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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSH277
Library SS
(Link to library)
Clone ID SSH277
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14946-1
Original site URL
Representative seq. ID SSH277P
(Link to Original site)
Representative DNA sequence 
>SSH277 (SSH277Q) /CSM/SS/SSH2-D/SSH277Q.Seq.d/
AAAAAAAAAAAAAATATTTTTAAAAAAAAAAAATTTTTGAATATTTTTTTTTTTTTTTAT
CAAGCCATTTTTGTTTTCTAATTTTTTTTTTTCATTTGTAATTTTTTGTATAATATCTTG
TATTATTTTTTATAACACTCAAACTATTTATTAAATAAAATGTCCGCCTCAGCAGATCAA
ATTCAAGAATGTTTCAGTATCTTTGATAAAGATAATGACGGTAAGGTCTCAGTTGAAGAT
ATTGGAGCTTGTTTAAGATCATTAGGTAAAAGCCCAACCATGGCAGATATTGAAGCATTA
AAGACTGAAATTGGTGCTAAAGAATTTGATATCAATXXXXXXXXXXAACTTTAAAACTCC
CCAGGGGCAACAAAAGGAAATGTTGGATGCATTCAAAGCCCTCGNTAAAGAAGGCCTTGG
TACCATTCAAGGTGCTGAATTACGTCAATTATTAACCACTCTTGGTGATTACTTATCAAC
CGCTGAAGTTGATGAATTATTTAAGGAAATCTCTGTCGATAGTACTACTGGTGCCGTTTC
ATATGCAAGCTTAGTCAATNCCATCGTTTCTGGTTATCCATTAGGCGGTTTCTAAATTAT
TATTATTTAAAAAAAAAAAAAAAAAAANTGAAATTTATTTTTAAATTATCATAAAATGGA
AAAAAAAAAAAAAAACATTTAAAAAAACACTAAAAAATCCATATAATAAAAAATTGTAAT
AATTAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
sequence update 1998. 9.10
Translated Amino Acid sequence 
kkkknifkkkkflniffffyqaifvf*fffficnflynilyyfl*HSNYLLNKMSASADQ
IQECFSIFDKDNDGKVSVEDIGACLRSLGKSPTMADIEALKTEIGAKEFDIN---

---NFKTPQGQQKEMLDAFKALXKEGLGTIQGAELRQLLTTLGDYLSTAEVDELFKEISV
DSTTGAVSYASLVNXIVSGYPLGGF*iiii*kkkkkx*nlflnyhkmekkkkni*kntkk
si**kiviikkpkkkkkkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
kkkknifkkkkflniffffyqaifvf*fffficnflynilyyfl*HSNYLLNKMSASADQ
IQECFSIFDKDNDGKVSVEDIGACLRSLGKSPTMADIEALKTEIGAKEFDIN---

---NFKTPQGQQKEMLDAFKALXKEGLGTIQGAELRQLLTTLGDYLSTAEVDELFKEISV
DSTTGAVSYASLVNXIVSGYPLGGF*iiii*kkkkkx*nlflnyhkmekkkkni*kntkk
si**kiviikkpkkkkkkkkk

Frame B:
kkkkiflkkknf*ifffffikpflfsnfffsfvifciisciifyntqtiy*ikcppqqik
fknvsvslikimtvrsqlkilelv*dh*vkaqpwqilkh*rlklvlknlis---

---tlklprgnkrkcwmhskpsxkkalvpfkvlnyvny*pllvityqplklmnylrksls
ivllvpfhmqa*sxpsflvih*avsklllfkkkkkkxeiyf*iiikwkkkkktfkktlkn
pynkkl**lknqkkkkkkkkk

Frame C:
kkkkyf*kkkifeyfffflsshfcflifffhl*ffv*ylvlffitlklfik*nvrlsrsn
srmfqyl**r**r*gls*rywslfkiir*kpnhgry*sikd*nwc*ri*yq---

---l*nspgatkgnvgciqspr*rrpwyhsrc*itsiinhsw*llinr*s**ii*gnlcr
*yywcrficklsqxhrfwlsirrflnyyylkkkkkkxkfifkls*ngkkkkkhlkkh*ki
hiikncnn*ktkkkkkkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSH277 (SSH277Q) /CSM/SS/SSH2-D/SSH277Q.Seq.d/                        922   0.0
VSK452 (VSK452Q) /CSM/VS/VSK4-C/VSK452Q.Seq.d/                        456   e-127
VSD769 (VSD769Q) /CSM/VS/VSD7-C/VSD769Q.Seq.d/                        456   e-127
VSD135 (VSD135Q) /CSM/VS/VSD1-B/VSD135Q.Seq.d/                        456   e-127
VSB729 (VSB729Q) /CSM/VS/VSB7-B/VSB729Q.Seq.d/                        456   e-127
SSM811 (SSM811Q) /CSM/SS/SSM8-A/SSM811Q.Seq.d/                        456   e-127
SSM265 (SSM265Q) /CSM/SS/SSM2-C/SSM265Q.Seq.d/                        456   e-127
SSL189 (SSL189Q) /CSM/SS/SSL1-D/SSL189Q.Seq.d/                        456   e-127
SSK774 (SSK774Q) /CSM/SS/SSK7-D/SSK774Q.Seq.d/                        456   e-127
SSK366 (SSK366Q) /CSM/SS/SSK3-C/SSK366Q.Seq.d/                        456   e-127
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X54161|X54161.1 D.discoideum myosin essential light chain gene.   456   0.0    5
M19337|M19337.1 D.discoideum myosin light chain mRNA, complete cds.   454   0.0    2
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    38   3e-06  15
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    32   3e-05  14
AE014840|AE014840.1 Plasmodium falciparum 3D7 chromosome 11 section 5 of 8 of the complete sequence.    36   4e-05  12
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    38   5e-05  15
AE014818|AE014818.1 Plasmodium falciparum 3D7 chromosome 14 section 3 of 13 of the complete sequence.    36   7e-05  15
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    32   8e-05  12
AY392440|AY392440.1 Dictyostelium discoideum probable 3-oxoacyl-acyl-carrier protein synthase gene, complete cds.    48   1e-04  3
AE014829|AE014829.1 Plasmodium falciparum 3D7 chromosome 10 section 1 of 7 of the complete sequence.    32   2e-04  13
dna update 2004. 2.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P09402) RecName: Full=Myosin, essential light chain; AltName: F...   149   5e-64
A28127(A28127;B48404;A48404;S20728) myosin light chain, nonmuscl...   140   2e-61
A29910(A29910)myosin calcium-binding light chain - slime mold (P...    95   2e-38
(P08053) RecName: Full=Myosin regulatory light chain; AltName: F...    95   5e-38
J03499_1(J03499|pid:none) Physarum polycephalum myosin calcium-b...    95   6e-38
DQ122882_1(DQ122882|pid:none) Chlamydomonas incerta calmodulin m...    57   8e-16
(P04352) RecName: Full=Calmodulin;          Short=CaM;  &M20729_...    57   8e-16
(P27165) RecName: Full=Calmodulin;          Short=CaM;  &(Q71UH5...    57   8e-16
(P41041) RecName: Full=Calmodulin;          Short=CaM;  &FB33590...    55   8e-16
AF084426_1(AF084426|pid:none) Synthetic construct calmodulin mut...    56   1e-15
protein update 2009. 6.12
PSORT 

 psg: 0.73  gvh: 0.47  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SSH277 is cyt
5' end seq. ID SSH277F
5' end seq. 
>SSH277F.Seq
AAAAAAAAAAAAAATATTTTTAAAAAAAAAAAATTTTTGAATATTTTTTTTTTTTTTTAT
CAAGCCATTTTTGTTTTCTAATTTTTTTTTTTCATTTGTAATTTTTTGTATAATATCTTG
TATTATTTTTTATAACACTCAAACTATTTATTAAATAAAATGTCCGCCTCAGCAGATCAA
ATTCAAGAATGTTTCAGTATCTTTGATAAAGATAATGACGGTAAGGTCTCAGTTGAAGAT
ATTGGAGCTTGTTTAAGATCATTAGGTAAAAGCCCAACCATGGCAGATATTGAAGCATTA
AAGACTGAAATTGGTGCTAAAGAATTTGATATCAAT----------
Length of 5' end seq. 336
3' end seq. ID SSH277Z
3' end seq. 
>SSH277Z.Seq
----------AACTTTAAAACTCCCCAGGGGCAACAAAAGGAAATGTTGGATGCATTCAA
AGCCCTCGNTAAAGAAGGCCTTGGTACCATTCAAGGTGCTGAATTACGTCAATTATTAAC
CACTCTTGGTGATTACTTATCAACCGCTGAAGTTGATGAATTATTTAAGGAAATCTCTGT
CGATAGTACTACTGGTGCCGTTTCATATGCAAGCTTAGTCAATNCCATCGTTTCTGGTTA
TCCATTAGGCGGTTTCTAAATTATTATTATTTAAAAAAAAAAAAAAAAAAANTGAAATTT
ATTTTTAAATTATCATAAAATGGAAAAAAAAAAAAAAAACATTTAAAAAAACACTAAAAA
ATCCATATAATAAAAAATTGTAATAATTAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAA
AAAAA
Length of 3' end seq. 415
Connected seq. ID SSH277P
Connected seq. 
>SSH277P.Seq
AAAAAAAAAAAAAATATTTTTAAAAAAAAAAAATTTTTGAATATTTTTTTTTTTTTTTAT
CAAGCCATTTTTGTTTTCTAATTTTTTTTTTTCATTTGTAATTTTTTGTATAATATCTTG
TATTATTTTTTATAACACTCAAACTATTTATTAAATAAAATGTCCGCCTCAGCAGATCAA
ATTCAAGAATGTTTCAGTATCTTTGATAAAGATAATGACGGTAAGGTCTCAGTTGAAGAT
ATTGGAGCTTGTTTAAGATCATTAGGTAAAAGCCCAACCATGGCAGATATTGAAGCATTA
AAGACTGAAATTGGTGCTAAAGAATTTGATATCAAT----------AACTTTAAAACTCC
CCAGGGGCAACAAAAGGAAATGTTGGATGCATTCAAAGCCCTCGNTAAAGAAGGCCTTGG
TACCATTCAAGGTGCTGAATTACGTCAATTATTAACCACTCTTGGTGATTACTTATCAAC
CGCTGAAGTTGATGAATTATTTAAGGAAATCTCTGTCGATAGTACTACTGGTGCCGTTTC
ATATGCAAGCTTAGTCAATNCCATCGTTTCTGGTTATCCATTAGGCGGTTTCTAAATTAT
TATTATTTAAAAAAAAAAAAAAAAAAANTGAAATTTATTTTTAAATTATCATAAAATGGA
AAAAAAAAAAAAAAACATTTAAAAAAACACTAAAAAATCCATATAATAAAAAATTGTAAT
AATTAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Length of connected seq. 751
Full length Seq ID -
Full length Seq. -
Length of full length seq. -