>SSG185 (SSG185Q) /CSM/SS/SSG1-D/SSG185Q.Seq.d/
TACAAATAGAAATGGTGAATTTGGTACAGTTAGTGCAGGACATGTTGATGAACTTACACA
ATTTAATAGTTCAACACTTAAATTTAAAGAGATCATTTGTGCACAACAACATACTATGTT
TATTGGTGTTGATGGTAAACTTTATGGTAGTGGTAAACCAGAGTTATTGGGAATGTCAAC
TGATGAAGCAAAGAAATCACCTACATTAATACCAGCATTATCACAACATAAGATTGCATT
ATATGCAACCAGTAATAAACATTCATTCGCTGTTACTGAGGATGGTATCTTATTTGGTTG
GGGTAAAAGTGACCTTTTAGGTATTGGTAGTTTTGTAAAAGTAAAGAAAATCACTGTCAC
AACTAAAGAACCAATTGTAATCGATACATGTTCTGCTTTCTTATCTTTTAGAATTAAAAG
TATTGCATGTGGTGAAGAACATACTATTGCTTTATTAGAAACTGGTGATGTTTATGGTTG
GGGTCGTGGTGATGAAGGTCAATTAGGACATGGTATTAAAGAGAGTAGAGTAACACCAAT
TTTAATTGAATCAGTTAAATCATTAAATATTATTAGTGTTCAATGTGGTTCATATAATTC
ATCAGTATTAACAACAGATAAGGAATGTTATATTTGGGGTCAXXXXXXXXXXTTGGTGAA
GAATTAATAATGACACCACAACCATTCTGTCCAGGTGGTTCAAGACAACCATTAAAAGTT
CAACAAATTTCACAAAATGGTTTCTATAATATAGTGGTTACATTAAGTGGTGATATCTAT
CATTTTGGTATTGATTATAAATTATTTACAAAATCAGCTTGGATTCCAACCCTTTTGAAA
ATTGATGATCCATTATTAATGGGTAAAAGAGTTACACAAGTTGCAGCTTCTGCTGCTGGT
TTTTATATGATCGTTTATGATGGTACTCCAATTTATTATCCAGATATTGATTATTCAAGA
AAAGGTGCTAAACCAACTAAAACTTTAGCACCTCTACCTGAATCACAATATTCAAAGAAT
AAAGACGCCTTATTCGGTGGTAATAAACCTACAAATTCAACATATTTAAATAAAGAAAGA
AATAAAAATAATCCAACCACCACAACTACCACAACTTCAACTTCAAATAACTCAAGAAAT
CATCGTGATAATGATGAAGATGTTTTATAAATTAAATAATAAATAATAAATAATAATAAT
ATTAAAAATAGAAAAA

TNRNGEFGTVSAGHVDELTQFNSSTLKFKEIICAQQHTMFIGVDGKLYGSGKPELLGMST
DEAKKSPTLIPALSQHKIALYATSNKHSFAVTEDGILFGWGKSDLLGIGSFVKVKKITVT
TKEPIVIDTCSAFLSFRIKSIACGEEHTIALLETGDVYGWGRGDEGQLGHGIKESRVTPI
LIESVKSLNIISVQCGSYNSSVLTTDKECYIWG---

---GEELIMTPQPFCPGGSRQPLKVQQISQNGFYNIVVTLSGDIYHFGIDYKLFTKSAWI
PTLLKIDDPLLMGKRVTQVAASAAGFYMIVYDGTPIYYPDIDYSRKGAKPTKTLAPLPES
QYSKNKDALFGGNKPTNSTYLNKERNKNNPTTTTTTTSTSNNSRNHRDNDEDVL*ik**i
innnniknrk

Frame A:
yk*kw*iwys*crtc**tyti**fnt*i*rdhlctttyyvywc*w*tlw*w*trvignvn
**skeityintsiitt*dciicnq**tfircy*gwyliwlg*k*pfryw*fckskenhch
n*rtncnrymfcflif*n*kycmw*rtyycfirnw*clwlgsw**rsirtwy*re*sntn
fn*is*iikyy*csmwfi*fisinnr*gmlylgs---

---lvkn***hhnhsvqvvqdnh*kfnkfhkmvsii*wlh*vvisiilvliinylqnqlg
fqpf*klmihy*wvkelhklqllllvfi*sfmmvlqfiiqiliiqekvlnqlkl*hlyln
hniqriktpysvvinlqiqhi*ikkeikiiqppqlpqlqlqitqeiivimmkmfyklnnk
**iiiilkiek

Frame B:
TNRNGEFGTVSAGHVDELTQFNSSTLKFKEIICAQQHTMFIGVDGKLYGSGKPELLGMST
DEAKKSPTLIPALSQHKIALYATSNKHSFAVTEDGILFGWGKSDLLGIGSFVKVKKITVT
TKEPIVIDTCSAFLSFRIKSIACGEEHTIALLETGDVYGWGRGDEGQLGHGIKESRVTPI
LIESVKSLNIISVQCGSYNSSVLTTDKECYIWG---

---w*rinndtttilsrwfkttiksstnftkwfl*ysgyikw*ylsfwy*l*iiykisld
snpfen**siing*ksytscsfccwflydrl*wysnllsry*lfkkrc*tn*nfstst*i
tifke*rrlirw**tykfnifk*rkk*k*snhhnyhnfnfk*lkkss****rcfin*iin
nk***y*k*k

Frame C:
qiemvnlvqlvqdmlmnlhnlivqhlnlkrsfvhnnilcllvlmvnfmvvvnqsywecql
mkqrnhlh*yqhyhnirlhymqpvinihslllrmvsylvgvkvtf*vlvvl*k*rkslsq
lknql*sihvllsyllelkvlhvvknillly*klvmfmvgvvvmkvn*dmvlkrve*hqf
*lnqlnh*illvfnvvhiihqy*qqirnvifgv---

---GEELIMTPQPFCPGGSRQPLKVQQISQNGFYNIVVTLSGDIYHFGIDYKLFTKSAWI
PTLLKIDDPLLMGKRVTQVAASAAGFYMIVYDGTPIYYPDIDYSRKGAKPTKTLAPLPES
QYSKNKDALFGGNKPTNSTYLNKERNKNNPTTTTTTTSTSNNSRNHRDNDEDVL*ik**i
innnniknrk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSG185 (SSG185Q) /CSM/SS/SSG1-D/SSG185Q.Seq.d/                       2250   0.0
VFK757 (VFK757Q) /CSM/VF/VFK7-C/VFK757Q.Seq.d/                       2070   0.0
SLK612 (SLK612Q) /CSM/SL/SLK6-A/SLK612Q.Seq.d/                         44   0.005
SFK689 (SFK689Q) /CSM/SF/SFK6-D/SFK689Q.Seq.d/                         44   0.005
SSE868 (SSE868Q) /CSM/SS/SSE8-C/SSE868Q.Seq.d/                         42   0.020
CHI439 (CHI439Q) /CSM/CH/CHI4-B/CHI439Q.Seq.d/                         42   0.020
VHH223 (VHH223Q) /CSM/VH/VHH2-A/VHH223Q.Seq.d/                         40   0.078
VFK213 (VFK213Q) /CSM/VF/VFK2-A/VFK213Q.Seq.d/                         40   0.078
VFK176 (VFK176Q) /CSM/VF/VFK1-D/VFK176Q.Seq.d/                         40   0.078
VFH729 (VFH729Q) /CSM/VF/VFH7-B/VFH729Q.Seq.d/                         40   0.078

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117397|AC117397.2 Homo sapiens 3 BAC RP11-92D20 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38  0.001  4
AC068544|AC068544.7 Homo sapiens BAC clone RP11-369J9 from 2, complete sequence.    52  0.016  1
AE014828|AE014828.1 Plasmodium falciparum 3D7 chromosome 14 section 13 of 13 of the complete sequence.    38  0.059  12
AC099846|AC099846.1 Homo sapiens chromosome 18 clone RP11-815E12 map 18, LOW-PASS SEQUENCE SAMPLING.    50  0.065  1
AL139227|AL139227.2 Human chromosome 16 DNA sequence *** IN PROGRESS *** BAC R-615I2 of  library RPCI-11 from chromosome 16 of Homo sapiens (Human).    50  0.065  1
AX646661|AX646661.1 Sequence 853 from Patent EP1270724.           50  0.065  1
AB065942|AB065942.1 Homo sapiens gene for seven transmembrane helix receptor, complete cds, isolate:CBRC7TM_505.    50  0.065  1
AC126773|AC126773.2 Homo sapiens chromosome 16 clone RP11-615I2, complete sequence.    50  0.065  1
AC111880|AC111880.5 Rattus norvegicus clone CH230-25K13, WORKING DRAFT SEQUENCE, 3 unordered pieces.    34  0.073  10
AC091953|AC091953.3 Homo sapiens chromosome 5 clone RP11-417J1, complete sequence.    40  0.080  7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT040520_1(BT040520|pid:none) Zea mays full-length cDNA clone ZM...    93   2e-17
EU960715_1(EU960715|pid:none) Zea mays clone 227856 HECT domain ...    93   2e-17
AK100857_1(AK100857|pid:none) Oryza sativa Japonica Group cDNA c...    91   6e-17
AK069920_1(AK069920|pid:none) Oryza sativa Japonica Group cDNA c...    91   8e-17
AE014298_3135(AE014298|pid:none) Drosophila melanogaster chromos...    90   1e-16
AK220338_1(AK220338|pid:none) Mus musculus mRNA for mKIAA0393 pr...    89   3e-16
AF061529_1(AF061529|pid:none) Mus musculus rjs (rjs) mRNA, compl...    89   3e-16
AB007646_6(AB007646|pid:none) Arabidopsis thaliana genomic DNA, ...    89   3e-16
(Q7TPR5) RecName: Full=Probable E3 ubiquitin-protein ligase HERC...    89   3e-16
(Q86YY7) RecName: Full=Probable E3 ubiquitin-protein ligase HERC...    89   4e-16

 psg: 0.84  gvh: 0.39  alm: 0.39  top: 0.43  tms: 0.00  mit: 0.21  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for SSG185 is nuc

>SSG185F.Seq
TACAAATAGAAATGGTGAATTTGGTACAGTTAGTGCAGGACATGTTGATGAACTTACACA
ATTTAATAGTTCAACACTTAAATTTAAAGAGATCATTTGTGCACAACAACATACTATGTT
TATTGGTGTTGATGGTAAACTTTATGGTAGTGGTAAACCAGAGTTATTGGGAATGTCAAC
TGATGAAGCAAAGAAATCACCTACATTAATACCAGCATTATCACAACATAAGATTGCATT
ATATGCAACCAGTAATAAACATTCATTCGCTGTTACTGAGGATGGTATCTTATTTGGTTG
GGGTAAAAGTGACCTTTTAGGTATTGGTAGTTTTGTAAAAGTAAAGAAAATCACTGTCAC
AACTAAAGAACCAATTGTAATCGATACATGTTCTGCTTTCTTATCTTTTAGAATTAAAAG
TATTGCATGTGGTGAAGAACATACTATTGCTTTATTAGAAACTGGTGATGTTTATGGTTG
GGGTCGTGGTGATGAAGGTCAATTAGGACATGGTATTAAAGAGAGTAGAGTAACACCAAT
TTTAATTGAATCAGTTAAATCATTAAATATTATTAGTGTTCAATGTGGTTCATATAATTC
ATCAGTATTAACAACAGATAAGGAATGTTATATTTGGGGTCA----------

>SSG185Z.Seq
----------TTGGTGAAGAATTAATAATGACACCACAACCATTCTGTCCAGGTGGTTCA
AGACAACCATTAAAAGTTCAACAAATTTCACAAAATGGTTTCTATAATATAGTGGTTACA
TTAAGTGGTGATATCTATCATTTTGGTATTGATTATAAATTATTTACAAAATCAGCTTGG
ATTCCAACCCTTTTGAAAATTGATGATCCATTATTAATGGGTAAAAGAGTTACACAAGTT
GCAGCTTCTGCTGCTGGTTTTTATATGATCGTTTATGATGGTACTCCAATTTATTATCCA
GATATTGATTATTCAAGAAAAGGTGCTAAACCAACTAAAACTTTAGCACCTCTACCTGAA
TCACAATATTCAAAGAATAAAGACGCCTTATTCGGTGGTAATAAACCTACAAATTCAACA
TATTTAAATAAAGAAAGAAATAAAAATAATCCAACCACCACAACTACCACAACTTCAACT
TCAAATAACTCAAGAAATCATCGTGATAATGATGAAGATGTTTTATAAATTAAATAATAA
ATAATAAATAATAATAATATTAAAAATAGAAAAA

>SSG185P.Seq
TACAAATAGAAATGGTGAATTTGGTACAGTTAGTGCAGGACATGTTGATGAACTTACACA
ATTTAATAGTTCAACACTTAAATTTAAAGAGATCATTTGTGCACAACAACATACTATGTT
TATTGGTGTTGATGGTAAACTTTATGGTAGTGGTAAACCAGAGTTATTGGGAATGTCAAC
TGATGAAGCAAAGAAATCACCTACATTAATACCAGCATTATCACAACATAAGATTGCATT
ATATGCAACCAGTAATAAACATTCATTCGCTGTTACTGAGGATGGTATCTTATTTGGTTG
GGGTAAAAGTGACCTTTTAGGTATTGGTAGTTTTGTAAAAGTAAAGAAAATCACTGTCAC
AACTAAAGAACCAATTGTAATCGATACATGTTCTGCTTTCTTATCTTTTAGAATTAAAAG
TATTGCATGTGGTGAAGAACATACTATTGCTTTATTAGAAACTGGTGATGTTTATGGTTG
GGGTCGTGGTGATGAAGGTCAATTAGGACATGGTATTAAAGAGAGTAGAGTAACACCAAT
TTTAATTGAATCAGTTAAATCATTAAATATTATTAGTGTTCAATGTGGTTCATATAATTC
ATCAGTATTAACAACAGATAAGGAATGTTATATTTGGGGTCA----------TTGGTGAA
GAATTAATAATGACACCACAACCATTCTGTCCAGGTGGTTCAAGACAACCATTAAAAGTT
CAACAAATTTCACAAAATGGTTTCTATAATATAGTGGTTACATTAAGTGGTGATATCTAT
CATTTTGGTATTGATTATAAATTATTTACAAAATCAGCTTGGATTCCAACCCTTTTGAAA
ATTGATGATCCATTATTAATGGGTAAAAGAGTTACACAAGTTGCAGCTTCTGCTGCTGGT
TTTTATATGATCGTTTATGATGGTACTCCAATTTATTATCCAGATATTGATTATTCAAGA
AAAGGTGCTAAACCAACTAAAACTTTAGCACCTCTACCTGAATCACAATATTCAAAGAAT
AAAGACGCCTTATTCGGTGGTAATAAACCTACAAATTCAACATATTTAAATAAAGAAAGA
AATAAAAATAATCCAACCACCACAACTACCACAACTTCAACTTCAAATAACTCAAGAAAT
CATCGTGATAATGATGAAGATGTTTTATAAATTAAATAATAAATAATAAATAATAATAAT
ATTAAAAATAGAAAAA