>SSI226 (SSI226Q) /CSM/SS/SSI2-B/SSI226Q.Seq.d/
GAAAGACTTCTTTACTCCTCTATCAAGTTATTAGAGGTGGCTTATTAGATATTAATGTTA
AGCTCACAGATCCAAAAGGAAATACAATTTTTGAACGTCTTCATTTTGATACACAAATGA
AAGGTAAACAAAGTTTTACCGCTGGTGAATCAGGTGCATACAAAGTTTGTTTCAATAATG
AAATGTCAAGATTTACCGCCAAAGTTGTCACATTTACATGGGCTTCTGAAGAAGAAGGTG
TCAAAGAAGTTGCCAAAGGTGATTCAATCACTCCAATGGATCAATCAGTTCAAAAGATTG
AAAGAGTTCTTCAATCAGTTATTCATGAACAAAAGAAATTAAGATATAGAGAACAAGCAA
ACAGAGATACCTCTGAAAGTACTAATGCACGTGTTGTATGGTGGACAATCGCTGAGGTTA
TTGTATTAGTTGTAATGGGTGTTGGTCAAATTTGGTATTTAAGAAAATGGTTTGATAATA
AATCAACAGGAAGAGTTTAAAATAATTTTTTAAAAAAAAAGAAATAAAAAAAAAATAAAT
AAACAATTATAATAAATAAAATAATTTTTTATA

KTSLLLYQVIRGGLLDINVKLTDPKGNTIFERLHFDTQMKGKQSFTAGESGAYKVCFNNE
MSRFTAKVVTFTWASEEEGVKEVAKGDSITPMDQSVQKIERVLQSVIHEQKKLRYREQAN
RDTSESTNARVVWWTIAEVIVLVVMGVGQIWYLRKWFDNKSTGRV*nnflkkkk*kknk*
tiiinkiify

Frame A:
erllyssikllevay*ilmlssqiqkeiqflnvfilihk*kvnkvlplvnqvhtkfvsim
kcqdlppklshlhgllkkkvskklpkviqslqwinqfkrlkeffnqlfmnkrn*dienkq
teiplkvlmhvlyggqslrlly*l*wvlvkfgi*engliinqqeefkiif*kkrnkkkin
kql**ik*ffi


Frame B:
kdfftplssy*rwliry*c*ahrskrkynf*tssf*ytner*tkfyrw*irciqslfq**
nvkiyrqschiymgf*rrrcqrscqr*fnhsngsisskd*ksssisys*tkeiki*rtsk
qryl*ky*ctccmvdnr*gyciscngcwsnlvfkkmv***inrkslk*ffkkkeikkk*i
nnynk*nnfl


Frame C:
KTSLLLYQVIRGGLLDINVKLTDPKGNTIFERLHFDTQMKGKQSFTAGESGAYKVCFNNE
MSRFTAKVVTFTWASEEEGVKEVAKGDSITPMDQSVQKIERVLQSVIHEQKKLRYREQAN
RDTSESTNARVVWWTIAEVIVLVVMGVGQIWYLRKWFDNKSTGRV*nnflkkkk*kknk*
tiiinkiify

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA101 (VSA101Q) /CSM/VS/VSA1-A/VSA101Q.Seq.d/                       1013   0.0
SSI226 (SSI226Q) /CSM/SS/SSI2-B/SSI226Q.Seq.d/                       1013   0.0
SSC132 (SSC132Q) /CSM/SS/SSC1-B/SSC132Q.Seq.d/                       1013   0.0
VSF644 (VSF644Q) /CSM/VS/VSF6-B/VSF644Q.Seq.d/                       1007   0.0
VSK458 (VSK458Q) /CSM/VS/VSK4-C/VSK458Q.Seq.d/                        983   0.0
VSJ565 (VSJ565Q) /CSM/VS/VSJ5-C/VSJ565Q.Seq.d/                        894   0.0
VSH869 (VSH869Q) /CSM/VS/VSH8-C/VSH869Q.Seq.d/                        890   0.0
VHN650 (VHN650Q) /CSM/VH/VHN6-C/VHN650Q.Seq.d/                        870   0.0
VSE315 (VSE315Q) /CSM/VS/VSE3-A/VSE315Q.Seq.d/                        339   3e-92
SLE321 (SLE321Q) /CSM/SL/SLE3-A/SLE321Q.Seq.d/                        188   6e-47

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BX004886|BX004886.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-33P4.    58  1e-04  1
BX465186|BX465186.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-106M10.    58  4e-04  4
BX004994|BX004994.6 Zebrafish DNA sequence from clone DKEY-24J15 in linkage group 22.    58  0.002  3
AC126786|AC126786.15 Medicago truncatula clone mth2-8c2, WORKING DRAFT SEQUENCE, 10 unordered pieces.    40  0.002  5
BX294666|BX294666.6 Zebrafish DNA sequence from clone CH211-251M11 in linkage group 10.    50  0.030  1
AX347076|AX347076.1 Sequence 2147 from Patent WO0200928.          50  0.030  1
AC026821|AC026821.2 Homo sapiens chromosome 9 clone RP11-146H11 map 9, WORKING DRAFT SEQUENCE, 37 unordered pieces.    44  0.087  2
AL355499|AL355499.15 Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP11-328K6.    44  0.092  2
AL954695|AL954695.10 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-202N12.    44  0.095  5
AL512373|AL512373.12 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-564O14.    44  0.097  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT046693_1(BT046693|pid:none) Salmo salar clone ssal-eve-549-119...   113   4e-24
(Q769F9) RecName: Full=Transmembrane emp24 domain-containing pro...   111   1e-23
BC050165_1(BC050165|pid:none) Danio rerio transmembrane emp24 do...   111   2e-23
BT078311_1(BT078311|pid:none) Lepeophtheirus salmonis Pacific fo...   110   3e-23
BT077340_1(BT077340|pid:none) Lepeophtheirus salmonis Pacific fo...   108   8e-23
BT075513_1(BT075513|pid:none) Osmerus mordax clone omor-eva-518-...   108   8e-23
(A7SXK3) RecName: Full=Transmembrane emp24 domain-containing pro...   108   1e-22
BT079590_1(BT079590|pid:none) Esox lucius clone eluc-evq-534-324...   108   1e-22
AE014298_657(AE014298|pid:none) Drosophila melanogaster chromoso...   107   2e-22
AE017341_60(AE017341|pid:none) Cryptococcus neoformans var. neof...   107   2e-22

 psg: 0.75  gvh: 0.50  alm: 0.24  top: 0.80  tms: 0.07  mit: 0.28  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.14  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for SSI226 is nuc

>SSI226E.Seq
GAAAGACTTCTTTACTCCTCTATCAAGTTATTAGAGGTGGCTTATTAGATATTAATGTTA
AGCTCACAGATCCAAAAGGAAATACAATTTTTGAACGTCTTCATTTTGATACACAAATGA
AAGGTAAACAAAGTTTTACCGCTGGTGAATCAGGTGCATACAAAGTTTGTTTCAATAATG
AAATGTCAAGATTTACCGCCAAAGTTGTCACATTTACATGGGCTTCTGAAGAAGAAGGTG
TCAAAGAAGTTGCCAAAGGTGATTCAATCACTCCAATGGATCAATCAGTTCAAAAGATTG
AAAGAGTTCTTCAATCAGTTATTCATGAACAAAAGAAATTAAGATATAGAGAACAAGCAA
ACAGAGATACCTCTGAAAGTACTAATGCACGTGTTGTATGGTGGACAATCGCTGAGGTTA
TTGTATTAGTTGTAATGGGTGTTGGTCAAATTTGGTATTTAAGAAAATGGTTTGATAATA
AATCAACAGGAAGAGTTTAAAATAATTTTTTAAAAAAAAAGAAATAAAAAAAAAATAAAT
AAACAATTATAATAAATAAAATAATTTTTTATA