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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSM734
Library SS
(Link to library)
Clone ID SSM734
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SSM734P
(Link to Original site)
Representative DNA sequence 
>SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/
CTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAA
TCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTT
TACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCA
ACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAA
GGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAA
TCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAAT
AAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGT
GCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATG
AGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTA
GCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGT
ATGGGTTCAGAAGTTTATCATAACTTAAAGAATGTCATCAGTGGTAGATACGGTCAAGAT
GCTATCAATGTTGGTGATGAAXXXXXXXXXXCAGAGAATTCATTAAAGAATATCCAATCA
TTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCAT
CAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGA
CTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAG
TCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTA
GCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTA
CTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAAC
TTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTA
AATTTTAATTTTACTCATAATATGTGAAACAAAACTAAAAAAAAAAAAAGAATAAAAAAA
ATAAAAAAAATAAAAAAGCAAAAAAAAAA
sequence update 1998.12. 3
Translated Amino Acid sequence 
LYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAS
TGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPN
KGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKL
AMQEFMILPVGAKDFNEAYRMGSEVYHNLKNVISGRYGQDAINVGDE---

---REFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKAC
NALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGA
PCRSERLAKYNQLVRINEELGENHNYAGLTFRKF*fys*yvkqn*kkkknkknkknkkak
kk
Translated Amino Acid sequence (All Frames) 
Frame A:
LYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAS
TGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPN
KGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKL
AMQEFMILPVGAKDFNEAYRMGSEVYHNLKNVISGRYGQDAINVGDE---

---qrih*risnhfn*rsi*sr*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm
*csfiks*pnwfsh*inscrs*lkecimgchg*psfr*n*rylhc*fscwfrywsn*nwc
sm*irkisqiqstc*n*rrvr*kp*lcwfnfp*ililliicetklkkkke*kk*kk*ksk
kk

Frame B:
fiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhq
lvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqi
kvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*
lcknl*fyqlvlkismkpivwvqkfiit*rmssvvdtvkmlsmlvm---

---REFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKAC
NALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGA
PCRSERLAKYNQLVRINEELGENHNYAGLTFRKF*fys*yvkqn*kkkknkknkknkkak
kk

Frame C:
lfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircin
wyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk*
r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**is
yariydftswc*rfq*slsygfrsls*lkechqw*irsrcyqcw**---

---enslkniqsfqlkihlikmignhtln*qhqlifkllvmiyllptqkelrlvlkrkhv
mlfy*kltklvqslnqfvplltqrmhhgvswlaivqvklkipslli*llv*vlvklklvl
hvdqkd*pntinlleltks*vktitmlv*lsvnfnfthnm*nktkkkkrikkikkikkqk
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       2232   0.0
AFD204 (AFD204Q) /CSM/AF/AFD2-A/AFD204Q.Seq.d/                       1318   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1310   0.0
SFC211 (SFC211Q) /CSM/SF/SFC2-A/SFC211Q.Seq.d/                       1285   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1267   0.0
AFE445 (AFE445Q) /CSM/AF/AFE4-B/AFE445Q.Seq.d/                       1267   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1265   0.0
AFO860 (AFO860Q) /CSM/AF/AFO8-C/AFO860Q.Seq.d/                       1265   0.0
AFO869 (AFO869Q) /CSM/AF/AFO8-C/AFO869Q.Seq.d/                       1263   0.0
AFG235 (AFG235Q) /CSM/AF/AFG2-B/AFG235Q.Seq.d/                       1257   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          86  1e-67  11
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  4e-58  9
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  4e-58  9
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76  4e-58  9
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76  3e-56  8
X80474|X80474.1 N.frontalis enolase gene.                         64  5e-54  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92  3e-50  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66  2e-46  7
AX489475|AX489475.1 Sequence 6775 from Patent WO02053728.         56  3e-43  10
M93712|M93712.1 Candida albicans (clone lambda-C1.d) enolase mRNA, complete cds.    56  3e-43  10
dna update 2003. 8.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   411   e-113
EF676265_1(EF676265|pid:none) Picea sitchensis clone WS02725_D05...   272   e-109
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   298   2e-79
BT082621_1(BT082621|pid:none) Anoplopoma fimbria clone afim-evh-...   298   3e-79
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   298   3e-79
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   297   6e-79
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   296   8e-79
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   296   8e-79
DQ497985_1(DQ497985|pid:none) Polypterus senegalus enolase A mRN...   295   2e-78
BC071359_1(BC071359|pid:none) Danio rerio enolase 1, (alpha), mR...   295   3e-78
protein update 2009. 6.16
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.33  top: 0.60  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for SSM734 is cyt
5' end seq. ID SSM734F
5' end seq. 
>SSM734F.Seq
CTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAA
TCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTT
TACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCA
ACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAA
GGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAA
TCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAAT
AAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGT
GCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATG
AGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTA
GCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGT
ATGGGTTCAGAAGTTTATCATAACTTAAAGAATGTCATCAGTGGTAGATACGGTCAAGAT
GCTATCAATGTTGGTGATGAA----------
Length of 5' end seq. 681
3' end seq. ID SSM734Z
3' end seq. 
>SSM734Z.Seq
----------CAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTG
ATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTG
GTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCAT
GTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCG
CTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTG
AAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTG
CTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAGT
TAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAAT
ATGTGAAACAAAACTAAAAAAAAAAAAAGAATAAAAAAAATAAAAAAAATAAAAAAGCAA
AAAAAAAA
Length of 3' end seq. 538
Connected seq. ID SSM734P
Connected seq. 
>SSM734P.Seq
CTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAA
TCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTT
TACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCA
ACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAA
GGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAAA
TCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAAT
AAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGGT
GCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAATG
AGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATTA
GCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAGCCTATCGT
ATGGGTTCAGAAGTTTATCATAACTTAAAGAATGTCATCAGTGGTAGATACGGTCAAGAT
GCTATCAATGTTGGTGATGAA----------CAGAGAATTCATTAAAGAATATCCAATCA
TTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCAT
CAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGA
CTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAG
TCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTA
GCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTA
CTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAAC
TTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTA
AATTTTAATTTTACTCATAATATGTGAAACAAAACTAAAAAAAAAAAAAGAATAAAAAAA
ATAAAAAAAATAAAAAAGCAAAAAAAAAA
Length of connected seq. 1219
Full length Seq ID -
Full length Seq. -
Length of full length seq. -