>AFL314 (AFL314Q) /CSM/AF/AFL3-A/AFL314Q.Seq.d/
XXXXXXXXXXAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTT
CCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAA
ACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCT
CCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTT
GGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTAC
GCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATAC
TTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCA
AGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAA
AATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAA
GATAACGCTGCCATTNAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTA
AAAAAGATTCTTCTGCTAAACA

---KFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSP
FVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYL
KDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQD
NAAIXFKLPSSQTDDNVNLKKILLLN

Frame A:
---eipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*sfs
ic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*il
krr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr
*rchxiqitiisn***cxskkdssak


Frame B:
---KFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSP
FVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYL
KDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQD
NAAIXFKLPSSQTDDNVNLKKILLLN


Frame C:
---nshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslvvkflh
llnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*
ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfki
tlpxnsnyhhlklmimxi*krffc*t

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK816 (VFK816Q) /CSM/VF/VFK8-A/VFK816Q.Seq.d/                       1201   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                       1201   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1201   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1201   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1201   0.0
AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/                       1201   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1201   0.0
AFO351 (AFO351Q) /CSM/AF/AFO3-C/AFO351Q.Seq.d/                       1201   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1201   0.0
AFN822 (AFN822Q) /CSM/AF/AFN8-A/AFN822Q.Seq.d/                       1201   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   166   6e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   159   4e-38
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   159   6e-38
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   157   3e-37
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   154   1e-36
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   152   7e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   149   6e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   148   1e-34
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   148   1e-34
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   148   1e-34

 psg: 0.84  gvh: 0.38  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: endoplasmic reticulum

>> prediction for AFL314 is cyt

>AFL314Z.Seq
----------AGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTT
CCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAA
ACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCT
CCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTT
GGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTAC
GCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATAC
TTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCA
AGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAA
AATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAA
GATAACGCTGCCATTNAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTA
AAAAAGATTCTTCTGCTAAACA