>SSJ182 (SSJ182Q) /CSM/SS/SSJ1-D/SSJ182Q.Seq.d/
XXXXXXXXXXTAAGGTATTGTCGGGTATTGCTAAAGGTCCCCCTTCCGGCTGCAGTGCTG
GTCCAGATACGGATGATATTTTCCTTTGGACAGCTACTTTTATGGGTCCAAAAGACTCCC
CTTACGAGGGGGTGTTTTCTTTTGTAATATTAATTTCCCAACGGTTTATCCATTTAACCC
CCCAAGGTTTGGTTTTGTTCCCAGGTTTCCCCTCCAATTTTTAATGCCCGCGGAAGTATT
TGTTTAGATATTTTGGGGGAACAATGGAGTCCAGCCTTAACTATTCCAAAAGTTTTACTT
TCAATTAGTGCCCTTTTATCTGACCCAAATCCAGATGATCCTCTTGTACCAGATATTGCC
AATTTATTTAAAACAGACAAAAATAGATTCGAATCTAATGCCCGTGAGTGGACAAGAAAA
TATGCACAATAATTTTTCACGAATAGATAAGGCAACCCAAAAAAAAAAATTTTTTTTGGT
GAACGACTAAACTTTTAAAATAAACAATAGAAGTTCTGTTACAAATCAAAAAAAAAAAAA
AAAAAAAAAAA

---rycrvllkvplpaavlvqirmifsfgqlllwvqktpltrgcfll*y*fpnglsi*PP
KVWFCSQVSPPIFNARGSICLDILGEQWSPALTIPKVLLSISALLSDPNPDDPLVPDIAN
LFKTDKNRFESNAREWTRKYAQ*fftnr*gnpkkkiffgerlnf*nkq*kfcykskkkkk
kk

Frame A:
---kvlsgiakgppsgcsagpdtddiflwtatfmgpkdspyegvfsfvilisqrfihltp
qglvlfpgfpsnf*cprkylfryfggtmesslnysksftfn*cpfi*pksr*ssctrycq
fi*nrqk*iri*cp*vdkkictiifhe*irqpkkknffw*ttkllk*tievllqikkkkk
kkk


Frame B:
---rycrvllkvplpaavlvqirmifsfgqlllwvqktpltrgcfll*y*fpnglsi*PP
KVWFCSQVSPPIFNARGSICLDILGEQWSPALTIPKVLLSISALLSDPNPDDPLVPDIAN
LFKTDKNRFESNAREWTRKYAQ*fftnr*gnpkkkiffgerlnf*nkq*kfcykskkkkk
kk


Frame C:
---givgyc*rspfrlqcwsryg*yfpldsyfygskrlplrggvffcninfptvypfnpp
rfgfvprfplqflmpaevfv*ifwgnngvqp*lfqkfyfqlvpfyltqiqmillyqilpi
ylkqtkidsnlmpvsgqenmhnnfsridkatqkkkfflvnd*tfkinnrssvtnqkkkkk
kk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSJ182 (SSJ182Q) /CSM/SS/SSJ1-D/SSJ182Q.Seq.d/                        888   0.0
SSC652 (SSC652Q) /CSM/SS/SSC6-C/SSC652Q.Seq.d/                        609   e-173
VSG818 (VSG818Q) /CSM/VS/VSG8-A/VSG818Q.Seq.d/                        601   e-171
VSF889 (VSF889Q) /CSM/VS/VSF8-D/VSF889Q.Seq.d/                        601   e-171
VSC717 (VSC717Q) /CSM/VS/VSC7-A/VSC717Q.Seq.d/                        601   e-171
SSF567 (SSF567Q) /CSM/SS/SSF5-C/SSF567Q.Seq.d/                        601   e-171
AHN338 (AHN338Q) /CSM/AH/AHN3-B/AHN338Q.Seq.d/                        601   e-171
VSJ387 (VSJ387Q) /CSM/VS/VSJ3-D/VSJ387Q.Seq.d/                        587   e-167
VSJ287 (VSJ287Q) /CSM/VS/VSJ2-D/VSJ287Q.Seq.d/                        337   1e-91
SSL483 (SSL483Q) /CSM/SS/SSL4-D/SSL483Q.Seq.d/                        274   1e-72

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BQ046108|BQ046108.1 EST595226 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI13F10 5' end, mRNA sequence.    52   2e-09  4
AW033420|AW033420.1 EST276991 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28F11 similar to ubiquitin carrier protein (Ubc), mRNA sequence.    52   2e-09  4
BE432882|BE432882.1 EST399327 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG10K8, mRNA sequence.    52   2e-09  4
BF153240|P35135, mRNA sequence.    52   2e-09  4
BE342564|BE342564.1 EST395408 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA20O8, mRNA sequence.    52   2e-09  4
AW932719|AW932719.1 EST358562 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF49N9 5', mRNA sequence.    52   3e-09  4
AI898137|AI898137.1 EST267580 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED31H4, mRNA sequence.    52   3e-09  4
AI781804|AI781804.1 EST262683 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES17M3, mRNA sequence.    52   3e-09  4
AW443460|AW443460.1 EST308390 tomato mixed elicitor, BTI Lycopersicon esculentum cDNA clone cLET44C24 5', mRNA sequence.    52   3e-09  4
AW038844|AW038844.1 EST280800 tomato mixed elicitor, BTI Lycopersicon esculentum cDNA clone cLET7P6, mRNA sequence.    52   3e-09  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AX406018_1(AX406018|pid:none) Sequence 433 from Patent WO0222660.     123   4e-37
(P51668) RecName: Full=Ubiquitin-conjugating enzyme E2 D1;      ...   123   4e-37
CR940352_416(CR940352|pid:none) Theileria annulata strain Ankara...   118   3e-34
AY389716_1(AY389716|pid:none) Hyacinthus orientalis ubiquitin-co...   122   2e-33
EF576159_1(EF576159|pid:none) Oryza sativa (indica cultivar-grou...   127   3e-31
DQ144988_1(DQ144988|pid:none) Symbiodinium sp. C3 ubiquitin conj...   105   7e-31
AE017346_284(AE017346|pid:none) Cryptococcus neoformans var. neo...   134   2e-30
(P35133) RecName: Full=Ubiquitin-conjugating enzyme E2 10;      ...   134   2e-30
AF325019_1(AF325019|pid:none) Arabidopsis thaliana AT4g27960 (AT...   133   3e-30
DQ027023_1(DQ027023|pid:none) Arabidopsis thaliana ubiquitinatin...   133   3e-30

 psg: 0.86  gvh: 0.56  alm: 0.37  top: 0.33  tms: 0.00  mit: 0.32  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 24.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SSJ182 is cyt

>SSJ182Z.Seq
----------TAAGGTATTGTCGGGTATTGCTAAAGGTCCCCCTTCCGGCTGCAGTGCTG
GTCCAGATACGGATGATATTTTCCTTTGGACAGCTACTTTTATGGGTCCAAAAGACTCCC
CTTACGAGGGGGTGTTTTCTTTTGTAATATTAATTTCCCAACGGTTTATCCATTTAACCC
CCCAAGGTTTGGTTTTGTTCCCAGGTTTCCCCTCCAATTTTTAATGCCCGCGGAAGTATT
TGTTTAGATATTTTGGGGGAACAATGGAGTCCAGCCTTAACTATTCCAAAAGTTTTACTT
TCAATTAGTGCCCTTTTATCTGACCCAAATCCAGATGATCCTCTTGTACCAGATATTGCC
AATTTATTTAAAACAGACAAAAATAGATTCGAATCTAATGCCCGTGAGTGGACAAGAAAA
TATGCACAATAATTTTTCACGAATAGATAAGGCAACCCAAAAAAAAAAATTTTTTTTGGT
GAACGACTAAACTTTTAAAATAAACAATAGAAGTTCTGTTACAAATCAAAAAAAAAAAAA
AAAAAAAAAAA