>SSI234 (SSI234Q) /CSM/SS/SSI2-B/SSI234Q.Seq.d/
GACCCAAACCAACCTTGACATACTTTCCAGTACGTGGAAGAGTTCAATTACCAAGATGTT
TATTAGAATACCTTGGTGAAGAATATAATTTTAACGAGGTTAAAACTATATCTGATGATT
TAAGAAAACAATTATTGTTTAAACAACTACCACTTTATCAAGAAGGAGATTTCAAAATCG
TTCAAACTCCTGCAATAATTGATTACATTTCAGAGAAACATGATTTTCGTGGTAAAACCA
AAGAAGAAAGAGCAAGAGCTCATCAATGTTTAGCAGGTATAATGGAAGTTTTGGACCATT
GTTTGGGATATTCATTTAAAATGGATAAACAACAACAAGAAAAATTCAGAAATGAAAGTC
CAATAAAGAAATTTTTTGAAGGTTTTGAAATGGTACTTTCTCAAAATAAATATTTGGCAT
CTGGTGAAAAAGAAACTTATGTTGATCTTATGGCATTTGTAATGGTTGACTATGTCACTA
ATTTGGGTCTTATGCCAAGTGGTGATTATCCTAAACTAATCTCTTTGAAAAAACATTATG
AATCATCACCACAACTTAAAAAATATTTGGAATCAAGACCACAATCTAATTTCTAGAATA
TTCCTCAATTTTTTATTAATTTTTAATTTTAATTATTAAATAAAAAAAGAATAAAATAAA
ACTAAATAGCATTCAATGATGATGATAATAATAAAAAAAAAAAAAAAA

PKPTLTYFPVRGRVQLPRCLLEYLGEEYNFNEVKTISDDLRKQLLFKQLPLYQEGDFKIV
QTPAIIDYISEKHDFRGKTKEERARAHQCLAGIMEVLDHCLGYSFKMDKQQQEKFRNESP
IKKFFEGFEMVLSQNKYLASGEKETYVDLMAFVMVDYVTNLGLMPSGDYPKLISLKKHYE
SSPQLKKYLESRPQSNF*nipqffinf*f*llnkkrik*n*iafndddnnkkkkk

Frame A:
dpnqp*htfqyveefnyqdvy*ntlvkniiltrlklylmi*ennyclnnyhfikkeisks
fkllq*litfqrnmifvvkpkkkeqelinv*qv*wkfwtivwdihlkwinnnkknsemkv
q*rnflkvlkwyflkiniwhlvkkklmlilwhl*wltmsliwvlcqvviiln*sl*knim
nhhhnlkniwnqdhnlisriflnfllifnfny*ikke*nktk*hsmmmiiikkkkk


Frame B:
tqtnldilsstwkssitkmfiripw*ri*f*rg*nyi**fkktiiv*ttttlsrrrfqnr
snscnn*lhfret*fsw*nqrrksksssmfsryngsfgplfgifi*ng*tttrkiqk*ks
nkeif*rf*ngtfsk*ifgiw*krnlc*sygicng*lch*fgsyakw*ls*tnlfektl*
iittt*kifgiktti*fleyssify*fliliik*kknkiklnsiq******kkkk


Frame C:
PKPTLTYFPVRGRVQLPRCLLEYLGEEYNFNEVKTISDDLRKQLLFKQLPLYQEGDFKIV
QTPAIIDYISEKHDFRGKTKEERARAHQCLAGIMEVLDHCLGYSFKMDKQQQEKFRNESP
IKKFFEGFEMVLSQNKYLASGEKETYVDLMAFVMVDYVTNLGLMPSGDYPKLISLKKHYE
SSPQLKKYLESRPQSNF*nipqffinf*f*llnkkrik*n*iafndddnnkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI234 (SSI234Q) /CSM/SS/SSI2-B/SSI234Q.Seq.d/                       1201   0.0
SSJ566 (SSJ566Q) /CSM/SS/SSJ5-C/SSJ566Q.Seq.d/                       1174   0.0
AHI677 (AHI677Q) /CSM/AH/AHI6-D/AHI677Q.Seq.d/                       1138   0.0
CFI708 (CFI708Q) /CSM/CF/CFI7-A/CFI708Q.Seq.d/                       1134   0.0
SSL157 (SSL157Q) /CSM/SS/SSL1-C/SSL157Q.Seq.d/                       1102   0.0
CHA473 (CHA473Q) /CSM/CH/CHA4-D/CHA473Q.Seq.d/                       1102   0.0
SSE684 (SSE684Q) /CSM/SS/SSE6-D/SSE684Q.Seq.d/                       1100   0.0
SSK439 (SSK439Q) /CSM/SS/SSK4-B/SSK439Q.Seq.d/                       1082   0.0
SSC810 (SSC810Q) /CSM/SS/SSC8-A/SSC810Q.Seq.d/                       1001   0.0
SSH606 (SSH606Q) /CSM/SS/SSH6-A/SSH606Q.Seq.d/                        981   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL844506|AL844506.1 Plasmodium falciparum chromosome  7.          36  0.021  7
AC116855|AC116855.3 Mus musculus clone RP24-402K18, WORKING DRAFT SEQUENCE, 3 unordered pieces.    38  0.051  4
Z69678|Z69678.1 D.schiavazzii satellite DNA (clone bdopvu2).      48  0.15  1
Z69669|Z69669.1 D.schiavazzii satellite DNA (clone vetpvu1).      48  0.15  1
AC000374|AC000374.1 Human cosmid g1980a170, complete sequence.    38  0.17  4
AL109749|AL109749.22 Human DNA sequence from clone RP6-213H19 on chromosome Xq25-26.3.    34  0.21  5
AF020713|AF020713.1 Bacteriophage SPBc2 complete genome.          38  0.28  4
AC137820|AC137820.11 Medicago truncatula clone mth2-15f9, complete sequence.    48  0.36  2
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          36  0.43  8
AC068775|AC068775.52 Homo sapiens 12 BAC RP11-291B21 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38  0.44  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QV7) RecName: Full=Putative glutathione S-transferase alpha-...   388   e-106
(Q55FF3) RecName: Full=Putative glutathione S-transferase alpha-...   125   2e-27
(Q54VI4) RecName: Full=Putative glutathione S-transferase alpha-...   120   3e-26
(Q86AU1) RecName: Full=Putative glutathione S-transferase alpha-...   120   4e-26
(Q556G3) RecName: Full=Putative glutathione S-transferase alpha-...   104   2e-21
FN357554_38(FN357554|pid:none) Schistosoma mansoni genome sequen...    97   4e-19
(P15964) RecName: Full=Glutathione S-transferase class-mu 26 kDa...    96   1e-18
U13853_1(U13853|pid:none) pGEX-4T1 cloning vector, complete sequ...    89   1e-16
U85201_1(U85201|pid:none) Expression vector pGH/F2.2-163, comple...    89   1e-16
FJ526990_1(FJ526990|pid:none) Cloning vector pEG(KT), complete s...    89   1e-16

 psg: 0.54  gvh: 0.30  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for SSI234 is nuc

>SSI234E.Seq
GACCCAAACCAACCTTGACATACTTTCCAGTACGTGGAAGAGTTCAATTACCAAGATGTT
TATTAGAATACCTTGGTGAAGAATATAATTTTAACGAGGTTAAAACTATATCTGATGATT
TAAGAAAACAATTATTGTTTAAACAACTACCACTTTATCAAGAAGGAGATTTCAAAATCG
TTCAAACTCCTGCAATAATTGATTACATTTCAGAGAAACATGATTTTCGTGGTAAAACCA
AAGAAGAAAGAGCAAGAGCTCATCAATGTTTAGCAGGTATAATGGAAGTTTTGGACCATT
GTTTGGGATATTCATTTAAAATGGATAAACAACAACAAGAAAAATTCAGAAATGAAAGTC
CAATAAAGAAATTTTTTGAAGGTTTTGAAATGGTACTTTCTCAAAATAAATATTTGGCAT
CTGGTGAAAAAGAAACTTATGTTGATCTTATGGCATTTGTAATGGTTGACTATGTCACTA
ATTTGGGTCTTATGCCAAGTGGTGATTATCCTAAACTAATCTCTTTGAAAAAACATTATG
AATCATCACCACAACTTAAAAAATATTTGGAATCAAGACCACAATCTAATTTCTAGAATA
TTCCTCAATTTTTTATTAATTTTTAATTTTAATTATTAAATAAAAAAAGAATAAAATAAA
ACTAAATAGCATTCAATGATGATGATAATAATAAAAAAAAAAAAAAAA