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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSJ256
Library SS
(Link to library)
Clone ID SSJ256
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16278-1
Original site URL
Representative seq. ID SSJ256E
(Link to Original site)
Representative DNA sequence 
>SSJ256 (SSJ256Q) /CSM/SS/SSJ2-C/SSJ256Q.Seq.d/
GTCGGTTTAGCTGCTGCNGGTACAACCCCCCTTTTTTCTCCAAACTCCAATTCGGTGAAA
TTGTTACTACAATTCCACCCATTGGTTTCAATGNCGAAACTGTCGAATTCAAAAACATTC
ACCTCACNGTATGGGATGTTGGTGGTCAAGATAAAACCGTCCATTATGGAGACATTATTT
CCAAAATACTCAAGGTCTTATCTTTGTTGTTGATTCAAACGATAGAGAAAGAATTCAAGA
AGCTTGTGATGAACTCACTAAAATGCTCAATGAAGATGAATTACGTGATGCTGTTTTATT
AGTTTTCTGTAACAAACAAGATCTTCCAAATGCCATGAGTGTCGCTGAAGTTCCCGATAA
ATTAAATCTCCACTCTCTCCGTTCAAGAAAATGGTACATACAGTCAACTTGTGCCACCAG
TGGTGATGGTTTATATGAAGGTTTAGACTGGCTCTCAAATACCTTAACAAGCTCCTCAAA
ATAAATGACGTAGGAGGTCAATTTAATAGAGTTTATTAGATTTTTTAATTATTTAATAAT
CTAATTAAACTACTGTTGTTTAATTTTGAAATAAAAAAAAAAAAAACCTTGTTTATATAA
ATAATAATAAATAAAATAATAATAATAATAAAAAAAAAAAAAAAAAAAGAATCCACTAAA
AATTTTATGTGATTGCACACAAAAA
sequence update 1998. 1. 8
Translated Amino Acid sequence 
sv*llxvqppfflqtpir*ncyynsthwfqxrncriqkhsphxmgcwwsr*NRPLWRHYF
QNTQGLIFVVDSNDRERIQEACDELTKMLNEDELRDAVLLVFCNKQDLPNAMSVAEVPDK
LNLHSLRSRKWYIQSTCATSGDGLYEGLDWLSNTLTSSSK*mt*evnliefirffnylii
*lnycclilk*kkkkpclyk***ik*****kkkkkknplkilcdctqk
Translated Amino Acid sequence (All Frames) 
Frame A:
vglaaagttplfspnsnsvklllqfhplvsmxklsnsktftsxygmlvvkikpsimetlf
pkysrsylcc*fkr*rknsrsl**th*naq*r*it*ccfisfl*qtrsskchecr*ssr*
iksplspfkkmvhtvnlchqw*wfi*rfrlalkylnkllkindvggqfnrvy*if*lfnn
liklllfnfeikkkktlfi*iiinkiiiiikkkkkkestknfm*lhtk


Frame B:
sv*llxvqppfflqtpir*ncyynsthwfqxrncriqkhsphxmgcwwsr*NRPLWRHYF
QNTQGLIFVVDSNDRERIQEACDELTKMLNEDELRDAVLLVFCNKQDLPNAMSVAEVPDK
LNLHSLRSRKWYIQSTCATSGDGLYEGLDWLSNTLTSSSK*mt*evnliefirffnylii
*lnycclilk*kkkkpclyk***ik*****kkkkkknplkilcdctqk


Frame C:
rfsccxynppffsklqfgeivttippigfnxetvefknihltvwdvggqdktvhygdiis
kilkvlsllliqtiekefkklvmnslkcsmkmnyvmlfy*fsvtnkifqmp*vslkfpin
*istlsvqengtysqlvppvvmvymkv*tgsqip*qapqnk*rrrsi**slldflii**s
n*ttvv*f*nkkkknlvyinnnk*nnnnnkkkkkkrih*kfyviahk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSJ256 (SSJ256Q) /CSM/SS/SSJ2-C/SSJ256Q.Seq.d/                       1116   0.0
SSL154 (SSL154Q) /CSM/SS/SSL1-C/SSL154Q.Seq.d/                        969   0.0
SSL113 (SSL113Q) /CSM/SS/SSL1-A/SSL113Q.Seq.d/                        969   0.0
SSK216 (SSK216Q) /CSM/SS/SSK2-A/SSK216Q.Seq.d/                        969   0.0
SSJ475 (SSJ475Q) /CSM/SS/SSJ4-D/SSJ475Q.Seq.d/                        969   0.0
SSI674 (SSI674Q) /CSM/SS/SSI6-D/SSI674Q.Seq.d/                        969   0.0
SSI502 (SSI502Q) /CSM/SS/SSI5-A/SSI502Q.Seq.d/                        969   0.0
SSG749 (SSG749Q) /CSM/SS/SSG7-C/SSG749Q.Seq.d/                        969   0.0
SSF795 (SSF795Q) /CSM/SS/SSF7-D/SSF795Q.Seq.d/                        969   0.0
SSC884 (SSC884Q) /CSM/SS/SSC8-D/SSC884Q.Seq.d/                        969   0.0
own update 2003.10.30
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ000063|AJ000063.1 Dictyostelium discoideum mRNA for ADP-ribosylation factor 1.   815   0.0    2
AB001051|AB001051.1 Dugesia japonica mRNA for ADP-ribosylation factor, complete cds.    84   7e-36  5
CF778427|CF778427.1 tad17b06.x1 Hydra EST -IV Hydra magnipapillata cDNA 3' similar to SW:ARF1_CAEEL Q10943 ADP-RIBOSYLATION FACTOR 1. [1] ;, mRNA sequence.    66   3e-27  4
CF778694|CF778694.1 tad17b06.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:ARF1_CAEEL Q10943 ADP-RIBOSYLATION FACTOR 1. [1] ;, mRNA sequence.    66   4e-27  4
CK150930|CK150930.1 CsmgEST02373 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence.    76   1e-25  4
BE579078|BE579078.1 kq22a04.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to gb:M36341 ADP-RIBOSYLATION FACTOR 4 (HUMAN);, mRNA sequence.    50   6e-25  4
BG225964|BG225964.1 kp44b08.y1 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to gb:M36341 ADP-RIBOSYLATION FACTOR 4 (HUMAN);, mRNA sequence.    60   7e-24  4
L09551|L09551.1 Schizosaccharomyces pombe ADP-ribosylation factor 1 (ARF1) mRNA, complete cds.    62   2e-23  6
BM173409|0    68   2e-23  3
BM878930|BM878930.1 P11-G08 Sweetpotato Ipomoea batatas cDNA similar to ADP-ribosylation factor, mRNA sequence.    60   2e-22  5
dna update 2004. 2.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O00909) RecName: Full=ADP-ribosylation factor 1;  &AJ000063_1(A...   221   6e-71
(P91924) RecName: Full=ADP-ribosylation factor;  &AB001051_1(AB0...   188   2e-60
AC135465_4(AC135465|pid:none) Medicago truncatula clone mth2-24h...   186   7e-60
(Q06396) RecName: Full=ADP-ribosylation factor 1; AltName: Full=...   186   7e-60
AP003570_13(AP003570|pid:none) Oryza sativa Japonica Group genom...   186   9e-60
(P51821) RecName: Full=ADP-ribosylation factor 1;  &S66337(S6633...   186   9e-60
BT065309_1(BT065309|pid:none) Zea mays full-length cDNA clone ZM...   186   9e-60
(O48649) RecName: Full=ADP-ribosylation factor 1;  &AB003377_1(A...   186   1e-59
(P51822) RecName: Full=ADP-ribosylation factor 1;  &D45420_1(D45...   186   1e-59
AK226308_1(AK226308|pid:none) Arabidopsis thaliana mRNA for simi...   185   2e-59
protein update 2009. 7. 8
PSORT 

 psg: 0.89  gvh: 0.33  alm: 0.49  top: 0.27  tms: 0.00  mit: 0.44  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SSJ256 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID SSJ256E
Full length Seq. 
>SSJ256E.Seq
GTCGGTTTAGCTGCTGCNGGTACAACCCCCCTTTTTTCTCCAAACTCCAATTCGGTGAAA
TTGTTACTACAATTCCACCCATTGGTTTCAATGNCGAAACTGTCGAATTCAAAAACATTC
ACCTCACNGTATGGGATGTTGGTGGTCAAGATAAAACCGTCCATTATGGAGACATTATTT
CCAAAATACTCAAGGTCTTATCTTTGTTGTTGATTCAAACGATAGAGAAAGAATTCAAGA
AGCTTGTGATGAACTCACTAAAATGCTCAATGAAGATGAATTACGTGATGCTGTTTTATT
AGTTTTCTGTAACAAACAAGATCTTCCAAATGCCATGAGTGTCGCTGAAGTTCCCGATAA
ATTAAATCTCCACTCTCTCCGTTCAAGAAAATGGTACATACAGTCAACTTGTGCCACCAG
TGGTGATGGTTTATATGAAGGTTTAGACTGGCTCTCAAATACCTTAACAAGCTCCTCAAA
ATAAATGACGTAGGAGGTCAATTTAATAGAGTTTATTAGATTTTTTAATTATTTAATAAT
CTAATTAAACTACTGTTGTTTAATTTTGAAATAAAAAAAAAAAAAACCTTGTTTATATAA
ATAATAATAAATAAAATAATAATAATAATAAAAAAAAAAAAAAAAAAAGAATCCACTAAA
AATTTTATGTGATTGCACACAAAAA
Length of full length seq. 685