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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB357
Library AF
(Link to library)
Clone ID AFB357
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U14942-1
Original site URL
Representative seq. ID AFB357E
(Link to Original site)
Representative DNA sequence 
>AFB357 (AFB357Q) /CSM/AF/AFB3-C/AFB357Q.Seq.d/
GTTAAAAAAGCATATTTTAAAGGTATTTCTGTTGAAGATTTAAAATTTTTATTTTTACAA
AAGTTTCCAGAATTTCCAAAAGATGTTGATATTCAATTCCTTATAATTGATAAAGGTACA
AATAAAGAACAACAAGTTATTGATGTTAATGATATAAAAGAACATCAAGAATTATTAATT
AGACATGAGTATAATGAAGATACAAAAAAAAGATCACAAGGTTATACAGGATTAGATAAA
GAAAAGATAGTTTTAGTAATGGTTGGATTACCAGCAAGAGGTAAAACATATGTTGCAAGA
AAGATATGTAGAATGTTAAATTGGATGTTAGTACCAGCCAAGGTGTTTAATGTTGGAGAA
TATAGAAGATTAAGAATTGGTGCAGGACAACCACATGACTTCTTTGATCCACATAATCCA
GAGGGTATAAAGGCGAGATTGCATATGGCAGTTGCGGCATTGGACGACATGATATCATGG
TTGCATAACGGTGGTAGGGTTGGTATTTACGATGCGACCAACTCCACCAACGAGAGAAGA
CAATTAGTAGAGAAAAGATGCACTCGTGAAAAGATGACTGTGGTTTATGTAGAATCAATT
TGTAATGATGCATCAGTGATCGAACATAACATTAAGGAGACCAAACTATTGTCACCCGAT
TACCAGGGTGTGGATCCCTCCAAGGCCGTGCAAGACTTTCGTGATAGAATCAATCATTAT
CTAAAAGTGTATGAGACTGTGGAGGGCGAGAAAACTCGCTACATAAAGCTATTCGATGTT
GGGAAGAAGATCGAGGTGAATAACATCACCGGGTACATACCAAGCAGGGTGGTGTTCTTC
CTCATGAACCTCCACCTCCACCCCCGTCCCATCTGGTTGACCCGTCATGGCGAGAGCGAG
TTCAACGCCCAAAATCGTATTGGTGGCGACAGCGACCTCACTGAACGTGGCGATAACTAC
GCCCACCAATTGGCCGTTTGGATTAGTGAATGGTGTTCAAAAGTNCGTGCTGACGGCTAC
GATGGTGAAGTTGTGGTATGGACCTCAAGTTTGAAACGTGCAATTCGTATAGCTCAATAT
ATCTCTCAACCAAAGGTTGTAATGAGAGCATTGGATGAAATTGATGCTGGTGTTTGCGAT
GGTATGACCTATGAAGAAATTCAAGAGAAAATGCCAGATGAAAGGGCTGCTCGTGATTCT
GATAAACTTCAATATCGTTATCCAAGAGGTGAAAGTTATGAAGATGTAATTCAACGTCTT
GAACCTCTATTATTAGAATTGGAAAGAACTAAATCACCAGTCCTAATCGTTTCTCATCAA
GCCACTCTTCGTTGTCTCTATTCTTATCTCAAAGGTAGAGTTAAAGAAGAATGCCCCTTC
ATTAATATTCCATTACATACTTTAATTCAATTATCACCAAAAGCATAG
sequence update 2003. 5. 3
Translated Amino Acid sequence 
VKKAYFKGISVEDLKFLFLQKFPEFPKDVDIQFLIIDKGTNKEQQVIDVNDIKEHQELLI
RHEYNEDTKKRSQGYTGLDKEKIVLVMVGLPARGKTYVARKICRMLNWMLVPAKVFNVGE
YRRLRIGAGQPHDFFDPHNPEGIKARLHMAVAALDDMISWLHNGGRVGIYDATNSTNERR
QLVEKRCTREKMTVVYVESICNDASVIEHNIKETKLLSPDYQGVDPSKAVQDFRDRINHY
LKVYETVEGEKTRYIKLFDVGKKIEVNNITGYIPSRVVFFLMNLHLHPRPIWLTRHGESE
FNAQNRIGGDSDLTERGDNYAHQLAVWISEWCSKVRADGYDGEVVVWTSSLKRAIRIAQY
ISQPKVVMRALDEIDAGVCDGMTYEEIQEKMPDERAARDSDKLQYRYPRGESYEDVIQRL
EPLLLELERTKSPVLIVSHQATLRCLYSYLKGRVKEECPFINIPLHTLIQLSPKA*
Translated Amino Acid sequence (All Frames) 
Frame A:
VKKAYFKGISVEDLKFLFLQKFPEFPKDVDIQFLIIDKGTNKEQQVIDVNDIKEHQELLI
RHEYNEDTKKRSQGYTGLDKEKIVLVMVGLPARGKTYVARKICRMLNWMLVPAKVFNVGE
YRRLRIGAGQPHDFFDPHNPEGIKARLHMAVAALDDMISWLHNGGRVGIYDATNSTNERR
QLVEKRCTREKMTVVYVESICNDASVIEHNIKETKLLSPDYQGVDPSKAVQDFRDRINHY
LKVYETVEGEKTRYIKLFDVGKKIEVNNITGYIPSRVVFFLMNLHLHPRPIWLTRHGESE
FNAQNRIGGDSDLTERGDNYAHQLAVWISEWCSKVRADGYDGEVVVWTSSLKRAIRIAQY
ISQPKVVMRALDEIDAGVCDGMTYEEIQEKMPDERAARDSDKLQYRYPRGESYEDVIQRL
EPLLLELERTKSPVLIVSHQATLRCLYSYLKGRVKEECPFINIPLHTLIQLSPKA*


Frame B:
lkkhilkvfllki*nfyfyksfqnfqkmlifnsl*likvqiknnkllmlmi*knikny*l
dmsimkiqkkdhkviqd*ikkr*f**wldyqqevkhmlqeryvec*igc*yqprclmlen
ied*elvqdnhmtslihiiqrv*rrdciwqlrhwtt*yhgcitvvglvftmrptpptred
n**rkdalvkr*lwfm*nqfvmmhq*snitlrrpnychpitrvwipprpcktfviesiii
*kcmrlwrarklat*sysmlgrrsr*itspgtyqagwcsss*tststpvpsg*pvmaras
stpkivlvatatslnvaittptnwpfglvngvqkxvltatmvklwygpqv*nvqfv*lni
slnqrl**ehwmklmlvfamv*pmkkfkrkcqmkgllvilinfniviqevkvmkm*fnvl
nlyy*nwkelnhqs*sflikplfvvsiliskvelkknapslifhyil*fnyhqkh


Frame C:
*ksif*ryfc*rfkififtkvsriskrc*ysipyn**ryk*rttsy*c**ykrtsriin*
t*v**rykkkitrlyrir*rkdsfsngwitskr*nicckkdm*nvkldvstsqgv*cwri
*kiknwcrttt*ll*st*srgykgeiaygscgigrhdimva*rw*gwylrcdqlhqrekt
isrekmhs*kddcglcrinl**cisdrt*h*gdqtivtrlpgcgslqgrarls**nqsls
ksv*dcggrenslhkaircweedrge*hhrvhtkqggvlpheppppppshlvdpswrerv
qrpksywwrqrph*twr*lrppigrld**mvfksxc*rlrw*scgmdlkfetcnsyssiy
lstkgcnesig*n*cwclrwydl*rnsrenar*kgcs*f**tsislskr*kl*rcnsts*
tsiirigkn*itspnrfssshsslslflsqr*s*rrmplh*ysityfnsiitksi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB357 (AFB357Q) /CSM/AF/AFB3-C/AFB357Q.Seq.d/                       2777   0.0
AFO207 (AFO207Q) /CSM/AF/AFO2-A/AFO207Q.Seq.d/                       1261   0.0
SFC253 (SFC253Q) /CSM/SF/SFC2-C/SFC253Q.Seq.d/                        993   0.0
SSL191 (SSL191Q) /CSM/SS/SSL1-D/SSL191Q.Seq.d/                         96   2e-18
VFD255 (VFD255Q) /CSM/VF/VFD2-C/VFD255Q.Seq.d/                         62   2e-08
SHA888 (SHA888Q) /CSM/SH/SHA8-D/SHA888Q.Seq.d/                         44   0.006
VSB624 (VSB624Q) /CSM/VS/VSB6-A/VSB624Q.Seq.d/                         38   0.36
VHL579 (VHL579Q) /CSM/VH/VHL5-D/VHL579Q.Seq.d/                         38   0.36
VHG679 (VHG679Q) /CSM/VH/VHG6-D/VHG679Q.Seq.d/                         38   0.36
VHF425 (VHF425Q) /CSM/VH/VHF4-B/VHF425Q.Seq.d/                         38   0.36
own update 2004. 8. 6
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    70  4e-14  6
BZ297835|BZ297835.1 CG3662.f1 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG3662, DNA sequence.    66  1e-06  1
AZ925805|AZ925805.1 476.dio03d03.s1 Saccharomyces castellii NRRL Y-12630 Saccharomyces castellii genomic clone 476.dio03d03.s1, DNA sequence.    56  5e-05  2
AU210143|AU210143.1 Caenorhabditis elegans cDNA clone:yk753f12 : 3' end, single read.    54  0.005  1
AL402109|AL402109.1 T3 end of clone XAS0AA001H07 of library XAS0AA from strain CLIB 533  of Saccharomyces bayanus.    54  0.005  1
BJ142665|BJ142665.1 Caenorhabditis elegans cDNA clone:yk1187b05 : 3' end, single read.    54  0.005  1
AU217025|AU217025.1 Caenorhabditis elegans cDNA clone:yk844h10 : 3' end, single read.    54  0.005  1
U41558|U41558.1 Caenorhabditis elegans cosmid K02B2, complete sequence.    54  0.026  2
BH653930|BH653930.1 BOMCQ28TR BO_2_3_KB Brassica oleracea genomic clone BOMCQ28, DNA sequence.    38  0.044  2
AC006058|AC006058.1 Homo sapiens 3p22-7 PAC RPCI4-555D20 (Roswell Park Cancer Institute Human PAC Library) complete sequence.    46  0.12  2
dna update 2003. 9. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM746676_36(AM746676|pid:none) Sorangium cellulosum 'So ce 56' c...   455   e-126
FB879171_1(FB879171|pid:none) Sequence 98444 from Patent WO20080...   376   e-103
AP008209_1173(AP008209|pid:none) Oryza sativa (japonica cultivar...   373   e-101
AF242859_1(AF242859|pid:none) Arabidopsis thaliana fructose-6-ph...   373   e-101
AB035288_1(AB035288|pid:none) Arabidopsis thaliana fkfbp mRNA fo...   373   e-101
AC067971_19(AC067971|pid:none) Sequence of BAC F10K1 from Arabid...   373   e-101
BT033471_1(BT033471|pid:none) Zea mays full-length cDNA clone ZM...   371   e-101
FB879153_1(FB879153|pid:none) Sequence 98426 from Patent WO20080...   371   e-101
AP008211_368(AP008211|pid:none) Oryza sativa (japonica cultivar-...   371   e-101
AC124144_14(AC124144|pid:none) Oryza sativa (japonica cultivar-g...   371   e-101
protein update 2009. 3.27
PSORT 

 psg: 0.59  gvh: 0.24  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for AFB357 is cyt
5' end seq. ID AFB357F
5' end seq. 
>AFB357F.Seq
GTTAAAAAAGCATATTTTAAAGGTATTTCTGTTGAAGATTTAAAATTTTTATTTTTACAA
AAGTTTCCAGAATTTCCAAAAGATGTTGATATTCAATTCCTTATAATTGATAAAGGTACA
AATAAAGAACAACAAGTTATTGATGTTAATGATATAAAAGAACATCAAGAATTATTAATT
AGACATGAGTATAATGAAGATACAAAAAAAAGATCACAAGGTTATACAGGATTAGATAAA
GAAAAGATAGTTTTAGTAATGGTTGGATTACCAGCAAGAGGTAAAACATATGTTGCAAGA
AAGATATGTAGAATGTTAAATTGGATGTTAGTACCAGCCAAGGTGTTTAATGTTGGAGAA
TATAGAAGATTAAGAATTGGTGCAGGACAACCACATGACTTCTTTGATCCACATAATCCA
GAGGGTATAAAGGCGAGATTGCATATGGCAGTTGCGGCATTGGACGACATGATATCATGG
TTGCATAACGGTGGTAGGGTTGGTATTTACGATGCGACCAACTCCACCAACGAGAGAAGA
CAATTAGTAGAGAAAAGATGCACTCGTGAAAAGATGACTGTGGTTTATGTAGAATCAATT
TGTAATGATGCATCAGTGATCGAACATAACATTAAGGAGACCAAACTATTGTCACCCGAT
TACCAGGGTGTGGATCCCTCCAAGGCCGTGCAAGACTTTCGTGATAGAATCAATCATTAT
CTAAAAGTGTATGAGACTGTGGAGGGCGAGAAAACTCGCTACATAAAGCTATTCGATGTT
GGGAAGAAGATCGAGGTGAATAACATCACCGGGTACATACCAAGCAGGGTGGTGTTCTTC
CTCATGAACCTCCACCTCCACCCCCGTCCCATCTGGTTGACCCGTCATGGCGAGAGCGAG
TTCAACGCCCAAAATCGTATTGGTGGCGACAGCGACCTCACTGAACGTGGCGATAACTAC
GCCCACCAATTGGCCGTTTGGATTAGTGAATGGTGTTCAAAAGTGCGTGCTGACGGCTAC
GATGGTGANNNNNNNNNN
Length of 5' end seq. 1038
3' end seq. ID AFB357Z
3' end seq. 
>AFB357Z.Seq
----------TTCGATGTTGGGAAGAAGATCGAGGTGAATAACATCACCGGGTACATACC
AAGCAGGGTGGTGTTCTTCCTCATGAACCTCCACCTCCACCCCCGTCCCATCTGGTTGAC
CCGTCATGGCGAGAGCGAGTTCAACGCCCAAAATCGTATTGGTGGCGACAGCGACCTCAC
TGAACGTGGCGATAACTACGCCCACCAATTGGCCGTTTGGATTAGTGAATGGTGTTCAAA
AGTCCGTGCTGACGGCTACGATGGTGAAGTTGTGGTATGGACCTCAAGTTTGAAACGTGC
AATTCGTATAGCTCAATATATCTCTCAACCAAAGGTTGTAATGAGAGCATTGGATGAAAT
TGATGCTGGTGTTTGCGATGGTATGACCTATGAAGAAATTCAAGAGAAAATGCCAGATGA
AAGGGCTGCTCGTGATTCTGATAAACTTCAATATCGTTATCCAAGAGGTGAAAGTTATGA
AGATGTAATTCAACGTCTTGAACCTCTATTATTAGAATTGGAAAGAACTAAATCACCAGT
CCTAATCGTTTCTCATCAAGCCACTCTTCGTTGTCTCTATTCTTATCTCAAAGGTAGAGT
TAAAGAAGAATGCCCCTTCATTAATATTCCATTACATACTTTAATTCAATTATCACCAAA
AGCATAG
Length of 3' end seq. 657
Connected seq. ID AFB357P
Connected seq. 
>AFB357P.Seq
GTTAAAAAAGCATATTTTAAAGGTATTTCTGTTGAAGATTTAAAATTTTTATTTTTACAA
AAGTTTCCAGAATTTCCAAAAGATGTTGATATTCAATTCCTTATAATTGATAAAGGTACA
AATAAAGAACAACAAGTTATTGATGTTAATGATATAAAAGAACATCAAGAATTATTAATT
AGACATGAGTATAATGAAGATACAAAAAAAAGATCACAAGGTTATACAGGATTAGATAAA
GAAAAGATAGTTTTAGTAATGGTTGGATTACCAGCAAGAGGTAAAACATATGTTGCAAGA
AAGATATGTAGAATGTTAAATTGGATGTTAGTACCAGCCAAGGTGTTTAATGTTGGAGAA
TATAGAAGATTAAGAATTGGTGCAGGACAACCACATGACTTCTTTGATCCACATAATCCA
GAGGGTATAAAGGCGAGATTGCATATGGCAGTTGCGGCATTGGACGACATGATATCATGG
TTGCATAACGGTGGTAGGGTTGGTATTTACGATGCGACCAACTCCACCAACGAGAGAAGA
CAATTAGTAGAGAAAAGATGCACTCGTGAAAAGATGACTGTGGTTTATGTAGAATCAATT
TGTAATGATGCATCAGTGATCGAACATAACATTAAGGAGACCAAACTATTGTCACCCGAT
TACCAGGGTGTGGATCCCTCCAAGGCCGTGCAAGACTTTCGTGATAGAATCAATCATTAT
CTAAAAGTGTATGAGACTGTGGAGGGCGAGAAAACTCGCTACATAAAGCTATTCGATGTT
GGGAAGAAGATCGAGGTGAATAACATCACCGGGTACATACCAAGCAGGGTGGTGTTCTTC
CTCATGAACCTCCACCTCCACCCCCGTCCCATCTGGTTGACCCGTCATGGCGAGAGCGAG
TTCAACGCCCAAAATCGTATTGGTGGCGACAGCGACCTCACTGAACGTGGCGATAACTAC
GCCCACCAATTGGCCGTTTGGATTAGTGAATGGTGTTCAAAAGTGCGTGCTGACGGCTAC
GATGGTGA----------TTCGATGTTGGGAAGAAGATCGAGGTGAATAACATCACCGGG
TACATACCAAGCAGGGTGGTGTTCTTCCTCATGAACCTCCACCTCCACCCCCGTCCCATC
TGGTTGACCCGTCATGGCGAGAGCGAGTTCAACGCCCAAAATCGTATTGGTGGCGACAGC
GACCTCACTGAACGTGGCGATAACTACGCCCACCAATTGGCCGTTTGGATTAGTGAATGG
TGTTCAAAAGTCCGTGCTGACGGCTACGATGGTGAAGTTGTGGTATGGACCTCAAGTTTG
AAACGTGCAATTCGTATAGCTCAATATATCTCTCAACCAAAGGTTGTAATGAGAGCATTG
GATGAAATTGATGCTGGTGTTTGCGATGGTATGACCTATGAAGAAATTCAAGAGAAAATG
CCAGATGAAAGGGCTGCTCGTGATTCTGATAAACTTCAATATCGTTATCCAAGAGGTGAA
AGTTATGAAGATGTAATTCAACGTCTTGAACCTCTATTATTAGAATTGGAAAGAACTAAA
TCACCAGTCCTAATCGTTTCTCATCAAGCCACTCTTCGTTGTCTCTATTCTTATCTCAAA
GGTAGAGTTAAAGAAGAATGCCCCTTCATTAATATTCCATTACATACTTTAATTCAATTA
TCACCAAAAGCATAG
Length of connected seq. 1685
Full length Seq ID AFB357E
Full length Seq. 
>AFB357E.Seq
GTTAAAAAAGCATATTTTAAAGGTATTTCTGTTGAAGATTTAAAATTTTTATTTTTACAA
AAGTTTCCAGAATTTCCAAAAGATGTTGATATTCAATTCCTTATAATTGATAAAGGTACA
AATAAAGAACAACAAGTTATTGATGTTAATGATATAAAAGAACATCAAGAATTATTAATT
AGACATGAGTATAATGAAGATACAAAAAAAAGATCACAAGGTTATACAGGATTAGATAAA
GAAAAGATAGTTTTAGTAATGGTTGGATTACCAGCAAGAGGTAAAACATATGTTGCAAGA
AAGATATGTAGAATGTTAAATTGGATGTTAGTACCAGCCAAGGTGTTTAATGTTGGAGAA
TATAGAAGATTAAGAATTGGTGCAGGACAACCACATGACTTCTTTGATCCACATAATCCA
GAGGGTATAAAGGCGAGATTGCATATGGCAGTTGCGGCATTGGACGACATGATATCATGG
TTGCATAACGGTGGTAGGGTTGGTATTTACGATGCGACCAACTCCACCAACGAGAGAAGA
CAATTAGTAGAGAAAAGATGCACTCGTGAAAAGATGACTGTGGTTTATGTAGAATCAATT
TGTAATGATGCATCAGTGATCGAACATAACATTAAGGAGACCAAACTATTGTCACCCGAT
TACCAGGGTGTGGATCCCTCCAAGGCCGTGCAAGACTTTCGTGATAGAATCAATCATTAT
CTAAAAGTGTATGAGACTGTGGAGGGCGAGAAAACTCGCTACATAAAGCTATTCGATGTT
GGGAAGAAGATCGAGGTGAATAACATCACCGGGTACATACCAAGCAGGGTGGTGTTCTTC
CTCATGAACCTCCACCTCCACCCCCGTCCCATCTGGTTGACCCGTCATGGCGAGAGCGAG
TTCAACGCCCAAAATCGTATTGGTGGCGACAGCGACCTCACTGAACGTGGCGATAACTAC
GCCCACCAATTGGCCGTTTGGATTAGTGAATGGTGTTCAAAAGTNCGTGCTGACGGCTAC
GATGGTGAAGTTGTGGTATGGACCTCAAGTTTGAAACGTGCAATTCGTATAGCTCAATAT
ATCTCTCAACCAAAGGTTGTAATGAGAGCATTGGATGAAATTGATGCTGGTGTTTGCGAT
GGTATGACCTATGAAGAAATTCAAGAGAAAATGCCAGATGAAAGGGCTGCTCGTGATTCT
GATAAACTTCAATATCGTTATCCAAGAGGTGAAAGTTATGAAGATGTAATTCAACGTCTT
GAACCTCTATTATTAGAATTGGAAAGAACTAAATCACCAGTCCTAATCGTTTCTCATCAA
GCCACTCTTCGTTGTCTCTATTCTTATCTCAAAGGTAGAGTTAAAGAAGAATGCCCCTTC
ATTAATATTCCATTACATACTTTAATTCAATTATCACCAAAAGCATAG
Length of full length seq. 1428