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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA171
Library VF
(Link to library)
Clone ID VFA171
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13892-1
Original site URL
Representative seq. ID VFA171P
(Link to Original site)
Representative DNA sequence 
>VFA171 (VFA171Q) /CSM/VF/VFA1-C/VFA171Q.Seq.d/
AAGGTAAAGGTATTAGATTCTATTCATCTTCTAGTCAATTGGATTCAGAATATATGTTTC
CATCAGTCAGAAGATTATTAGTAGAATATGGTATCAATAGTAGTAAGGAAGTAACAGCTA
CAGGTCCACAAAATAGATTACTTAAAGGTGATGTTTTAGCATATATAAAAACAAAGAATT
TATCACCAGTAGATAGATTATCATTAATTGCATCATCAGTAAAATCATCACAACCATCAT
CATCATCATCACCATCAATAGTAGACTCACCAACATTAACATCACAAATTAAAGATCAAA
TTAAAATTGTAACAACAATTACAAATGATAAAAATAAATCAAAAGTTATATACCGAAGAT
ATACCAAATAATAATATTAGAAGAGTAATTGCAACTAAATTAAGTCAATCGAAACAACAA
GTACCACATTTCTATATGACAGTTGAATGTGAATTGGATAATGTTTTAGCAATGAGAAAA
TCTATGCCAGAGAATGTAAAGATCTCTGTAAACGATTTCGTGTTAAGAGCATGTGCATTG
GCATTACGTGATAATCXXXXXXXXXXGTCCGACGAACATGGTGAAGCCATATTAAATCCA
ACCGTTGACATTTCATTTGCAGTTAGTACCGATCGTGGTTTAATCACACCAATCATCACA
AATACCGATAAGAAACAATTGTTGGCAATTTCAAACGAGTCTAAACAATTGGCATTGAAA
GCAAGAGATGGTAAATTGAAACCAGAAGAATTCATTGGTGGTACATTTAGTGTATCAAAT
TTAGGTATGTTTGGTATTACATCCTTTAATGCAATCATAAATTATCCACAAGCTGGTATC
TTGGCAATTGGTACTGGTAGAAAAGTTTTAAGACCACCTTCAACCTATCAACCAATAGAA
ACAAACTTAAACGCATCATATGGCTCTGAAGGTAAATCCGAAATCATAAATACAAATGGT
CCATTAACAACTGAACAATTAGAAAAATTATTTGATAATACTGTATCAAAAAAACAAGAT
ATTAAACAACAAATCATTAATGAACAACCACAACCACCAAAATATGAACAACCAAAGGTT
GCAAATGTTATGGATGTTACACTCTCTGGTATAATAGAGTATTCGANANAAATCGCTGGT
AATTCTATCATCATTTAAATATTATCAAATCCACAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kvkvldsihllvnwiqnicfhqsedy**nmvsivvrk*qlqvhkidylkvmf*hi*kqri
yhq*idyh*lhhq*nhhnhhhhhhhq**thqh*hhklkiklkl*QQLQMIKINQKLYTED
IPNNNIRRVIATKLSQSKQQVPHFYMTVECELDNVLAMRKSMPENVKISVNDFVLRACAL
ALRDN---

---SDEHGEAILNPTVDISFAVSTDRGLITPIITNTDKKQLLAISNESKQLALKARDGKL
KPEEFIGGTFSVSNLGMFGITSFNAIINYPQAGILAIGTGRKVLRPPSTYQPIETNLNAS
YGSEGKSEIINTNGPLTTEQLEKLFDNTVSKKQDIKQQIINEQPQPPKYEQPKVANVMDV
TLSGIIEYSXXIAGNSIII*ilsnpq
Translated Amino Acid sequence (All Frames) 
Frame A:
kvkvldsihllvnwiqnicfhqsedy**nmvsivvrk*qlqvhkidylkvmf*hi*kqri
yhq*idyh*lhhq*nhhnhhhhhhhq**thqh*hhklkiklkl*QQLQMIKINQKLYTED
IPNNNIRRVIATKLSQSKQQVPHFYMTVECELDNVLAMRKSMPENVKISVNDFVLRACAL
ALRDN---

---vrrtw*shiksnr*hfics*yrswfnhtnhhkyr*etivgnfkrv*tigieskrw*i
etrrihwwyi*cikfryvwyyil*cnhklstswylgnwyw*ksfkttfnlstnrnklkri
iwl*r*irnhkykwsinn*tirkii**ycikktry*ttnh**tttttki*ttkgckcygc
ytlwynrvfxxnrw*fyhhlniikstk

Frame B:
r*ry*ilfif*sigfriyvsisqkiisriwyq***gsnsyrstk*it*r*cfsiyknkef
itsr*iiinciiskiittiiiiitinsrltninitn*rsn*ncnnnyk**k*iksyipki
yqiiilee*lqln*vnrnnkyhisi*qlnvnwimf*q*enlcqrm*rsl*tisc*ehvhw
hyvii---

---SDEHGEAILNPTVDISFAVSTDRGLITPIITNTDKKQLLAISNESKQLALKARDGKL
KPEEFIGGTFSVSNLGMFGITSFNAIINYPQAGILAIGTGRKVLRPPSTYQPIETNLNAS
YGSEGKSEIINTNGPLTTEQLEKLFDNTVSKKQDIKQQIINEQPQPPKYEQPKVANVMDV
TLSGIIEYSXXIAGNSIII*ilsnpq

Frame C:
gkgirfyssssqldseymfpsvrrllveyginsskevtatgpqnrllkgdvlayiktknl
spvdrlsliassvkssqpssssspsivdsptltsqikdqikivttitndknkskviyrry
tk**y*ksncn*iksiettsttflyds*m*ig*cfsnekiyareckdlckrfrvksmcig
it**---

---ptnmvkpy*iqpltfhlqlvpivv*shqssqipirnncwqfqtslnnwh*kqemvn*
nqknslvvhlvyqi*vclvlhplmqs*iihklvswqlvlvekf*dhlqpinq*kqt*thh
malkvnpks*iqmvh*qlnn*knyliilyqknkilnnkslmnnhnhqnmnnqrlqmlwml
hslv**sirxkslvilssfkyyqihk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA171 (VFA171Q) /CSM/VF/VFA1-C/VFA171Q.Seq.d/                       1713   0.0
VHN213 (VHN213Q) /CSM/VH/VHN2-A/VHN213Q.Seq.d/                       1100   0.0
VFA484 (VFA484Q) /CSM/VF/VFA4-D/VFA484Q.Seq.d/                       1082   0.0
VFK633 (VFK633Q) /CSM/VF/VFK6-B/VFK633Q.Seq.d/                       1070   0.0
AFE605 (AFE605Q) /CSM/AF/AFE6-A/AFE605Q.Seq.d/                       1070   0.0
AFC534 (AFC534Q) /CSM/AF/AFC5-B/AFC534Q.Seq.d/                       1070   0.0
VFO762 (VFO762Q) /CSM/VF/VFO7-C/VFO762Q.Seq.d/                       1068   0.0
VFC635 (VFC635Q) /CSM/VF/VFC6-B/VFC635Q.Seq.d/                       1066   0.0
AFO171 (AFO171Q) /CSM/AF/AFO1-C/AFO171Q.Seq.d/                       1063   0.0
VFK604 (VFK604Q) /CSM/VF/VFK6-A/VFK604Q.Seq.d/                       1061   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.   874  0.0  9
AE014094|AE014094.1 Buchnera aphidicola str. Sg (Schizaphis graminum) section 19 of 57 of the complete genome.    42  0.48  2
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    36  0.58  9
AC021853|AC021853.3 Homo sapiens chromosome 11 clone RP11-496C3, WORKING DRAFT SEQUENCE, 17 unordered pieces.    36  0.78  4
CB839842|CB839842.1 Ac164 rat regenerating liver after partial hepatectomy Rattus norvegicus cDNA, mRNA sequence.    36  0.79  2
CB839821|CB839821.1 Ac126 rat regenerating liver after partial hepatectomy Rattus norvegicus cDNA, mRNA sequence.    36  0.79  2
BX294107|BX294107.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38  0.85  4
AC144505|AC144505.7 Pan troglodytes clone rp43-17i16, WORKING DRAFT SEQUENCE, 11 unordered pieces.    46  0.99  1
AC046142|AC046142.28 Homo sapiens 3 BAC RP11-550P14 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    46  0.99  1
AC113158|AC113158.2 Mus musculus chromosome UNK clone RP23-101D11, WORKING DRAFT SEQUENCE, 63 unordered pieces.    46  0.99  1
dna update 2003. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK228742_1(AK228742|pid:none) Arabidopsis thaliana mRNA for dihy...   182   2e-44
(Q0WQF7) RecName: Full=Dihydrolipoyllysine-residue acetyltransfe...   182   2e-44
A55939(A55939) dihydrolipoamide S-acetyltransferase (EC 2.3.1.12...   180   8e-44
CU459003_1536(CU459003|pid:none) Magnetospirillum gryphiswaldens...   177   5e-43
EF677914_1(EF677914|pid:none) Picea sitchensis clone WS0278_F17 ...   177   8e-43
AK072554_1(AK072554|pid:none) Oryza sativa Japonica Group cDNA c...   175   3e-42
AP007255_2318(AP007255|pid:none) Magnetospirillum magneticum AMB...   175   3e-42
CP000613_110(CP000613|pid:none) Rhodospirillum centenum SW, comp...   173   9e-42
EF078692_10(EF078692|pid:none) Azoarcus anaerobius transposase g...   167   7e-40
CP000009_2225(CP000009|pid:none) Gluconobacter oxydans 621H, com...   167   7e-40
protein update 2009. 6.16
PSORT 

 psg: 0.59  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFA171 is cyt
5' end seq. ID VFA171F
5' end seq. 
>VFA171F.Seq
AAGGTAAAGGTATTAGATTCTATTCATCTTCTAGTCAATTGGATTCAGAATATATGTTTC
CATCAGTCAGAAGATTATTAGTAGAATATGGTATCAATAGTAGTAAGGAAGTAACAGCTA
CAGGTCCACAAAATAGATTACTTAAAGGTGATGTTTTAGCATATATAAAAACAAAGAATT
TATCACCAGTAGATAGATTATCATTAATTGCATCATCAGTAAAATCATCACAACCATCAT
CATCATCATCACCATCAATAGTAGACTCACCAACATTAACATCACAAATTAAAGATCAAA
TTAAAATTGTAACAACAATTACAAATGATAAAAATAAATCAAAAGTTATATACCGAAGAT
ATACCAAATAATAATATTAGAAGAGTAATTGCAACTAAATTAAGTCAATCGAAACAACAA
GTACCACATTTCTATATGACAGTTGAATGTGAATTGGATAATGTTTTAGCAATGAGAAAA
TCTATGCCAGAGAATGTAAAGATCTCTGTAAACGATTTCGTGTTAAGAGCATGTGCATTG
GCATTACGTGATAATC----------
Length of 5' end seq. 556
3' end seq. ID VFA171Z
3' end seq. 
>VFA171Z.Seq
----------GTCCGACGAACATGGTGAAGCCATATTAAATCCAACCGTTGACATTTCAT
TTGCAGTTAGTACCGATCGTGGTTTAATCACACCAATCATCACAAATACCGATAAGAAAC
AATTGTTGGCAATTTCAAACGAGTCTAAACAATTGGCATTGAAAGCAAGAGATGGTAAAT
TGAAACCAGAAGAATTCATTGGTGGTACATTTAGTGTATCAAATTTAGGTATGTTTGGTA
TTACATCCTTTAATGCAATCATAAATTATCCACAAGCTGGTATCTTGGCAATTGGTACTG
GTAGAAAAGTTTTAAGACCACCTTCAACCTATCAACCAATAGAAACAAACTTAAACGCAT
CATATGGCTCTGAAGGTAAATCCGAAATCATAAATACAAATGGTCCATTAACAACTGAAC
AATTAGAAAAATTATTTGATAATACTGTATCAAAAAAACAAGATATTAAACAACAAATCA
TTAATGAACAACCACAACCACCAAAATATGAACAACCAAAGGTTGCAAATGTTATGGATG
TTACACTCTCTGGTATAATAGAGTATTCGANANAAATCGCTGGTAATTCTATCATCATTT
AAATATTATCAAATCCACAAAA
Length of 3' end seq. 612
Connected seq. ID VFA171P
Connected seq. 
>VFA171P.Seq
AAGGTAAAGGTATTAGATTCTATTCATCTTCTAGTCAATTGGATTCAGAATATATGTTTC
CATCAGTCAGAAGATTATTAGTAGAATATGGTATCAATAGTAGTAAGGAAGTAACAGCTA
CAGGTCCACAAAATAGATTACTTAAAGGTGATGTTTTAGCATATATAAAAACAAAGAATT
TATCACCAGTAGATAGATTATCATTAATTGCATCATCAGTAAAATCATCACAACCATCAT
CATCATCATCACCATCAATAGTAGACTCACCAACATTAACATCACAAATTAAAGATCAAA
TTAAAATTGTAACAACAATTACAAATGATAAAAATAAATCAAAAGTTATATACCGAAGAT
ATACCAAATAATAATATTAGAAGAGTAATTGCAACTAAATTAAGTCAATCGAAACAACAA
GTACCACATTTCTATATGACAGTTGAATGTGAATTGGATAATGTTTTAGCAATGAGAAAA
TCTATGCCAGAGAATGTAAAGATCTCTGTAAACGATTTCGTGTTAAGAGCATGTGCATTG
GCATTACGTGATAATC----------GTCCGACGAACATGGTGAAGCCATATTAAATCCA
ACCGTTGACATTTCATTTGCAGTTAGTACCGATCGTGGTTTAATCACACCAATCATCACA
AATACCGATAAGAAACAATTGTTGGCAATTTCAAACGAGTCTAAACAATTGGCATTGAAA
GCAAGAGATGGTAAATTGAAACCAGAAGAATTCATTGGTGGTACATTTAGTGTATCAAAT
TTAGGTATGTTTGGTATTACATCCTTTAATGCAATCATAAATTATCCACAAGCTGGTATC
TTGGCAATTGGTACTGGTAGAAAAGTTTTAAGACCACCTTCAACCTATCAACCAATAGAA
ACAAACTTAAACGCATCATATGGCTCTGAAGGTAAATCCGAAATCATAAATACAAATGGT
CCATTAACAACTGAACAATTAGAAAAATTATTTGATAATACTGTATCAAAAAAACAAGAT
ATTAAACAACAAATCATTAATGAACAACCACAACCACCAAAATATGAACAACCAAAGGTT
GCAAATGTTATGGATGTTACACTCTCTGGTATAATAGAGTATTCGANANAAATCGCTGGT
AATTCTATCATCATTTAAATATTATCAAATCCACAAAA
Length of connected seq. 1168
Full length Seq ID -
Full length Seq. -
Length of full length seq. -