>SSG144 (SSG144Q) /CSM/SS/SSG1-B/SSG144Q.Seq.d/
ATGAGTACCACCACCTGATGCTAATAATCCACGTTGATGTGGACTCCATGCAATAGCTTT
AACTGCTGCATAATGAAATTTAAATTGATAAAGAGGTTTACTTGTATTTGTTGTTGTTGT
TGTTGTGAGATAGAAGAGGTATTTGATGATGGTGGTGGTGGTGGTGGTTGATGTTGTTGT
TGAGGTTGTTGAGTCATTGAATGATCCCAAACATTTAATAAATTATCATTACCACCAGAT
GCTAATTGTTGACCATCAGGAGACCATTTTAAACCACAAATTTCATGCCTATGACCAACT
AATCTATTTGTGTAATTATTACTACAATCACGTACATCATGATGTAGAATCACTTTATCC
TTACCACCACTACTCAGTATATGATTATTCCAAGCCAAAGCGTTCACTCTTGTATTGTGA
CCTTGTAATTCTCTAATCTTTTTCTTTTTATTTACATCCCAAATCGAGACTACACCATCA
GTACCACCAATTGCCAAATGAATACCACCACGTTGTATCCAACCAACTGATGAAACTGGT
TGACCTGATTCCATTTCACAAAGTTTTGAAACTTGGCTTGTAGTTGCATTCCAAAGATAA
ACACTAGTATCCAAACCTACAGCTAAAATATTATGTGAACTCCAATCAATTAAATTTAAA
TAAAAGTCATCTTTTATCATTGGAGCAAAAAAAGCAAAAAAAAAAAAAAA

*vpppdannpr*cglhaialtaa**nln**rgllvfvvvvvvr*KRYLMMVVVVVVDVVV
EVVESLNDPKHLINYHYHQMLIVDHQETILNHKFHAYDQLIYLCNYYYNHVHHDVESLYP
YHHYSVYDYSKPKRSLLYCDLVIL*sfsfylhpksrlhhqyhqlpneyhhvvsnqlmklv
dlipfhkvlklgl*lhskdkh*ypnlqlkyyvnsnqlnlnkshllsleqkkqkkkk

Frame A:
msttt*c**stlmwtpcnsfnccimkfklikrftcicccccceieevfddgggggg*ccc
*gc*vie*sqtfnklslppdanc*psgdhfkpqiscl*ptnlfv*lllqsrts*critls
lppllsi*lfqakaftlvl*pcnsliffflftsqiettpsvppiak*ipprciqptdetg
*pdsisqsfetwlvvafqr*tlvskptakilcelqsikfk*kssfiigakkakkkk


Frame B:
*vpppdannpr*cglhaialtaa**nln**rgllvfvvvvvvr*KRYLMMVVVVVVDVVV
EVVESLNDPKHLINYHYHQMLIVDHQETILNHKFHAYDQLIYLCNYYYNHVHHDVESLYP
YHHYSVYDYSKPKRSLLYCDLVIL*sfsfylhpksrlhhqyhqlpneyhhvvsnqlmklv
dlipfhkvlklgl*lhskdkh*ypnlqlkyyvnsnqlnlnkshllsleqkkqkkkk


Frame C:
eyhhlmliihvdvdsmq*l*llhnei*idkevylyllllll*drrgi**wwwwwwlmlll
rllsh*mipni**iiittrc*lltirrpf*ttnfmpmtn*sicviittityimm*nhfil
ttttqymiipsqsvhscivtl*fsnlflfiyipnrdytisttncqmntttlyptn**nwl
t*fhftkf*nlacscipkintsiqtys*nim*tpin*i*ikvifyhwskkskkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSG144 (SSG144Q) /CSM/SS/SSG1-B/SSG144Q.Seq.d/                       1027   0.0
AFM131 (AFM131Q) /CSM/AF/AFM1-B/AFM131Q.Seq.d/                       1005   0.0
VSF565 (VSF565Q) /CSM/VS/VSF5-C/VSF565Q.Seq.d/                        987   0.0
SFJ337 (SFJ337Q) /CSM/SF/SFJ3-B/SFJ337Q.Seq.d/                        924   0.0
SSG240 (SSG240Q) /CSM/SS/SSG2-B/SSG240Q.Seq.d/                        906   0.0
SLK161 (SLK161Q) /CSM/SL/SLK1-C/SLK161Q.Seq.d/                        494   e-138
SLK439 (SLK439Q) /CSM/SL/SLK4-B/SLK439Q.Seq.d/                        422   e-117
SSL658 (SSL658Q) /CSM/SS/SSL6-C/SSL658Q.Seq.d/                        188   8e-47
VHN609 (VHN609Q) /CSM/VH/VHN6-A/VHN609Q.Seq.d/                         44   0.003
VHN355 (VHN355Q) /CSM/VH/VHN3-C/VHN355Q.Seq.d/                         44   0.003

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ566406|AJ566406.1 Paramecium tetraurelia macronuclear nd6 gene and fizzy1 gene for  fizzy related protein.    50  2e-07  2
BI451393|BI451393.1 kx23f12.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to TR:Q09649 Q09649 HYPOTHETICAL 76.4 KD PROTEIN ZK1307.6 IN CHROMOSOME II. [1] ;, mRNA sequence.    46  0.002  2
AL448561|AL448561.2 Paramecium tetraurelia sequence M21C01u of the end of plasmid  PT021E01; contig/duplicate sequence reads: M21C01u M21B01u.  Similarity to WD-REPEAT CELL CYCLE REGULATORY PROTEIN.    34  0.003  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    40  0.079  10
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38  0.12  8
AC120001|AC120001.3 Mus musculus clone RP24-143B14, WORKING DRAFT SEQUENCE, 12 unordered pieces.    44  0.13  2
AP001568|AP001568.2 Homo sapiens genomic DNA, chromosome 18q22 clone:RP11-850A23, WORKING DRAFT SEQUENCE, 18 unordered pieces.    44  0.14  2
AX307658|AX307658.1 Sequence 643 from Patent WO0190366.           40  0.15  2
AC115053|AC115053.4 Mus musculus clone RP24-341E20, WORKING DRAFT SEQUENCE, 4 ordered pieces.    48  0.15  1
X59428|X59428.1 S.cerevisiae cdc20 gene.                          40  0.36  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC098695_13(AC098695|pid:none) Oryza sativa (japonica cultivar-g...   206   6e-60
AK070642_1(AK070642|pid:none) Oryza sativa Japonica Group cDNA c...   206   6e-60
CP001327_500(CP001327|pid:none) Micromonas sp. RCC299 chromosome...   199   3e-59
BT069299_1(BT069299|pid:none) Zea mays full-length cDNA clone ZM...   201   2e-58
CR954213_250(CR954213|pid:none) Ostreococcus tauri strain OTTH05...   199   4e-58
DQ059035_1(DQ059035|pid:none) Lotus corniculatus var. japonicus ...   200   5e-58
BT071264_1(BT071264|pid:none) Picea sitchensis clone WS02816_B18...   193   1e-57
DQ059036_1(DQ059036|pid:none) Pisum sativum cell cycle switch pr...   196   6e-57
AF079404_1(AF079404|pid:none) Medicago sativa subsp. X varia cel...   196   6e-57
AM920435_696(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   187   9e-57

 psg: 0.99  gvh: 0.48  alm: 0.18  top: 0.17  tms: 0.07  mit: 0.26  mip: 0.02
 nuc: 0.03  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 1.67  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: mitochondrial
	 16.0 %: Golgi
	 12.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for SSG144 is mit

>SSG144F.Seq
ATGAGTACCACCACCTGATGCTAATAATCCACGTTGATGTGGACTCCATGCAATAGCTTT
AACTGCTGCATAATGAAATTTAAATTGATAAAGAGGTTTACTTGTATTTTGTTGTTGTTG
TTGTTGTGAGATAGAAGAGGTATTTGATGATGGTGGTGGTGGTGGTGGTTGATGTTGTTG
TTGAGGTTGTTGAGTCATTGAATGATCCCAAACATTTAATAAATTATCATTACCACCAGA
TGCTAATTGTTGACCATCAGGAGACCATTTTAAACCACAAATTTCATGCCTATGACCAAC
TAATCTATTTGTGTAATTATTACTACAATCACGTACATCATGATGTAGAATCACTTTATC
CTTACCACCACTACTCAGTATATGATTATTCCAAGCCAAAGCGTTCACTCTTGTATTGTG
ACCTTGTAATTCTCTAATCTTTTTCTTTTTATTTACATCCCAAATCGAGACTACACCATC
AGTACCACCAATTGCCAAATGAATACCACCACGTTGTATCCAACCAACTGATGAAACTGG
TTGACCTGATTCCATTTCACAAAGTTTTGAAACTTGGCTTGTAGTTGCATTCCAAAGATA
AACACTAGTATCCAAACCTACAGCTAAAATATTATGTGAACTCCAATCAATTAAATTTAA
ATAAAAGTCATCTTTTATCATTGGAGCAAAAAA----------

>SSG144Z.Seq
----------TGTTGTTGTTGTTGTTGTGAGATAGAAGAGGTATTTGATGATGGTGGTGG
TGGTGGTGGTTGATGTTGTTGTTGAGGTTGTTGAGTCATTGAATGATCCCAAACATTTAA
TAAATTATCATTACCACCAGATGCTAATTGTTGACCATCAGGAGACCATTTTAAACCACA
AATTTCATGCCTATGACCAACTAATCTATTTGTGTAATTATTACTACAATCACGTACATC
ATGATGTAGAATCACTTTATCCTTACCACCACTACTCAGTATATGATTATTCCAAGCCAA
AGCGTTCACTCTTGTATTGTGACCTTGTAATTCTCTAATCTTTTTCTTTTTATTTACATC
CCAAATCGAGACTACACCATCAGTACCACCAATTGCCAAATGAATACCACCACGTTGTAT
CCAACCAACTGATGAAACTGGTTGACCTGATTCCATTTCACAAAGTTTTGAAACTTGGCT
TGTAGTTGCATTCCAAAGATAAACACTAGTATCCAAACCTACAGCTAAAATATTATGTGA
ACTCCAATCAATTAAATTTAAATAAAAGTCATCTTTTATCATTGGAGCAAAAAAAAAAAA
AAAAAAAAAAAAA

>SSG144P.Seq
ATGAGTACCACCACCTGATGCTAATAATCCACGTTGATGTGGACTCCATGCAATAGCTTT
AACTGCTGCATAATGAAATTTAAATTGATAAAGAGGTTTACTTGTATTTTGTTGTTGTTG
TTGTTGTGAGATAGAAGAGGTATTTGATGATGGTGGTGGTGGTGGTGGTTGATGTTGTTG
TTGAGGTTGTTGAGTCATTGAATGATCCCAAACATTTAATAAATTATCATTACCACCAGA
TGCTAATTGTTGACCATCAGGAGACCATTTTAAACCACAAATTTCATGCCTATGACCAAC
TAATCTATTTGTGTAATTATTACTACAATCACGTACATCATGATGTAGAATCACTTTATC
CTTACCACCACTACTCAGTATATGATTATTCCAAGCCAAAGCGTTCACTCTTGTATTGTG
ACCTTGTAATTCTCTAATCTTTTTCTTTTTATTTACATCCCAAATCGAGACTACACCATC
AGTACCACCAATTGCCAAATGAATACCACCACGTTGTATCCAACCAACTGATGAAACTGG
TTGACCTGATTCCATTTCACAAAGTTTTGAAACTTGGCTTGTAGTTGCATTCCAAAGATA
AACACTAGTATCCAAACCTACAGCTAAAATATTATGTGAACTCCAATCAATTAAATTTAA
ATAAAAGTCATCTTTTATCATTGGAGCAAAAAA----------TGTTGTTGTTGTTGTTG
TGAGATAGAAGAGGTATTTGATGATGGTGGTGGTGGTGGTGGTTGATGTTGTTGTTGAGG
TTGTTGAGTCATTGAATGATCCCAAACATTTAATAAATTATCATTACCACCAGATGCTAA
TTGTTGACCATCAGGAGACCATTTTAAACCACAAATTTCATGCCTATGACCAACTAATCT
ATTTGTGTAATTATTACTACAATCACGTACATCATGATGTAGAATCACTTTATCCTTACC
ACCACTACTCAGTATATGATTATTCCAAGCCAAAGCGTTCACTCTTGTATTGTGACCTTG
TAATTCTCTAATCTTTTTCTTTTTATTTACATCCCAAATCGAGACTACACCATCAGTACC
ACCAATTGCCAAATGAATACCACCACGTTGTATCCAACCAACTGATGAAACTGGTTGACC
TGATTCCATTTCACAAAGTTTTGAAACTTGGCTTGTAGTTGCATTCCAAAGATAAACACT
AGTATCCAAACCTACAGCTAAAATATTATGTGAACTCCAATCAATTAAATTTAAATAAAA
GTCATCTTTTATCATTGGAGCAAAAAAAAAAAAAAAAAAAAAAAAA

>SSG144E.Seq
ATGAGTACCACCACCTGATGCTAATAATCCACGTTGATGTGGACTCCATGCAATAGCTTT
AACTGCTGCATAATGAAATTTAAATTGATAAAGAGGTTTACTTGTATTTGTTGTTGTTGT
TGTTGTGAGATAGAAGAGGTATTTGATGATGGTGGTGGTGGTGGTGGTTGATGTTGTTGT
TGAGGTTGTTGAGTCATTGAATGATCCCAAACATTTAATAAATTATCATTACCACCAGAT
GCTAATTGTTGACCATCAGGAGACCATTTTAAACCACAAATTTCATGCCTATGACCAACT
AATCTATTTGTGTAATTATTACTACAATCACGTACATCATGATGTAGAATCACTTTATCC
TTACCACCACTACTCAGTATATGATTATTCCAAGCCAAAGCGTTCACTCTTGTATTGTGA
CCTTGTAATTCTCTAATCTTTTTCTTTTTATTTACATCCCAAATCGAGACTACACCATCA
GTACCACCAATTGCCAAATGAATACCACCACGTTGTATCCAACCAACTGATGAAACTGGT
TGACCTGATTCCATTTCACAAAGTTTTGAAACTTGGCTTGTAGTTGCATTCCAAAGATAA
ACACTAGTATCCAAACCTACAGCTAAAATATTATGTGAACTCCAATCAATTAAATTTAAA
TAAAAGTCATCTTTTATCATTGGAGCAAAAAAAGCAAAAAAAAAAAAAAA