>SSI238 (SSI238Q) /CSM/SS/SSI2-B/SSI238Q.Seq.d/
XXXXXXXXXXTCATTATCACCTGTGTACTATTAGATAATTTATTATTATTTTTCAAATTT
CTTCAATTCTTTGTATTAAATATTACTCAAACATAAAATAAAAATGGCCTCAACCAAAAG
AAGATTAAACAGAGAAGAATCATCTGTAGTTTTAGGTGAAGAACAAGTTGCTGAATTAAA
AGAAGCTTTTGAACTCTTTGATAAAGATAGAACTGGTTTCATTAAAAAGGATGCCTTAAA
AACCACCTGTAAACAATTTGGTGTTTTTGTTATGGAAGATCAATTAGATGCCATGTTTGC
TGAAGCTGATACCACCAAATCTGGTGCTATTGGTTTCCCAGAATTTATGTCAATGATGTC
CCGTCGTATGAAACAAACTTCAAATGAACAAATTTTAATGAACGCTTTTAAAACTTTTGA
TCCAGAAGGTAATGGTTACATCTTAACAAAAGATTTATCTAAAGCCTTAACAACTTTGGG
TGATAAATTAACTGAAGCAGAGTTACAAGAATTGTTATCAATTTCAGAAAACGAACAAAA
GCAAGTTAAATATGACCTTTTCGTTAATACTCTCTTCAGTAAAAAATAAAGTGATTATTA
AATAGTGGAAAAAAAAAAAAAGTATGTTAAAAACGTTTAGGGAGAAGAAATTAAATAAAA
AAAAAAAAAAATATATAAAAAAAACAAAAAAAAAAAAAATCTATATTTAAATAAAACCCT
GAATATGTAAAAAT

---lspvyy*iiyyyfsnffnsly*ILLKHKIKMASTKRRLNREESSVVLGEEQVAELKE
AFELFDKDRTGFIKKDALKTTCKQFGVFVMEDQLDAMFAEADTTKSGAIGFPEFMSMMSR
RMKQTSNEQILMNAFKTFDPEGNGYILTKDLSKALTTLGDKLTEAELQELLSISENEQKQ
VKYDLFVNTLFSKK*sdy*ivekkkkyvknv*geeik*kkkkiykknkkkknlylnktln
m*k

Frame A:
---hyhlctir*fiiifqissilcikyysnik*kwpqpked*teknhl*f*vknklln*k
kllnslikielvslkrmp*kppvnnlvfllwkin*mpcllklippnlvllvsqnlcq*cp
vv*nklqmnkf**tllklliqkvmvts*qkiylkp*qlwvin*lkqsykncyqfqktnks
klnmtfslilssvknkviik*wkkkksmlktfrekklnkkkkkyikktkkkkiyi*ikp*
ickn


Frame B:
---iitcvlldnlllffkflqffvlnitqt*nknglnqkkikqrriicsfr*rtsc*ikr
sf*tl**r*nwfh*kgclknhl*tiwcfcygrsirchvc*s*yhqiwcywfpriyvndvp
syetnfk*tnfnerf*nf*srr*wlhlnkrfi*slnnfg**in*srvtrivinfrkrtka
s*i*pfr*yslq*kik*llnsgkkkkvc*krlgrrn*ikkkkni*kkqkkkksifk*npe
yvk


Frame C:
---lspvyy*iiyyyfsnffnsly*ILLKHKIKMASTKRRLNREESSVVLGEEQVAELKE
AFELFDKDRTGFIKKDALKTTCKQFGVFVMEDQLDAMFAEADTTKSGAIGFPEFMSMMSR
RMKQTSNEQILMNAFKTFDPEGNGYILTKDLSKALTTLGDKLTEAELQELLSISENEQKQ
VKYDLFVNTLFSKK*sdy*ivekkkkyvknv*geeik*kkkkiykknkkkknlylnktln
m*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI238 (SSI238Q) /CSM/SS/SSI2-B/SSI238Q.Seq.d/                       1185   0.0
SSC193 (SSC193Q) /CSM/SS/SSC1-D/SSC193Q.Seq.d/                       1152   0.0
SSJ450 (SSJ450Q) /CSM/SS/SSJ4-C/SSJ450Q.Seq.d/                       1138   0.0
VSI369 (VSI369Q) /CSM/VS/VSI3-C/VSI369Q.Seq.d/                       1100   0.0
SSD351 (SSD351Q) /CSM/SS/SSD3-C/SSD351Q.Seq.d/                       1080   0.0
SSA512 (SSA512Q) /CSM/SS/SSA5-A/SSA512Q.Seq.d/                       1025   0.0
VFJ504 (VFJ504Q) /CSM/VF/VFJ5-A/VFJ504Q.Seq.d/                       1015   0.0
SSG876 (SSG876Q) /CSM/SS/SSG8-D/SSG876Q.Seq.d/                       1003   0.0
SSF736 (SSF736Q) /CSM/SS/SSF7-B/SSF736Q.Seq.d/                        928   0.0
SSB548 (SSB548Q) /CSM/SS/SSB5-B/SSB548Q.Seq.d/                        878   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M25251|M25251.1 D.discoideum regulatory myosin light chain (rmlc) gene, complete cds.  1102  0.0  1
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.   537  0.0  4
A32507|A32507.1 Synthetic N-terminal fusion NDP kinase gene.     214  1e-51  1
M36679|M36679.1 D.discoideum nucleoside diphosphate kinase Gip17 mRNA, 5' end.   214  1e-51  1
M14760|M14760.1 Yeast (S.cerevisiae) CMD1 gene encoding calmodulin, complete cds.    64  3e-06  1
Z35978|Z35978.1 S.cerevisiae chromosome II reading frame ORF YBR109c.    64  3e-06  1
AF081669|AF081669.1 Synthetic construct VU91B calmodulin gene, complete cds.    64  3e-06  1
AX595120|AX595120.1 Sequence 774 from Patent EP1258494.           64  3e-06  1
AF081667|AF081667.1 Saccharomyces cerevisiae calmodulin gene, complete cds.    64  3e-06  1
X78993|X78993.1 S.cerevisiae genomic DNA 70kb region of the right arm of chromosome  II.    64  2e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13833) RecName: Full=Myosin regulatory light chain; AltName: F...   313   3e-84
AC116979_41(AC116979|pid:none) Dictyostelium discoideum chromoso...   309   5e-83
AB076705_1(AB076705|pid:none) Physarum polycephalum RMLC mRNA fo...   167   2e-40
(O97341) RecName: Full=Calmodulin;          Short=CaM;  &Y18166_...   121   2e-26
CR940348_242(CR940348|pid:none) Theileria annulata strain Ankara...   120   4e-26
(P27165) RecName: Full=Calmodulin;          Short=CaM;  &(Q71UH5...   120   6e-26
(Q9D6P8) RecName: Full=Calmodulin-like protein 3;  &AK010118_1(A...   120   6e-26
AB222133_1(AB222133|pid:none) Pan troglodytes verus calm1 mRNA f...   119   1e-25
MCSFCU(JK0011)calmodulin - sea cucumber (Stichopus japonicus)         119   1e-25
(P21251) RecName: Full=Calmodulin;          Short=CaM;                119   1e-25

 psg: 0.51  gvh: 0.29  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.43  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 32.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for SSI238 is mit

>SSI238Z.Seq
----------TCATTATCACCTGTGTACTATTAGATAATTTATTATTATTTTTCAAATTT
CTTCAATTCTTTGTATTAAATATTACTCAAACATAAAATAAAAATGGCCTCAACCAAAAG
AAGATTAAACAGAGAAGAATCATCTGTAGTTTTAGGTGAAGAACAAGTTGCTGAATTAAA
AGAAGCTTTTGAACTCTTTGATAAAGATAGAACTGGTTTCATTAAAAAGGATGCCTTAAA
AACCACCTGTAAACAATTTGGTGTTTTTGTTATGGAAGATCAATTAGATGCCATGTTTGC
TGAAGCTGATACCACCAAATCTGGTGCTATTGGTTTCCCAGAATTTATGTCAATGATGTC
CCGTCGTATGAAACAAACTTCAAATGAACAAATTTTAATGAACGCTTTTAAAACTTTTGA
TCCAGAAGGTAATGGTTACATCTTAACAAAAGATTTATCTAAAGCCTTAACAACTTTGGG
TGATAAATTAACTGAAGCAGAGTTACAAGAATTGTTATCAATTTCAGAAAACGAACAAAA
GCAAGTTAAATATGACCTTTTCGTTAATACTCTCTTCAGTAAAAAATAAAGTGATTATTA
AATAGTGGAAAAAAAAAAAAAGTATGTTAAAAACGTTTAGGGAGAAGAAATTAAATAAAA
AAAAAAAAAAATATATAAAAAAAACAAAAAAAAAAAAAATCTATATTTAAATAAAACCCT
GAATATGTAAAAAT