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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA149
Library VF
(Link to library)
Clone ID VFA149
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16305-1
Original site URL
Representative seq. ID VFA149P
(Link to Original site)
Representative DNA sequence 
>VFA149 (VFA149Q) /CSM/VF/VFA1-C/VFA149Q.Seq.d/
AACTCCCAATTTATNAATTAAACATTGGTGGTATCTCTCATCGTTTCCAAATTNACGATA
AAACTTCCACATCCTACACCCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTAT
CTCCAGAAGAAGATTTACTCTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAA
ACTCTTTAATCTCACCAATGCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATA
AAGTTGTCCTTGGTCAAGAACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCA
GAGCTCCAAAAGATTGTGAAATTAAATCAATCAATGTTAAACCTGGTCAAGTCGTTTCTG
TTGATGAAGTTTTAATTGATTTTAAATAAAAATAAAAATAAAATAAAAATAAAAATAAAA
TAAAATAAAAAAATAAAATAAAAAAAAAAXXXXXXXXXXAACTCCACAATTTATCAAATT
AAACATTGGTGGTATCTCTCATCGTTTCCAAATTCACGATAAAACTTCCACATCCTACAC
CCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTATCTCCAGAAGAAGATTTACT
CTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAAACTCTTTAATCTCACCAAT
GCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATAAAGTTGTCCTTGGTCAAGA
ACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCAGAGCTCCAAAAGATTGTTA
AATTAAATCAATCAATGTTAAACCGGTCAAGTCGTTTCTGTNATAAGTTTNATNATTTTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
lpiyxlniggishrfqixdktstsytlqfhgskipvtvlspeedllcqympvkktvdssn
slispmpgtilslavnvgdkvvlgqelciveamkmqnvlrapkdceiksinvkpgqvvsv
devlidfk*k*k*nknknkik*KNKIKKK---

---TPQFIKLNIGGISHRFQIHDKTSTSYTLQFHGSKIPVTVLSPEEDLLCQYMPVKKTV
DSSNSLISPMPGTILSLAVNVGDKVVLGQELCIVEAMKMQNVLRAPKDC*iksinvkpvk
sflx*vxxf
Translated Amino Acid sequence (All Frames) 
Frame A:
nsqfxn*tlvvslivskxtiklphptpfnsmvqrfqspfylqkkiysantcqlkklltlq
tl*shqclvlsslwplm*viklslvknfvslkq*kckmfselqkivklnqsmlnlvksfl
lmkf*lilnknknkikikik*nkkik*kk---

---nstiyqikhwwylssfpnsr*nfhilhpsipwfkdsshrfisrrrftlpihas*knc
*lfklfnltnawyyplfgr*cr**scpwsrtlyr*snenakcsqsskrlln*inqc*tgq
vvsvisxxil

Frame B:
tpnlxikhwwylssfpnxr*nfhilhpsipwfkdsshrfisrrrftlpihas*knc*lfk
lfnltnawyyplfgr*cr**scpwsrtlyr*snenakcsqsskrl*n*inqc*twssrfc
**sfn*f*ikikik*k*k*nkikk*nkkk---

---TPQFIKLNIGGISHRFQIHDKTSTSYTLQFHGSKIPVTVLSPEEDLLCQYMPVKKTV
DSSNSLISPMPGTILSLAVNVGDKVVLGQELCIVEAMKMQNVLRAPKDC*iksinvkpvk
sflx*vxxf

Frame C:
lpiyxlniggishrfqixdktstsytlqfhgskipvtvlspeedllcqympvkktvdssn
slispmpgtilslavnvgdkvvlgqelciveamkmqnvlrapkdceiksinvkpgqvvsv
devlidfk*k*k*nknknkik*KNKIKKK---

---lhnlsn*tlvvslivskftiklphptpfnsmvqrfqspfylqkkiysantcqlkkll
tlqtl*shqclvlsslwplm*viklslvknfvslkq*kckmfselqkivklnqsmlnrss
rfcxkfxxf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA149 (VFA149Q) /CSM/VF/VFA1-C/VFA149Q.Seq.d/                        704   0.0
VHL103 (VHL103Q) /CSM/VH/VHL1-A/VHL103Q.Seq.d/                        686   0.0
VHM783 (VHM783Q) /CSM/VH/VHM7-D/VHM783Q.Seq.d/                        684   0.0
VHM613 (VHM613Q) /CSM/VH/VHM6-A/VHM613Q.Seq.d/                        682   0.0
VHQ352 (VHQ352Q) /CSM/VH/VHQ3-C/VHQ352Q.Seq.d/                        680   0.0
VHK778 (VHK778Q) /CSM/VH/VHK7-D/VHK778Q.Seq.d/                        680   0.0
AHP613 (AHP613Q) /CSM/AH/AHP6-A/AHP613Q.Seq.d/                        680   0.0
AHL327 (AHL327Q) /CSM/AH/AHL3-B/AHL327Q.Seq.d/                        680   0.0
AHC331 (AHC331Q) /CSM/AH/AHC3-B/AHC331Q.Seq.d/                        680   0.0
AHA745 (AHA745Q) /CSM/AH/AHA7-B/AHA745Q.Seq.d/                        680   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U32778|U32778.1 Haemophilus influenzae Rd section 93 of 163 of the complete genome.    54  0.003  1
AR274513|AR274513.1 Sequence 1 from patent US 6506581.            54  0.003  1
AE006150|AE006150.1 Pasteurella multocida PM70 section 117 of 204 of the complete genome.    48  0.18  2
BG192825|BG192825.1 RST11942 Athersys RAGE Library Homo sapiens cDNA, mRNA sequence.    44  0.18  2
AC131016|AC131016.3 Rattus norvegicus clone CH230-57L9, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    46  0.70  1
AC125908|AC125908.3 Rattus norvegicus clone CH230-70G12, WORKING DRAFT SEQUENCE, 1 ordered piece.    46  0.70  1
AC131605|AC131605.4 Rattus norvegicus clone CH230-2D8, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    46  0.70  1
AC112782|AC112782.3 Rattus norvegicus clone CH230-unknown, WORKING DRAFT SEQUENCE, 1 ordered piece.    46  0.70  1
BX324177|BX324177.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-23F9.    46  0.78  2
CB311297|CB311297.1 AGENCOURT_11616492 NICHD_Rh_Ov1 Macaca mulatta cDNA clone IMAGE:6894088 5', mRNA sequence.    36  0.84  2
dna update 2003. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000830_719(CP000830|pid:none) Dinoroseobacter shibae DFL 12, c...    94   6e-18
CP000927_3152(CP000927|pid:none) Caulobacter sp. K31, complete g...    91   4e-17
CP000927_2989(CP000927|pid:none) Caulobacter sp. K31, complete g...    91   4e-17
CP000490_835(CP000490|pid:none) Paracoccus denitrificans PD1222 ...    90   8e-17
FN357591_3(FN357591|pid:none) Schistosoma mansoni genome sequenc...    90   8e-17
FN357591_4(FN357591|pid:none) Schistosoma mansoni genome sequenc...    90   8e-17
CP000628_1885(CP000628|pid:none) Agrobacterium radiobacter K84 c...    89   2e-16
CP000377_1869(CP000377|pid:none) Silicibacter sp. TM1040, comple...    89   2e-16
CP000874_2061(CP000874|pid:none) Rhizobium sp. NGR234 plasmid pN...    88   3e-16
(Q19842) RecName: Full=Propionyl-CoA carboxylase alpha chain, mi...    88   4e-16
protein update 2009. 6.16
PSORT 

 psg: 0.68  gvh: 0.32  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VFA149 is cyt
5' end seq. ID VFA149F
5' end seq. 
>VFA149F.Seq
AACTCCCAATTTATNAATTAAACATTGGTGGTATCTCTCATCGTTTCCAAATTNACGATA
AAACTTCCACATCCTACACCCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTAT
CTCCAGAAGAAGATTTACTCTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAA
ACTCTTTAATCTCACCAATGCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATA
AAGTTGTCCTTGGTCAAGAACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCA
GAGCTCCAAAAGATTGTGAAATTAAATCAATCAATGTTAAACCTGGTCAAGTCGTTTCTG
TTGATGAAGTTTTAATTGATTTTAAATAAAAATAAAAATAAAATAAAAATAAAAATAAAA
TAAAATAAAAAAATAAAATAAAAAAAAAA----------
Length of 5' end seq. 449
3' end seq. ID VFA149Z
3' end seq. 
>VFA149Z.Seq
----------AACTCCACAATTTATCAAATTAAACATTGGTGGTATCTCTCATCGTTTCC
AAATTCACGATAAAACTTCCACATCCTACACCCTTCAATTCCATGGTTCAAAGATTCCAG
TCACCGTTTTATCTCCAGAAGAAGATTTACTCTGCCAATACATGCCAGTTAAAAAAACTG
TTGACTCTTCAAACTCTTTAATCTCACCAATGCCTGGTACTATCCTCTCTTTGGCCGTTA
ATGTAGGTGATAAAGTTGTCCTTGGTCAAGAACTTTGTATCGTTGAAGCAATGAAAATGC
AAAATGTTCTCAGAGCTCCAAAAGATTGTTAAATTAAATCAATCAATGTTAAACCGGTCA
AGTCGTTTCTGTNATAAGTTTNATNATTTTA
Length of 3' end seq. 381
Connected seq. ID VFA149P
Connected seq. 
>VFA149P.Seq
AACTCCCAATTTATNAATTAAACATTGGTGGTATCTCTCATCGTTTCCAAATTNACGATA
AAACTTCCACATCCTACACCCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTAT
CTCCAGAAGAAGATTTACTCTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAA
ACTCTTTAATCTCACCAATGCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATA
AAGTTGTCCTTGGTCAAGAACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCA
GAGCTCCAAAAGATTGTGAAATTAAATCAATCAATGTTAAACCTGGTCAAGTCGTTTCTG
TTGATGAAGTTTTAATTGATTTTAAATAAAAATAAAAATAAAATAAAAATAAAAATAAAA
TAAAATAAAAAAATAAAATAAAAAAAAAA----------AACTCCACAATTTATCAAATT
AAACATTGGTGGTATCTCTCATCGTTTCCAAATTCACGATAAAACTTCCACATCCTACAC
CCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTATCTCCAGAAGAAGATTTACT
CTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAAACTCTTTAATCTCACCAAT
GCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATAAAGTTGTCCTTGGTCAAGA
ACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCAGAGCTCCAAAAGATTGTTA
AATTAAATCAATCAATGTTAAACCGGTCAAGTCGTTTCTGTNATAAGTTTNATNATTTTA
Length of connected seq. 830
Full length Seq ID -
Full length Seq. -
Length of full length seq. -