>SSG614 (SSG614Q) /CSM/SS/SSG6-A/SSG614Q.Seq.d/
AAAACTTTTAAAAATAAAATGATGGAAACAAATAATGAAAATAAAGAAAAATTAAAATTA
TATACTTGGGATGAAGTATCAAAACATAATCAAAAAAATGATTTATGGATTATAGTTGAT
GGTAAAGTTTATAATATTACAAAATGGGTACCATTACATCCAGGTGGTGAAGATATATTA
TTATTATCAGCAGGTAGAGATGCAACAAATTTATTTGAAAGTTATCATCCAATGACGGAT
AAACACTATTCCTTAATTAAACAATATGAAATTGGATATATATCATCATATGAACATCCA
AAATATGTTGAAAAAAGTGAATTCTATTCTACATTGAAACAACGTGTTAGAAAACATTTC
CAAACTTCATCACAAGATCCAAAAGTTTCAGTTGGAGTTTTCACAAGAATGGTGTTAATT
TATTTATTCCTATTTGTTACTTACTATTTATCACAATTCTCTACGGATAGATTTTGGTTA
AATTGTATATTCGCTGTTTTATATGGTGTTGCAAATTCGTTATTTGGATTACACACGATG
CATGACGCTTGCCACACAGCAATCACTCATAATCCAATGACTTGGAAAATATTGGGTGCA
ACATTTGATTTGTTCGCTGGTGCTTCATTCTATGCATGGTGTCATCAACATGTGATTGGG
CATCATTTATATACAAATGTAAGAAATGCAGATCCAGACTTGGGTCAAGGTGAAATTGAT
TTTCGTGTTGTTACACCATATCAAGCAAGATCATGGTACCATAAATATCAACATATTTAC
GCACCAATTCTATATGGAGTTTACGCTTTAAAATATCGTATTCAAGATCACGAAATCTTT
ACAAAGAAATCAAATGGTGCAATTAGATATTCACCAATATCAACGATTGATACTGCAATT
TTCATACTTGGTAAATTGGTTTTCATTATCTCTCGTTTCATACTCCCATTAATCTATAAT
CATTCATTCTCTCATTTAATTTGTTTCTTCCTAATCTCTGAATTGGTTTTAGGTTGGTAT
TTAGCCATTTCTTTTCAAGTTAGTCATGTAGTTGAAGATCTTCAATTCATGGCAACACCT
GAAATTTTCGATGGTGCTGATCACCCATTACCAACAACCTTCAATCAAGATTGGGCAATT
CTTCAAGTTAAAACTACTCAAGATTATGCTCAAGATTCAGTTTTAAGTACTTTCTTTTCT
GGTGGTTTAAATTTACAAGTTATTCATCATTGTTTCCCAACAATTGCTCAAGATTATTAC
CCACAAATTGTTCCAATTCTTAAAGAAGTTTGTAAAGAATATAATGTTACATATCATTAT
AAGCCAACATTTACTGAAGCAATAAAGTCTCATATCAACTATCTTTACAAAATGGGTAAT
GATCCAGACTATGTCAGAAAACCAGTAAACAAAAACGATTAATCAAACTGTATTATCTCT
TCTTAATTTCAAATTATTAGTTTATATCTCTTTATTTTATTTTTTTAAAAAAAATTGTTT
AGAAAAATTGAATCCAAGAAATAAAATCAATTTTATTGTATTTGTATTATCATTTAAAAA
AAAAA

KTFKNKMMETNNENKEKLKLYTWDEVSKHNQKNDLWIIVDGKVYNITKWVPLHPGGEDIL
LLSAGRDATNLFESYHPMTDKHYSLIKQYEIGYISSYEHPKYVEKSEFYSTLKQRVRKHF
QTSSQDPKVSVGVFTRMVLIYLFLFVTYYLSQFSTDRFWLNCIFAVLYGVANSLFGLHTM
HDACHTAITHNPMTWKILGATFDLFAGASFYAWCHQHVIGHHLYTNVRNADPDLGQGEID
FRVVTPYQARSWYHKYQHIYAPILYGVYALKYRIQDHEIFTKKSNGAIRYSPISTIDTAI
FILGKLVFIISRFILPLIYNHSFSHLICFFLISELVLGWYLAISFQVSHVVEDLQFMATP
EIFDGADHPLPTTFNQDWAILQVKTTQDYAQDSVLSTFFSGGLNLQVIHHCFPTIAQDYY
PQIVPILKEVCKEYNVTYHYKPTFTEAIKSHINYLYKMGNDPDYVRKPVNKND*snciis
s*fqiislylfilff*kklfrkieskk*nqfyciciii*kk

Frame A:
KTFKNKMMETNNENKEKLKLYTWDEVSKHNQKNDLWIIVDGKVYNITKWVPLHPGGEDIL
LLSAGRDATNLFESYHPMTDKHYSLIKQYEIGYISSYEHPKYVEKSEFYSTLKQRVRKHF
QTSSQDPKVSVGVFTRMVLIYLFLFVTYYLSQFSTDRFWLNCIFAVLYGVANSLFGLHTM
HDACHTAITHNPMTWKILGATFDLFAGASFYAWCHQHVIGHHLYTNVRNADPDLGQGEID
FRVVTPYQARSWYHKYQHIYAPILYGVYALKYRIQDHEIFTKKSNGAIRYSPISTIDTAI
FILGKLVFIISRFILPLIYNHSFSHLICFFLISELVLGWYLAISFQVSHVVEDLQFMATP
EIFDGADHPLPTTFNQDWAILQVKTTQDYAQDSVLSTFFSGGLNLQVIHHCFPTIAQDYY
PQIVPILKEVCKEYNVTYHYKPTFTEAIKSHINYLYKMGNDPDYVRKPVNKND*snciis
s*fqiislylfilff*kklfrkieskk*nqfyciciii*kk


Frame B:
kllkik*wkqimkikkn*nyilgmkyqniikkmiygl*lmvkfiilqngyhyiqvvkiyy
yyqqvemqqiylkviiq*rintip*lnnmkldiyhhmniqnmlkkvnsilh*nnvlenis
klhhkiqkfqlefsqewc*fiysyllltiyhnslridfg*ivyslfymvlqiryldytrc
mtlatqqsliiq*lgkywvqhlicslvlhsmhgvinm*lgiiyiqm*emqiqtwvkvkli
fvllhhikqdhgtininifthqfymeftl*nivfkitkslqrnqmvqldihqyqrlilqf
sylvnwfslslvsysh*siiihsli*fvss*slnwf*vgi*pflfklvm*lkifnswqhl
kfsmvlithyqqpsikigqffklkllkimlkiqf*vlsflvv*iyklfiivsqqllkiit
hklfqflkkfvknimlhiiisqhllkq*slistiftkwvmiqtmsenq*tktinqtvlsl
lnfkllvyislfyffkkncleklnprnkinfivfvlsfkkk


Frame C:
nf*k*ndgnk**k*rkikiiylg*sikt*skk*fmdys*w*sl*yykmgtitsrw*ryii
iisr*rcnkfi*klssndg*tlfln*ti*nwiyiii*tskic*kk*ilfyiettc*ktfp
nfitrsksfswsfhkngvnlfipicyllfitilyg*ilvklyircfiwcckfviwithda
*rlphsnhs*sndlenigcni*fvrwcfilcmvsstcdwasfiykckkcrsrlgsr*n*f
sccytisskimvp*istylrtnsiwslrfkisysrsrnlykeikwcn*iftnind*ycnf
htw*igfhylsfhtpinl*sfilsfnlflpnl*igfrlvfshffss*scs*rssihgnt*
nfrwc*spitnnlqsrlgnsss*nysrlcsrfsfkyflfwwfkftsysslfpnncsrllp
tncsns*rsl*ri*cyisl*aniy*snkvsyqlslqng**srlcqktskqkrliklyylf
lisny*fislyfiflkkiv*kn*iqeiksillylyyhlkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSG614 (SSG614Q) /CSM/SS/SSG6-A/SSG614Q.Seq.d/                       2880   0.0
SFJ773 (SFJ773Q) /CSM/SF/SFJ7-D/SFJ773Q.Seq.d/                       1348   0.0
SFF147 (SFF147Q) /CSM/SF/SFF1-B/SFF147Q.Seq.d/                       1330   0.0
CFB188 (CFB188Q) /CSM/CF/CFB1-D/CFB188Q.Seq.d/                       1277   0.0
SFG336 (SFG336Q) /CSM/SF/SFG3-B/SFG336Q.Seq.d/                       1162   0.0
VSD576 (VSD576Q) /CSM/VS/VSD5-D/VSD576Q.Seq.d/                       1051   0.0
SSD596 (SSD596Q) /CSM/SS/SSD5-D/SSD596Q.Seq.d/                        670   0.0
AFA237 (AFA237Q) /CSM/AF/AFA2-B/AFA237Q.Seq.d/                        577   e-163
VSF803 (VSF803Q) /CSM/VS/VSF8-A/VSF803Q.Seq.d/                        149   2e-34
VFO589 (VFO589Q) /CSM/VF/VFO5-D/VFO589Q.Seq.d/                        149   2e-34

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB022097|AB022097.1 Dictyostelium discoideum FadB mRNA for delta 5 fatty acid desaturase, complete cds.  2752  0.0  3
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   113  e-126  13
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.    90  1e-54  7
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.    90  1e-54  7
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.    90  1e-54  7
BM161245|BM161245.1 EST563768 PyBS Plasmodium yoelii yoelii cDNA clone PYCKC42 5' end, mRNA sequence.    50  0.086  1
BM161919|BM161919.1 EST564442 PyBS Plasmodium yoelii yoelii cDNA clone PYCKK71 5' end, mRNA sequence.    50  0.086  1
BM159966|BM159966.1 EST562489 PyBS Plasmodium yoelii yoelii cDNA clone PYCJL20 5' end, mRNA sequence.    50  0.086  1
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    36  0.12  9
AC091789|AC091789.5 Homo sapiens BAC clone RP11-496L12 from 7, complete sequence.    32  0.15  11

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   952   0.0
(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   674   0.0
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   565   e-159
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   361   4e-98
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   361   5e-98
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   338   3e-91
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   311   5e-83
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    296   2e-78
AM949596_1(AM949596|pid:none) Mantoniella squamata d5 gene for A...   268   4e-70
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   266   1e-69

 psg: 0.27  gvh: 0.18  alm: 0.25  top: 0.47  tms: 0.27  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 52.0 %: plasma membrane
	 28.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  4.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for SSG614 is pla

>SSG614Z.Seq
----------AATCTTTACAAAGAAATCAAATGGTGCAATTAGATATTCACCAATATCAA
CGATTGATACTGCAATTTTCATACTTGGTAAATTGGTTTTCATTATCTCTCGTTTCATAC
TCCCATTAATCTATAATCATTCATTCTCTCATTTAATTTGTTTCTTCCTAATCTCTGAAT
TGGTTTTAGGTTGGTATTTAGCCATTTCTTTTCAAGTTAGTCATGTAGTTGAAGATCTTC
AATTCATGGCAACACCTGAAATTTTCGATGGTGCTGATCACCCATTACCAACAACCTTCA
ATCAAGATTGGGCAATTCTTCAAGTTAAAACTACTCAAGATTATGCTCAAGATTCAGTTT
TAAGTACTTTCTTTTCTGGTGGTTTAAATTTACAAGTTATTCATCATTGTTTCCCAACAA
TTGCTCAAGATTATTACCCACAAATTGTTCCAATTCTTAAAGAAGTTTGTAAAGAATATA
ATGTTACATATCATTATAAGCCAACATTTACTGAAGCAATAAAGTCTCATATCAACTATC
TTTACAAAATGGGTAATGATCCAGACTATGTCAGAAAACCAGTAAACAAAAACGATTAAT
CAAACTGTATTATCTCTTCTTAATTTCAAATTATTAGTTTATATCTCTTTATTTTATTTT
TTTAAAAAAAATTGTTTAGAAAAATTGAATCCAAGAAATAAAATCAATTTTATTGTATTT
GTATTATCATTTAAAAAAAAAA

>SSG614E.Seq
AAAACTTTTAAAAATAAAATGATGGAAACAAATAATGAAAATAAAGAAAAATTAAAATTA
TATACTTGGGATGAAG
TATCAAAACATAATCAAAAAAATGATTTATGGATTATAGTTGA
TGGTAAAGTTTATAATATTACAAAATGGGTACC
ATTACATCCAGGTGGTGAAGATATAT
TATTATTATCAGCAGGTAGAGATGCAACAAATTTATTTGAAAGTTATCAT
CCAATGACG
GATAAACACTATTCCTTAATTAAACAATATGAAATTGGATATATATCATCATATGAACAT
CCAAAAT
ATGTTGAAAAAAGTGAATTCTATTCTACATTGAAACAACGTGTTAGAAAACA
TTTCCAAACTTCATCACAAGATCC
AAAAGTTTCAGTTGGAGTTTTCACAAGAATGGTGT
TAATTTATTTATTCCTATTTGTTACTTACTATTTATCACAA
TTCTCTACGGATAGATTT
TGGTTAAATTGTATATTCGCTGTTTTATATGGTGTTGCAAATTCGTTATTTGGATTAC
A
CACGATGCATGACGCTTGCCACACAGCAATCACTCATAATCCAATGACTTGGAAAATATT
GGGTGCAACATTTGA
TTTGTTCGCTGGTGCTTCATTCTATGCATGGTGTCATCAACATG
TGATTGGGCATCATTTATATACAAATGTAAGA
AATGCAGATCCAGACTTGGGTCAAGGT
GAAATTGATTTTCGTGTTGTTACACCATATCAAGCAAGATCATGGTACC
ATAAATATCA
ACATATTTACGCACCAATTCTATATGGAGTTTACGCTTTAAAATATCGTATTCAAGATCA
CGAAAT
CTTTACAAAGAAATCAAATGGTGCAATTAGATATTCACCAATATCAACGATTG
ATACTGCAATTTTCATACTTGGT
AAATTGGTTTTCATTATCTCTCGTTTCATACTCCCA
TTAATCTATAATCATTCATTCTCTCATTTAATTTGTTTCT
TCCTAATCTCTGAATTGGT
TTTAGGTTGGTATTTAGCCATTTCTTTTCAAGTTAGTCATGTAGTTGAAGATCTTCA
AT
TCATGGCAACACCTGAAATTTTCGATGGTGCTGATCACCCATTACCAACAACCTTCAATC
AAGATTGGGCAATT
CTTCAAGTTAAAACTACTCAAGATTATGCTCAAGATTCAGTTTTA
AGTACTTTCTTTTCTGGTGGTTTAAATTTAC
AAGTTATTCATCATTGTTTCCCAACAAT
TGCTCAAGATTATTACCCACAAATTGTTCCAATTCTTAAAGAAGTTTG
TAAAGAATATA
ATGTTACATATCATTATAAGCCAACATTTACTGAAGCAATAAAGTCTCATATCAACTATC
TTTAC
AAAATGGGTAATGATCCAGACTATGTCAGAAAACCAGTAAACAAAAACGATTAA
TCAAACTGTATTATCTCTTCTT
AATTTCAAATTATTAGTTTATATCTCTTTATTTTATT
TTTTTAAAAAAAATTGTTTAGAAAAATTGAATCCAAGAA
ATAAAATCAATTTTATTGTA
TTTGTATTATCATTTAAAAAAAAAA