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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL436
Library AF
(Link to library)
Clone ID AFL436
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15310-1
Original site URL
Representative seq. ID AFL436P
(Link to Original site)
Representative DNA sequence 
>AFL436 (AFL436Q) /CSM/AF/AFL4-B/AFL436Q.Seq.d/
CTTAAATNAAAATTTTATACCCCCTCACACTATATATAAATTTACATATATATAAAAAAA
CACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGAACAC
TAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAGATAC
TGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCACCAC
CACCACCACCCCAACTCCAACTCCAACTCCAACACCAACTCCAACAACTACTACCACAAC
AGAAAAAAAAAGTAAAGAAATTGAAATTGTACCAACACCATCAAATGTACCAGCTTCAAT
TTCAGCATCAACTTCAAATGTTGTAATTGCACCAGCTGTTGAAGCACAAATCACTGATGA
CTTTTCAGTTGTCCCAGAGAAAAATAAAAATAAAAAGAAGATCAATTCAACTGCAACTGA
TGGTACCACTACCACCACCAATATACCAAAACCAACACCAGTTAGAGCACCAATTACCAA
AAAAGGTAACACTGCTCCACGTTCTCAATTCCATTTATTAGCACTCGATGAAGXXXXXXX
XXXCCACTACTAAATCAACTCCAACTCCAACACCAACTCCAACTCCAAGCTGCTACTAAA
GTTGTTGAACAACCAAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAAAGAAATT
AAACAAAATAAAAAAGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTTAATTACT
CCAGGTGTTCCATCCGTAGTTTACAAAATCATTTACGTCGCCCTCATTGGTGTTTTATTA
TTTTCATTAGTACCATTATATTACTCTGGTTTAGATAGTATCTACAGTTATGGTGTCATC
GCTTTAGTTTTAGGTCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCCAAGATTA
CAAGCTTCAAAGGAACAAAAATCAAAATCTGGTAACAAAAAATCAACTACTCGTAAAGTT
AAAGCATAAGTCAATCCCATCATCATCCATTTCCAAANATAAANGTAGATAGGCCTTCAA
TCCCATC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
lnxnfipphtiykftyi*KNTNTNKKFKMAGNKKQVKKNTKPIVVDIDDKPLLDTSNLDT
AVQTSASTKKDGKKVTATTTTTTPTPTPTPTPTPTTTTTTEKKSKEIEIVPTPSNVPASI
SASTSNVVIAPAVEAQITDDFSVVPEKNKNKKKINSTATDGTTTTTNIPKPTPVRAPITK
KGNTAPRSQFHLLALDE---

---PLLNQLQLQHQLQLQAATKVVEQPKEKSSPAPVKKEKEIKQNKKESEGFLFSLMESL
ITPGVPSVVYKIIYVALIGVLLFSLVPLYYSGLDSIYSYGVIALVLGLGISLTLFISEIP
RLQASKEQKSKSGNKKSTTRKVKA*vnpiiihfqx*x*iglqsh
Translated Amino Acid sequence (All Frames) 
Frame A:
lkxkfytpshyi*iyiyikkhkhk*ki*ngrkqets*keh*tnrc*y***tiiryfkfry
cssnkcin*krrkksycyhhhhhpnsnsnsntnsnnyyhnrkkk*rn*nctntikctsfn
fsinfkccnctsc*stnh**lfscprek*k*kedqfncn*wyhyhhqytktnts*stnyq
kr*hcstfsipfistr*---

---PLLNQLQLQHQLQLQAATKVVEQPKEKSSPAPVKKEKEIKQNKKESEGFLFSLMESL
ITPGVPSVVYKIIYVALIGVLLFSLVPLYYSGLDSIYSYGVIALVLGLGISLTLFISEIP
RLQASKEQKSKSGNKKSTTRKVKA*vnpiiihfqx*x*iglqsh

Frame B:
lnxnfipphtiykftyi*KNTNTNKKFKMAGNKKQVKKNTKPIVVDIDDKPLLDTSNLDT
AVQTSASTKKDGKKVTATTTTTTPTPTPTPTPTPTTTTTTEKKSKEIEIVPTPSNVPASI
SASTSNVVIAPAVEAQITDDFSVVPEKNKNKKKINSTATDGTTTTTNIPKPTPVRAPITK
KGNTAPRSQFHLLALDE---

---hy*insnsntnsnsklllkllnnqkknhhqhqlkkkkklnkikknlkdfysv*wnl*
llqvfhp*ftksftspslvfyyfh*yhyitlv*ivstvmvssl*f*vlvfl*lyssvkfq
dyklqrnknqnlvtknqllvklkhksipsssiskxkxr*afnpi

Frame C:
*xkilypltlyinlhiykktqtqiknlkwqetrnklkrtlnqsllilminhy*ilqi*il
qfkqvhqlkktekklllppppppqlqlqlqhqlqqllpqqkkkvkklklyqhhqmyqlqf
qhqlqml*lhqllkhkslmtfqlsqrkikikrrsiqlqlmvplpppiyqnqhqlehqlpk
kvtllhvlnsiy*hsmk---

---ttkstptptptptpscy*sc*ttkrkiitsts*krkrn*tk*kri*rifiqfngifn
ysrcsirslqnhlrrphwcfiifistiillwfr*ylqlwchrfsfrswyffnfihq*nsk
itsfkgtkikiw*qkinys*s*sisqshhhpfpxixvdrpsip
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL436 (AFL436Q) /CSM/AF/AFL4-B/AFL436Q.Seq.d/                        686   0.0
SFJ228 (SFJ228Q) /CSM/SF/SFJ2-B/SFJ228Q.Seq.d/                        660   0.0
SFI764 (SFI764Q) /CSM/SF/SFI7-C/SFI764Q.Seq.d/                        660   0.0
SFG629 (SFG629Q) /CSM/SF/SFG6-B/SFG629Q.Seq.d/                        660   0.0
SFE114 (SFE114Q) /CSM/SF/SFE1-A/SFE114Q.Seq.d/                        660   0.0
AFI617 (AFI617Q) /CSM/AF/AFI6-A/AFI617Q.Seq.d/                        660   0.0
SLB638 (SLB638Q) /CSM/SL/SLB6-B/SLB638Q.Seq.d/                        656   0.0
SLE566 (SLE566Q) /CSM/SL/SLE5-C/SLE566Q.Seq.d/                        654   0.0
SLC662 (SLC662Q) /CSM/SL/SLC6-C/SLC662Q.Seq.d/                        654   0.0
SLB648 (SLB648Q) /CSM/SL/SLB6-B/SLB648Q.Seq.d/                        654   0.0
own update 2001.11.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    32   3e-08  14
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    40   1e-06  19
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    48   1e-06  18
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    36   2e-05  15
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    50   2e-05  20
AL451012|AL451012.1 Neurospora crassa DNA linkage group II BAC clone B21O8.    60   8e-05  1
CF829107|CF829107.1 UCRCR01_02K11_r Clementine Mandarin Albedo at Rind Separation cDNA Library Citrus reticulata cDNA clone CR_CEa02K11, mRNA sequence.    54   9e-05  2
CF829109|CF829109.1 UCRCR01_02K12_r Clementine Mandarin Albedo at Rind Separation cDNA Library Citrus reticulata cDNA clone CR_CEa02K12, mRNA sequence.    54   9e-05  2
AC124744|AC124744.2 Mus musculus chromosome 13 clone RP23-223E16, WORKING DRAFT SEQUENCE, 3 unordered pieces.    58   3e-04  1
AF465309|AF465309.1 Dictyostelium discoideum ABC transporter ABCA.7 (abca7) gene, complete cds.    58   3e-04  1
dna update 2004. 8.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SG3) RecName: Full=Uncharacterized transmembrane protein DDB...   234   4e-60
AP009179_1281(AP009179|pid:none) Sulfurovum sp. NBC37-1 genomic ...    39   0.39
AM295250_1660(AM295250|pid:none) Staphylococcus carnosus subsp. ...    37   1.1
AP008934_718(AP008934|pid:none) Staphylococcus saprophyticus sub...    35   4.4
AF411401_1(AF411401|pid:none) Arenaria interpres NADH dehydrogen...    35   4.4
AY074885_13(AY074885|pid:none) Arenaria interpres mitochondrion,...    35   4.4
AL445067_172(AL445067|pid:none) Thermoplasma acidophilum complet...    35   4.4
EU490746_1(EU490746|pid:none) Gomphichis sp. Trujillo 379 hypoth...    34   9.7
EU490788_1(EU490788|pid:none) Stenoptera sp. Trujillo 389 hypoth...    34   9.7
protein update 2009. 4.25
PSORT 

 psg: 0.57  gvh: 0.42  alm: 0.18  top: 0.47  tms: 0.13  mit: 0.30  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 20.0 %: plasma membrane
	 16.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal
	  4.0 %: cytoplasmic

>> prediction for AFL436 is end
5' end seq. ID AFL436F
5' end seq. 
>AFL436F.Seq
CTTAAATNAAAATTTTATACCCCCTCACACTATATATAAATTTACATATATATAAAAAAA
CACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGAACAC
TAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAGATAC
TGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCACCAC
CACCACCACCCCAACTCCAACTCCAACTCCAACACCAACTCCAACAACTACTACCACAAC
AGAAAAAAAAAGTAAAGAAATTGAAATTGTACCAACACCATCAAATGTACCAGCTTCAAT
TTCAGCATCAACTTCAAATGTTGTAATTGCACCAGCTGTTGAAGCACAAATCACTGATGA
CTTTTCAGTTGTCCCAGAGAAAAATAAAAATAAAAAGAAGATCAATTCAACTGCAACTGA
TGGTACCACTACCACCACCAATATACCAAAACCAACACCAGTTAGAGCACCAATTACCAA
AAAAGGTAACACTGCTCCACGTTCTCAATTCCATTTATTAGCACTCGATGAAG-------
---
Length of 5' end seq. 593
3' end seq. ID AFL436Z
3' end seq. 
>AFL436Z.Seq
----------CCACTACTAAATCAACTCCAACTCCAACACCAACTCCAACTCCAAGCTGC
TACTAAAGTTGTTGAACAACCAAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAA
AGAAATTAAACAAAATAAAAAAGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTT
AATTACTCCAGGTGTTCCATCCGTAGTTTACAAAATCATTTACGTCGCCCTCATTGGTGT
TTTATTATTTTCATTAGTACCATTATATTACTCTGGTTTAGATAGTATCTACAGTTATGG
TGTCATCGCTTTAGTTTTAGGTCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCC
AAGATTACAAGCTTCAAAGGAACAAAAATCAAAATCTGGTAACAAAAAATCAACTACTCG
TAAAGTTAAAGCATAAGTCAATCCCATCATCATCCATTTCCAAANATAAANGTAGATAGG
CCTTCAATCCCATC
Length of 3' end seq. 484
Connected seq. ID AFL436P
Connected seq. 
>AFL436P.Seq
CTTAAATNAAAATTTTATACCCCCTCACACTATATATAAATTTACATATATATAAAAAAA
CACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGAACAC
TAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAGATAC
TGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCACCAC
CACCACCACCCCAACTCCAACTCCAACTCCAACACCAACTCCAACAACTACTACCACAAC
AGAAAAAAAAAGTAAAGAAATTGAAATTGTACCAACACCATCAAATGTACCAGCTTCAAT
TTCAGCATCAACTTCAAATGTTGTAATTGCACCAGCTGTTGAAGCACAAATCACTGATGA
CTTTTCAGTTGTCCCAGAGAAAAATAAAAATAAAAAGAAGATCAATTCAACTGCAACTGA
TGGTACCACTACCACCACCAATATACCAAAACCAACACCAGTTAGAGCACCAATTACCAA
AAAAGGTAACACTGCTCCACGTTCTCAATTCCATTTATTAGCACTCGATGAAG-------
---CCACTACTAAATCAACTCCAACTCCAACACCAACTCCAACTCCAAGCTGCTACTAAA
GTTGTTGAACAACCAAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAAAGAAATT
AAACAAAATAAAAAAGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTTAATTACT
CCAGGTGTTCCATCCGTAGTTTACAAAATCATTTACGTCGCCCTCATTGGTGTTTTATTA
TTTTCATTAGTACCATTATATTACTCTGGTTTAGATAGTATCTACAGTTATGGTGTCATC
GCTTTAGTTTTAGGTCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCCAAGATTA
CAAGCTTCAAAGGAACAAAAATCAAAATCTGGTAACAAAAAATCAACTACTCGTAAAGTT
AAAGCATAAGTCAATCCCATCATCATCCATTTCCAAANATAAANGTAGATAGGCCTTCAA
TCCCATC
Length of connected seq. 1077
Full length Seq ID -
Full length Seq. -
Length of full length seq. -