>SSI168 (SSI168Q) /CSM/SS/SSI1-C/SSI168Q.Seq.d/
ATTTATTTAATTGTAAAACTATTTCAAAAATGATGTCATATGGATACCAACAAACACCAA
TTGTTGCACCACCAGCAAAATTCCAAGCTCTTTGGTTTTATTCATTCTATACTCAAATCC
AACACCAACAACTCCATGAGATGTCTGCTTGGTTTAGATCAATTGATAAAGACAGAAGTG
GTTCAATTTCAGCAATGGAATTACAACATTTACATGTTGGTTATGGCCCATTAGGTATTG
AAACAGCTACAAAATTAATTAGAGTATTTGATGTTGATAAGAGTGGTCAAATTGATTTTT
ATGAATATGCTGCATTGCACTGAGAGATCCCCACATAATTTCCCCAAAGCGTAXXXXXXX
XXXGTGCATTGCATCAATTTATTAATATTTTATATGCCAACTTTTTAGCCAATGACCGTA
ATCGTAGTGGTACAATTGACGCTCAAGAAATTCACAGAGCATTAGGTACATCTGGATTTA
ATCTTCCATTTAACACTGTTAATTTACTTTTCTTAAAAGCCTCACCACGTGGTTATGGTT
TAAAGTTCAGCGACTTCCTTGGATTATGCGCATCCATTGCCATCGCCAGATCATTATTTG
AAGCTCATGATAGAATGAGAACTGGTGTTGCTCATTTGAATGTTAATCACATTTATGATA
TTATTAGCTTAGTTTAAATTAAACTTTTTATAATTGTATTTGATAATTTCTCTATTAAAA
ATAAAAATTAAACAAATATTAATTTGTATTGTAAACCATAAAAAAA

iylivklfqk*chmdtnkhqllhhqqnsklfgfihsilksntnnsmrcllgldqliktev
vqfqqwnyniymlvmah*vlkqlqn*LEYLMLIRVVKLIFMNMLHCTERSPHNFPKA---

---ALHQFINILYANFLANDRNRSGTIDAQEIHRALGTSGFNLPFNTVNLLFLKASPRGY
GLKFSDFLGLCASIAIARSLFEAHDRMRTGVAHLNVNHIYDIISLV*iklfiivfdnfsi
knkn*tninlyckp*k

Frame A:
iylivklfqk*chmdtnkhqllhhqqnsklfgfihsilksntnnsmrcllgldqliktev
vqfqqwnyniymlvmah*vlkqlqn*LEYLMLIRVVKLIFMNMLHCTERSPHNFPKA---

---vhcinllifymptf*pmtvivvvqltlkkfteh*vhldlifhltlliyfs*kphhvv
mv*ssatsldyahplpspdhylklmie*elvlli*mlitfmilla*fklnfl*lyliisl
lkikikqilicivnhkk

Frame B:
fi*l*nyfkndviwiptntnccttskipsslvlfilysnptpttp*dvclv*in**rqkw
fnfsngittftcwlwpiry*nsykin*si*c**ewsn*fl*iccialrdphiispkr---

---ciasiy*yficqlfsq*p*s*wyn*rsrnsqsiryiwi*ssi*hc*ftflkslttwl
wfkvqrlpwimrihchrqiii*ss**nenwccsfec*shl*yy*lsln*tfynci**fly
*k*klnky*fvl*tikk

Frame C:
lfncktiskmmsygyqqtpivappakfqalwfysfytqiqhqqlhemsawfrsidkdrsg
sisamelqhlhvgygplgietatklirvfdvdksgqidfyeyaalh*eipt*fpqsv---

---ALHQFINILYANFLANDRNRSGTIDAQEIHRALGTSGFNLPFNTVNLLFLKASPRGY
GLKFSDFLGLCASIAIARSLFEAHDRMRTGVAHLNVNHIYDIISLV*iklfiivfdnfsi
knkn*tninlyckp*k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSI168 (SSI168Q) /CSM/SS/SSI1-C/SSI168Q.Seq.d/                       1352   0.0
SSH619 (SSH619Q) /CSM/SS/SSH6-A/SSH619Q.Seq.d/                        690   0.0
SSG888 (SSG888Q) /CSM/SS/SSG8-D/SSG888Q.Seq.d/                        690   0.0
SSG731 (SSG731Q) /CSM/SS/SSG7-B/SSG731Q.Seq.d/                        690   0.0
SSG263 (SSG263Q) /CSM/SS/SSG2-C/SSG263Q.Seq.d/                        690   0.0
SSA625 (SSA625Q) /CSM/SS/SSA6-B/SSA625Q.Seq.d/                        670   0.0
VSD549 (VSD549Q) /CSM/VS/VSD5-C/VSD549Q.Seq.d/                        632   e-180
SSB189 (SSB189Q) /CSM/SS/SSB1-D/SSB189Q.Seq.d/                        547   e-155
SSH795 (SSH795Q) /CSM/SS/SSH7-D/SSH795Q.Seq.d/                        539   e-152
SSJ228 (SSJ228Q) /CSM/SS/SSJ2-B/SSJ228Q.Seq.d/                        494   e-138

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF358911|AF358911.1 Dictyostelium discoideum apoptosis-linked gene 2 (alg2A) mRNA, complete cds.   690  0.0  2
AB056713|AB056713.1 Dictyostelium discoideum DdPEF-1 mRNA for penta-EF-hand Ca2+-binding protein, complete cds.   670  0.0  2
AF358913|AF358913.1 Dictyostelium discoideum apoptosis-linked gene 2 (alg2A) gene, complete cds.   494  0.0  5
AY044085|AY044085.1 Dictyostelium discoideum Rho GDP-dissociation inhibitor (rdiA) gene, complete cds; and calcium-binding-like protein gene, partial cds.   523  e-144  1
AF358912|AF358912.1 Dictyostelium discoideum apoptosis-linked gene 2 (alg2B) mRNA, complete cds.    82  1e-13  2
AB056714|AB056714.1 Dictyostelium discoideum DdPEF-2 mRNA for penta-EF-hand Ca2+-binding protein, complete cds.    82  1e-13  2
BU947687|BU947687.1 io56a01.x1 Human insulinoma Homo sapiens cDNA clone IMAGE:6130081 3' similar to TR:Q9SFT5 Q9SFT5 T2E6.2. ;, mRNA sequence.    78  2e-10  1
BZ212379|BZ212379.1 CH230-380C19.TJ CHORI-230 Segment 2 Rattus norvegicus genomic clone CH230-380C19, DNA sequence.    70  4e-08  1
BM768100|BM768100.1 K-EST0051003 S6SNU620s1 Homo sapiens cDNA clone S6SNU620s1-12-G07 5', mRNA sequence.    70  4e-08  1
AC120888|AC120888.1 Oryza sativa (japonica cultivar-group) chromosome 11 clone P0485F09, *** SEQUENCING IN PROGRESS ***, 7 ordered pieces.    70  4e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q95YL4) RecName: Full=Penta-EF hand domain-containing protein 2...   248   1e-64
AY044085_2(AY044085|pid:none) Dictyostelium discoideum Rho GDP-d...   152   8e-36
AF291721_1(AF291721|pid:none) Entamoeba histolytica URE3-BP sequ...    85   2e-15
AF082530_1(AF082530|pid:none) Entamoeba histolytica Grainin 2 (g...    79   1e-13
AF085196_1(AF085196|pid:none) Entamoeba histolytica Grainin 1 (g...    76   1e-12
CT005252_146(CT005252|pid:none) Leishmania major strain Friedlin...    75   2e-12
AB002727_1(AB002727|pid:none) Entamoeba histolytica mRNA for cal...    75   2e-12
AE013599_1152(AE013599|pid:none) Drosophila melanogaster chromos...    72   2e-11
(Q9UBV8) RecName: Full=Peflin; AltName: Full=PEF protein with a ...    70   5e-11
(Q5PQ53) RecName: Full=Peflin; AltName: Full=PEF protein with a ...    70   5e-11

 psg: 0.87  gvh: 0.37  alm: 0.34  top: 0.63  tms: 0.00  mit: 0.25  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for SSI168 is cyt

>SSI168F.Seq
ATTTATTTAATTGTAAAACTATTTCAAAAATGATGTCATATGGATACCAACAAACACCAA
TTGTTGCACCACCAGCAAAATTCCAAGCTCTTTGGTTTTATTCATTCTATACTCAAATCC
AACACCAACAACTCCATGAGATGTCTGCTTGGTTTAGATCAATTGATAAAGACAGAAGTG
GTTCAATTTCAGCAATGGAATTACAACATTTACATGTTGGTTATGGCCCATTAGGTATTG
AAACAGCTACAAAATTAATTAGAGTATTTGATGTTGATAAGAGTGGTCAAATTGATTTTT
ATGAATATGCTGCATTGCACTGAGAGATCCCCACATAATTTCCCCAAAGCGTA-------
---

>SSI168Z.Seq
----------GTGCATTGCATCAATTTATTAATATTTTATATGCCAACTTTTTAGCCAAT
GACCGTAATCGTAGTGGTACAATTGACGCTCAAGAAATTCACAGAGCATTAGGTACATCT
GGATTTAATCTTCCATTTAACACTGTTAATTTACTTTTCTTAAAAGCCTCACCACGTGGT
TATGGTTTAAAGTTCAGCGACTTCCTTGGATTATGCGCATCCATTGCCATCGCCAGATCA
TTATTTGAAGCTCATGATAGAATGAGAACTGGTGTTGCTCATTTGAATGTTAATCACATT
TATGATATTATTAGCTTAGTTTAAATTAAACTTTTTATAATTGTATTTGATAATTTCTCT
ATTAAAAATAAAAATTAAACAAATATTAATTTGTATTGTAAACCATAAAAAAA

>SSI168P.Seq
ATTTATTTAATTGTAAAACTATTTCAAAAATGATGTCATATGGATACCAACAAACACCAA
TTGTTGCACCACCAGCAAAATTCCAAGCTCTTTGGTTTTATTCATTCTATACTCAAATCC
AACACCAACAACTCCATGAGATGTCTGCTTGGTTTAGATCAATTGATAAAGACAGAAGTG
GTTCAATTTCAGCAATGGAATTACAACATTTACATGTTGGTTATGGCCCATTAGGTATTG
AAACAGCTACAAAATTAATTAGAGTATTTGATGTTGATAAGAGTGGTCAAATTGATTTTT
ATGAATATGCTGCATTGCACTGAGAGATCCCCACATAATTTCCCCAAAGCGTA-------
---GTGCATTGCATCAATTTATTAATATTTTATATGCCAACTTTTTAGCCAATGACCGTA
ATCGTAGTGGTACAATTGACGCTCAAGAAATTCACAGAGCATTAGGTACATCTGGATTTA
ATCTTCCATTTAACACTGTTAATTTACTTTTCTTAAAAGCCTCACCACGTGGTTATGGTT
TAAAGTTCAGCGACTTCCTTGGATTATGCGCATCCATTGCCATCGCCAGATCATTATTTG
AAGCTCATGATAGAATGAGAACTGGTGTTGCTCATTTGAATGTTAATCACATTTATGATA
TTATTAGCTTAGTTTAAATTAAACTTTTTATAATTGTATTTGATAATTTCTCTATTAAAA
ATAAAAATTAAACAAATATTAATTTGTATTGTAAACCATAAAAAAA