>VFA222 (VFA222Q) /CSM/VF/VFA2-A/VFA222Q.Seq.d/
XXXXXXXXXXCAGTTTTACCAGTTCGTATTATCTTGTTGCTATTTGGTGCAGGTGCTGTC
GAAGGTTCATGTCGTTGGTGGTCAAGAGATCATCGTGCTCATCATCGTTATCTCGATACT
GATAAAGATCCATATTCAGCTTCATTGGGTTTCTGGCATTCCCATTTCATTTGGTTAATG
TTCAAACAAGATCCAAAGAAAATCGGTAGAGCAAACATTGACGACCTTAACGCCGATCCA
TGGGTTATGTGGCAACATAAACATTACATTAAAATCGCCACTTTTATGGGTCTCGTTTTC
CCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGGGCTGGTGGTTATTTCTACTCTGGT
GTACTTCGTCTCGTTACAGTTCAACATTCCACATTCTTGGTCAATTCATTGGCTCATTAT
TTAGGTGACTCTCCATACGATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATA
TTAACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAAC
GCTTATAAATTCTATCAATATGATCCAACTAAATGGTT

---VLPVRIILLLFGAGAVEGSCRWWSRDHRAHHRYLDTDKDPYSASLGFWHSHFIWLMF
KQDPKKIGRANIDDLNADPWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFYSGV
LRLVTVQHSTFLVNSLAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNA
YKFYQYDPTKW

Frame A:
---sftssyylvaiwcrccrrfmslvvkrsscssslsry**rsifsfigflafpfhlvnv
qtrskenr*skh*rp*rrsmgyvat*tlh*nrhfygsrfpnigswfrlg*lgwwlfllwc
tssrysstfhilgqfigslfr*lsir**tyskgfscdcyiniw*rls*lps*ipk*l*kr
l*ilsi*sn*mv


Frame B:
---VLPVRIILLLFGAGAVEGSCRWWSRDHRAHHRYLDTDKDPYSASLGFWHSHFIWLMF
KQDPKKIGRANIDDLNADPWVMWQHKHYIKIATFMGLVFPTLVAGLGWGDWAGGYFYSGV
LRLVTVQHSTFLVNSLAHYLGDSPYDDEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNA
YKFYQYDPTKW


Frame C:
---fyqfvlsccylvqvlskvhvvggqeiivliivisilikihiqlhwvsgipisfg*cs
nkiqrksveqtlttltpihglcgninitlkspllwvsfsqhw*lv*vgviglvvistlvy
fvslqfniphswsihwlii*vtlhtmmnilqriql*lly*hlvkviitsimnsqmtietl
insinmiqlng

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP359 (VHP359Q) /CSM/VH/VHP3-C/VHP359Q.Seq.d/                       1126   0.0
VHP282 (VHP282Q) /CSM/VH/VHP2-D/VHP282Q.Seq.d/                       1126   0.0
VHI633 (VHI633Q) /CSM/VH/VHI6-B/VHI633Q.Seq.d/                       1126   0.0
VFA222 (VFA222Q) /CSM/VF/VFA2-A/VFA222Q.Seq.d/                       1126   0.0
VFM428 (VFM428Q) /CSM/VF/VFM4-B/VFM428Q.Seq.d/                       1090   0.0
VHK887 (VHK887Q) /CSM/VH/VHK8-D/VHK887Q.Seq.d/                       1072   0.0
VHE611 (VHE611Q) /CSM/VH/VHE6-A/VHE611Q.Seq.d/                       1066   0.0
VHL555 (VHL555Q) /CSM/VH/VHL5-C/VHL555Q.Seq.d/                       1053   0.0
VHB725 (VHB725Q) /CSM/VH/VHB7-B/VHB725Q.Seq.d/                       1047   0.0
VHJ240 (VHJ240Q) /CSM/VH/VHJ2-B/VHJ240Q.Seq.d/                       1041   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.  1126  0.0  1
AF026401|AF026401.1 Mucor rouxii stearoyl-CoA desaturase (Ole1) gene, complete cds.    42  8e-09  5
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                    72  8e-09  1
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    54  3e-07  2
BM634131|BM634131.1 17000668813253 A.Gam.ad.cDNA1 Anopheles gambiae cDNA clone 19600448419669 5', mRNA sequence.    54  1e-05  2
J05676|J05676.1 Saccharomyces cerevisiae delta-9 fatty acid desaturase (OLE1) gene, complete cds.    60  3e-05  1
AQ874658|AQ874658.1 V112D8 mTn-3xHA/lacZ Insertion Library, strain Y2278 Saccharomyces cerevisiae genomic 5', genomic survey sequence.    60  3e-05  1
AR016873|AR016873.1 Sequence 1 from patent US 5777201.            60  3e-05  1
Z72577|Z72577.1 S.cerevisiae chromosome VII reading frame ORF YGL055w.    60  3e-05  1
AL392980|AL392980.1 T3 end of clone AR0AA008A11 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    44  2e-04  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   399   e-110
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   286   2e-76
EU857727_1(EU857727|pid:none) Hortaea werneckii delta-9 desatura...   286   3e-76
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   285   5e-76
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   283   2e-75
S52746(S52746;S52745) stearoyl-CoA 9-desaturase (EC 1.14.19.1) [...   282   5e-75
(Q12618) RecName: Full=Acyl-CoA desaturase;          EC=1.14.19....   280   3e-74
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   278   6e-74
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   278   6e-74
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   278   8e-74

 psg: 0.97  gvh: 0.74  alm: 0.32  top: 0.57  tms: 0.07  mit: 0.29  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 1.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: extracellular, including cell wall
	 32.0 %: plasma membrane
	 24.0 %: endoplasmic reticulum
	 12.0 %: Golgi

>> prediction for VFA222 is exc

>VFA222Z.Seq
----------CAGTTTTACCAGTTCGTATTATCTTGTTGCTATTTGGTGCAGGTGCTGTC
GAAGGTTCATGTCGTTGGTGGTCAAGAGATCATCGTGCTCATCATCGTTATCTCGATACT
GATAAAGATCCATATTCAGCTTCATTGGGTTTCTGGCATTCCCATTTCATTTGGTTAATG
TTCAAACAAGATCCAAAGAAAATCGGTAGAGCAAACATTGACGACCTTAACGCCGATCCA
TGGGTTATGTGGCAACATAAACATTACATTAAAATCGCCACTTTTATGGGTCTCGTTTTC
CCAACATTGGTAGCTGGTTTAGGTTGGGGTGATTGGGCTGGTGGTTATTTCTACTCTGGT
GTACTTCGTCTCGTTACAGTTCAACATTCCACATTCTTGGTCAATTCATTGGCTCATTAT
TTAGGTGACTCTCCATACGATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATA
TTAACATTTGGTGAAGGTTATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAAC
GCTTATAAATTCTATCAATATGATCCAACTAAATGGTT