NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFA114
Library VF
(Link to library)
Clone ID VFA114
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13991-1
Original site URL
Representative seq. ID VFA114P
(Link to Original site)
Representative DNA sequence 
>VFA114 (VFA114Q) /CSM/VF/VFA1-A/VFA114Q.Seq.d/
TAGAATCCACACATTATAATTTTATAATGGGAAAAAGAAAAACTATGCAATCATTTAACC
TTGAATCCTTTGGAAAAAATTTAGAAAAAAAACTTAGACGAAATCCAGAACAACTACAAC
AACCAATTAAAAATTATAAATCCGAAAGAGAAAGAGATATCCATGAATTAATTTATACAA
TTAAATCGGAAAGAATGTTTATTTATAAAAATGCTCATAGACATCTTAAATTGGCTTTGG
AAAATGCTACTCTTGCGTTGAATTTACCAATGCTCCTAAAAGACTTCCATGTAACATTGA
AAGAATTTGATGCTCTTGAAGCCAGTTTGAATGCTGAGCTAGAGTGTTTGGAATTACAAT
ATTCTAGCGATACATCAGAATTACTTTTACCAGTTAATAGTGTTAATACCAGTCAAAACA
CAATCAATGAAAACGCCATTACCACAGCCATCGCCTCATTATCAGTTAACCCTGTTAATA
CCAGTGTTGCTCTTTCCACTGCTTCCACTTCTACTTCAACTCCCACTAACACTACCACTA
CTACTACCCACCACTTCCAXXXXXXXXXXCACTACCACTACTACTACCACCACTTCCAAT
TCACTTACCAAAAATAATAATTCCGCCTTAGTTTCAAAACCAAAAACACGTGGCGTACGT
TCCAAACCAATTGATATGAATAGTAGTGATGATGAAGAAGATGATCAAAAAGATGATCAA
GACAAAGATGATAGTGATGAAGACAATGTCGATAATACTCCACCATTGGATTCAAATGAT
TCTAAACCACCATCATCAAAAACAAAAGGAATTTCCAAAACCAAGACAAAAGGAAATGTT
AGCACTGCCATTACCACCACCACCACTCCCATTACTACTACTGACTCCAATATTATCGGT
ACTACTACAACCACTGATGATATCACAGAAGAAAGCAAGGTAAAAGAAAGACCACCAAGG
ATATTTCCAGAAAATTGCTATGTCTGTAAAGTAACCGAAACTCCATATTGGAGAAGAGGT
ACAGATAATGGTGTTGTAGTTGACCTATGCAATGAATGTGGACTATACTACATGAATAAA
GAAAAGAAAGAGAGACTTTCCAGACAAAAACATAGTATCAAAAATGTTTTGAACNACCTC
TTTCTAAATCTTTTTTACTTTTCCAACATTTTTTTAATCAAATTCATTTTTNANATTTTT
T
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ESTHYNFIMGKRKTMQSFNLESFGKNLEKKLRRNPEQLQQPIKNYKSERERDIHELIYTI
KSERMFIYKNAHRHLKLALENATLALNLPMLLKDFHVTLKEFDALEASLNAELECLELQY
SSDTSELLLPVNSVNTSQNTINENAITTAIASLSVNPVNTSVALSTASTSTSTPTNTTTT
TTHHF---

---TTTTTTTTSNSLTKNNNSALVSKPKTRGVRSKPIDMNSSDDEEDDQKDDQDKDDSDE
DNVDNTPPLDSNDSKPPSSKTKGISKTKTKGNVSTAITTTTTPITTTDSNIIGTTTTTDD
ITEESKVKERPPRIFPENCYVCKVTETPYWRRGTDNGVVVDLCNECGLYYMNKEKKERLS
RQKHSIKNVLNXLFLNLFYFSNIFLIKFIFXIF
Translated Amino Acid sequence (All Frames) 
Frame A:
*nphiiil*wekeklcnhltlnpleki*kknldeiqnnynnqlkiinpkekeismn*fiq
lnrkeclfikmlidilnwlwkmlllr*iyqcs*ktsm*h*knlmllkpv*mls*svwnyn
ilaihqnyfyqlivlipvktqsmktplpqpsphyqltllipvllfpllplllqlpltlpl
llptts---

---hyhyyyhhfqftyqk**frlsfktkntwrtfqtn*ye*****rr*skr*srqr****
rqcr*ystigfk*f*ttiiknkrnfqnqdkrkc*hchyhhhhshyyy*lqyyryyynh**
yhrrkqgkrkttkdisrkllcl*snrnsilekryr*wccs*pmq*mwtilhe*rkeretf
qtkt*yqkcfexplsksfllfqhffnqihfxxf

Frame B:
rihtl*fyngkkknyaii*p*ilwkkfrkkt*tksrtttttn*kl*irkrkryp*inlyn
*igknvyl*kcs*ts*igfgkcyscveftnapkrlpcnieri*cs*sqfec*arvfgiti
f*ryiritfts**c*yqskhnq*krhyhshrliis*pc*yqccsfhcfhfyfnsh*hyhy
yypplp---

---TTTTTTTTSNSLTKNNNSALVSKPKTRGVRSKPIDMNSSDDEEDDQKDDQDKDDSDE
DNVDNTPPLDSNDSKPPSSKTKGISKTKTKGNVSTAITTTTTPITTTDSNIIGTTTTTDD
ITEESKVKERPPRIFPENCYVCKVTETPYWRRGTDNGVVVDLCNECGLYYMNKEKKERLS
RQKHSIKNVLNXLFLNLFYFSNIFLIKFIFXIF

Frame C:
ESTHYNFIMGKRKTMQSFNLESFGKNLEKKLRRNPEQLQQPIKNYKSERERDIHELIYTI
KSERMFIYKNAHRHLKLALENATLALNLPMLLKDFHVTLKEFDALEASLNAELECLELQY
SSDTSELLLPVNSVNTSQNTINENAITTAIASLSVNPVNTSVALSTASTSTSTPTNTTTT
TTHHF---

---lplllpplpihlpkiiipp*fqnqkhvayvpnqli*ivvmmkkmikkmiktkmivmk
tmsiilhhwiqmilnhhhqkqkefpkprqkemlalplpppplplllltpilsvllqplmi
sqkkar*kkdhqgyfqkiamsvk*pklhigeevqimvl*ltyamnvdytt*ikkrkrdfp
dknivskmf*ttsf*ifftfptff*snsfxxff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFA114 (VFA114Q) /CSM/VF/VFA1-A/VFA114Q.Seq.d/                       1096   0.0
VFM220 (VFM220Q) /CSM/VF/VFM2-A/VFM220Q.Seq.d/                       1074   0.0
SFE256 (SFE256Q) /CSM/SF/SFE2-C/SFE256Q.Seq.d/                       1074   0.0
AFO747 (AFO747Q) /CSM/AF/AFO7-B/AFO747Q.Seq.d/                       1072   0.0
VFG133 (VFG133Q) /CSM/VF/VFG1-B/VFG133Q.Seq.d/                       1047   0.0
VFL145 (VFL145Q) /CSM/VF/VFL1-B/VFL145Q.Seq.d/                        952   0.0
VSG594 (VSG594Q) /CSM/VS/VSG5-D/VSG594Q.Seq.d/                        823   0.0
VSC323 (VSC323Q) /CSM/VS/VSC3-A/VSC323Q.Seq.d/                        135   2e-30
VHC635 (VHC635Q) /CSM/VH/VHC6-B/VHC635Q.Seq.d/                        129   1e-28
VFH458 (VFH458Q) /CSM/VF/VFH4-C/VFH458Q.Seq.d/                        105   2e-21
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    38  0.006  11
AZ551092|AZ551092.1 ENTFJ22TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    48  0.011  2
AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.    44  0.020  8
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    36  0.023  9
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    38  0.027  11
CC875501|CC875501.1 ZMMBBb0181K22.f ZMMBBb Zea mays subsp. mays genomic clone ZMMBBb0181K22 5', genomic survey sequence.    42  0.033  2
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    36  0.043  12
AZ551618|AZ551618.1 ENTDV54TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50  0.065  1
BH160272|BH160272.1 ENTQV49TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    50  0.065  1
BI175597|BI175597.1 OSTR050B1_1 AD-wrmcDNA Caenorhabditis elegans cDNA similar to F53A2.9, mRNA sequence.    50  0.065  1
dna update 2003. 8.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54L80) RecName: Full=GATA zinc finger domain-containing protei...   262   2e-68
(Q54WY0) RecName: Full=GATA zinc finger domain-containing protei...    72   5e-11
(Q54US7) RecName: Full=GATA zinc finger domain-containing protei...    67   1e-09
(Q54X31) RecName: Full=Putative GATA zinc finger domain-containi...    63   2e-08
(Q54FV1) RecName: Full=GATA zinc finger domain-containing protei...    57   1e-06
(Q54V32) RecName: Full=GATA zinc finger domain-containing protei...    55   5e-06
(Q54L83) RecName: Full=GATA zinc finger domain-containing protei...    54   8e-06
(Q54TE3) RecName: Full=GATA zinc finger domain-containing protei...    51   9e-05
AL670009_23(AL670009|pid:none) Neurospora crassa DNA linkage gro...    48   6e-04
(Q75JZ0) RecName: Full=GATA zinc finger domain-containing protei...    48   6e-04
protein update 2009. 6.16
PSORT 

 psg: 0.54  gvh: 0.28  alm: 0.30  top: 0.23  tms: 0.07  mit: 0.28  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 16.0 %: vesicles of secretory system
	 12.0 %: endoplasmic reticulum
	 12.0 %: plasma membrane
	  8.0 %: Golgi
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VFA114 is nuc
5' end seq. ID VFA114F
5' end seq. 
>VFA114F.Seq
TAGAATCCACACATTATAATTTTATAATGGGAAAAAGAAAAACTATGCAATCATTTAACC
TTGAATCCTTTGGAAAAAATTTAGAAAAAAAACTTAGACGAAATCCAGAACAACTACAAC
AACCAATTAAAAATTATAAATCCGAAAGAGAAAGAGATATCCATGAATTAATTTATACAA
TTAAATCGGAAAGAATGTTTATTTATAAAAATGCTCATAGACATCTTAAATTGGCTTTGG
AAAATGCTACTCTTGCGTTGAATTTACCAATGCTCCTAAAAGACTTCCATGTAACATTGA
AAGAATTTGATGCTCTTGAAGCCAGTTTGAATGCTGAGCTAGAGTGTTTGGAATTACAAT
ATTCTAGCGATACATCAGAATTACTTTTACCAGTTAATAGTGTTAATACCAGTCAAAACA
CAATCAATGAAAACGCCATTACCACAGCCATCGCCTCATTATCAGTTAACCCTGTTAATA
CCAGTGTTGCTCTTTCCACTGCTTCCACTTCTACTTCAACTCCCACTAACACTACCACTA
CTACTACCCACCACTTCCA----------
Length of 5' end seq. 559
3' end seq. ID VFA114Z
3' end seq. 
>VFA114Z.Seq
----------CACTACCACTACTACTACCACCACTTCCAATTCACTTACCAAAAATAATA
ATTCCGCCTTAGTTTCAAAACCAAAAACACGTGGCGTACGTTCCAAACCAATTGATATGA
ATAGTAGTGATGATGAAGAAGATGATCAAAAAGATGATCAAGACAAAGATGATAGTGATG
AAGACAATGTCGATAATACTCCACCATTGGATTCAAATGATTCTAAACCACCATCATCAA
AAACAAAAGGAATTTCCAAAACCAAGACAAAAGGAAATGTTAGCACTGCCATTACCACCA
CCACCACTCCCATTACTACTACTGACTCCAATATTATCGGTACTACTACAACCACTGATG
ATATCACAGAAGAAAGCAAGGTAAAAGAAAGACCACCAAGGATATTTCCAGAAAATTGCT
ATGTCTGTAAAGTAACCGAAACTCCATATTGGAGAAGAGGTACAGATAATGGTGTTGTAG
TTGACCTATGCAATGAATGTGGACTATACTACATGAATAAAGAAAAGAAAGAGAGACTTT
CCAGACAAAAACATAGTATCAAAAATGTTTTGAACNACCTCTTTCTAAATCTTTTTTACT
TTTCCAACATTTTTTTAATCAAATTCATTTTTNANATTTTTT
Length of 3' end seq. 632
Connected seq. ID VFA114P
Connected seq. 
>VFA114P.Seq
TAGAATCCACACATTATAATTTTATAATGGGAAAAAGAAAAACTATGCAATCATTTAACC
TTGAATCCTTTGGAAAAAATTTAGAAAAAAAACTTAGACGAAATCCAGAACAACTACAAC
AACCAATTAAAAATTATAAATCCGAAAGAGAAAGAGATATCCATGAATTAATTTATACAA
TTAAATCGGAAAGAATGTTTATTTATAAAAATGCTCATAGACATCTTAAATTGGCTTTGG
AAAATGCTACTCTTGCGTTGAATTTACCAATGCTCCTAAAAGACTTCCATGTAACATTGA
AAGAATTTGATGCTCTTGAAGCCAGTTTGAATGCTGAGCTAGAGTGTTTGGAATTACAAT
ATTCTAGCGATACATCAGAATTACTTTTACCAGTTAATAGTGTTAATACCAGTCAAAACA
CAATCAATGAAAACGCCATTACCACAGCCATCGCCTCATTATCAGTTAACCCTGTTAATA
CCAGTGTTGCTCTTTCCACTGCTTCCACTTCTACTTCAACTCCCACTAACACTACCACTA
CTACTACCCACCACTTCCA----------CACTACCACTACTACTACCACCACTTCCAAT
TCACTTACCAAAAATAATAATTCCGCCTTAGTTTCAAAACCAAAAACACGTGGCGTACGT
TCCAAACCAATTGATATGAATAGTAGTGATGATGAAGAAGATGATCAAAAAGATGATCAA
GACAAAGATGATAGTGATGAAGACAATGTCGATAATACTCCACCATTGGATTCAAATGAT
TCTAAACCACCATCATCAAAAACAAAAGGAATTTCCAAAACCAAGACAAAAGGAAATGTT
AGCACTGCCATTACCACCACCACCACTCCCATTACTACTACTGACTCCAATATTATCGGT
ACTACTACAACCACTGATGATATCACAGAAGAAAGCAAGGTAAAAGAAAGACCACCAAGG
ATATTTCCAGAAAATTGCTATGTCTGTAAAGTAACCGAAACTCCATATTGGAGAAGAGGT
ACAGATAATGGTGTTGTAGTTGACCTATGCAATGAATGTGGACTATACTACATGAATAAA
GAAAAGAAAGAGAGACTTTCCAGACAAAAACATAGTATCAAAAATGTTTTGAACNACCTC
TTTCTAAATCTTTTTTACTTTTCCAACATTTTTTTAATCAAATTCATTTTTNANATTTTT
T
Length of connected seq. 1191
Full length Seq ID -
Full length Seq. -
Length of full length seq. -