>AFB371 (AFB371Q) /CSM/AF/AFB3-C/AFB371Q.Seq.d/
XXXXXXXXXXTGGTGTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTT
ATCGTTNTCATGGTCGTTATCTTCAAACTTATATGACTCAATATAAAGGTCACTATTTCA
CTGGTGATGGTGTAAAACTTGACTCTGATGGTTATTATTGGATCGAAGGTAGAGTAGATG
ATGTTATCAATGTTAGTGGTCATAGATTAGGTACTGCTGAATTAGAGAGTGCCCTCGTTG
GTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGGTCAAG
GTATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGTTATTA
TGATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGTAATTG
TTATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCTTAGAA
AAATTGGTTGTCATGAATCTTCTGCTGAACAATTAGGTGATATTTCAACTTTAGC

---GVLAISKPWPGIARSVYRXHGRYLQTYMTQYKGHYFTGDGVKLDSDGYYWIEGRVDD
VINVSGHRLGTAELESALVGCSICAEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIM
MLKKEVRNVIGPFATPDVIVITPSLPKTRSGKIMRRILRKIGCHESSAEQLGDISTL

Frame A:
---GVLAISKPWPGIARSVYRXHGRYLQTYMTQYKGHYFTGDGVKLDSDGYYWIEGRVDD
VINVSGHRLGTAELESALVGCSICAEAAVVGYPHDIKGQGILAFCTLKEGYQEDESNVIM
MLKKEVRNVIGPFATPDVIVITPSLPKTRSGKIMRRILRKIGCHESSAEQLGDISTL


Frame B:
---vywqslnlgqvllvqfivxmvvifkli*lnikvtislvmv*nltlmviigskve*mm
lsmlvvid*vlln*rvpslvvqfvqrlpllvilmiskvkvf*hfvhskkvikrmnqmll*
c*kkklem*lvhlqhlm*ll*hlhfqklvvvkl*eefleklvvmnlllnn*vifql*


Frame C:
---cignl*tlarycsfslsxswslssnlydsi*rslfhw*wckt*l*wllldrr*sr*c
yqc*ws*iryc*irecprwlfnlcrgcrcwlss*yqrsryfsilytqrrlsrg*ikcyyd
vkkrs*kcnwsicnt*cncyntftskns*w*nyekns*knwls*ifc*tir*yfnfs

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO771 (VHO771Q) /CSM/VH/VHO7-C/VHO771Q.Seq.d/                        993   0.0
VHO673 (VHO673Q) /CSM/VH/VHO6-D/VHO673Q.Seq.d/                        993   0.0
VHJ869 (VHJ869Q) /CSM/VH/VHJ8-C/VHJ869Q.Seq.d/                        993   0.0
SFF506 (SFF506Q) /CSM/SF/SFF5-A/SFF506Q.Seq.d/                        993   0.0
SFB864 (SFB864Q) /CSM/SF/SFB8-C/SFB864Q.Seq.d/                        993   0.0
CHF658 (CHF658Q) /CSM/CH/CHF6-C/CHF658Q.Seq.d/                        993   0.0
CFJ158 (CFJ158Q) /CSM/CF/CFJ1-C/CFJ158Q.Seq.d/                        993   0.0
AFE757 (AFE757Q) /CSM/AF/AFE7-C/AFE757Q.Seq.d/                        993   0.0
AFB371 (AFB371Q) /CSM/AF/AFB3-C/AFB371Q.Seq.d/                        993   0.0
VHE704 (VHE704Q) /CSM/VH/VHE7-A/VHE704Q.Seq.d/                        981   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   702  0.0  2
AX489103|AX489103.1 Sequence 6403 from Patent WO02053728.         70  2e-20  4
CD978883|CD978883.1 QAF6c12.yg QAF Zea mays cDNA clone QAF6c12, mRNA sequence.    42  2e-14  4
AW037040|AW037040.1 614025A12.y1 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence.    42  7e-14  4
AZ930496|AZ930496.1 474.dhz54h09.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz54h09.s1, DNA sequence.    72  1e-13  2
BI674812|BI674812.1 949067E10.y2 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42  1e-13  4
BI478599|BI478599.1 949067E10.x1 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42  3e-12  4
BI788396|BI788396.1 sag70c05.y1 Gm-c1082 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1082-1666 5' similar to TR:Q9ZR69 Q9ZR69 ACETYL-COA SYNTHETASE ;, mRNA sequence.    50  4e-11  3
AW704225|AW704225.1 sk17d07.y1 Gm-c1028 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1028-2438 5' similar to TR:Q9ZR69 Q9ZR69 ACETYL-COA SYNTHETASE ;, mRNA sequence.    50  5e-11  3
BE493668|BE493668.1 WHE0574_A02_B04ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0574_A02_B04, mRNA sequence.    50  6e-09  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115612_37(AC115612|pid:none) Dictyostelium discoideum chromoso...   354   6e-97
(Q54Z60) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   354   6e-97
(Q6MNF1) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   214   1e-54
CP000927_111(CP000927|pid:none) Caulobacter sp. K31, complete ge...   211   1e-53
CP000158_1385(CP000158|pid:none) Hyphomonas neptunium ATCC 15444...   209   3e-53
DQ782753_1(DQ782753|pid:none) Polytomella parva plastid acetyl C...   209   4e-53
(A1U2S9) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   208   6e-53
CP000157_932(CP000157|pid:none) Erythrobacter litoralis HTCC2594...   208   7e-53
CP000699_3452(CP000699|pid:none) Sphingomonas wittichii RW1, com...   207   2e-52
CP000577_2215(CP000577|pid:none) Rhodobacter sphaeroides ATCC 17...   206   2e-52

 psg: 0.71  gvh: 0.46  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for AFB371 is nuc

>AFB371Z.Seq
----------TGGTGTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTT
ATCGTTNTCATGGTCGTTATCTTCAAACTTATATGACTCAATATAAAGGTCACTATTTCA
CTGGTGATGGTGTAAAACTTGACTCTGATGGTTATTATTGGATCGAAGGTAGAGTAGATG
ATGTTATCAATGTTAGTGGTCATAGATTAGGTACTGCTGAATTAGAGAGTGCCCTCGTTG
GTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTATCCTCATGATATCAAAGGTCAAG
GTATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAAGAGGATGAATCAAATGTTATTA
TGATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCATTTGCAACACCTGATGTAATTG
TTATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAAATTATGAGAAGAATTCTTAGAA
AAATTGGTTGTCATGAATCTTCTGCTGAACAATTAGGTGATATTTCAACTTTAGC