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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA143
Library VF
(Link to library)
Clone ID VFA143
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16243-1
Original site URL
Representative seq. ID VFA143P
(Link to Original site)
Representative DNA sequence 
>VFA143 (VFA143Q) /CSM/VF/VFA1-B/VFA143Q.Seq.d/
ATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTNAAGAATTGA
AACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATC
AGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGC
TAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACA
TATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGT
ATTACCAAGATCATCACAAGGTGCGACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTT
ACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAAT
TGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGT
AGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGA
GGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGA
GCGTTTTGGTGTCACCAGXXXXXXXXXXAATGTCGCAGAGCGTTTTGGTGTCACCAGAAA
AGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGG
TAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGT
CGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAA
ACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGG
TGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGA
ATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCC
AGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGT
TTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTT
GGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGC
TACTGGTGCCCGTCAAAGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATAG
GTGTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
YLYIILHPITNKMSFNXRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKA
KRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGL
PKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQE
DPIISGCYNTMGQTSENVAERFGVT---

---NVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEG
IREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAF
AAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVN
PNGSGIALGHPLGATGARQRLPFQENYIVVKVNRC
Translated Amino Acid sequence (All Frames) 
Frame A:
ifiynitphn**nviqxkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl*s
*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcdrs*schiisrf
tkgstmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmgwwfqrgcpr
gsnh*wllqhygsnl*kcrrafwchq---

---NVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEG
IREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAF
AAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVN
PNGSGIALGHPLGATGARQRLPFQENYIVVKVNRC

Frame B:
YLYIILHPITNKMSFNXRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKA
KRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGL
PKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQE
DPIISGCYNTMGQTSENVAERFGVT---

---msqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssiktkv
svrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*ll
llsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrfwvwi*tkli
qmalvsl*vih*vllvpvkgyhfkritss*r*igv

Frame C:
iyi*yytp*likchsixelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqfvkl
keehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvrpklelphy*qvy
qrkyhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmvvstrlpkr
iqslvvvttlwvkplkmsqsvlvsp---

---crrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rrry
p*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrsfc
ccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs*s
kwlwyrfrssircywcpskatisrelhrrkgk*v
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA143 (VFA143Q) /CSM/VF/VFA1-B/VFA143Q.Seq.d/                       2442   0.0
CFB766 (CFB766Q) /CSM/CF/CFB7-C/CFB766Q.Seq.d/                       1257   0.0
SFL240 (SFL240Q) /CSM/SF/SFL2-B/SFL240Q.Seq.d/                       1251   0.0
AFJ691 (AFJ691Q) /CSM/AF/AFJ6-D/AFJ691Q.Seq.d/                       1251   0.0
SFF320 (SFF320Q) /CSM/SF/SFF3-A/SFF320Q.Seq.d/                       1245   0.0
SFF124 (SFF124Q) /CSM/SF/SFF1-A/SFF124Q.Seq.d/                       1245   0.0
VFL606 (VFL606Q) /CSM/VF/VFL6-A/VFL606Q.Seq.d/                       1237   0.0
VFJ317 (VFJ317Q) /CSM/VF/VFJ3-A/VFJ317Q.Seq.d/                       1237   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1237   0.0
VFD865 (VFD865Q) /CSM/VF/VFD8-C/VFD865Q.Seq.d/                       1237   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1168  0.0  4
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60  1e-13  5
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44  6e-13  5
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60  7e-12  4
CF213585|CF213585.1 CGF1000794_F07 Vitis vinifera cv. cabernet sauvignon Stem - CAST Vitis vinifera cDNA clone CAST0006_IF_F07 5', mRNA sequence.    60  3e-10  3
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68  6e-10  3
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60  1e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68  1e-08  2
CD716621|CD716621.1 VVB129E11_405541 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB129E11 5, mRNA sequence.    60  1e-08  2
CD008871|CD008871.1 VVB097G11_339261 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB097G11 5, mRNA sequence.    60  1e-08  2
dna update 2003. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   709   0.0
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   394   e-108
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   394   e-108
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   392   e-107
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   389   e-107
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   387   e-106
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   387   e-106
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   385   e-105
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   385   e-105
BT070433_1(BT070433|pid:none) Picea sitchensis clone WS02729_F15...   384   e-105
protein update 2009. 6.16
PSORT 

 psg: 0.87  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.31  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFA143 is cyt
5' end seq. ID VFA143F
5' end seq. 
>VFA143F.Seq
ATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTNAAGAATTGA
AACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATC
AGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGC
TAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACA
TATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGT
ATTACCAAGATCATCACAAGGTGCGACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTT
ACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAAT
TGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGT
AGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGA
GGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGA
GCGTTTTGGTGTCACCAG----------
Length of 5' end seq. 618
3' end seq. ID VFA143Z
3' end seq. 
>VFA143Z.Seq
----------AATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCTC
TGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAAT
CGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGG
TATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGG
TGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGT
TATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTT
TGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGC
CGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGC
TTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAA
TCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAAG
GCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATAGGTGTA
Length of 3' end seq. 637
Connected seq. ID VFA143P
Connected seq. 
>VFA143P.Seq
ATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTNAAGAATTGA
AACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATC
AGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGC
TAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACA
TATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGT
ATTACCAAGATCATCACAAGGTGCGACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTT
ACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAAT
TGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGT
AGAATCAATGAGTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGA
GGATCCAATCATTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAAAATGTCGCAGA
GCGTTTTGGTGTCACCAG----------AATGTCGCAGAGCGTTTTGGTGTCACCAGAAA
AGACCAAGATGAATTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGG
TAAATTTGACGATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGT
CGTCGATAAAGACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAA
ACCAGCTTTCAAAGAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGG
TGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGA
ATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCC
AGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGT
TTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTT
GGATATGAACAAAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGC
TACTGGTGCCCGTCAAAGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATAG
GTGTA
Length of connected seq. 1255
Full length Seq ID -
Full length Seq. -
Length of full length seq. -