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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB353
Library AF
(Link to library)
Clone ID AFB353
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15536-1
Original site URL
Representative seq. ID AFB353P
(Link to Original site)
Representative DNA sequence 
>AFB353 (AFB353Q) /CSM/AF/AFB3-C/AFB353Q.Seq.d/
ATTTTCATTTTTTATTTAATCATTTATAAATAAATATAAAATAAAAATATATTAAAAAAT
GTCAGAACCAGTAGTTGACAATGTTACAAATAAAGTTGAAAAGATGGAAGTAAAAGAAAA
AACTTCAGCACCACCAAAAGAGAAAAAAGAAAAGAAATCAAATAAAGTTCAATTAAAAAC
ACCAAAAGGTACTCAAGATTATAATCCAAGACAAATGACAATTCGTGAACAAGTCTTTGA
TGGTATTAAACAAGTTTTTAAAAGACATGGTGCTGTTACAATTGAAACACCAGTATTTGA
ATTAAAGGAAACATTAACAGGTAAATATGGTGAAGATTCAAAATTAATTTATGATTTACA
AGATCAAGGTGGTGAAATTTGTTCATTAAGATATGATTTAACAGTACCATTCGCACGTTA
TGTTGCAATGAATGGTGTATTGAATATAAAGAGATATCATATTGCACGTGTTTATCGTCG
TGATAATCCAATTATGACCAAAGGTAGATTTAGXXXXXXXXXXATTTAGAATGTTTTGGT
GTCACTCAAACATATCCTCTTTGATCTTTCATTGGCAAGAGGTTTGGATTACTACACTGG
TATCATCTATGAAGCAGTTTTAACAGGTCAAGATCGTGTTGGTTCCATCGCTGCTGGTGG
TCGTTACGATGGTTTGGTCGGTATGTATGGTAAAAAAGATGTACCAGCAGTTGGTTTCTC
AATTGGTATCGAACGTATCTTTACCATCTTGGAGGACGAATACAAAAAAGAGAATAAGAA
AATCAGAGAGAATGCAACTCAAGTTTTCGTTGTTCAAATGGAGAAAGATTTAATTAAAGA
ACGTCTTGCCATCGTCTCTGAACTTTGGAAAGCTGGTATCAACGCTGAATTCTCTTACAA
AGTTAATCCAAAACTTCCTGCTCAATTAAATACTGCCGATGAATCAAATATTCCACTCAT
CATCATCATTGGTAAATCTGAAGTTGAAACTAATAGTCTCTCTGTTAAAACTATGCACGA
TCGTAAACAAGTTTCAATTGAACGTTCAAATTTCACTGTTAAAATTAAAGAAATTTTATC
AACAATTCCAAAATAAATTAATTAATACATT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fsffi*sfinkykikiy*KMSEPVVDNVTNKVEKMEVKEKTSAPPKEKKEKKSNKVQLKT
PKGTQDYNPRQMTIREQVFDGIKQVFKRHGAVTIETPVFELKETLTGKYGEDSKLIYDLQ
DQGGEICSLRYDLTVPFARYVAMNGVLNIKRYHIARVYRRDNPIMTKGRF---

---FRMFWCHSNISSLIFHWQEVWITTLVSSMKQF*qvkivlvpsllvvvtmvwsvcmvk
kmyqqlvsqlvsnvslpswrtntkkrirksermqlkfslfkwrki*lknvlpsslnfgkl
vstlnsltkliqnflln*ilpmnqifhsssslvnlklklivsllklctivnkfqlnvqis
llklkkfyqqfqnklint
Translated Amino Acid sequence (All Frames) 
Frame A:
ififyliiyk*i*nknilknvrtss*qcyk*s*kdgskrknfsttkrekrkeik*ssikn
tkrysrl*sktndns*tsl*wy*tsf*ktwccyn*ntsi*ikgninr*iw*rfkinl*ft
rsrw*nlfiki*fnstirtlccnewcieykeisyctclss**snydqr*i*---

---i*nvlvslkhilfdlslargldyytgiiyeavltgqdrvgsiaaggrydglvgmygk
kdvpavgfsigieriftiledeykkenkkirenatqvfvvqmekdlikerlaivselwka
ginaefsykvnpklpaqlntadesnipliiiigksevetnslsvktmhdrkqvsiersnf
tvkikeilstipk*in*yi

Frame B:
fsffi*sfinkykikiy*KMSEPVVDNVTNKVEKMEVKEKTSAPPKEKKEKKSNKVQLKT
PKGTQDYNPRQMTIREQVFDGIKQVFKRHGAVTIETPVFELKETLTGKYGEDSKLIYDLQ
DQGGEICSLRYDLTVPFARYVAMNGVLNIKRYHIARVYRRDNPIMTKGRF---

---FRMFWCHSNISSLIFHWQEVWITTLVSSMKQF*qvkivlvpsllvvvtmvwsvcmvk
kmyqqlvsqlvsnvslpswrtntkkrirksermqlkfslfkwrki*lknvlpsslnfgkl
vstlnsltkliqnflln*ilpmnqifhsssslvnlklklivsllklctivnkfqlnvqis
llklkkfyqqfqnklint

Frame C:
fhflfnhl*inik*kyikkcqnq*ltmlqiklkrwk*kkklqhhqkrkkkrnqikfn*kh
qkvlkiiiqdk*qfvnkslmvlnkflkdmvllqlkhqyln*rkh*qvnmvkiqn*fmiyk
ikvvkfvh*dmi*qyhshvmlq*mvy*i*rdiilhvfivviiql*pkvdl---

---lecfgvtqtypl*sfigkrfgllhwyhl*ssfnrsrscwfhrcwwslrwfgryvw*k
rctsswflnwyrtylyhlggriqkre*enqrecnssfrcsngerfn*rtschrl*tlesw
yqr*illqs*sktscsikycr*ikysthhhhw*i*s*n**slc*nyars*tsfn*tfkfh
c*n*rnfinnskin*lih
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB353 (AFB353Q) /CSM/AF/AFB3-C/AFB353Q.Seq.d/                       1772   0.0
SSK761 (SSK761Q) /CSM/SS/SSK7-C/SSK761Q.Seq.d/                       1150   0.0
SSI792 (SSI792Q) /CSM/SS/SSI7-D/SSI792Q.Seq.d/                       1150   0.0
AFD186 (AFD186Q) /CSM/AF/AFD1-D/AFD186Q.Seq.d/                       1150   0.0
AFE848 (AFE848Q) /CSM/AF/AFE8-B/AFE848Q.Seq.d/                       1134   0.0
SFI440 (SFI440Q) /CSM/SF/SFI4-B/SFI440Q.Seq.d/                       1126   0.0
SFI340 (SFI340Q) /CSM/SF/SFI3-B/SFI340Q.Seq.d/                       1126   0.0
VSJ634 (VSJ634Q) /CSM/VS/VSJ6-B/VSJ634Q.Seq.d/                       1120   0.0
AFC220 (AFC220Q) /CSM/AF/AFC2-A/AFC220Q.Seq.d/                       1116   0.0
AFJ590 (AFJ590Q) /CSM/AF/AFJ5-D/AFJ590Q.Seq.d/                       1080   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.  1116  0.0  6
AU004124|AU004124.1 Bombyx mori cDNA, clone ws00954.              88  1e-15  2
BP119758|BP119758.1 Bombyx mori cDNA, clone:ceN-1281.             88  1e-15  2
AL423049|AL423049.1 T7 end of clone AZ0AA005G12 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    52  3e-13  4
AE014823|AE014823.1 Plasmodium falciparum 3D7 chromosome 14 section 8 of 13 of the complete sequence.    92  2e-12  7
AL049182|AL049182.3 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P5.    64  7e-11  4
AU001000|AU001000.1 Bombyx mori cDNA, clone fbf0885f.             60  1e-08  2
BI073474|BI073474.1 kt31c10.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to TR:Q22408 Q22408 T11G6.1 PROTEIN. [1] ;, mRNA sequence.    60  6e-05  1
AV383171|AV383171.1 Halocynthia roretzi cDNA clone:005A01_5, 5' end, expressed in fertilized egg.    46  1e-04  2
AQ023716|AQ023716.1 CpGR0123A Cryptosporidium parvum genomic random library Cryptosporidium parvum genomic clone CpGR0123A 5', genomic survey sequence.    52  4e-04  2
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86AS6) RecName: Full=Histidyl-tRNA synthetase, cytoplasmic;   ...   353   7e-96
BC076748_1(BC076748|pid:none) Xenopus laevis hypothetical protei...   194   6e-48
AK146922_1(AK146922|pid:none) Mus musculus 17 days embryo kidney...   191   3e-47
AK152329_1(AK152329|pid:none) Mus musculus bone marrow macrophag...   191   3e-47
BC020088_1(BC020088|pid:none) Mus musculus histidyl-tRNA synthet...   191   3e-47
AK011685_1(AK011685|pid:none) Mus musculus 10 days embryo whole ...   191   3e-47
AK088824_1(AK088824|pid:none) Mus musculus 2 days neonate thymus...   191   3e-47
BC097969_1(BC097969|pid:none) Rattus norvegicus dead end homolog...   191   3e-47
AK150081_1(AK150081|pid:none) Mus musculus bone marrow macrophag...   189   1e-46
AY995220_1(AY995220|pid:none) Homo sapiens histidyl-tRNA synthet...   189   1e-46
protein update 2009. 3.27
PSORT 

 psg: 0.75  gvh: 0.12  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for AFB353 is nuc
5' end seq. ID AFB353F
5' end seq. 
>AFB353F.Seq
ATTTTCATTTTTTATTTAATCATTTATAAATAAATATAAAATAAAAATATATTAAAAAAT
GTCAGAACCAGTAGTTGACAATGTTACAAATAAAGTTGAAAAGATGGAAGTAAAAGAAAA
AACTTCAGCACCACCAAAAGAGAAAAAAGAAAAGAAATCAAATAAAGTTCAATTAAAAAC
ACCAAAAGGTACTCAAGATTATAATCCAAGACAAATGACAATTCGTGAACAAGTCTTTGA
TGGTATTAAACAAGTTTTTAAAAGACATGGTGCTGTTACAATTGAAACACCAGTATTTGA
ATTAAAGGAAACATTAACAGGTAAATATGGTGAAGATTCAAAATTAATTTATGATTTACA
AGATCAAGGTGGTGAAATTTGTTCATTAAGATATGATTTAACAGTACCATTCGCACGTTA
TGTTGCAATGAATGGTGTATTGAATATAAAGAGATATCATATTGCACGTGTTTATCGTCG
TGATAATCCAATTATGACCAAAGGTAGATTTAG----------
Length of 5' end seq. 513
3' end seq. ID AFB353Z
3' end seq. 
>AFB353Z.Seq
----------ATTTAGAATGTTTTGGTGTCACTCAAACATATCCTCTTTGATCTTTCATT
GGCAAGAGGTTTGGATTACTACACTGGTATCATCTATGAAGCAGTTTTAACAGGTCAAGA
TCGTGTTGGTTCCATCGCTGCTGGTGGTCGTTACGATGGTTTGGTCGGTATGTATGGTAA
AAAAGATGTACCAGCAGTTGGTTTCTCAATTGGTATCGAACGTATCTTTACCATCTTGGA
GGACGAATACAAAAAAGAGAATAAGAAAATCAGAGAGAATGCAACTCAAGTTTTCGTTGT
TCAAATGGAGAAAGATTTAATTAAAGAACGTCTTGCCATCGTCTCTGAACTTTGGAAAGC
TGGTATCAACGCTGAATTCTCTTACAAAGTTAATCCAAAACTTCCTGCTCAATTAAATAC
TGCCGATGAATCAAATATTCCACTCATCATCATCATTGGTAAATCTGAAGTTGAAACTAA
TAGTCTCTCTGTTAAAACTATGCACGATCGTAAACAAGTTTCAATTGAACGTTCAAATTT
CACTGTTAAAATTAAAGAAATTTTATCAACAATTCCAAAATAAATTAATTAATACATT
Length of 3' end seq. 588
Connected seq. ID AFB353P
Connected seq. 
>AFB353P.Seq
ATTTTCATTTTTTATTTAATCATTTATAAATAAATATAAAATAAAAATATATTAAAAAAT
GTCAGAACCAGTAGTTGACAATGTTACAAATAAAGTTGAAAAGATGGAAGTAAAAGAAAA
AACTTCAGCACCACCAAAAGAGAAAAAAGAAAAGAAATCAAATAAAGTTCAATTAAAAAC
ACCAAAAGGTACTCAAGATTATAATCCAAGACAAATGACAATTCGTGAACAAGTCTTTGA
TGGTATTAAACAAGTTTTTAAAAGACATGGTGCTGTTACAATTGAAACACCAGTATTTGA
ATTAAAGGAAACATTAACAGGTAAATATGGTGAAGATTCAAAATTAATTTATGATTTACA
AGATCAAGGTGGTGAAATTTGTTCATTAAGATATGATTTAACAGTACCATTCGCACGTTA
TGTTGCAATGAATGGTGTATTGAATATAAAGAGATATCATATTGCACGTGTTTATCGTCG
TGATAATCCAATTATGACCAAAGGTAGATTTAG----------ATTTAGAATGTTTTGGT
GTCACTCAAACATATCCTCTTTGATCTTTCATTGGCAAGAGGTTTGGATTACTACACTGG
TATCATCTATGAAGCAGTTTTAACAGGTCAAGATCGTGTTGGTTCCATCGCTGCTGGTGG
TCGTTACGATGGTTTGGTCGGTATGTATGGTAAAAAAGATGTACCAGCAGTTGGTTTCTC
AATTGGTATCGAACGTATCTTTACCATCTTGGAGGACGAATACAAAAAAGAGAATAAGAA
AATCAGAGAGAATGCAACTCAAGTTTTCGTTGTTCAAATGGAGAAAGATTTAATTAAAGA
ACGTCTTGCCATCGTCTCTGAACTTTGGAAAGCTGGTATCAACGCTGAATTCTCTTACAA
AGTTAATCCAAAACTTCCTGCTCAATTAAATACTGCCGATGAATCAAATATTCCACTCAT
CATCATCATTGGTAAATCTGAAGTTGAAACTAATAGTCTCTCTGTTAAAACTATGCACGA
TCGTAAACAAGTTTCAATTGAACGTTCAAATTTCACTGTTAAAATTAAAGAAATTTTATC
AACAATTCCAAAATAAATTAATTAATACATT
Length of connected seq. 1101
Full length Seq ID -
Full length Seq. -
Length of full length seq. -