>VFA184 (VFA184Q) /CSM/VF/VFA1-D/VFA184Q.Seq.d/
AATTGTNCCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCT
ACTATTGTCTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAA
TCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACC
ACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACC
CCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCA
AAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCC
TCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATG
TCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTA
TTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATT
TTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATG
TTATCACAAGGTTTAGTTTCATCAACACTCTTCTCXXXXXXXXXX

ncx*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGI
LGLAFQSISVNSIPPVFYNMLSQGLVSSTLF---

Frame A:
ncx*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGI
LGLAFQSISVNSIPPVFYNMLSQGLVSSTLF---


Frame B:
ivpdlhkrk*nylf*lyf*llls*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvp
qsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavp
qahmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpnqvllsispnsmvf
*vllsnpslliqfhqsfttcyhkv*fhqhss---


Frame C:
lxltyikeneityfnfifsyyclsssfnstiklpssfkri*kksstkmvkqiicsqcwyh
npnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrcl
khicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtrycfrfrqirwyf
rscfpihlc*fnstsllqhvitrfsfintl---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA184 (VFA184Q) /CSM/VF/VFA1-D/VFA184Q.Seq.d/                       1253   0.0
VFJ222 (VFJ222Q) /CSM/VF/VFJ2-A/VFJ222Q.Seq.d/                       1245   0.0
VFJ113 (VFJ113Q) /CSM/VF/VFJ1-A/VFJ113Q.Seq.d/                       1245   0.0
VFB255 (VFB255Q) /CSM/VF/VFB2-C/VFB255Q.Seq.d/                       1245   0.0
VFB231 (VFB231Q) /CSM/VF/VFB2-B/VFB231Q.Seq.d/                       1245   0.0
VFA146 (VFA146Q) /CSM/VF/VFA1-B/VFA146Q.Seq.d/                       1245   0.0
VFO689 (VFO689Q) /CSM/VF/VFO6-D/VFO689Q.Seq.d/                       1237   0.0
VFN780 (VFN780Q) /CSM/VF/VFN7-D/VFN780Q.Seq.d/                       1237   0.0
VFN669 (VFN669Q) /CSM/VF/VFN6-C/VFN669Q.Seq.d/                       1237   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       1237   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  1237  0.0  1
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1088  0.0  2
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36  4e-06  4
CB814835|CB814835.1 USDA-FP_100247 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-04_A10 5', mRNA sequence.    36  7e-06  3
AX059531|AX059531.1 Sequence 264 from Patent WO0055325.           62  9e-06  1
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62  9e-06  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62  9e-06  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  9e-06  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  9e-06  1
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62  9e-06  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   209   6e-53
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   207   3e-52
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   206   5e-52
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   205   8e-52
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   205   1e-51
CP001577_36(CP001577|pid:none) Micromonas sp. RCC299 chromosome ...   204   2e-51
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   202   7e-51
EF193385_1(EF193385|pid:none) Musca domestica aspartic proteinas...   199   7e-50
CP000591_303(CP000591|pid:none) Ostreococcus lucimarinus CCE9901...   198   1e-49
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   197   2e-49

 psg: 0.98  gvh: 0.77  alm: 0.41  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: endoplasmic reticulum

>> prediction for VFA184 is exc

>VFA184F.Seq
AATTGTNCCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCT
ACTATTGTCTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAA
TCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACC
ACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACC
CCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCA
AAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCC
TCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATG
TCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTA
TTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATT
TTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATG
TTATCACAAGGTTTAGTTTCATCAACACTCTTCTC----------