>SSH518 (SSH518Q) /CSM/SS/SSH5-A/SSH518Q.Seq.d/
AAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACC
AAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGG
GTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTG
TTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCA
AATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAANCCATAACTATGCTGGTT
TAACTTTCCGTAAATTTTAATTTTACTCATAATATGTGAAACAAAACTAAAAAAAAAAAA
AGAATAAAAAAAATAAAAAAAATAAAAAAGCAAAAAAAAAAAAAACACATTGGTAAATAT
TGGTTATTGAGAAAAATAAATATAAATCTTTAAACA

RIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVV
GLGTGQIKTGAPCRSERLAKYNQLVRINEELGEXHNYAGLTFRKF*fys*yvkqn*kkkk
nkknkknkkakkkkthw*ilvieknkyksln

Frame A:
kelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswlaivqvklkipslli*l
lv*vlvklklvlhvdqkd*pntinlleltks*vkxitmlv*lsvnfnfthnm*nktkkkk
rikkikkikkqkkkkhigkywllrkininl*t


Frame B:
kn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*psfr*n*rylhc*fsc
wfrywsn*nwcsm*irkisqiqstc*n*rrvr*xp*lcwfnfp*ililliicetklkkkk
e*kk*kk*kskkkkntlvnigy*ek*i*ifk


Frame C:
RIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVV
GLGTGQIKTGAPCRSERLAKYNQLVRINEELGEXHNYAGLTFRKF*fys*yvkqn*kkkk
nkknkknkkakkkkthw*ilvieknkyksln

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI435 (VSI435Q) /CSM/VS/VSI4-B/VSI435Q.Seq.d/                        672   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                        672   0.0
SSM479 (SSM479Q) /CSM/SS/SSM4-D/SSM479Q.Seq.d/                        672   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                        672   0.0
SSH518 (SSH518Q) /CSM/SS/SSH5-A/SSH518Q.Seq.d/                        672   0.0
SLF154 (SLF154Q) /CSM/SL/SLF1-C/SLF154Q.Seq.d/                        672   0.0
SLE731 (SLE731Q) /CSM/SL/SLE7-B/SLE731Q.Seq.d/                        672   0.0
SLC403 (SLC403Q) /CSM/SL/SLC4-A/SLC403Q.Seq.d/                        672   0.0
SLC241 (SLC241Q) /CSM/SL/SLC2-B/SLC241Q.Seq.d/                        672   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                        672   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL407574|AL407574.1 T7 end of clone AV0AA003B04 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.   123  7e-27  2
BI073826|BI073826.1 kt35e07.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:ENO_CAEEL Q27527 ENOLASE ;, mRNA sequence.    92  5e-26  2
X80474|X80474.1 N.frontalis enolase gene.                         58  1e-24  3
BM395511|BM395511.1 50072-2-9-E10.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  8e-24  1
BM393885|BM393885.1 50072-2-11-F10.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  8e-24  1
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  8e-24  1
BM393788|BM393788.1 50072-2-11-B02.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  8e-24  1
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          60  6e-23  4
AZ932132|AZ932132.1 474.dhz95b01.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz95b01.s1, DNA sequence.   115  5e-22  1
BM394042|BM394042.1 50072-2-12-F06.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   113  2e-21  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   207   9e-53
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   162   4e-39
CR940353_640(CR940353|pid:none) Theileria annulata strain Ankara...   161   6e-39
AY725787_1(AY725787|pid:none) Oncometopia nigricans enolase mRNA...   159   2e-38
(Q9LEJ0) RecName: Full=Enolase 1;          EC=4.2.1.11; AltName:...   159   2e-38
EF121427_1(EF121427|pid:none) Bombyx mori enolase mRNA, complete...   159   3e-38
Z68318_3(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   159   3e-38
Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   159   3e-38
(Q27527) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   159   3e-38
EF064247_1(EF064247|pid:none) Blastocladiella emersonii enolase ...   158   5e-38

 psg: 0.75  gvh: 0.47  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SSH518 is cyt

>SSH518E.Seq
AAAGAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACC
AAATTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGG
GTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTG
TTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCA
AATACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAANCCATAACTATGCTGGTT
TAACTTTCCGTAAATTTTAATTTTACTCATAATATGTGAAACAAAACTAAAAAAAAAAAA
AGAATAAAAAAAATAAAAAAAATAAAAAAGCAAAAAAAAAAAAAACACATTGGTAAATAT
TGGTTATTGAGAAAAATAAATATAAATCTTTAAACA