>SSF470 (SSF470Q) /CSM/SS/SSF4-C/SSF470Q.Seq.d/
ATAGTAATGAAGAAGAAGAAACGAATTCTGGGGATAATTCAAAAAACAATAGCAATAATT
ATTATTATTATTTAAAAAAAAAAAAAAAAAAAAATTATTTCAATATTATTGGTCAATTAA
TTTAAAGTAATATTACAACAAAAAATTTCTCAATTCAATCATACACACATTATTATTAAA
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAA

SNEEEETNSGDNSKNNSNNYYYYLKKKKKKNYFNIIGQLI*snittknfsiqsythyy*n
skkkkkkkkkkkkkkek

Frame A:
ivmkkkkrilgiiqktiaiiiiii*kkkkkkiisillvn*fkvilqqkisqfnhthiiik
ipkkkkkkkkkkkkkkkk


Frame B:
***rrrnefwg*fkkq*q*llllfkkkkkkklfqyywsinlk*yynkkflnsiihtlllk
fqkkkkkkkkkkkkkrkk


Frame C:
SNEEEETNSGDNSKNNSNNYYYYLKKKKKKNYFNIIGQLI*snittknfsiqsythyy*n
skkkkkkkkkkkkkkek

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSF470 (SSF470Q) /CSM/SS/SSF4-C/SSF470Q.Seq.d/                        180   6e-45
SHJ619 (SHJ619Q) /CSM/SH/SHJ6-A/SHJ619Q.Seq.d/                         42   0.004
CHJ328 (CHJ328Q) /CSM/CH/CHJ3-B/CHJ328Q.Seq.d/                         42   0.004
VSE441 (VSE441Q) /CSM/VS/VSE4-B/VSE441Q.Seq.d/                         40   0.014
VSC321 (VSC321Q) /CSM/VS/VSC3-A/VSC321Q.Seq.d/                         40   0.014
SSM720 (SSM720Q) /CSM/SS/SSM7-A/SSM720Q.Seq.d/                         40   0.014
SSF513 (SSF513Q) /CSM/SS/SSF5-A/SSF513Q.Seq.d/                         40   0.014
SLI707 (SLI707Q) /CSM/SL/SLI7-A/SLI707Q.Seq.d/                         40   0.014
SLA852 (SLA852Q) /CSM/SL/SLA8-C/SLA852Q.Seq.d/                         40   0.014
VSK259 (VSK259Q) /CSM/VS/VSK2-C/VSK259Q.Seq.d/                         38   0.056

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC012117|AC012117.3 Homo sapiens chromosome 4 clone RP11-362G23 map 4, WORKING DRAFT SEQUENCE, 27 unordered pieces.    60  3e-04  2
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    40  8e-04  3
AC093663|AC093663.4 Homo sapiens BAC clone RP11-477G18 from 4, complete sequence.    60  8e-04  4
AC078955|AC078955.25 Homo sapiens 12 BAC RP11-543H12 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    52  0.001  3
AC145502|AC145502.1 Canis familiaris clone RP81-204N10, WORKING DRAFT SEQUENCE.    44  0.002  7
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    38  0.005  3
AC137874|AC137874.2 Mus musculus clone RP23-58F21, WORKING DRAFT SEQUENCE, 9 unordered pieces.    38  0.009  4
BX571712|BX571712.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEYP-9A1.    50  0.012  1
AL844152|AL844152.8 Zebrafish DNA sequence from clone DKEY-37L7.    48  0.014  2
AP003515|AP003515.1 Clostridium perfringens plasmid pCP13 DNA, complete sequence.    46  0.025  5

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3236559
Number of Hits to DB: 130,764,610

 psg: 0.75  gvh: -0.13  alm: 0.71  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: plasma membrane

>> prediction for SSF470 is nuc

>SSF470E.Seq
ATAGTAATGAAGAAGAAGAAACGAATTCTGGGGATAATTCAAAAAACAATAGCAATAATT
ATTATTATTATTTAAAAAAAAAAAAAAAAAAAAATTATTTCAATATTATTGGTCAATTAA
TTTAAAGTAATATTACAACAAAAAATTTCTCAATTCAATCATACACACATTATTATTAAA
ATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAA