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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSI250
Library SS
(Link to library)
Clone ID SSI250
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U05055-1
Original site URL
Representative seq. ID SSI250Z
(Link to Original site)
Representative DNA sequence 
>SSI250 (SSI250Q) /CSM/SS/SSI2-C/SSI250Q.Seq.d/
XXXXXXXXXXTAAATAAAAATGTCACAAACTGATATCCTCTACGCCTGTGTTAGTTATAA
AGGTGTTTGTTTAGTAGAACATAAAATTGCAAATGGTAATTTTATTGATTTAGCAAGAAG
ACTTATCACAAAGATTCCACCAACAAGTAAAAAGATTTACACATCAGAGAATCATAATTT
TCACTATATTTCAGAGAATGATTTAGCGTTTTTATGTTTATGTCACGAGAAATTAGGAGT
TCAAATACCATCAGAATTTTTATCAGATATTAGACAACAATTCATTCGTTCATATGGACA
ATCATTTTCTCAAAATTCACCAACAGCTACATACGACCCATTCATTAGAGTTTTAGAGGA
ACGTATGAAATATTACTCAAATCCAAAATCGAACAAAATGAATTTAGTTATGGATCAAGT
ATCAGAAGCTAAAGGTGCTTTAACTGATGCTATAGAAAAGACAATTCACAGAGGTGAAAA
GATTGAAATTATTGTAGATAAAACTGAAAGACTTCAATCAGAATCATTTGTTTTTAAAAG
TAACTCTGTTGCTTTAAAAAGAAAACTTTGGTGGCAAAATAAAAAATTAGCAATTGCAAT
TGGTTTAGTTGTTTGTATTTTAATTGCTGTAATTACCCTTGCTCTCTTAAAATATTTCAA
AGTAATTTAAATAAATCCCTATCAAAACTTTTACACTGGAAATCAAA
sequence update 1997.12.31
Translated Amino Acid sequence 
---IKMSQTDILYACVSYKGVCLVEHKIANGNFIDLARRLITKIPPTSKKIYTSENHNFH
YISENDLAFLCLCHEKLGVQIPSEFLSDIRQQFIRSYGQSFSQNSPTATYDPFIRVLEER
MKYYSNPKSNKMNLVMDQVSEAKGALTDAIEKTIHRGEKIEIIVDKTERLQSESFVFKSN
SVALKRKLWWQNKKLAIAIGLVVCILIAVITLALLKYFKVI*inpyqnfytgnq
Translated Amino Acid sequence (All Frames) 
Frame A:
---k*kchklisstpvlvikvfv**niklqmvilli*qedlsqrfhqqvkrfthqriiif
tifqrmi*rfyvyvtrn*efkyhqnfyqildnnsfvhmdnhflkihqqlhtthslef*rn
v*nitqiqnrtk*i*lwikyqklkvl*lml*krqftevkrlkll*iklkdfnqnhlflkv
tlll*kenfggkikn*qlqlv*lfvf*ll*lplls*nisk*fk*ipiktftleik


Frame B:
---nknvtn*yplrlc*l*rclfsrt*nckw*fy*fskktyhkdstnk*kdlhires*fs
lyfre*fsvfmfmsreirssntirifiry*ttihsfiwtiifskftnsyirpih*sfrgt
yeillkskieqnefsygssirs*rcfn*cyrkdnsqr*kd*nycr*n*ktsiriicf*k*
lccfkkktlvak*kisncnwfsclyfnccnypcslkifqsnlnkslskllhwks


Frame C:
---IKMSQTDILYACVSYKGVCLVEHKIANGNFIDLARRLITKIPPTSKKIYTSENHNFH
YISENDLAFLCLCHEKLGVQIPSEFLSDIRQQFIRSYGQSFSQNSPTATYDPFIRVLEER
MKYYSNPKSNKMNLVMDQVSEAKGALTDAIEKTIHRGEKIEIIVDKTERLQSESFVFKSN
SVALKRKLWWQNKKLAIAIGLVVCILIAVITLALLKYFKVI*inpyqnfytgnq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI250 (SSI250Q) /CSM/SS/SSI2-C/SSI250Q.Seq.d/                       1382   0.0
SSE763 (SSE763Q) /CSM/SS/SSE7-C/SSE763Q.Seq.d/                       1292   0.0
SSM672 (SSM672Q) /CSM/SS/SSM6-C/SSM672Q.Seq.d/                       1172   0.0
SSH201 (SSH201Q) /CSM/SS/SSH2-A/SSH201Q.Seq.d/                        860   0.0
SSG758 (SSG758Q) /CSM/SS/SSG7-C/SSG758Q.Seq.d/                        242   6e-63
VSK122 (VSK122Q) /CSM/VS/VSK1-A/VSK122Q.Seq.d/                         38   0.18
VSG133 (VSG133Q) /CSM/VS/VSG1-B/VSG133Q.Seq.d/                         38   0.18
SSL579 (SSL579Q) /CSM/SS/SSL5-D/SSL579Q.Seq.d/                         38   0.18
SSK754 (SSK754Q) /CSM/SS/SSK7-C/SSK754Q.Seq.d/                         38   0.18
VSJ747 (VSJ747Q) /CSM/VS/VSJ7-B/VSJ747Q.Seq.d/                         36   0.70
own update 2002. 6. 6
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AE014017|AE014017.1 Buchnera aphidicola str. Bp (Baizongia pistaciae), section 2 of 2 of the complete genome.    38  0.026  8
AC013720|AC013720.4 Homo sapiens chromosome 6 clone RP11-281L5, WORKING DRAFT SEQUENCE, 28 unordered pieces.    38  0.14  7
AC137969|AC137969.3 Mus musculus clone RP23-267N11, WORKING DRAFT SEQUENCE, 7 unordered pieces.    40  0.17  4
AX348638|AX348638.1 Sequence 96 from Patent WO0202807.            32  0.20  4
AX344245|AX344245.1 Sequence 92 from Patent WO0200926.            32  0.20  4
AX251859|AX251859.1 Sequence 120 from Patent WO0168911.           32  0.20  4
BX322795|BX322795.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-106E12.    38  0.30  8
BU496789|BU496789.1 PfESToab58h05.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    40  0.33  2
AP001700|AP001700.1 Homo sapiens genomic DNA, chromosome 21q, section 44/105.    36  0.35  5
AC010985|AC010985.8 Homo sapiens BAC clone RP11-521O14 from 2, complete sequence.    40  0.37  6
dna update 2003. 7.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NW7) RecName: Full=Vesicle-associated membrane protein 7A;        430   e-119
(Q86AQ7) RecName: Full=Vesicle-associated membrane protein 7B;  ...   139   8e-32
EF147419_1(EF147419|pid:none) Populus trichocarpa clone WS01230_...   134   3e-30
EF144704_1(EF144704|pid:none) Populus trichocarpa clone WS01117_...   133   5e-30
AM467632_1(AM467632|pid:none) Vitis vinifera contig VV78X147447....   133   6e-30
AK060093_1(AK060093|pid:none) Oryza sativa Japonica Group cDNA c...   132   8e-30
EU964925_1(EU964925|pid:none) Zea mays clone 282584 vesicle-asso...   132   8e-30
EF087886_1(EF087886|pid:none) Picea sitchensis clone WS02915_P10...   132   1e-29
(Q9MAS5) RecName: Full=Putative vesicle-associated membrane prot...   132   1e-29
GM754336_1(GM754336|pid:none) Sequence 29 from Patent WO20081168...   131   2e-29
protein update 2009. 6.13
PSORT 

 psg: 0.87  gvh: 0.39  alm: 0.10  top: 0.33  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: plasma membrane
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for SSI250 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SSI250Z
3' end seq. 
>SSI250Z.Seq
----------TAAATAAAAATGTCACAAACTGATATCCTCTACGCCTGTGTTAGTTATAA
AGGTGTTTGTTTAGTAGAACATAAAATTGCAAATGGTAATTTTATTGATTTAGCAAGAAG
ACTTATCACAAAGATTCCACCAACAAGTAAAAAGATTTACACATCAGAGAATCATAATTT
TCACTATATTTCAGAGAATGATTTAGCGTTTTTATGTTTATGTCACGAGAAATTAGGAGT
TCAAATACCATCAGAATTTTTATCAGATATTAGACAACAATTCATTCGTTCATATGGACA
ATCATTTTCTCAAAATTCACCAACAGCTACATACGACCCATTCATTAGAGTTTTAGAGGA
ACGTATGAAATATTACTCAAATCCAAAATCGAACAAAATGAATTTAGTTATGGATCAAGT
ATCAGAAGCTAAAGGTGCTTTAACTGATGCTATAGAAAAGACAATTCACAGAGGTGAAAA
GATTGAAATTATTGTAGATAAAACTGAAAGACTTCAATCAGAATCATTTGTTTTTAAAAG
TAACTCTGTTGCTTTAAAAAGAAAACTTTGGTGGCAAAATAAAAAATTAGCAATTGCAAT
TGGTTTAGTTGTTTGTATTTTAATTGCTGTAATTACCCTTGCTCTCTTAAAATATTTCAA
AGTAATTTAAATAAATCCCTATCAAAACTTTTACACTGGAAATCAAA
Length of 3' end seq. 697
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -