>AFL268 (AFL268Q) /CSM/AF/AFL2-C/AFL268Q.Seq.d/
TTAGCTTTGTTTGCTAATATTGCTTTTTAATGTGAAGGTCACCCAGAACATGATNTGGTG
ATGGTGATCATGAACATGATCATGATGAATCATTTGTTAAAATCTTAGATTCTGATAATT
TCCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTTATGCCCCATGGTGCG
GTCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAACAATTATCAGCCAACA
AAAAGATTGCAATTGCAAAAGTTGATTGTACTCAACATGAACAACTCTGTAAACAAAATA
AAGTTCAAGGTTATCCAACTTTAGTTGTTTTCAAGAATGGTAAAGCTGAACCATATGAAG
GTGATAGAACCACTAAATCAATTGTTCAAACTTTAGAAGAAGAATTAAAACCAACTATTT
CAACTTTAGAATCAAATGAAGATATTGAAGAATTTAAAAAACAACACCCAATTAGTGTTG
TTGGTTTCTTTGATAATGATCATGATGATAGATTTAAATTATTTAGTGAATTAGCAGGAA
ATAATAAGAAATCAGCTXXXXXXXXXXGTCGATATGAAGAAATTCCCACAACAAGCAACT
CATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAA
GCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATC
CAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCA
AATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCA
CCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCT
CCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAG
ATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATC
ATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGAT
CATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCA
TCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACAGANAATTAAAA
TAA

sfvc*ycflm*rsprt*XGDGDHEHDHDESFVKILDSDNFHNSVSEHDVTLVMFYAPWCG
HCKTLKPLYEEAAKQLSANKKIAIAKVDCTQHEQLCKQNKVQGYPTLVVFKNGKAEPYEG
DRTTKSIVQTLEEELKPTISTLESNEDIEEFKKQHPISVVGFFDNDHDDRFKLFSELAGN
NKKSA---

---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVE
FIQDNAAIEFKLPSSQTDDNVNLKKILLLNRXLK*

Frame A:
lalfaniaf*ceghpehdxvmvimnmimmnhllks*iliisiiqflnmt*lllcfmphga
vivkl*nhymkkllnnyqptkrlqlqklivlnmnnsvnkikfkviql*lfsrmvklnhmk
vieplnqlfkl*kkn*nqlfql*nqmkilknlknntqlvllvslimimmidlnylvn*qe
iirnq---

---VDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGG
KVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKL
GEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVE
FIQDNAAIEFKLPSSQTDDNVNLKKILLLNRXLK*

Frame B:
*lcllillfnvkvtqnmxw*w*s*t*s**iic*nlrf**fp*fsf*t*rnscyvlcpmvr
sl*nfktii*rsc*tiisqqkdcncks*lyst*ttl*tk*ssrlsnfscfqew*s*ti*r
**nh*incsnfrrriktnyfnfrik*ry*ri*kttpn*ccwfl***s***i*ii**isrk
**eis---

---si*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpshsipspngskmslvv
kflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*
vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tlln
sfkitlplnsnyhhlklmimxi*krffc*txn*n

Frame C:
sfvc*ycflm*rsprt*XGDGDHEHDHDESFVKILDSDNFHNSVSEHDVTLVMFYAPWCG
HCKTLKPLYEEAAKQLSANKKIAIAKVDCTQHEQLCKQNKVQGYPTLVVFKNGKAEPYEG
DRTTKSIVQTLEEELKPTISTLESNEDIEEFKKQHPISVVGFFDNDHDDRFKLFSELAGN
NKKSA---

---ryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*
sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir
*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*i
hsr*rch*iqitiisn***cxskkdssakqxiki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFL268 (AFL268Q) /CSM/AF/AFL2-C/AFL268Q.Seq.d/                       2307   0.0
AFO351 (AFO351Q) /CSM/AF/AFO3-C/AFO351Q.Seq.d/                       1237   0.0
AFN712 (AFN712Q) /CSM/AF/AFN7-A/AFN712Q.Seq.d/                       1237   0.0
AFN128 (AFN128Q) /CSM/AF/AFN1-B/AFN128Q.Seq.d/                       1237   0.0
AFM861 (AFM861Q) /CSM/AF/AFM8-C/AFM861Q.Seq.d/                       1237   0.0
AFM249 (AFM249Q) /CSM/AF/AFM2-C/AFM249Q.Seq.d/                       1237   0.0
AFL790 (AFL790Q) /CSM/AF/AFL7-D/AFL790Q.Seq.d/                       1237   0.0
AFK831 (AFK831Q) /CSM/AF/AFK8-B/AFK831Q.Seq.d/                       1237   0.0
AFK804 (AFK804Q) /CSM/AF/AFK8-A/AFK804Q.Seq.d/                       1237   0.0
AFN659 (AFN659Q) /CSM/AF/AFN6-C/AFN659Q.Seq.d/                       1235   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   4e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
BM898120|BM898120.1 ph59g02.y1 Ostertagia ostertagi L4 pAMP1 v1 Ostertagia ostertagi cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    56   2e-08  2
BM897160|BM897160.1 ph57e02.y1 Ostertagia ostertagi L4 pAMP1 v1 Ostertagia ostertagi cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    56   3e-08  2
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   9e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   9e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U41542_7(U41542|pid:none) Caenorhabditis elegans cosmid C07A12, ...   123   9e-54
BC076090_1(BC076090|pid:none) Danio rerio proteasome (prosome, m...   112   3e-47
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   168   4e-40
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   164   7e-39
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   162   3e-38
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   161   5e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   157   9e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   4e-36
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   152   2e-35
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   4e-35

 psg: 0.75  gvh: 0.43  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.10  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for AFL268 is cyt

>AFL268F.Seq
TTAGCTTTGTTTGCTAATATTGCTTTTTAATGTGAAGGTCACCCAGAACATGATNTGGTG
ATGGTGATCATGAACATGATCATGATGAATCATTTGTTAAAATCTTAGATTCTGATAATT
TCCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTTATGCCCCATGGTGCG
GTCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAACAATTATCAGCCAACA
AAAAGATTGCAATTGCAAAAGTTGATTGTACTCAACATGAACAACTCTGTAAACAAAATA
AAGTTCAAGGTTATCCAACTTTAGTTGTTTTCAAGAATGGTAAAGCTGAACCATATGAAG
GTGATAGAACCACTAAATCAATTGTTCAAACTTTAGAAGAAGAATTAAAACCAACTATTT
CAACTTTAGAATCAAATGAAGATATTGAAGAATTTAAAAAACAACACCCAATTAGTGTTG
TTGGTTTCTTTGATAATGATCATGATGATAGATTTAAATTATTTAGTGAATTAGCAGGAA
ATAATAAGAAATCAGCT----------

>AFL268Z.Seq
----------GTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGG
TAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGA
TGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGG
TAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAA
GGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGT
TGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATT
AGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAA
TGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGA
TGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGA
ATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTGATGATAA
TGTNAATCTAAAAAAGATTCTTCTGCTAAACAGANAATTAAAATAA

>AFL268P.Seq
TTAGCTTTGTTTGCTAATATTGCTTTTTAATGTGAAGGTCACCCAGAACATGATNTGGTG
ATGGTGATCATGAACATGATCATGATGAATCATTTGTTAAAATCTTAGATTCTGATAATT
TCCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTTATGCCCCATGGTGCG
GTCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAACAATTATCAGCCAACA
AAAAGATTGCAATTGCAAAAGTTGATTGTACTCAACATGAACAACTCTGTAAACAAAATA
AAGTTCAAGGTTATCCAACTTTAGTTGTTTTCAAGAATGGTAAAGCTGAACCATATGAAG
GTGATAGAACCACTAAATCAATTGTTCAAACTTTAGAAGAAGAATTAAAACCAACTATTT
CAACTTTAGAATCAAATGAAGATATTGAAGAATTTAAAAAACAACACCCAATTAGTGTTG
TTGGTTTCTTTGATAATGATCATGATGATAGATTTAAATTATTTAGTGAATTAGCAGGAA
ATAATAAGAAATCAGCT----------GTCGATATGAAGAAATTCCCACAACAAGCAACT
CATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAA
GCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATC
CAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCA
AATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCA
CCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCT
CCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAG
ATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATC
ATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGAT
CATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCA
TCTCAAACTGATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACAGANAATTAAAA
TAA