>SSI457 (SSI457Q) /CSM/SS/SSI4-C/SSI457Q.Seq.d/
XXXXXXXXXXCAATTTATGGTCGTTCACCATGGAGAGTTCAACATATGACTTTTAACATC
TGGTATACTACAAATACTACCAATGGTAATGTTTATGCATTTGTATTTGAATTACCAGAT
GATGGTGTTTTAATTTTAAGTGATCCAATTGGTAATAATAAAACAGAAGCAACTTTATTA
GGTTTAAAAGGTGAAAAAGGTGTTGAAGTATCATTACCAATTGAATCAACAAAACCTGGT
ATTACACTAAACATTCCAATGGTTGCACCACAAGATTATCCACCATATGTTTATGTTTTT
AGATTAACTGATGTAGAATAAAAAAAATAAAAAAATAAAAAAATAAAAAAAAAAAATTAA
AAATAAAATAAATAAATAAATAAATGTAAAAAAAAAAAT

---IYGRSPWRVQHMTFNIWYTTNTTNGNVYAFVFELPDDGVLILSDPIGNNKTEATLLG
LKGEKGVEVSLPIESTKPGITLNIPMVAPQDYPPYVYVFRLTDVE*kk*knkkikkkn*k
*nk*inkckkkn

Frame A:
---nlwsftmesstydf*hlvyykyyqw*clcici*itr*wcfnfk*snw***nrsnfir
fkr*krc*siitn*inktwyytkhsngcttrlsticlcf*in*crikkikk*knkkkklk
ik*ink*m*kkk


Frame B:
---IYGRSPWRVQHMTFNIWYTTNTTNGNVYAFVFELPDDGVLILSDPIGNNKTEATLLG
LKGEKGVEVSLPIESTKPGITLNIPMVAPQDYPPYVYVFRLTDVE*kk*knkkikkkn*k
*nk*inkckkkn


Frame C:
---fmvvhhgefni*lltsgilqilpmvmfmhlylnyqmmvf*f*viqlviikqkqly*v
*kvkkvlkyhyqlnqqnlvlh*tfqwlhhkiihhmfmfld*lm*nkknkkikk*kkkikn
kink*invkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI457 (SSI457Q) /CSM/SS/SSI4-C/SSI457Q.Seq.d/                        613   e-175
SSJ396 (SSJ396Q) /CSM/SS/SSJ3-D/SSJ396Q.Seq.d/                        597   e-170
SSI823 (SSI823Q) /CSM/SS/SSI8-A/SSI823Q.Seq.d/                        597   e-170
SLG555 (SLG555Q) /CSM/SL/SLG5-C/SLG555Q.Seq.d/                        597   e-170
SLC793 (SLC793Q) /CSM/SL/SLC7-D/SLC793Q.Seq.d/                        597   e-170
SFE510 (SFE510Q) /CSM/SF/SFE5-A/SFE510Q.Seq.d/                        597   e-170
SLI638 (SLI638Q) /CSM/SL/SLI6-B/SLI638Q.Seq.d/                        589   e-167
SFD201 (SFD201Q) /CSM/SF/SFD2-A/SFD201Q.Seq.d/                        575   e-163
SFI377 (SFI377Q) /CSM/SF/SFI3-D/SFI377Q.Seq.d/                        569   e-161
SFE364 (SFE364Q) /CSM/SF/SFE3-C/SFE364Q.Seq.d/                        567   e-161

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y07497|Y07497.1 Dictyostelium discoideum putative alpha-L-fucosidase gene.   597  e-167  1
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   597  e-167  1
BH719547|BH719547.1 BOMMS93TR BO_2_3_KB Brassica oleracea genomic clone BOMMS93, DNA sequence.    44  1.3  1
AL439037|AL439037.1 T7 end of clone BD0AA002A09 of library BD0AA from strain CBS 94 of  Candida tropicalis.    44  1.3  1
CB955735|CB955735.1 AGENCOURT_13777727 NIH_MGC_184 Homo sapiens cDNA clone IMAGE:30350239 5', mRNA sequence.    30  1.6  3
AE010570|AE010570.1 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, section 112 of 197 of the complete genome.    34  2.3  2
AC108682|AC108682.2 Homo sapiens Xp BAC RP11-325L9 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    34  2.6  3
BB028965|BB028965.1 Mus musculus 8 days embryo cDNA, RIKEN full-length enriched library, clone:5730509C19, 3' end partial sequence, similar to ddbj:AF108653 Rattus norvegicus nucleic acid binding factor pRM10 (pRM10) mRNA.    38  4.3  2
AC120070|AC120070.5 Rattus norvegicus clone CH230-481C18, *** SEQUENCING IN PROGRESS ***, 19 unordered pieces.    42  5.0  1
AP001622|AP001622.1 Homo sapiens genomic DNA, chromosome 21, clone:KB1430A10, MX1-D21S171 region.    42  5.0  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P10901) RecName: Full=Alpha-L-fucosidase;          EC=3.2.1.51;...   209   2e-53
BT075265_1(BT075265|pid:none) Osmerus mordax clone omor-rgc-505-...    59   4e-08
BC133212_1(BC133212|pid:none) Xenopus laevis hypothetical protei...    57   1e-07
BC070977_1(BC070977|pid:none) Xenopus laevis hypothetical protei...    57   1e-07
HWHUFA(A33427;A00901;A61016;I54348;I37394;I84457) alpha-L-fucosi...    56   3e-07
AK314649_1(AK314649|pid:none) Homo sapiens cDNA, FLJ95493, highl...    56   3e-07
AK301302_1(AK301302|pid:none) Homo sapiens cDNA FLJ52780 complet...    56   3e-07
AK092914_1(AK092914|pid:none) Homo sapiens cDNA FLJ35595 fis, cl...    56   3e-07
AB171645_1(AB171645|pid:none) Macaca fascicularis brain cDNA clo...    55   6e-07
BC074217_1(BC074217|pid:none) Xenopus laevis cDNA clone MGC:8339...    54   1e-06

 psg: 0.85  gvh: 0.31  alm: 0.41  top: 0.50  tms: 0.00  mit: 0.46  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: mitochondrial
	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SSI457 is mit

>SSI457Z.Seq
----------CAATTTATGGTCGTTCACCATGGAGAGTTCAACATATGACTTTTAACATC
TGGTATACTACAAATACTACCAATGGTAATGTTTATGCATTTGTATTTGAATTACCAGAT
GATGGTGTTTTAATTTTAAGTGATCCAATTGGTAATAATAAAACAGAAGCAACTTTATTA
GGTTTAAAAGGTGAAAAAGGTGTTGAAGTATCATTACCAATTGAATCAACAAAACCTGGT
ATTACACTAAACATTCCAATGGTTGCACCACAAGATTATCCACCATATGTTTATGTTTTT
AGATTAACTGATGTAGAATAAAAAAAATAAAAAAATAAAAAAATAAAAAAAAAAAATTAA
AAATAAAATAAATAAATAAATAAATGTAAAAAAAAAAAT