATTAGNATTATTANAAGGTAAAGAANTACC
AGGTGTNACTGCTTTAT

---IVWNGPMGVFEKSNXXAGSKAAMDDVXNATTNGAITIIGGGDTATCAAKYNTEDKVS
HVSTGGGASLXLLXGKEXPGVTAL

Frame A:
---psfgmvlwvylkraixxpvqklpwmmlxmpqpmvlsxslxvvipplvlpnttlkikl
vmsqlvvvlh*xyxkvkxyqvxlly


Frame B:
---hrlewsygci*keqfxsrfkschg*cxqchnqwcyhxhwxw*yrhlccqiqh*r*s*
sclnwwwcfixiixr*rxtrcxcf


Frame C:
---IVWNGPMGVFEKSNXXAGSKAAMDDVXNATTNGAITIIGGGDTATCAAKYNTEDKVS
HVSTGGGASLXLLXGKEXPGVTAL

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA234 (VFA234Q) /CSM/VF/VFA2-B/VFA234Q.Seq.d/                        436   e-122
VSI344 (VSI344Q) /CSM/VS/VSI3-B/VSI344Q.Seq.d/                        428   e-119
VSD369 (VSD369Q) /CSM/VS/VSD3-C/VSD369Q.Seq.d/                        428   e-119
VHD789 (VHD789Q) /CSM/VH/VHD7-D/VHD789Q.Seq.d/                        428   e-119
VFO594 (VFO594Q) /CSM/VF/VFO5-D/VFO594Q.Seq.d/                        428   e-119
VFN471 (VFN471Q) /CSM/VF/VFN4-C/VFN471Q.Seq.d/                        428   e-119
VFL513 (VFL513Q) /CSM/VF/VFL5-A/VFL513Q.Seq.d/                        428   e-119
VFL140 (VFL140Q) /CSM/VF/VFL1-B/VFL140Q.Seq.d/                        428   e-119
VFK529 (VFK529Q) /CSM/VF/VFK5-B/VFK529Q.Seq.d/                        428   e-119
VFK253 (VFK253Q) /CSM/VF/VFK2-C/VFK253Q.Seq.d/                        428   e-119

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF018637|AF018637.1 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, partial cds.   428  e-116  1
AF316577|AF316577.2 Dictyostelium discoideum phosphoglycerate kinase (pgkA) mRNA, complete cds.   428  e-116  1
D12474|D12474.1 Candida maltosa C-PGK1 gene for phosphoglycerate kinase, complete cds.    80  2e-16  2
AL403633|AL403633.1 T7 end of clone AT0AA009H08 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    74  2e-13  2
BU715686|BU715686.1 SJMCHE04 SJM Schistosoma japonicum cDNA, mRNA sequence.    58  3e-12  2
AX488918|AX488918.1 Sequence 6218 from Patent WO02053728.         64  3e-10  2
BU776741|BU776741.1 SJEDCC02 SJE Schistosoma japonicum cDNA, mRNA sequence.    50  4e-10  2
U25180|U25180.1 Candida albicans phosphoglycerate kinase (PGK1) gene, complete cds.    64  4e-10  2
BU766685|BU766685.1 SJEAIG07 SJE Schistosoma japonicum cDNA, mRNA sequence.    50  5e-10  2
BU771972|BU771972.1 SJEELA04 SJE Schistosoma japonicum cDNA, mRNA sequence.    50  6e-10  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9GPM4) RecName: Full=Phosphoglycerate kinase;          EC=2.7....    85   9e-16
AF018637_1(AF018637|pid:none) Dictyostelium discoideum phosphogl...    85   9e-16
AY309061_1(AY309061|pid:none) Schistosoma japonicum phosphoglyce...    68   9e-11
FN315990_1(FN315990|pid:none) Schistosoma japonicum isolate Anhu...    68   9e-11
EZ000104_1(EZ000104|pid:none) TSA: Schistosoma japonicum SJCHGC0...    68   9e-11
AY223444_1(AY223444|pid:none) Schistosoma japonicum clone ZZZ452...    68   9e-11
FN322120_1(FN322120|pid:none) Schistosoma japonicum isolate Anhu...    68   9e-11
FN315993_1(FN315993|pid:none) Schistosoma japonicum isolate Anhu...    66   3e-10
(P41759) RecName: Full=Phosphoglycerate kinase;          EC=2.7....    66   3e-10
DQ112667_1(DQ112667|pid:none) Fasciola hepatica phosphoglycerate...    65   6e-10

 psg: 0.75  gvh: 0.37  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFA234 is nuc

>VFA234Z.Seq
----------ACCATCGTTTGGAATGGTCCTATGGGTGTATTTGAAAAGAGCAATTTNGN
AGCCGGTTCAAAAGCTGCCATGGATGATGTTGNCAATGCCACAACCAATGGTGCTATCAC
NATCATTGGNGGTGGTGATACCGCCACTTGTGCTGCCAAATACAACACTGAAGATAAAGT
TAGTCATGTCTCAACTGGTGGTGGTGCTTCATTAGNATTATTANAAGGTAAAGAANTACC
AGGTGTNACTGCTTTAT