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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFL272
Library AF
(Link to library)
Clone ID AFL272
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFL272P
(Link to Original site)
Representative DNA sequence 
>AFL272 (AFL272Q) /CSM/AF/AFL2-C/AFL272Q.Seq.d/
ATATTAAACAACTNAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAA
AAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGG
TATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGT
TGGTCGTCCAANACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGG
TGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTAT
TGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCG
NGTTGCTCCAGGGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAA
TAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGC
CATTCAAGCTGTATTATCACTCTATGCCTCTGXXXXXXXXXXCGTACCACTGGTATCGTT
ATGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACC
ACATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAAT
CTTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATAT
TAAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTC
ATCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAA
TGAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGC
TGGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGA
TTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCG
TATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACC
ACCAGAACGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTT
CCAACAAATGGGATTTCAAAAGAAGAATATNANAATCCGGTCCATCAATGTTCACAGAAA
ATGTTTCTAAANATTAGAAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
y*ttxrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIV
GRPXHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELR
VAPGEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS---

---VPLVSLWIQVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTT
AEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQ
PSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPS
TMKIKIIAPPERKYSVWIGGSILASLSTFQQMGFQKKNXXIRSINVHRKCF*xlek
Translated Amino Acid sequence (All Frames) 
Frame A:
ilnnxkiliif*y*inkqikkkikngwxrcssfsy**wfryv*swfcw**cstccfpinc
wssxthwcygwygskgficr**ssikkryfnt*vsn*twychqlg*ygknlasyfl**ts
xcsrgtsssxn*ssiksksk*rendsnhv*nfqhssnvcchssciitlcl---

---rttgivmdsgdgvshtvpiyeglcittcyftfrfsws*sy*lhdenln*twllihnn
c*krnr*ry*reisircigf*s*nancsliicireii*itrwssyynw**tfplsrstfp
tiilgygvcwys*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctcti
nnen*nycttrt*ilcmdwwfnfsftlnfptngiskeeyxnpvhqcsqkmflxirk

Frame B:
y*ttxrf*lyfnik*ink*kkk*KMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIV
GRPXHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELR
VAPGEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS---

---VPLVSLWIQVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTT
AEREIVRDIKEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQ
PSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPS
TMKIKIIAPPERKYSVWIGGSILASLSTFQQMGFQKKNXXIRSINVHRKCF*xlek

Frame C:
ikqlkdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshqll
vvqxtlvlwlvwvkrihmsvmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnfx
llqgniqfx*lklh*iqkqierk*lksclklstlqqcmlpfklyyhsmpl---

---yhwyrygfr*wcfpyctnlrrvmhyhmlfyv*i*lvvillit**ks*lnvvthsqql
lkeksleilkrn*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnvsvvqkhfsn
hhswvwsllvfmkqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhq
q*klkllhhqnvntlyglvvqf*lhsqlsnkwdfkrrixxsgpsmftenvskx*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL272 (AFL272Q) /CSM/AF/AFL2-C/AFL272Q.Seq.d/                       2177   0.0
SFD813 (SFD813Q) /CSM/SF/SFD8-A/SFD813Q.Seq.d/                       2131   0.0
SFL261 (SFL261Q) /CSM/SF/SFL2-C/SFL261Q.Seq.d/                       2121   0.0
SFD319 (SFD319Q) /CSM/SF/SFD3-A/SFD319Q.Seq.d/                       2121   0.0
CFC565 (CFC565Q) /CSM/CF/CFC5-C/CFC565Q.Seq.d/                       2115   0.0
SFG742 (SFG742Q) /CSM/SF/SFG7-B/SFG742Q.Seq.d/                       2107   0.0
CHD357 (CHD357Q) /CSM/CH/CHD3-C/CHD357Q.Seq.d/                       2107   0.0
CFI221 (CFI221Q) /CSM/CF/CFI2-A/CFI221Q.Seq.d/                       2107   0.0
AFM847 (AFM847Q) /CSM/AF/AFM8-B/AFM847Q.Seq.d/                       2105   0.0
AFB839 (AFB839Q) /CSM/AF/AFB8-B/AFB839Q.Seq.d/                       2099   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.    928   0.0    5
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   888   0.0    18
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   793   0.0    17
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      785   0.0    5
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   785   0.0    21
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.    777   0.0    5
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   753   0.0    14
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         706   0.0    5
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   706   0.0    7
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   690   0.0    16
dna update 2004. 8.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   662   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   658   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   656   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        656   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   655   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   655   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          645   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   645   0.0
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   643   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   639   0.0
protein update 2009. 4.25
PSORT 

 psg: 0.75  gvh: 0.39  alm: 0.30  top: 0.57  tms: 0.07  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFL272 is cyt
5' end seq. ID AFL272F
5' end seq. 
>AFL272F.Seq
ATATTAAACAACTNAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAA
AAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGG
TATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGT
TGGTCGTCCAANACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGG
TGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTAT
TGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCG
NGTTGCTCCAGGGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAA
TAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGC
CATTCAAGCTGTATTATCACTCTATGCCTCTG----------
Length of 5' end seq. 512
3' end seq. ID AFL272Z
3' end seq. 
>AFL272Z.Seq
----------CGTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACTGT
ACCAATTTACGAAGGGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGTC
GTGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACAA
CTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGATT
TTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTAC
CAGATGGTCAAGTTATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTCC
AACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATCA
TGAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTA
CAACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCAT
CAACAATGAAAATTAAAATTATTGCACCACCAGAACGTAAATACTCTGTATGGATTGGTG
GTTCAATTTTAGCTTCACTCTCAACTTTCCAACAAATGGGATTTCAAAAGAAGAATATNA
NAATCCGGTCCATCAATGTTCACAGAAAATGTTTCTAAANATTAGAAAAAA
Length of 3' end seq. 701
Connected seq. ID AFL272P
Connected seq. 
>AFL272P.Seq
ATATTAAACAACTNAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAA
AAAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGG
TATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGT
TGGTCGTCCAANACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGG
TGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTAT
TGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCG
NGTTGCTCCAGGGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAA
TAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGC
CATTCAAGCTGTATTATCACTCTATGCCTCTG----------CGTACCACTGGTATCGTT
ATGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACC
ACATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAAT
CTTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATAT
TAAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTC
ATCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAA
TGAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGC
TGGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGA
TTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCG
TATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACC
ACCAGAACGTAAATACTCTGTATGGATTGGTGGTTCAATTTTAGCTTCACTCTCAACTTT
CCAACAAATGGGATTTCAAAAGAAGAATATNANAATCCGGTCCATCAATGTTCACAGAAA
ATGTTTCTAAANATTAGAAAAAA
Length of connected seq. 1213
Full length Seq ID -
Full length Seq. -
Length of full length seq. -