>AFL395 (AFL395Q) /CSM/AF/AFL3-D/AFL395Q.Seq.d/
AACTTTTGTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTNTTACTCGGTTCTG
GCTTTGTTGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATATTTTGTAACAAT
TGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATT
CAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAA
ATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATG
TATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATT
ATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGG
TATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGT
CACATCATTCGTTTCATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATT
TGGATACAAATTTTCATGGTCACXXXXXXXXXXGTGGTTTTGGAATCATGATCCGTGCAC
TTGTTGCCGTTGGTTTATTCTCCACTGAAAGTTGATGCACGTCTCGCTGTTGAGGGTGGT
ATTTCATGGCGTAACTATCTCGTTCAGCTATTGGGTTGCAATGATAATGATAGTGACCTC
CTATGTTGTGTGGAATCCACCATTAAAGAGTACTTTGAAAAATTAAAGACTGAGAGAGAC
GGCTTACAATTCCATTTCCCAATTATTCCAAGAGATATTGAAAAAGATGTTCAACATGCC
GTTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGCTGATGAAAAAGTTGTAAATAAGAAT
ACTCCAATCGATAGTCTTTGCGCTTTACTCGAAAAGAAACTCTCTTACAAAGCTGGTGAA
CGTGATGTAGTTGTCCTTGAACATAACTTTGTCGTCCAATACGCTGATCGTACAGAAAAA
GAAGTTTCATCACTCATTTGTTATGGTATTCCAAATGGTAGTTCTGCAACTTCACTCACT
GTTGGTGTTCCAGTTGGTATCGCAACTGAACTCATCGCTGATGGTAAAACTACAACTAGA
GGTGTTGTTGGTCCAGTAACTCCTNAATTTATTTACCAATTTTAGAGAAATTAAAATCNA
AAA

tfvyaeekkrnekyixtrfwlcckast*LPLKKRRYFVTIVSLFQNELDSITKGHDTSKF
KTIQLDVMNKLNELEEYFPKSDCVISLIPATLHSTVAKLCIKHKTHLVTASYISDDMKAL
SEEAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWCGALPSTECADNPF
GYKFSWS---

---GFGIMIRALVAVGLFSTES*ctsrc*gwyfma*lsrsaiglq*****ppmlcgihh*
rvl*kikd*errltipfpnyskry*krcstcr*rfqmvgfikc**ksck*eysnr*slrf
trketllqsw*t*cscp*t*lcrpir*syrkrsfithllwyskw*fcnfthcwcsswyrn
*thr*w*nyn*rccwssnsxiylpileklksk

Frame A:
nfcirrrkkkk*kiyxysvlallqsqhlits*kekifcnnc*fipk*irlnykrsryiki
qnnsircye*ie*irrifpki*lcn*fnssnitfnsckimy*t*ntfsysklyfr*yesi
irrskggrcfiie*tws*sry*syvinedy*sc*gkww*ghiirfmvwcatinrmc*qsi
wiqifmv---

---vvles*svhllplvysplkvdarlaveggiswrnylvqllgcndndsdllccvesti
keyfeklkterdglqfhfpiiprdiekdvqhavegfkwlgllsadekvvnkntpidslca
llekklsykagerdvvvlehnfvvqyadrtekevsslicygipngssatsltvgvpvgia
teliadgktttrgvvgpvtpxfiyqf*rn*nxk

Frame B:
tfvyaeekkrnekyixtrfwlcckast*LPLKKRRYFVTIVSLFQNELDSITKGHDTSKF
KTIQLDVMNKLNELEEYFPKSDCVISLIPATLHSTVAKLCIKHKTHLVTASYISDDMKAL
SEEAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENGGKVTSFVSWCGALPSTECADNPF
GYKFSWS---

---wfwnhdpctccrwfilh*klmhvsllrvvfhgvtisfsywvamimivtsyvvwnppl
kstlkn*rlretaynsisqlfqeilkkmfnmplkvsngwvy*vlmkkl*irilqsivfal
yskrnsltklvnvm*lslnitlssntlivqkkkfhhsfvmvfqmvvlqlhsllvfqlvsq
lnsslmvklqlevllvq*llnlftnfreikix

Frame C:
llytqkkkkemknixllgsgfvakpaldyllkredil*qllvyskmn*tqlqkvtihqns
kqfn*ml*in*mn*knisqnliv*lv*fqqhyiqqlqnyvlnikhi*lqqvifqmi*khy
qkkqrrqvfyy*mnlvliqvliichq*rllimlrkmvvrshhsfhgvvryhqqnvltihl
dtnfhgh---

---GFGIMIRALVAVGLFSTES*ctsrc*gwyfma*lsrsaiglq*****ppmlcgihh*
rvl*kikd*errltipfpnyskry*krcstcr*rfqmvgfikc**ksck*eysnr*slrf
trketllqsw*t*cscp*t*lcrpir*syrkrsfithllwyskw*fcnfthcwcsswyrn
*thr*w*nyn*rccwssnsxiylpileklksk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL395 (AFL395Q) /CSM/AF/AFL3-D/AFL395Q.Seq.d/                       2254   0.0
VFJ670 (VFJ670Q) /CSM/VF/VFJ6-C/VFJ670Q.Seq.d/                       1213   0.0
AFK445 (AFK445Q) /CSM/AF/AFK4-B/AFK445Q.Seq.d/                       1213   0.0
VFC570 (VFC570Q) /CSM/VF/VFC5-C/VFC570Q.Seq.d/                       1207   0.0
VFL645 (VFL645Q) /CSM/VF/VFL6-B/VFL645Q.Seq.d/                       1199   0.0
VFJ362 (VFJ362Q) /CSM/VF/VFJ3-C/VFJ362Q.Seq.d/                       1199   0.0
CFI658 (CFI658Q) /CSM/CF/CFI6-C/CFI658Q.Seq.d/                       1199   0.0
AFO224 (AFO224Q) /CSM/AF/AFO2-A/AFO224Q.Seq.d/                       1199   0.0
AFO144 (AFO144Q) /CSM/AF/AFO1-B/AFO144Q.Seq.d/                       1199   0.0
AFO141 (AFO141Q) /CSM/AF/AFO1-B/AFO141Q.Seq.d/                       1199   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL394098|AL394098.1 T3 end of clone AR0AA015A07 of library AR0AA from strain CBS 732 ofZygosaccharomyces rouxii.    46   0.002  2
AZ928392|AZ928392.1 479.dif09b09.s1 Saccharomyces kluyveri Saccharomyces kluyveri genomic clone 479.dif09b09.s1, DNA sequence.    40   0.004  2
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          32   0.008  24
AE017339|AE017339.1 Mycoplasma mobile 163K section 3 of 3 of the complete genome.    34   0.014  15
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    38   0.016  14
AC138524|AC138524.1 Homo sapiens chromosome 5 clone XXyac-4X104D9, WORKING DRAFT SEQUENCE, 308 unordered pieces.    52   0.021  1
AX829926|AX829926.1 Sequence 646 from Patent WO03072602.           52   0.021  1
AX818896|AX818896.1 Sequence 646 from Patent EP1338608.            52   0.021  1
AX594978|AX594978.1 Sequence 632 from Patent EP1258494.            52   0.021  1
X77363|X77363.1 S.cerevisiae LYS9 gene.                            52   0.021  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NG9) RecName: Full=Probable saccharopine dehydrogenase [NADP...   332   8e-95
FM992690_618(FM992690|pid:none) Candida dubliniensis CD36 chromo...   140   6e-36
BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...   146   1e-35
CR382130_918(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   139   2e-34
AM989986_8(AM989986|pid:none) Zygosaccharomyces rouxii strain CB...   134   6e-34
AY751465_1(AY751465|pid:none) Oncorhynchus mykiss lysine ketoglu...   135   2e-33
CR382138_765(CR382138|pid:none) Debaryomyces hansenii strain CBS...   133   2e-33
(Q9SMZ4) RecName: Full=Alpha-aminoadipic semialdehyde synthase; ...   140   5e-33
U95758_1(U95758|pid:none) Arabidopsis thaliana lysine-ketoglutar...   140   5e-33
(P38999) RecName: Full=Saccharopine dehydrogenase [NADP+, L-glut...   130   8e-33

 psg: 0.96  gvh: 0.46  alm: 0.34  top: 0.13  tms: 0.00  mit: 0.36  mip: 0.03
 nuc: 0.10  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFL395 is cyt

>AFL395F.Seq
AACTTTTGTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTNTTACTCGGTTCTG
GCTTTGTTGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATATTTTGTAACAAT
TGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATT
CAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAA
ATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATG
TATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATT
ATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGG
TATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGT
CACATCATTCGTTTCATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATT
TGGATACAAATTTTCATGGTCAC----------

>AFL395Z.Seq
----------GTGGTTTTGGAATCATGATCCGTGCACTTGTTGCCGTTGGTTTATTCTCC
ACTGAAAGTTGATGCACGTCTCGCTGTTGAGGGTGGTATTTCATGGCGTAACTATCTCGT
TCAGCTATTGGGTTGCAATGATAATGATAGTGACCTCCTATGTTGTGTGGAATCCACCAT
TAAAGAGTACTTTGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAAT
TATTCCAAGAGATATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGG
TTTATTAAGTGCTGATGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGC
TTTACTCGAAAAGAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACA
TAACTTTGTCGTCCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTA
TGGTATTCCAAATGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGC
AACTGAACTCATCGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTCC
TNAATTTATTTACCAATTTTAGAGAAATTAAAATCNAAAA

>AFL395P.Seq
AACTTTTGTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTNTTACTCGGTTCTG
GCTTTGTTGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATATTTTGTAACAAT
TGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATACATCAAAATT
CAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAATATTTCCCAAA
ATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTGCAAAATTATG
TATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATATGAAAGCATT
ATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTCTTGATCCAGG
TATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATGGTGGTAAGGT
CACATCATTCGTTTCATGGTGTGGTGCGCTACCATCAACAGAATGTGCTGACAATCCATT
TGGATACAAATTTTCATGGTCAC----------GTGGTTTTGGAATCATGATCCGTGCAC
TTGTTGCCGTTGGTTTATTCTCCACTGAAAGTTGATGCACGTCTCGCTGTTGAGGGTGGT
ATTTCATGGCGTAACTATCTCGTTCAGCTATTGGGTTGCAATGATAATGATAGTGACCTC
CTATGTTGTGTGGAATCCACCATTAAAGAGTACTTTGAAAAATTAAAGACTGAGAGAGAC
GGCTTACAATTCCATTTCCCAATTATTCCAAGAGATATTGAAAAAGATGTTCAACATGCC
GTTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGCTGATGAAAAAGTTGTAAATAAGAAT
ACTCCAATCGATAGTCTTTGCGCTTTACTCGAAAAGAAACTCTCTTACAAAGCTGGTGAA
CGTGATGTAGTTGTCCTTGAACATAACTTTGTCGTCCAATACGCTGATCGTACAGAAAAA
GAAGTTTCATCACTCATTTGTTATGGTATTCCAAATGGTAGTTCTGCAACTTCACTCACT
GTTGGTGTTCCAGTTGGTATCGCAACTGAACTCATCGCTGATGGTAAAACTACAACTAGA
GGTGTTGTTGGTCCAGTAACTCCTNAATTTATTTACCAATTTTAGAGAAATTAAAATCNA
AAA