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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SSF455
Library SS
(Link to library)
Clone ID SSF455
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14349-1
Original site URL
Representative seq. ID SSF455E
(Link to Original site)
Representative DNA sequence 
>SSF455 (SSF455Q) /CSM/SS/SSF4-C/SSF455Q.Seq.d/
CTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGT
TTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTC
TGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGA
ATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAA
AGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAA
CATTAAAAAGAATGGCCACCTCNTCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCC
AAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAAT
GTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGA
GGAACGAATTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAATAAAA
TAATCTGTATTTTCTAAAAAAAAAAAAAA
sequence update 1999. 4. 7
Translated Amino Acid sequence 
k*ktiykkclqvll*lqtafqpsti*s*VVNMVVSSTESLTIQKKSLLTPPYQLVVHSMN
SPNVSQKTNVDTLSSTINTKKKVPKRAKSVLLPGAQIPPTLKRMATSSKDSLRKACVGIQ
VEIQGTDASEVKDSCFYEKCTKI*ii*knlkkkkkkklprnefniyk***issyi*fnki
icif*kkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
lnkklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*
ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kewppxqktlyvkpvlvs
klkskvpmplkskihvsmknvpksk*feki*kkkkkknsrgtnsifinsnksphtynlik
*svfskkkk


Frame B:
*iknyi*kmssgialapncvstfndlklgrkyggiiyrisddskeiivdstlpagcsfde
ftkclpenecryvvldyqykeegaqkskicfvawcpdtanikknghlxkrlft*slcwyp
s*npryrcl*sqrfmfl*kmyqnlnnlkkfkkkkkkktpeeriqyl*ivinllihii**n
nlyflkkkk


Frame C:
k*ktiykkclqvll*lqtafqpsti*s*VVNMVVSSTESLTIQKKSLLTPPYQLVVHSMN
SPNVSQKTNVDTLSSTINTKKKVPKRAKSVLLPGAQIPPTLKRMATSSKDSLRKACVGIQ
VEIQGTDASEVKDSCFYEKCTKI*ii*knlkkkkkkklprnefniyk***issyi*fnki
icif*kkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSF455 (SSF455Q) /CSM/SS/SSF4-C/SSF455Q.Seq.d/                        837   0.0
VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        807   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        807   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        807   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        807   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        807   0.0
VSI135 (VSI135Q) /CSM/VS/VSI1-B/VSI135Q.Seq.d/                        807   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        807   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        807   0.0
VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/                        807   0.0
own update 2001.11.12
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   567  0.0  3
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   565  0.0  3
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  5e-38  1
AC108433|AC108433.2 Mus musculus clone RP23-194M19, WORKING DRAFT SEQUENCE, 49 unordered pieces.    48  0.11  2
AL603914|AL603914.3 Human DNA sequence from clone RP11-569A2 on chromosome 6.    48  0.12  1
AC024830|AC024830.2 Caenorhabditis elegans cosmid Y55F3BR, complete sequence.    48  0.12  1
AL606471|AL606471.4 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-279L1.    48  0.12  1
AC115608|AC115608.2 Dictyostelium discoideum chromosome 2 map 6061442-6097630 strain AX4, complete sequence.    36  0.18  3
AL929264|AL929264.4 Zebrafish DNA sequence from clone CH211-59C24 in linkage group 18.    44  0.24  4
BX005447|BX005447.5 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38  0.24  4
dna update 2003. 8.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   201   1e-68
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)         88   8e-24
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...    90   3e-22
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...    92   1e-21
AY635996_1(AY635996|pid:none) Monacrosporium haptotylum cofilin-...    75   1e-19
EU961226_1(EU961226|pid:none) Zea mays clone 233661 actin-depoly...    87   1e-19
BT087663_1(BT087663|pid:none) Zea mays full-length cDNA clone ZM...    86   2e-19
PDBN(1CNU)A MOL_ID: 1;MOL_ID: 1; MOLECULE: ACTOPHORIN; MOLECULE:...    73   2e-19
(Q6EUH7) RecName: Full=Actin-depolymerizing factor 1; AltName: F...    86   3e-19
AY542311_16(AY542311|pid:none) Sorghum bicolor clone SB20O07 b1-...    83   2e-18
protein update 2009. 7. 7
PSORT 

 psg: 0.75  gvh: 0.52  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.13
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for SSF455 is nuc
5' end seq. ID SSF455F
5' end seq. 
>SSF455F.Seq
CTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGT
TTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTC
TGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGA
ATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAA
AGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAA
CATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGG
TATCCAAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGA
AAAATGTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACT
CCCGAGGAACGAATTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAA
TAAAATAATCTGTATTTTCTAAAAAAAAAAAAAA
Length of 5' end seq. 574
3' end seq. ID SSF455Z
3' end seq. 
>SSF455Z.Seq
----------TGGCCACCTCTTCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAA
GTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGT
ACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGAGG
AACGAATTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAATAAAATA
ATCTGTATTTTCTAAAAAAAAAAAAAA
Length of 3' end seq. 257
Connected seq. ID SSF455P
Connected seq. 
>SSF455P.Seq
CTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGT
TTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTC
TGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGA
ATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAA
AGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAA
CATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGG
TATCCAAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGA
AAAATGTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACT
CCCGAGGAACGAATTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAA
TAAAATAATCTGTATTTTCTAAAAAAAAAAAAAA----------TGGCCACCTCTTCAAA
AGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTC
TGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAAATCTAAATAATTTGAAAAAA
TTTAAAAAAAAAAAAAAAAAAAAAACTCCCGAGGAACGAATTCAATATTTATAAATAGTA
ATAAATCTCCTCATACATATAATTTAATAAAATAATCTGTATTTTCTAAAAAAAAAAAAA
A
Length of connected seq. 831
Full length Seq ID SSF455E
Full length Seq. 
>SSF455E.Seq
CTAAATAAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGT
TTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTC
TGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGA
ATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAA
AGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAA
CATTAAAAAGAATGGCCACCTCNTCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCC
AAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAAT
GTACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGA
GGAACGAATTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAATAAAA
TAATCTGTATTTTCTAAAAAAAAAAAAAA
Length of full length seq. 569