>VFA223 (VFA223Q) /CSM/VF/VFA2-A/VFA223Q.Seq.d/
XXXXXXXXXXCAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAA
ATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTT
TTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAAT
ATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACAACAGAGGTTTAGCTCAA
TTAGCCGATAAAACTGGTTCAGCCGTAACTGATATTGAAAAATTCTGCATTTGGGGTAAC
CACTCTGCCACCCAATACCCAGATATTAACTTTGGTACCGTCAAAGGTAAATCACTCGTA
GATACCATCAACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGA
GGTGCCGCTATTATTGCTGCCCGTGGTTTATCATCAGCTGCCTCTGCTGCTTCAGCTGCC
ATCGATCACATGAGAGATTGGACCTATGGTACCAATGGTCAATGGACCTCTATGGCCATT
TACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTA
GATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTNATCAATTCTCTCAAGAA

---RSKGMERGDLLKANAEIFSVQGKALDKSANRDTLRVLVVGNPANTNALIAARNAPNI
DPKRFSAMTRLDHNRGLAQLADKTGSAVTDIEKFCIWGNHSATQYPDINFGTVKGKSLVD
TINDQKWVKDNFIPTVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGQWTSMAIY
SEGEYGADKGLYFSFPVIVDNKGKYEIVKGLKLINSLK

Frame A:
---kkqgygaw*pfkskc*nflcsr*sirqkc*s*hfksfscr*psqyqrincrp*ctky
*pktflchdsfrsqqrfssisr*nwfsrn*y*kilhlg*plchpipry*lwyrqr*itrr
yhqrskmg*r*lysnrstkrcryyccpwfiisclccfschrsherldlwyqwsmdlyghl
l*r*iwcr*rslllfpshcr*qr*irnr*rs*txqfsqe


Frame B:
---RSKGMERGDLLKANAEIFSVQGKALDKSANRDTLRVLVVGNPANTNALIAARNAPNI
DPKRFSAMTRLDHNRGLAQLADKTGSAVTDIEKFCIWGNHSATQYPDINFGTVKGKSLVD
TINDQKWVKDNFIPTVQQRGAAIIAARGLSSAASAASAAIDHMRDWTYGTNGQWTSMAIY
SEGEYGADKGLYFSFPVIVDNKGKYEIVKGLKLINSLK


Frame C:
---earvwsvvtf*kqmlkfslfkvkh*tkvlivtl*ef*l*vtqpipth*lppvmhqil
tqnvslp*lv*ittev*ln*piklvqp*lilknsafgvttlppntqiltlvpskvnhs*i
pstiknglkitlfqpfnkevpllllpvvyhqlplllqlpsit*eigpmvpmvngplwpft
lkvnmvpikvstslsqsl*itkvntkslkvlnxsilsr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA223 (VFA223Q) /CSM/VF/VFA2-A/VFA223Q.Seq.d/                       1281   0.0
VSD532 (VSD532Q) /CSM/VS/VSD5-B/VSD532Q.Seq.d/                       1267   0.0
VFI317 (VFI317Q) /CSM/VF/VFI3-A/VFI317Q.Seq.d/                       1267   0.0
VFH801 (VFH801Q) /CSM/VF/VFH8-A/VFH801Q.Seq.d/                       1267   0.0
VFF865 (VFF865Q) /CSM/VF/VFF8-C/VFF865Q.Seq.d/                       1267   0.0
VFF391 (VFF391Q) /CSM/VF/VFF3-D/VFF391Q.Seq.d/                       1267   0.0
VFD443 (VFD443Q) /CSM/VF/VFD4-B/VFD443Q.Seq.d/                       1267   0.0
VFC762 (VFC762Q) /CSM/VF/VFC7-C/VFC762Q.Seq.d/                       1267   0.0
VFB446 (VFB446Q) /CSM/VF/VFB4-B/VFB446Q.Seq.d/                       1267   0.0
VFB440 (VFB440Q) /CSM/VF/VFB4-B/VFB440Q.Seq.d/                       1267   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX685430|AX685430.1 Sequence 43 from Patent WO02090383.           42  1e-04  3
AF288743|AF288743.1 Trichomonas tenax putative cytosolic malate dehydrogenase (MDH) mRNA, partial cds.    38  1e-04  3
AX067436|AX067436.1 Sequence 11 from Patent WO0078968.            42  0.015  3
AF288746|AF288746.2 Hypotrichomonas acosta putative cytosolic malate dehydrogenase (MDH) mRNA, complete cds.    34  0.28  3
BM393456|BM393456.1 50072-2-1-B11.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    38  0.53  2
U38692|U38692.1 Trichomonas vaginalis cytosolic malate dehydrogenase gene, complete cds.    46  0.55  1
CD766976|CD766976.1 AGENCOURT_14738088 NICHD_Rh_Test1 Macaca mulatta cDNA clone IMAGE:6972628 5', mRNA sequence.    46  0.55  1
CD766800|CD766800.1 AGENCOURT_14708832 NICHD_Rh_Test1 Macaca mulatta cDNA clone IMAGE:6972656 5', mRNA sequence.    46  0.55  1
AF288748|AF288748.1 Monocercomonas colubrorum putative cytosolic malate dehydrogenase (MDH) mRNA, partial cds.    36  0.69  2
AY087986|AY087986.1 Arabidopsis thaliana clone 40174 mRNA, complete sequence.    42  0.86  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54D04) RecName: Full=Probable malate dehydrogenase 2, mitochon...   379   e-104
(A6SY47) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   221   1e-56
(Q0ABE6) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   221   1e-56
(Q3BWU8) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   221   2e-56
(Q9K3J3) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   219   4e-56
(A1UZ10) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   218   9e-56
(A3M928) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   218   9e-56
(A5WGM2) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   218   1e-55
(Q0BAF9) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   218   1e-55
(Q393V1) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   218   2e-55

 psg: 0.65  gvh: 0.52  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFA223 is nuc

>VFA223Z.Seq
----------CAAGAAGCAAGGGTATGGAGCGTGGTGACCTTTTAAAAGCAAATGCTGAA
ATTTTCTCTGTTCAAGGTAAAGCATTAGACAAAAGTGCTAATCGTGACACTTTAAGAGTT
TTAGTTGTAGGTAACCCAGCCAATACCAACGCATTAATTGCCGCCCGTAATGCACCAAAT
ATTGACCCAAAACGTTTCTCTGCCATGACTCGTTTAGATCACAACAGAGGTTTAGCTCAA
TTAGCCGATAAAACTGGTTCAGCCGTAACTGATATTGAAAAATTCTGCATTTGGGGTAAC
CACTCTGCCACCCAATACCCAGATATTAACTTTGGTACCGTCAAAGGTAAATCACTCGTA
GATACCATCAACGATCAAAAATGGGTTAAAGATAACTTTATTCCAACCGTTCAACAAAGA
GGTGCCGCTATTATTGCTGCCCGTGGTTTATCATCAGCTGCCTCTGCTGCTTCAGCTGCC
ATCGATCACATGAGAGATTGGACCTATGGTACCAATGGTCAATGGACCTCTATGGCCATT
TACTCTGAAGGTGAATATGGTGCCGATAAAGGTCTCTACTTCTCTTTCCCAGTCATTGTA
GATAACAAAGGTAAATACGAAATCGTTAAAGGTCTTAAACTNATCAATTCTCTCAAGAA