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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA209
Library VF
(Link to library)
Clone ID VFA209
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFA209P
(Link to Original site)
Representative DNA sequence 
>VFA209 (VFA209Q) /CSM/VF/VFA2-A/VFA209Q.Seq.d/
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATT
ATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTAT
TTTAGGTCTTGCTTTCCAAXXXXXXXXXXTTATCACAAGGTTTAGTTTCATCAACACTCT
TCCTCNTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTNTCATTCGGTTCA
ATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTAT
TGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACT
ACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGNTGGTCCAATGGCATGATAT
TACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGA
TTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGT
TTTAACTCCAAAAGAATACGTTTTANAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAG
TGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCAT
CTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTNGGTTTCGCAACTGCCAT
TCAAGGTTAGATTTTTTAATTATTTATATTTAAGATAGAAAGNAAACNAAAATAGAACAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGI
LGLAFQ---

---ITRFSFINTLPXSGYQELQVPTVVNXHSVQSITPNTLVTLPTSH*ptkpignslwmt
llsmvnqlvsvvllvtqfaiqvhhslxvqwhditalneklgavilngegvfsdcsvintl
pnvtitvagrefvltpkeyvlxvtefgkteclsgfmgielnmgnfwilgdvfisayytvf
dfgnkqvgfataiqg*if*lfifkiexkxk*n
Translated Amino Acid sequence (All Frames) 
Frame A:
nchltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwy
hnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrc
lkhicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtrycfrfrqirwy
frscfp---

---lsqglvsstlflxlviknsrcqrw*txirfnr*hqihw*hylrpinqrnllgiryg*
lcyrwsiswflwyylsrnlrfryithxwsngmillpsmkn*vlss*mvkvsslivalstp
yqmlpspllvvnlf*lqkntfxkllsserlnv*vdlwvss*tweisgslvmfsslltily
silvinkxvsqlpfkvrffnylylr*kxnxnrt

Frame B:
ivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGI
LGLAFQ---

---yhkv*fhqhsssfwlsrtpganggelsfgsidntkytgdityvpltnetywefvmdd
faidgqsagfcgttchaicdsgtslixgpma*yycpq*kircchlkw*rcll*l*ryqhl
tkcyhhrcws*icfnskrirfxsy*vrkd*mfewiygyrvkhgkfldpw*cfhlcllyci
rfw**tsxfrnchsrldfliiyi*drkxtkieq

Frame C:
lspdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvp
qsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavp
qahmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpnqvllsispnsmvf
*vlls---

---ITRFSFINTLPXSGYQELQVPTVVNXHSVQSITPNTLVTLPTSH*ptkpignslwmt
llsmvnqlvsvvllvtqfaiqvhhslxvqwhditalneklgavilngegvfsdcsvintl
pnvtitvagrefvltpkeyvlxvtefgkteclsgfmgielnmgnfwilgdvfisayytvf
dfgnkqvgfataiqg*if*lfifkiexkxk*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA209 (VFA209Q) /CSM/VF/VFA2-A/VFA209Q.Seq.d/                       2278   0.0
VFJ894 (VFJ894Q) /CSM/VF/VFJ8-D/VFJ894Q.Seq.d/                       1146   0.0
SFG205 (SFG205Q) /CSM/SF/SFG2-A/SFG205Q.Seq.d/                       1124   0.0
SFG219 (SFG219Q) /CSM/SF/SFG2-A/SFG219Q.Seq.d/                       1122   0.0
SFB424 (SFB424Q) /CSM/SF/SFB4-A/SFB424Q.Seq.d/                       1122   0.0
VFK481 (VFK481Q) /CSM/VF/VFK4-D/VFK481Q.Seq.d/                       1120   0.0
VFE217 (VFE217Q) /CSM/VF/VFE2-A/VFE217Q.Seq.d/                       1120   0.0
CFG766 (CFG766Q) /CSM/CF/CFG7-C/CFG766Q.Seq.d/                       1118   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1116   0.0
VFM401 (VFM401Q) /CSM/VF/VFM4-A/VFM401Q.Seq.d/                       1116   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  1094  0.0  5
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   936  0.0  6
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62  2e-05  1
AL161500|AL161500.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 12.    62  2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  2e-05  1
dna update 2003. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   179   2e-84
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   165   2e-83
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   169   2e-82
AF420068_1(AF420068|pid:none) Clonorchis sinensis aspartic prote...   167   1e-78
AF454831_1(AF454831|pid:none) Apriona germari cathepsin D mRNA, ...   170   6e-76
EF193385_1(EF193385|pid:none) Musca domestica aspartic proteinas...   171   1e-75
AB078420_1(AB078420|pid:none) Brugia malayi asp-2 mRNA for aspar...   153   2e-75
BC154315_1(BC154315|pid:none) Danio rerio cathepsin D, mRNA (cDN...   168   5e-75
AY050516_1(AY050516|pid:none) Danio rerio cathepsin D precursor ...   168   5e-75
BT043515_1(BT043515|pid:none) Salmo salar clone HM4_0887 catheps...   169   6e-75
protein update 2009. 6.16
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: endoplasmic reticulum

>> prediction for VFA209 is exc
5' end seq. ID VFA209F
5' end seq. 
>VFA209F.Seq
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATT
ATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTAT
TTTAGGTCTTGCTTTCCAA----------
Length of 5' end seq. 559
3' end seq. ID VFA209Z
3' end seq. 
>VFA209Z.Seq
----------TTATCACAAGGTTTAGTTTCATCAACACTCTTCCTCNTTCTGGTTATCAA
GAACTCCAGGTGCCAACGGTGGTGAACTNTCATTCGGTTCAATCGATAACACCAAATACA
CTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATG
ACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCG
ATTCAGGTACATCACTCATTGNTGGTCCAATGGCATGATATTACTGCCCTCAATGAAAAA
TTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACC
TTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATAC
GTTTTANAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAG
TTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTA
TTCGATTTTGGTAATAAACAAGTNGGTTTCGCAACTGCCATTCAAGGTTAGATTTTTTAA
TTATTTATATTTAAGATAGAAAGNAAACNAAAATAGAACAA
Length of 3' end seq. 631
Connected seq. ID VFA209P
Connected seq. 
>VFA209P.Seq
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATT
ATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTAT
TTTAGGTCTTGCTTTCCAA----------TTATCACAAGGTTTAGTTTCATCAACACTCT
TCCTCNTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTNTCATTCGGTTCA
ATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTAT
TGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACT
ACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGNTGGTCCAATGGCATGATAT
TACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGA
TTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGT
TTTAACTCCAAAAGAATACGTTTTANAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAG
TGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCAT
CTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTNGGTTTCGCAACTGCCAT
TCAAGGTTAGATTTTTTAATTATTTATATTTAAGATAGAAAGNAAACNAAAATAGAACAA
Length of connected seq. 1190
Full length Seq ID -
Full length Seq. -
Length of full length seq. -