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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSJ127
Library SS
(Link to library)
Clone ID SSJ127
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12249-1
Original site URL
Representative seq. ID SSJ127P
(Link to Original site)
Representative DNA sequence 
>SSJ127 (SSJ127Q) /CSM/SS/SSJ1-B/SSJ127Q.Seq.d/
CACTTCATATTTATTTTTTTTTTTTTTTTTCATATATATTATTTATTAAAAGATGACAGA
AAAGCAATTAAGTTGTTGTCTCGATTTAATGAGACGTTTACCACCATCTCAAATTGAAGA
TAATTTAGCAGGTCTCCTTGATCTTGTCCCAGATCTCACTGAAGACCTTTTATCATCAAT
TGATCAACCACTCAAGGTTGCTTATGATGCAGTTTCAAAAAAAGATTATTTATTATGTGA
TTACAATAGAGATGCTGATTCATACAGATCACCATGGTCAAACAAATATGATCCACCATT
AAGTGGTGCATGTTATCCATCATCAAAATTAAGAGATATTGAAGTTCAAGCTAATGAAAT
TTTTGAAATTTATCTTAACTTATATTTTGAAGGTGGTGTTTCATCTGTTTATTGTTGGGA
TCTCGATGATAATTTCGCTGCTGTAGTTTTAATGAAAAAAACTCAAGATCAATCTAAAAA
AGGTCAACCAATGAGAGGTACTTGGGATTCAATTCATGTTGTTGAAGTTAAACTTXXXXX
XXXXXTGCCGACCCCCCACCCCTCACCATCCACCTCAACCGTGGGGAAGGGAGATGGGGG
GTTATACGCGGAATGTGCGACGGTACTAACACCCTTTTCAATGCAATTGTCAATAAAGAG
TTGAGAAAGTTCACTTTTATCCTCATTGTATAGACTAGTTCTAGTATGCGACGAGCGGNC
CGACCACCGACGGTGGCNCTACCAATT
sequence update 1998.12.21
Translated Amino Acid sequence 
tsylffffffiyiiy*KMTEKQLSCCLDLMRRLPPSQIEDNLAGLLDLVPDLTEDLLSSI
DQPLKVAYDAVSKKDYLLCDYNRDADSYRSPWSNKYDPPLSGACYPSSKLRDIEVQANEI
FEIYLNLYFEGGVSSVYCWDLDDNFAAVVLMKKTQDQSKKGQPMRGTWDSIHVVEVKL--
-

---ADPPPLTIHLNRGEGRWGVIRGMCDGTNTLFNAIVNKELRKFTFILIV*tsssmrra
xrpptvalpi
Translated Amino Acid sequence (All Frames) 
Frame A:
hfififffffhiyyllkddrkaikllsrfnetfttisn*r*fsrsp*scprsh*rpfiin
*sttqgcl*csfkkrlfim*lq*rc*fiqitmvkqi*stikwcmlsiikikry*sss**n
f*nls*lif*rwcficlllgsr**frccsfneknsrsi*krstnerylgfnscc*s*t--
-

---crpptphhppqpwgremggytrnvrry*hpfqcncq*rvekvhfyphcid*f*yats
gpttdggxtn

Frame B:
tsylffffffiyiiy*KMTEKQLSCCLDLMRRLPPSQIEDNLAGLLDLVPDLTEDLLSSI
DQPLKVAYDAVSKKDYLLCDYNRDADSYRSPWSNKYDPPLSGACYPSSKLRDIEVQANEI
FEIYLNLYFEGGVSSVYCWDLDDNFAAVVLMKKTQDQSKKGQPMRGTWDSIHVVEVKL--
-

---ADPPPLTIHLNRGEGRWGVIRGMCDGTNTLFNAIVNKELRKFTFILIV*tsssmrra
xrpptvalpi

Frame C:
lhiyfffffsyilfikr*qksn*vvvsi**dvyhhlklkii*qvslilsqislktfyhql
inhsrllmmqfqkkiiyyvitiemlihtdhhgqtnmihh*vvhvihhqn*eilkfklmkf
lkfiltyilkvvfhlfivgismiisll*f**kklkinlkkvnq*evlgiqfmllkln---

---ptphpspststvgkgdgglyaecatvltpfsmqlsiks*essllsslyrlvlvcder
xdhrrwxyq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSJ127 (SSJ127Q) /CSM/SS/SSJ1-B/SSJ127Q.Seq.d/                       1267   0.0
SSA656 (SSA656Q) /CSM/SS/SSA6-C/SSA656Q.Seq.d/                        906   0.0
VSG245 (VSG245Q) /CSM/VS/VSG2-B/VSG245Q.Seq.d/                        819   0.0
FC-IC1128 (FC-IC1128Q) /CSM/FC-IC/FC-IC1128Q.Seq.d/                   795   0.0
VFF819 (VFF819Q) /CSM/VF/VFF8-A/VFF819Q.Seq.d/                        533   e-150
SSK848 (SSK848Q) /CSM/SS/SSK8-B/SSK848Q.Seq.d/                        482   e-135
VFD371 (VFD371Q) /CSM/VF/VFD3-C/VFD371Q.Seq.d/                        446   e-124
SSH682 (SSH682Q) /CSM/SS/SSH6-D/SSH682Q.Seq.d/                        410   e-113
SSL728 (SSL728Q) /CSM/SS/SSL7-B/SSL728Q.Seq.d/                        222   6e-57
SSJ183 (SSJ183Q) /CSM/SS/SSJ1-D/SSJ183Q.Seq.d/                        184   1e-45
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M25131|M25131.1 D.discoideum CAP32 protein, complete cds.         458   0.0    3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   129   6e-26  1
BQ701924|BQ701924.1 NXSI_122_C04_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA clone NXSI_122_C04 5' similar to Arabidopsis thaliana sequence At1g71790 actin capping protein beta-2, putative see http://mips.gsf.de/proj/thal/db/index.html, mRNA sequence.    76   8e-10  1
BI514873|BI514873.1 BB160016B10F10 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160016B10F10 5', mRNA sequence.    44   7e-08  3
CF233520|CF233520.1 PtaJXO0024E7E0709 Poplar cDNA library from young opposite xylem Populus x canescens cDNA 5', mRNA sequence.    54   2e-06  2
CA821313|CA821313.1 RSH01D01 two-month-old roots from clone 'Beaupre' grown for 19 days under restricted irrigation Populus balsamifera subsp. trichocarpa x Populus deltoides cDNA 5', mRNA sequence.    54   2e-06  2
CF234179|CF234179.1 PtaJXO3D9D0907 Poplar cDNA library from young opposite xylem Populus x canescens cDNA 5', mRNA sequence.    54   2e-06  2
BU862607|BU862607.1 S017G10 Populus imbibed seed cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    54   3e-06  2
BU834615|BU834615.1 T063E11 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    54   3e-06  2
BF272695|BF272695.1 GA__Eb0015K12f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Eb0015K12f, mRNA sequence.    64   3e-06  1
dna update 2004. 2.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13021) RecName: Full=F-actin-capping protein subunit beta; Alt...   333   3e-90
BC041233_1(BC041233|pid:none) Xenopus laevis capping protein bet...   228   2e-58
EF011869_1(EF011869|pid:none) Xenopus laevis capping protein bet...   228   2e-58
CR861078_1(CR861078|pid:none) Pongo abelii mRNA; cDNA DKFZp459M1...   226   4e-58
AY890783_1(AY890783|pid:none) Synthetic construct Homo sapiens c...   226   4e-58
AY888202_1(AY888202|pid:none) Synthetic construct Homo sapiens c...   226   4e-58
(Q5R507) RecName: Full=F-actin-capping protein subunit beta; Alt...   226   4e-58
(P47757) RecName: Full=F-actin-capping protein subunit beta; Alt...   226   4e-58
(P47756) RecName: Full=F-actin-capping protein subunit beta; Alt...   226   4e-58
AL035413_2(AL035413|pid:none) Human DNA sequence from clone RP4-...   224   2e-57
protein update 2009. 6.13
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SSJ127 is cyt
5' end seq. ID SSJ127F
5' end seq. 
>SSJ127F.Seq
CACTTCATATTTATTTTTTTTTTTTTTTTTCATATATATTATTTATTAAAAGATGACAGA
AAAGCAATTAAGTTGTTGTCTCGATTTAATGAGACGTTTACCACCATCTCAAATTGAAGA
TAATTTAGCAGGTCTCCTTGATCTTGTCCCAGATCTCACTGAAGACCTTTTATCATCAAT
TGATCAACCACTCAAGGTTGCTTATGATGCAGTTTCAAAAAAAGATTATTTATTATGTGA
TTACAATAGAGATGCTGATTCATACAGATCACCATGGTCAAACAAATATGATCCACCATT
AAGTGGTGCATGTTATCCATCATCAAAATTAAGAGATATTGAAGTTCAAGCTAATGAAAT
TTTTGAAATTTATCTTAACTTATATTTTGAAGGTGGTGTTTCATCTGTTTATTGTTGGGA
TCTCGATGATAATTTCGCTGCTGTAGTTTTAATGAAAAAAACTCAAGATCAATCTAAAAA
AGGTCAACCAATGAGAGGTACTTGGGATTCAATTCATGTTGTTGAAGTTAAACTT-----
-----
Length of 5' end seq. 535
3' end seq. ID SSJ127Z
3' end seq. 
>SSJ127Z.Seq
----------TGCCGACCCCCCACCCCTCACCATCCACCTCAACCGTGGGGAAGGGAGAT
GGGGGGTTATACGCGGAATGTGCGACGGTACTAACACCCTTTTCAATGCAATTGTCAATA
AAGAGTTGAGAAAGTTCACTTTTATCCTCATTGTATAGACTAGTTCTAGTATGCGACGAG
CGGNCCGACCACCGACGGTGGCNCTACCAATT
Length of 3' end seq. 202
Connected seq. ID SSJ127P
Connected seq. 
>SSJ127P.Seq
CACTTCATATTTATTTTTTTTTTTTTTTTTCATATATATTATTTATTAAAAGATGACAGA
AAAGCAATTAAGTTGTTGTCTCGATTTAATGAGACGTTTACCACCATCTCAAATTGAAGA
TAATTTAGCAGGTCTCCTTGATCTTGTCCCAGATCTCACTGAAGACCTTTTATCATCAAT
TGATCAACCACTCAAGGTTGCTTATGATGCAGTTTCAAAAAAAGATTATTTATTATGTGA
TTACAATAGAGATGCTGATTCATACAGATCACCATGGTCAAACAAATATGATCCACCATT
AAGTGGTGCATGTTATCCATCATCAAAATTAAGAGATATTGAAGTTCAAGCTAATGAAAT
TTTTGAAATTTATCTTAACTTATATTTTGAAGGTGGTGTTTCATCTGTTTATTGTTGGGA
TCTCGATGATAATTTCGCTGCTGTAGTTTTAATGAAAAAAACTCAAGATCAATCTAAAAA
AGGTCAACCAATGAGAGGTACTTGGGATTCAATTCATGTTGTTGAAGTTAAACTT-----
-----TGCCGACCCCCCACCCCTCACCATCCACCTCAACCGTGGGGAAGGGAGATGGGGG
GTTATACGCGGAATGTGCGACGGTACTAACACCCTTTTCAATGCAATTGTCAATAAAGAG
TTGAGAAAGTTCACTTTTATCCTCATTGTATAGACTAGTTCTAGTATGCGACGAGCGGNC
CGACCACCGACGGTGGCNCTACCAATT
Length of connected seq. 737
Full length Seq ID -
Full length Seq. -
Length of full length seq. -