>SSI295 (SSI295Q) /CSM/SS/SSI2-D/SSI295Q.Seq.d/
GAAGATAAAAAAAGAAATGATAAATATATTATTACTCGGTTCTTGCTTTGTTGCAAAGCC
AGCACTTGATTACCTCTTAAAAAGAGAAGATTATTTTGTAACAATTGTTAGTTTATTCCA
AAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATTCAAAACAATTCAATT
AGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAAATCTGATTGTGTAAT
TAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATGTATTAAACATAAAAC
ACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATTATCAGAAGAAGCAAA
GGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGGTATTGATCATATGTC
ATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGTCACATCATTCGTTTC
ATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATTTGGATACAAATTTTC
ATGGTCACCAAGAGGTGTACTATCATCAGCAACTCTTTCCGCCAATTTCCTTXXXXXXXX
XXCTCGAAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATA
ACTTTGTCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATG
GTATTCCAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAA
CTGAACCCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCANTNACTCCTG
AATTTTATTTACCAATTTTAGAGAAATTAAAATCTGAAAATATTGAAATGATTGAAACTA
AACAAGAAATTAAAAAATAAAATAAAATAATATTAAATTTTTTAAAAAAAAAAAAAAAAA
AAAAAAAAAAA

KIKKEMINILLLGSCFVAKPALDYLLKREDYFVTIVSLFQNELDSITKGHDTSKFKTIQL
DVMNKLNELEEYFPKSDCVISLIPATLHSTVAKLCIKHKTHLVTASYISDDMKALSEEAK
EAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWCGALPSTECADNPFGYKFS
WSPRGVLSSATLSANFL---

---LEKKLSYKAGERDVVVLEHNFVVQYADRTEKEVSSLICYGIPNGSSATSLTVGVPVG
IATEPIADGKTTTRGVVGPXTPEFYLPILEKLKSENIEMIETKQEIKK*nkiilnflkkk
kkkkkk

Frame A:
edkkrndkyiitrfllcckast*lplkkrrlfcnnc*fipk*irlnykrsryikiqnnsi
rcye*ie*irrifpki*lcn*fnssnitfnsckimy*t*ntfsysklyfr*yesiirrsk
ggrcfiie*tws*sry*syvinedy*sc*gkww*ghiirfmvwcatinrmc*qsiwiqif
mvtkrctiisnsfrqfp---

---LEKKLSYKAGERDVVVLEHNFVVQYADRTEKEVSSLICYGIPNGSSATSLTVGVPVG
IATEPIADGKTTTRGVVGPXTPEFYLPILEKLKSENIEMIETKQEIKK*nkiilnflkkk
kkkkkk

Frame B:
KIKKEMINILLLGSCFVAKPALDYLLKREDYFVTIVSLFQNELDSITKGHDTSKFKTIQL
DVMNKLNELEEYFPKSDCVISLIPATLHSTVAKLCIKHKTHLVTASYISDDMKALSEEAK
EAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWCGALPSTECADNPFGYKFS
WSPRGVLSSATLSANFL---

---skrnsltklvnvm*lslnitlssntlivqkkkfhhsfvmvfqmvvlqlhsllvfqlv
sqlnpslmvklqlevllvxxllnfiyqf*rn*nlkilk*lklnkklknkik*y*if*kkk
kkkkk

Frame C:
r*kkk**iyyysvlallqsqhlits*kekiil*qllvyskmn*tqlqkvtihqnskqfn*
ml*in*mn*knisqnliv*lv*fqqhyiqqlqnyvlnikhi*lqqvifqmi*khyqkkqr
rqvfyy*mnlvliqvliichq*rllimlrkmvvrshhsfhgvvryhqqnvltihldtnfh
ghqevyyhqqlfppis---

---rketllqsw*t*cscp*t*lcrpir*syrkrsfithllwyskw*fcnfthcwcsswy
rn*thr*w*nyn*rccwsxxs*ilftnfreiki*ky*nd*n*trn*kik*nnikffkkkk
kkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI295 (SSI295Q) /CSM/SS/SSI2-D/SSI295Q.Seq.d/                       1639   0.0
VFM605 (VFM605Q) /CSM/VF/VFM6-A/VFM605Q.Seq.d/                       1132   0.0
VFC723 (VFC723Q) /CSM/VF/VFC7-A/VFC723Q.Seq.d/                       1132   0.0
SFJ812 (SFJ812Q) /CSM/SF/SFJ8-A/SFJ812Q.Seq.d/                       1132   0.0
SFE805 (SFE805Q) /CSM/SF/SFE8-A/SFE805Q.Seq.d/                       1132   0.0
CFE334 (CFE334Q) /CSM/CF/CFE3-B/CFE334Q.Seq.d/                       1100   0.0
VFA508 (VFA508Q) /CSM/VF/VFA5-A/VFA508Q.Seq.d/                       1086   0.0
VFE172 (VFE172Q) /CSM/VF/VFE1-C/VFE172Q.Seq.d/                       1084   0.0
VFM495 (VFM495Q) /CSM/VF/VFM4-D/VFM495Q.Seq.d/                       1082   0.0
AFK803 (AFK803Q) /CSM/AF/AFK8-A/AFK803Q.Seq.d/                       1059   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AZ928392|AZ928392.1 479.dif09b09.s1 Saccharomyces kluyveri Saccharomyces kluyveri genomic clone 479.dif09b09.s1, DNA sequence.    40  0.001  2
AC138524|AC138524.1 Homo sapiens chromosome 5 clone XXyac-4X104D9, WORKING DRAFT SEQUENCE, 308 unordered pieces.    52  0.013  1
AX594978|AX594978.1 Sequence 632 from Patent EP1258494.           52  0.013  1
X77363|X77363.1 S.cerevisiae LYS9 gene.                           52  0.013  1
Z71665|Z71665.1 S.cerevisiae chromosome XIV reading frame ORF YNR050c.    52  0.013  1
BW208937|BW208937.1 Ciona intestinalis cDNA, clone:cieg059i20, 5' end, single read.    36  0.074  2
BW266970|BW266970.1 Ciona intestinalis cDNA, clone:cign045o17, 5' end, single read.    36  0.075  2
AL499626|AL499626.5 Human DNA sequence from clone RP3-381A2 on chromosome 6.    48  0.20  1
AC105463|AC105463.5 Rattus norvegicus clone CH230-204J5, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    34  0.51  7
AL394098|AL394098.1 T3 end of clone AR0AA015A07 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    46  0.81  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NG9) RecName: Full=Probable saccharopine dehydrogenase [NADP...   380   e-104
BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...   168   3e-40
FM992690_618(FM992690|pid:none) Candida dubliniensis CD36 chromo...   167   5e-40
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   166   8e-40
CR382130_918(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   165   2e-39
AM989986_8(AM989986|pid:none) Zygosaccharomyces rouxii strain CB...   165   2e-39
(O59711) RecName: Full=Saccharopine dehydrogenase [NADP+, L-glut...   162   1e-38
(A2VCW9) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   162   2e-38
AY751465_1(AY751465|pid:none) Oncorhynchus mykiss lysine ketoglu...   162   2e-38
AM270078_29(AM270078|pid:none) Aspergillus niger contig An04c017...   161   3e-38

 psg: 0.95  gvh: 0.54  alm: 0.34  top: 0.33  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 24.0 %: extracellular, including cell wall
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for SSI295 is cyt

>SSI295F.Seq
GAAGATAAAAAAAGAAATGATAAATATATTATTACTCGGTTCTTGCTTTGTTGCAAAGCC
AGCACTTGATTACCTCTTAAAAAGAGAAGATTATTTTGTAACAATTGTTAGTTTATTCCA
AAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATTCAAAACAATTCAATT
AGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAAATCTGATTGTGTAAT
TAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATGTATTAAACATAAAAC
ACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATTATCAGAAGAAGCAAA
GGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGGTATTGATCATATGTC
ATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGTCACATCATTCGTTTC
ATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATTTGGATACAAATTTTC
ATGGTCACCAAGAGGTGTACTATCATCAGCAACTCTTTCCGCCAATTTCCTT--------
--

>SSI295Z.Seq
----------CTCGAAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCT
TGAACATAACTTTGTCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCAT
TTGTTATGGTATTCCAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGG
TATCGCAACTGAACCCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCANT
NACTCCTGAATTTTATTTACCAATTTTAGAGAAATTAAAATCTGAAAATATTGAAATGAT
TGAAACTAAACAAGAAATTAAAAAATAAAATAAAATAATATTAAATTTTTTAAAAAAAAA
AAAAAAAAAAAAAAAAAAA

>SSI295P.Seq
GAAGATAAAAAAAGAAATGATAAATATATTATTACTCGGTTCTTGCTTTGTTGCAAAGCC
AGCACTTGATTACCTCTTAAAAAGAGAAGATTATTTTGTAACAATTGTTAGTTTATTCCA
AAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATTCAAAACAATTCAATT
AGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAAATCTGATTGTGTAAT
TAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATGTATTAAACATAAAAC
ACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATTATCAGAAGAAGCAAA
GGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGGTATTGATCATATGTC
ATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGTCACATCATTCGTTTC
ATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATTTGGATACAAATTTTC
ATGGTCACCAAGAGGTGTACTATCATCAGCAACTCTTTCCGCCAATTTCCTT--------
--CTCGAAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATA
ACTTTGTCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATG
GTATTCCAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAA
CTGAACCCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCANTNACTCCTG
AATTTTATTTACCAATTTTAGAGAAATTAAAATCTGAAAATATTGAAATGATTGAAACTA
AACAAGAAATTAAAAAATAAAATAAAATAATATTAAATTTTTTAAAAAAAAAAAAAAAAA
AAAAAAAAAAA